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Information on EC 1.4.1.23 - valine dehydrogenase (NAD+)
for references in articles please use BRENDA:EC1.4.1.23
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EC Tree 1 Oxidoreductases
1.4 Acting on the CH-NH2 group of donors
1.4.1 With NAD+ or NADP+ as acceptor
1.4.1.23 valine dehydrogenase (NAD+)
IUBMB Comments
The enzyme from Streptomyces spp. has no activity with NADP+ [cf. EC 1.4.1.8, valine dehydrogenase (NADP+)].
The enzyme appears in viruses and cellular organisms
showSynonyms
VDH, VDH2, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
VDH2
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+
the reductive amination proceeds through a sequential ordered ternary-binary mechanism. NADH binds to the enzyme first, followed by 2-oxoisovalerate and NH3, and valine is released first, followed by NAD+
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L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+
sequential ordered mechanism with non-competitive inhibition by valine
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
L-valine:NAD+ oxidoreductase (deaminating)
The enzyme from Streptomyces spp. has no activity with NADP+ [cf. EC 1.4.1.8, valine dehydrogenase (NADP+)].
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-oxoisovalerate + NH4+ + NADH
L-valine + H2O + NAD+
-
Substrates: -
Products: -
Products: -
r
L-norleucine + H2O + NAD+
2-oxocaproate + NADH + NH3
-
Substrates: 10.5% of the activity with L-valine
Products: -
Products: -
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH
-
Substrates: 43% of the activity with L-valine
Products: -
Products: -
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
Substrates: 22% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
Substrates: 116% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
Substrates: 48.2% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
Substrates: 78% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
Substrates: 100% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
Substrates: -
Products: -
Products: -
r
2-oxohexanoate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
-
Substrates: 6.3% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxohexanoate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
-
Substrates: 43.8% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxohexanoate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
-
Substrates: -
Products: -
Products: -
r
2-oxoisohexanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
Substrates: 58% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxoisohexanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
Substrates: 26.5% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxoisohexanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
Substrates: 38.1% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxoisohexanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
Substrates: 20% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxoisohexanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
Substrates: -
Products: -
Products: -
r
2-oxoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
-
Substrates: -
Products: -
Products: -
r
2-oxoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
-
Substrates: -
Products: -
Products: -
r
2-oxoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
-
Substrates: -
Products: -
Products: -
r
2-oxoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
-
Substrates: -
Products: the 4'-pro-S hydrogen atom of NADH is transferred to the substrate in the reductive-amination reaction
Products: the 4'-pro-S hydrogen atom of NADH is transferred to the substrate in the reductive-amination reaction
r
2-oxovalerate + H2O + NAD+
L-norvaline + NH3 + NADH + H+
-
Substrates: 5.0% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxovalerate + H2O + NAD+
L-norvaline + NH3 + NADH + H+
-
Substrates: 79.5% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
2-oxovalerate + H2O + NAD+
L-norvaline + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
Substrates: 68% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
Substrates: 24.5% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
Substrates: 22.3% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
Substrates: 23.2% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
Substrates: 16.6% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
Substrates: -
Products: -
Products: -
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
Substrates: 33% of the activity with L-valine
Products: -
Products: -
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
Substrates: 125% of the activity with L-valine
Products: -
Products: -
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
Substrates: 16.5% of the activity with L-valine
Products: -
Products: -
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
Substrates: 130% of the activity with L-valine
Products: -
Products: -
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
Substrates: 68.9% of the activity with L-valine
Products: -
Products: -
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
Substrates: 158% of the activity with L-valine
Products: -
Products: -
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
-
Substrates: 16.5% of the activity with L-valine
Products: reductive amination: 48.2% of the activity with 2-oxoisovalerate
Products: reductive amination: 48.2% of the activity with 2-oxoisovalerate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
-
Substrates: L-2-aminobutyrate is identical with L-alpha-aminobutyrate
Products: reductive amination: 48.2% of the activity with 2-oxoisovalerate
Products: reductive amination: 48.2% of the activity with 2-oxoisovalerate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
-
Substrates: 16.5% of the activity with L-valine
Products: reductive amination: 48.2% of the activity with 2-oxoisovalerate
Products: reductive amination: 48.2% of the activity with 2-oxoisovalerate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
-
Substrates: L-2-aminobutyrate is identical with L-alpha-aminobutyrate
Products: reductive amination: 48.2% of the activity with 2-oxoisovalerate
Products: reductive amination: 48.2% of the activity with 2-oxoisovalerate
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH
-
