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EC Tree
IUBMB Comments Highly specific for 4-hydroxynon-2-enal and non-2-enal. Alk-2-enals of shorter chain have lower affinities. Exhibits high activities also for alk-2-enones such as but-3-en-2-one and pent-3-en-2-one. Inactive with cyclohex-2-en-1-one and 12-oxophytodienoic acid. Involved in the detoxication of alpha,beta-unsaturated aldehydes and ketones [cf. EC 1.3.1.102, 2-alkenal reductase (NADP+)].
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
2-alkenal reductase, 2AER, AER, alkenal/one oxidoreductase, alkenal/one reductase, AOR, At-AER,
At5g16970 , AtAOR, bifunctional LTB4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase,
more
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alkenal/one oxidoreductase
alkenal/one reductase
-
-
bifunctional LTB4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase
-
a beta-NADPH-dependent alkenal/one oxidoreductase which has a role for leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandinDELTA13-reductase
CS-670 double-bond reductase
-
-
double bond reductase
-
-
NADPH alkenal/one oxidoreductase
-
-
NADPH-dependent alkenal/one oxidoreductase
-
-
alkenal/one oxidoreductase
-
-
alkenal/one oxidoreductase
-
alkenal/one oxidoreductase
-
-
AOR
-
-
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a n-alkanal + NAD(P)+ = an alk-2-enal + NAD(P)H + H+
a n-alkanal + NAD(P)+ = an alk-2-enal + NAD(P)H + H+
-
-
-
-
a n-alkanal + NAD(P)+ = an alk-2-enal + NAD(P)H + H+
catalytic and kinetic mechanism
-
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n-alkanal:NAD(P)+ 2-oxidoreductase
Highly specific for 4-hydroxynon-2-enal and non-2-enal. Alk-2-enals of shorter chain have lower affinities. Exhibits high activities also for alk-2-enones such as but-3-en-2-one and pent-3-en-2-one. Inactive with cyclohex-2-en-1-one and 12-oxophytodienoic acid. Involved in the detoxication of alpha,beta-unsaturated aldehydes and ketones [cf. EC 1.3.1.102, 2-alkenal reductase (NADP+)].
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(+)-acylfulvene + NADPH
?
-
-
-
-
?
(+)-hydroxymethylacylfulvene + NADPH
?
-
-
-
-
?
(-)-acylfulvene + NADPH
?
-
-
-
-
?
(-)-hydroxymethylacylfulvene + NADPH
?
-
-
-
-
?
(2E),(6Z)-nonadienal + NADPH
?
-
-
-
-
?
(2E)-4-hydroxynon-2-enal + NADPH + H+
4-hydroxynonanal + NADP+
-
-
-
-
?
(2E)-hexenal + NAD(P)H
n-hexanal + NAD(P)+
-
-
-
?
(2E)-nonenal + NAD(P)H
n-nonanal + NAD(P)+
-
-
-
-
?
(2E)-pentenal + NAD(P)H
n-pentanal + NAD(P)+
-
-
-
-
?
1-penten-3-one + NADH
? + NAD+
1-penten-3-one + NADH
pentan-3-one + NAD+
-
-
-
?
1-penten-3-one + NADPH
? + NADP+
-
-
-
?
1-penten-3-one + NADPH
pentan-3-one + NADP+
13-oxo-(9E,11Z)-octadecadienoic acid + NADPH
?
-
-
-
-
?
15-deoxy-DELTA12,14-prostaglandin + NADPH
?
-
-
-
-
?
15-deoxy-DELTA12,14-prostaglandin J2 + NADPH + H+
12,13-dihydro-15-deoxy-DELTA12,14-prostaglandin J2 + NADP+
-
-
-
-
r
15-oxoprostaglandin E1 + NAD(P)H
? + NAD(P)+
-
-
-
-
?
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid + NADPH
2-[4-[(2-oxocyclohexyl)methyl]phenyl]propanoic acid + NADP+
-
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid is also known as CS-670
-
-
?
2E-hexenal + NADPH + H+
hexanal + NADP+
-
at 12% of the rate with 2E-nonenal
-
-
?
2E-nonenal + NADPH + H+
nonanal + NADP+
-
-
-
-
?
