belongs to the family of opine dehydrogenases, phylogenetic analyses indicate that TaDH is only distantly related to the opine dehydrogenases from marine invertebrates, high sequence similarity to bacterial ornithine cyclodeaminases
bivalves have evolved diverse and highly specialised strategies for surviving in hypoxic episodes including pathways that are efficient both in terms of ATP production, and in minimising H+ and toxic end product accumulation. Under these circumstances, glycogen is metabolized to pyruvate and the cytosolic NADH/NAD+ redox ratio is balanced by the reduction of pyruvate to lactate. Alternatively, NAD+ can be recycled more efficiently by coupling an amino acid to pyruvate, with formation of opines such as alanopine, tauropine, octopine, and strombine. Specimens utilizing the octopine rather than the alanopine pathway will increase energy flow rapidly, developing a major ability to counteract environmental variations. The high ratio between malate dehydrogenase/lactate dehydrogenase is due to the ability of Pinna nobilis to turn on anaerobic metabolism as a consequence of environmental or anthropogenic stresses. Anaerobic pathways are not all equivalent in terms of energy production based upon maximum rates for ATP output (lactate > octopine > alanopine = strombine)
comparisons of opine dehydrogenases activities (octopine dehydrogenase, alanopine dehydrogenase, strombine dehydrogenase, and tauropine dehydrogenase) in the adductor muscle, overview. The ODH activity in adductor muscle increases following the marine-brackish gradient, while the one of ADH, SDH and TDH decreases following the same gradient
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cloning of the cDNA by PCR. DNA encoding TaDH is amplified by PCR from Suberites domuncula cDNA library, using the primers that are designed to amplify the complete open reading frame. Expression in Escherichia coli as His-tag fusion protein. Two allelic variants are identified, which are present in the different specimens either in a homozygotic or in a heterozygotic way (length polymorphism (453 bp and 468 bp long) is detected in the second intron and the beginning of third exon).
Opine oxidoreductases in marine worms of five phyla
Comp. Biochem. Physiol. B
no activity in Amphitrite sp., no activity in Cerebratulus sp., no activity in Chaetopterus variopedatus, no activity in Clymenella torquata, no activity in Glycera sp., no activity in Hydroides sp., no activity in Lepidonotus sp., no activity in Lineus sp., no activity in Nereis sp., no activity in Phascolopsis sp., no activity in Phascolosoma sp., no activity in Priapulus sp., no activity in Themiste sp., no activity in Urechis sp.
Sato, M.; Takeuchi, M.; Kanno, N.; Nagahisa, E.; Sato, Y.
Distribution of opine dehydrogenases and lactate dehydrogenase activities in marine animals
Comp. Biochem. Physiol. B
Anthopleura japonica, Anthopleura nigrescens, Asterias amurensis, Azumapecten farreri nipponensis, Balanus cariosus, Buccinum isaotakii, Capitulum mitella, Cellana grata, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Halichondria japonica, Haliotis discus hannai, Liolophura japonica, Littorina brevicula, Meretrix lusoria, Mizuhopecten yessoensis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Aurelia aurita, no activity in Halocynthia roretzi, no activity in Hemigrapsus sanguineus, no activity in Hexagrammos otakii, no activity in Loligo bleekeri, no activity in Mytilus edulis, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pseudocardium sachalinensis, no activity in Pugettia quadridens, no activity in Stichopus japonicus, no activity in Strongylocentrotus nudus, Octopus vulgaris, Pagurus samuelis, Patiria pectinifera, Perinereis nuntia, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Solaster paxillatus, Todarodes pacificus, Tugali gigas
Opine oxidoreductases in brachiopods, bryozoans, phoronids and molluscs
Biochem. Syst. Ecol.
Anadara ovalis, Chlorostoma funebralis, Diodora cayenensis, Glottidia pyramidata, Haliotis rufescens, Laqueus californianus, no activity in Bugula neritina, no activity in Chaetopleura apiculata, no activity in Crassostrea virginica, no activity in Crepidula fornicata, no activity in Dentalium pilsbryi, no activity in Littorina littorea, no activity in Lyonsia hyalina, no activity in Membranipora tenuis, no activity in Mopalia muscosa, no activity in Mya arenaria, no activity in Nucella lapillus, no activity in Nucula proxima, no activity in Phoronis architecta, no activity in Phoronis vancouverensis, no activity in Schizoporella floridana, no activity in Solemya velum, no activity in Spisula solidissima, no activity in Urosalpinx cinerea, Tectura testudinalis, Terebratalia transversa, Turbo castanea