Substrates: very poor substrate: 1.2% of the activity with L-valine
Products: pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate
Products: pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH
-
Substrates: very poor substrate: 1.2% of the activity with L-valine
Products: reductive amination: very poor substrate, 1.3% of the activity with 2-oxoisovalerate
Products: reductive amination: very poor substrate, 1.3% of the activity with 2-oxoisovalerate
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
-
Substrates: 4.5% of the activity with L-valine
Products: -
Products: -
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
-
Substrates: 1.9% of the activity with L-valine
Products: -
Products: -
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
-
Substrates: 3% of the activity with L-valine
Products: -
Products: -
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
-
Substrates: 14.7% of the activity with L-valine
Products: -
Products: -
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
-
Substrates: 8.3% of the activity with L-valine
Products: -
Products: -
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
-
Substrates: 46.5% of the activity with L-valine
Products: 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate
Products: 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
-
Substrates: 46.5% of the activity with L-valine
Products: reductive amination: 22.3% of the activity with 2-oxoisovalerate
Products: reductive amination: 22.3% of the activity with 2-oxoisovalerate
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
Substrates: 72% of the activity with L-valine
Products: -
Products: -
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
Substrates: 34% of the activity with L-valine
Products: -
Products: -
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
Substrates: 46.5% of the activity with L-valine
Products: -
Products: -
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
Substrates: 28% of the activity with L-valine
Products: -
Products: -
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
Substrates: 29.4% of the activity with L-valine
Products: -
Products: -
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
Substrates: 22% of the activity with L-valine
Products: -
Products: -
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
-
Substrates: 36% of the activity with L-valine
Products: 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate
Products: 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate
r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
-
Substrates: 36% of the activity with L-valine
Products: reductive amination: 38.1% of the activity with 2-oxoisovalerate
Products: reductive amination: 38.1% of the activity with 2-oxoisovalerate
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
Substrates: 73% of the activity with L-valine
Products: -
Products: -
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
Substrates: 52.8% of the activity with L-valine
Products: -
Products: -
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
Substrates: 36% of the activity with L-valine
Products: -
Products: -
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
Substrates: 8% of the activity with L-valine
Products: -
Products: -
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
Substrates: 24.5% of the activity with L-valine
Products: -
Products: -
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
Substrates: 24% of the activity with L-valine
Products: -
Products: -
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
-
Substrates: 16% of the activity with L-valine
Products: -
Products: -
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
-
Substrates: 18.5% of the activity with L-valine
Products: -
Products: -
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
-
Substrates: 10.5% of the activity with L-valine
Products: -
Products: -
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
-
Substrates: 51.5% of the activity with L-valine
Products: -
Products: -
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
-
Substrates: 34.5% of the activity with L-valine
Products: -
Products: -
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
-
Substrates: 44% of the activity with L-valine
Products: -
Products: -
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
-
Substrates: 100% of the activity with L-valine
Products: -
Products: -
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
-
Substrates: 43.0% of the activity with L-valine
Products: -
Products: -
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
-
Substrates: 98% of the activity with L-valine
Products: -
Products: -
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
-
Substrates: 96% of the activity with L-valine
Products: -
Products: -
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
Substrates: -
Products: -
Products: -
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L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
Substrates: NAD+
Products: NADH
Products: NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
Substrates: NAD+
Products: 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
Products: 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
Substrates: NAD+
Products: highest activity with 2-oxoisovalerate
Products: highest activity with 2-oxoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
Substrates: NAD+
Products: NADH
Products: NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
Substrates: NAD+
Products: 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
Products: 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
Substrates: NAD+
Products: highest activity with 2-oxoisovalerate
Products: highest activity with 2-oxoisovalerate
r
pyruvate + H2O + NAD+
L-alanine + NH3 + NADH + H+
-
Substrates: 20.8% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
pyruvate + H2O + NAD+
L-alanine + NH3 + NADH + H+
-
Substrates: 1.3% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
pyruvate + H2O + NAD+
L-alanine + NH3 + NADH + H+
-
Substrates: 23.3% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
pyruvate + NH3 + NADH + H+
L-alanine + H2O + NAD+
-
Substrates: 31.6% of the activity with 2-oxoisovalerate
Products: -
Products: -
r
pyruvate + NH3 + NADH + H+
L-alanine + H2O + NAD+
-
Substrates: -
Products: -
Products: -
r
additional information
?