3-buten-2-one + NAD(P)H
2-butanone + NAD(P)+
-
-
-
-
?
3-buten-2-one + NADH
2-butanone + NAD+
3-buten-2-one + NADPH
2-butanone + NADP+
3-nonen-2-one + NADPH
nonan-2-one
-
-
-
-
?
3-penten-2-one + NAD(P)H
2-pentanone + NAD(P)+
-
-
-
-
?
3-penten-2-one + NADH
2-pentanone + NAD+
-
-
-
?
3-penten-2-one + NADPH
2-pentanone + NADP+
-
-
-
?
4-hexen-3-one + NADH
hexan-3-one + NAD+
4-hexen-3-one + NADPH
hexan-3-one + NADP+
4-hydroxy-(2E)-hexenal + NAD(P)H
4-hydroxyhexenal + NAD(P)+
-
-
-
-
?
4-hydroxy-(2E)-nonenal + NAD(P)H
4-hydroxynonylaldehyde + NAD(P)+
4-hydroxy-(2E)-nonenal + NADPH + H+
4-hydroxynonanal + NADP+
-
-
-
-
?
4-oxo-(2E)-nonenal + NADPH + H+
4-oxononanal + NADP+
-
-
-
-
?
9-oxo-(10E,12Z)-octadecadienoic acid + NADPH
?
-
-
-
-
?
artemisinic aldehyde + NADPH + H+
dihydroartemisinic aldehyde + NADP+
-
at 1.9% of the rate with 2E-nonenal
88% 11S- and 12% 11R-isomer
-
?
benzylidine acetone + NAD(P)H
? + NAD(P)+
-
reduction of alpha,beta-double bond
-
-
?
crotonaldehyde + NADH
butanal + NAD+
crotonaldehyde + NADPH
butanal + NADP+
diamide + NAD(P)H
? + NAD(P)+
-
-
-
-
?
ethyl vinyl ketone + NAD(P)H
diethylketone + NAD(P)+
-
-
-
-
?
methyl vinyl ketone + NAD(P)H
dimethylketone + NAD(P)+
-
-
-
-
?
n-alkanal + NAD(P)+
alk-2-enal + NAD(P)H
propenal + NAD(P)H
propanal + NAD(P)+
propenal + NADH
propanal + NAD+
propenal + NADPH
propanal + NADP+
trans,trans-2,4-alkadienal + NAD(P)H
? + NAD(P)+
-
-
-
-
?
trans-2-alkenal + NAD(P)H
alkanal + NAD(P)+
-
-
-
-
?
trans-2-nonenal + NAD(P)H
nonanal + NAD(P)+
-
-
-
-
?
trans-2-pentenal + NADPH
pentanal + NADP+
-
-
-
-
?
trans-3-nonen-2-one + NAD(P)H
2-nonanone + NAD(P)+
-
-
-
-
?
trans-4-hydroxy-2-alkenal + NAD(P)H
4-hydroxyalkanal + NAD(P)+
-
-
-
-
?
additional information
?
-
1-penten-3-one + NADH
? + NAD+
-
-
-
-
?
1-penten-3-one + NADH
? + NAD+
-
-
-
?
1-penten-3-one + NADPH
pentan-3-one + NADP+
-
-
-
-
?
1-penten-3-one + NADPH
pentan-3-one + NADP+
-
-
-
?
3-buten-2-one + NADH
2-butanone + NAD+
-
-
-
-
?
3-buten-2-one + NADH
2-butanone + NAD+
-
-
-
?
3-buten-2-one + NADPH
2-butanone + NADP+
-
-
-
-
?
3-buten-2-one + NADPH
2-butanone + NADP+
-
-
-
?
4-hexen-3-one + NADH
hexan-3-one + NAD+
-
-
-
-
?
4-hexen-3-one + NADH
hexan-3-one + NAD+
-
-
-
?
4-hexen-3-one + NADPH
hexan-3-one + NADP+
-
-
-
-
?
4-hexen-3-one + NADPH
hexan-3-one + NADP+
-
-
-
?
4-hydroxy-(2E)-nonenal + NAD(P)H
4-hydroxynonylaldehyde + NAD(P)+
-
-
-
?
4-hydroxy-(2E)-nonenal + NAD(P)H
4-hydroxynonylaldehyde + NAD(P)+
-
-
-
-
?
crotonaldehyde + NADH
butanal + NAD+
-
-
-
-
?
crotonaldehyde + NADH
butanal + NAD+
-
-
-
?
crotonaldehyde + NADPH
butanal + NADP+
-
-
-
-
?
crotonaldehyde + NADPH
butanal + NADP+
-
-
-
?
illudin M + NADPH
?
-
-
-
-
?
illudin M + NADPH
?
-
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
-
-
?
illudin S + NADPH
?
-
-
-
-
?
illudin S + NADPH
?
-
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
-
-
?
irofulven + NADPH
?
-
-
-
-
?
irofulven + NADPH
?
-
less cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
-
-
?
n-alkanal + NAD(P)+
alk-2-enal + NAD(P)H
-
carbon chain length C3-C9 serves as substrates
-
-
?
n-alkanal + NAD(P)+
alk-2-enal + NAD(P)H
-
role in detoxication
-
-
?
n-alkanal + NAD(P)+
alk-2-enal + NAD(P)H
-
-
-
-
?
n-alkanal + NAD(P)+
alk-2-enal + NAD(P)H
-
role in detoxication
-
-
?
propenal + NAD(P)H
propanal + NAD(P)+
-
synonym: acrolein
-
-
?
propenal + NAD(P)H
propanal + NAD(P)+
-
synonym: acrolein
-
-
?
propenal + NADH
propanal + NAD+
-
-
-
-
?
propenal + NADH
propanal + NAD+
-
-
-
?
propenal + NADPH
propanal + NADP+
-
-
-
-
?
propenal + NADPH
propanal + NADP+
-
-
-
?
additional information
?
-
-
catalyzes the hydrogenation of alpha,beta-unsaturated bonds, but not the reduction of the aldehyde moiety in 2-alkenal molecules
-
-
?
additional information
?
-
-
reduction of the alpha,beta-carbon-carbon double bond to a single bond, broad substrate specificity
-
-
?
additional information
?
-
-
phase2/antioxidative enzyme
-
-
?
additional information
?
-
-
reaction product identification by mass spectrometry
-
-
?
additional information
?
-
-
regio- and stereochemistry of hydride transfer, overview
-
-
?
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illudin M + NADPH
?
-
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
-
-
?
illudin S + NADPH
?
-
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
-
-
?
irofulven + NADPH
?
-
less cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
-
-
?
n-alkanal + NAD(P)+
alk-2-enal + NAD(P)H
additional information
?
-
-
phase2/antioxidative enzyme
-
-
?
n-alkanal + NAD(P)+
alk-2-enal + NAD(P)H
-
role in detoxication
-
-
?
n-alkanal + NAD(P)+
alk-2-enal + NAD(P)H
-
role in detoxication
-
-
?
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additional information
-
no cofactor: NADH
-
NADH
-
low activity, 20fold higher activity with NADPH
NADH
-
enzyme operates 30times faster in the presence of NADPH than in the presence of NADH
NADH
enzyme operates 30times faster in the presence of NADPH than in the presence of NADH
NADPH
-
-
NADPH
-
dependent on, beta-specific
NADPH
-
enzyme operates 30times faster in the presence of NADPH than in the presence of NADH
NADPH
enzyme operates 30times faster in the presence of NADPH than in the presence of NADH
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Ca2+
-
stimulates activity
Li+
-
stimulates activity
Mg2+
-
stimulates activity
Mn2+
-
stimulates activity
Na+
-
stimulates activity
additional information
-
enzyme contains no Zn2+
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15-keto prostaglandin F2alpha
-
43.06% residual activity at 0.1 mM
2-nonanone
-
product inhibition, competitive versus trans-3-nonen-2-one
benzylidene acetophenone
-
21.09% residual activity at 0.5 mM
dicoumarol
-
11.45% residual activity at 0.1 mM
indomethacin
-
14.22% residual activity at 0.1 mM
irofulven
-
recombinant human 293 cells show 100fold increased sensitivity compared to the wild-type human 293 cells
NADP+
-
product inhibition, competitive versus NADPH
quercitrin
-
13.14% residual activity at 0.1 mM
additional information
-
no inhibition by 9,10-phenanthroline
-
additional information
-
finasteride has no inhibitory effect
-
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ammonium sulfate
-
stimulates activity
CH3COO- NH4+
-
stimulates activity
CH3COONa
-
stimulates activity
estradiol
-
the transcription of 2AER is estradiol-induced and correlates with conditional salt tolerance
Na2S2O5
-
stimulates activity
Na2SO4
-
stimulates activity
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Neoplasms
Increased expression of prostaglandin reductase 1 in hepatocellular carcinomas from clinical cases and experimental tumors in rats.