-
-
Substrates: no substrates: D-valine, glycine, L-threonine, L-serine, L-cysteine, L-glutamate, L-aspartate, L-asparagine, L-arginine, L-histidine, L-tryptophan, and DL-phenylalanine, and 2-ketoglutarate and oxalacetate
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrates: D-valine, D-leucine, D-isoleucine, glycine, L-threonine, fialanine, L-serine, L-cysteine, L-methionine, L-glutamic acid, L-aspartic acid, L-asparagine, L-glutamine, L-lysine, L-phenylalanine, L-tyrosine, L-histidine and L-tryptophan as well as 2-oxoglutarate, oxalacetate, phenylpyruvate and glyoxalate
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
nicotinamide guanine dinucleotide
-
72.3% of the activity with NAD+
additional information
-
no cofactors: deamido-NAD+, 3-pyridinealdehyde-NAD+, thionicotinamide-NAD+
-
additional information
-
no cofactor: 3-acetylpyridine-NAD+, thionicotinamide-NAD+, alpha-NAD+, 3-pyridinaldehyde-NAD+, deamido-NAD+
-
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
not inhibitory at 1 mM: ATP, ADP, AMP, GTP, GDP, MgSO4, MgCl2, ZnCl2, COSO4, BaCl2, EDTA and o-phenanthroline
-
additional information
-
no inhibition by nucleotide mono-, di-, triphosphates or the free bases adenine, cytosine, thymine, guanine and uracil
-
additional information
-
activity is not significantly affected by 1 mM ATP, ADP, AMP, Mg2+, Ca2+, Cu2+, Zn2+, and metabolites of the citric acid cycle
-
additional information
-
not inhibitory: EDTA, benzamidine, PMSF, all at 5 mM, or citrate, cobalt(II), azide, Li+ and Mn2+, at 1 mM
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.2
2-oxobutanoate
-
pH 10.4, temperature not specified in the publication
0.42
2-Oxoisohexanoate
-
pH 10.4, temperature not specified in the publication
0.46
2-oxoisovalerate
-
pH 10.4, temperature not specified in the publication
1.06
3-methyl-2-oxopentanoate
-
pH 10.4, temperature not specified in the publication
4.35
L-2-aminobutanoate
-
pH 10.4, temperature not specified in the publication
333
L-alanine
-
pH 10.4, temperature not specified in the publication
1.72
L-isoleucine
-
pH 10.4, temperature not specified in the publication
1.85
L-leucine
-
pH 10.4, temperature not specified in the publication
22.5
L-norleucine
-
pH 10.4, temperature not specified in the publication
1.66
L-norvaline
-
pH 10.4, temperature not specified in the publication
0.75
L-valine
-
pH 10.4, temperature not specified in the publication
17.2
pyruvate
-
pH 10.4, temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10.4
10.7
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
65
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
a UV mutant derived from the high-producing strain 84/25
-
-
brenda
a UV mutant derived from the high-producing strain 84/25
-
-
brenda
Highest Expressing Human Cell Lines
Cell Line Links Show AA Sequence and Transmembrane Helices (510 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
116000 - 118000
-
gel filtration and equilibrium ultracentrifugation
41000
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
dodecamer
hexamer
tetramer
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, pH 7.4, presence of 2-mercaptoethanol and 30% glycerol, loss of 90% of activity with 24 h
-
-25°C, 0.2 M Tris-HCl buffer, pH 7.4, 2 months, no loss of activity
-
4°C, pH 7.4, presence of 2-mercaptoethanol and 10% glycerol, stable for 48 h
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
activity is induced in presence of L-valine
activity is repressed in presence of ammonia
activity is repressed in the presence of glucose and NH4+ or glycerol and NH4+ or asparagine
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Vancura, A.; Vancurova, I.; Volc, J.; Fussey, S.P.M.; Flieger, M.; Neuzil, J.; Marsalek, J.; Behal, V.
Valine dehydrogenase from Streptomyces fradiae: purification and properties
J. Gen. Microbiol.
134
3213-3219
1988
Streptomyces fradiae
brenda
Navarrete, R.M.; Vara, J.A.; Hutchinson, C.R.
Purification of an inducible L-valine dehydrogenase of Streptomyces coelicolor A3(2)
J. Gen. Microbiol.
136
273-281
1990
Streptomyces coelicolor
brenda
Priestley, N.D.; Robinson, J.A.
Purification and catalytic properties of L-valine dehydrogenase from Streptomyces cinnamonensis
Biochem. J.
261
853-861
1989
Streptomyces virginiae
brenda
Vancurova, I.; Vancura, A.; Volc, J.; Neuzil, J.; Flieger, M.; Basarova, G.; Behal, V.
Isolation and characterization of valine dehydrogenase from Streptomyces aureofaciens
J. Bacteriol.
170
5192-5196
1988
Kitasatospora aureofaciens, Kitasatospora aureofaciens 50/137
brenda
Nguyen, L.T.; Nguyen, K.T.; Kopecky, J.; Nova, P.; Novotna, J.; Behal, V.
Purification and characterization of a novel valine dehydrogenase from Streptomyces aureofaciens
Biochim. Biophys. Acta
1251
186-190
1995
Kitasatospora aureofaciens, Kitasatospora aureofaciens BS-5
brenda
Nguyen, L.T.; Nguyen, K.T.; Spizek, J.; Behal, V.
The tylosin producer, Streptomyces fradiae, contains a second valine dehydrogenase
Microbiology
141
1139-1145
1995
Streptomyces fradiae
-
brenda
Ohshima, T.; Soda, K.
Valine dehydrogenase from a non-spore-forming bacterium, Alcaligenes faecalis: purification and characterization
Biochim. Biophys. Acta
1162
221-226
1993
Alcaligenes faecalis
brenda
EXTERNAL LINKS (specific for EC-Number 1.4.1.23)
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