Neoplasms
NADPH alkenal/one oxidoreductase activity determines sensitivity of cancer cells to the chemotherapeutic alkylating agent irofulven.
Neoplasms
Nrf2-mediated induction of cytoprotective enzymes by 15-deoxy-Delta12,14-prostaglandin J2 is attenuated by alkenal/one oxidoreductase.
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0.465
(+)-acylfulvene
-
pH 7.2, 37°C
0.243
(+)-hydroxymethylacylfulvene
-
pH 7.2, 37°C
0.538
(-)-acylfulvene
-
pH 7.2, 37°C
0.213
(-)-hydroxymethylacylfulvene
-
pH 7.2, 37°C
0.232
(2E)-hexenal
-
pH 6
0.005
(2E)-nonenal
-
pH 6
1.42
(2E)-pentenal
-
pH 6
0.0113
(2E,6Z)-nonadienal
-
pH 6.0
0.29 - 0.58
1-penten-3-one
0.00392
13-oxo-(9E,11Z)-octadecadienoic acid
-
pH 6.0
0.0096
15-deoxy-DELTA12,14-prostaglandin J2
-
pH 7.2
0.001
15-oxoprostaglandin E1
-
30°C
0.02
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid
-
at 30°C
0.0064
2E-nonenal
-
pH 7.0, 30°C
0.055 - 2.88
3-buten-2-one
0.000673
3-nonen-2-one
-
pH 6.0
0.0052 - 0.63
3-penten-2-one
0.023 - 0.12
4-hexen-3-one
0.145
4-hydroxy-(2E)-hexenal
-
pH 6
0.0134
4-hydroxy-(2E)-nonenal
0.12
4-hydroxy-2-nonenal
-
30°C
0.00124
4-oxo-(2E)-nonenal
-
pH 6.0
0.01
9-oxo-(10E,12Z)-octadecadienoic acid
-
pH 6.0
0.071
artemisinic aldehyde
-
pH 7.0, 30°C
1.8 - 29.8
crotonaldehyde
0.11
ethyl vinyl ketone
-
30°C
0.109
illudin M
-
recombinant enzyme, pH 7.4, 37°C
0.308
illudin S
-
recombinant enzyme, pH 7.4, 37°C
0.145
irofulven
-
recombinant enzyme, pH 7.4, 37°C
0.078
trans-2-nonenal
-
recombinant enzyme, pH 7.2, 25°C
0.24
trans-2-pentenal
-
pH 7.2
0.028
trans-3-nonen-2-one
-
recombinant enzyme, pH 7.2, 25°C
additional information
additional information
-
ordered Theorell-Chance kinetic mechanism, steady-state kinetics, detailed analysis, kinetic isotopic effects
-
0.29
1-penten-3-one
-
pH 7.0, 25°C
0.58
1-penten-3-one
pH 7.0, 25°C
0.055
3-buten-2-one
-
pH 6
0.13
3-buten-2-one
-
pH 7.0, 25°C
2.88
3-buten-2-one
pH 7.0, 25°C
0.0052
3-penten-2-one
-
pH 6
0.63
3-penten-2-one
pH 7.0, 25°C
0.023
4-hexen-3-one
pH 7.0, 25°C
0.12
4-hexen-3-one
-
pH 7.0, 25°C
0.0134
4-hydroxy-(2E)-nonenal
-
pH 6.0
0.0134
4-hydroxy-(2E)-nonenal
-
pH 6
1.8
crotonaldehyde
-
pH 7.0, 25°C
29.8
crotonaldehyde
pH 7.0, 25°C
0.053
NADH
-
pH 7.0, 25°C
0.004
NADPH
-
recombinant enzyme, pH 7.2, 25°C, with substrate trans-2-nonenal
0.01
NADPH
-
pH 7.0, 25°C
0.032
NADPH
-
recombinant enzyme, pH 7.2, 25°C, with substrate trans-3-nonen-2-one
0.185
NADPH
pH 7.0, 25°C
3.5
propenal
-
pH 7.0, 25°C
13.6
propenal
pH 7.0, 25°C
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0.583
(2E)-pentenal
-
pH 6
33.9
(2E,6Z)-nonadienal
-
pH 6.0
1.1
13-oxo-(9E,11Z)-octadecadienoic acid
-
pH 6.0
0.308
15-deoxy-DELTA12,14-prostaglandin J2
-
pH 7.2
40
15-oxoprostaglandin E1
-
30°C
18.9
3-nonen-2-one
-
pH 6.0
0.7
4-hydroxy-(2E)-hexenal
-
pH 6
1.47 - 88.1
4-hydroxy-(2E)-nonenal
66.7
4-hydroxy-2-nonenal
-
30°C
20.1
4-oxo-(2E)-nonenal
-
pH 6.0
0.94
9-oxo-(10E,12Z)-octadecadienoic acid
-
pH 6.0
160
ethyl vinyl ketone
-
30°C
0.97 - 16.83
trans-2-pentenal
4
1-penten-3-one
-
pH 7.0, 25°C
61
1-penten-3-one
pH 7.0, 25°C
1.38
3-buten-2-one
-
pH 6
3.5
3-buten-2-one
-
pH 7.0, 25°C
74
3-buten-2-one
pH 7.0, 25°C
1.72
3-penten-2-one
-
pH 6
22
3-penten-2-one
pH 7.0, 25°C
3.3
4-hexen-3-one
-
pH 7.0, 25°C
68
4-hexen-3-one
pH 7.0, 25°C
1.47
4-hydroxy-(2E)-nonenal
-
pH 6
20 - 50
4-hydroxy-(2E)-nonenal
-
pH 6.0
88.1
4-hydroxy-(2E)-nonenal
-
pH 6.0
1.3
crotonaldehyde
-
pH 7.0, 25°C
42
crotonaldehyde
pH 7.0, 25°C
23
NADH
-
pH 7.0, 25°C
23
NADPH
-
pH 7.0, 25°C
0.667
propenal
-
pH 6
22
propenal
-
pH 7.0, 25°C
63
propenal
pH 7.0, 25°C
0.97
trans-2-pentenal
-
pH 7.2
16.83
trans-2-pentenal
-
pH 7.2
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13.8 - 105
1-penten-3-one
25.7 - 26.9
3-buten-2-one
34.9
3-penten-2-one
pH 7.0, 25°C
27.5 - 2960
4-hexen-3-one
13.8
1-penten-3-one
-
pH 7.0, 25°C
105
1-penten-3-one
pH 7.0, 25°C
25.7
3-buten-2-one
pH 7.0, 25°C
26.9
3-buten-2-one
-
pH 7.0, 25°C
27.5
4-hexen-3-one
-
pH 7.0, 25°C
2960
4-hexen-3-one
pH 7.0, 25°C
16.2
NADH
pH 7.0, 25°C
406
NADPH
pH 7.0, 25°C
2300
NADPH
-
pH 7.0, 25°C
4.63
propenal
pH 7.0, 25°C
6.28
propenal
-
pH 7.0, 25°C
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additional information
additional information
-
product inhibition kinetics
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.275
-
purified recombinant enzyme, substrate irofulven
0.803
-
after 150fold purification
115.9
-
purified recombinant enzyme, substrate illudin S
44.1
-
purified recombinant enzyme, substrate illudin M
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.3 - 6.6
-
50% activity at pH 5.3 and pH 6.6
additional information
-
pH-profile
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line N180
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chloroplast variant
UniProt
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cytosolic variant
UniProt
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developed silique
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additional information
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Dbr1 expression is readily detectable in aerial parts of the plant, but not in the roots
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rosette leaf, caulette leaf, wilted leaf
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At-AER protein fused with the Aequorea victoria green fluorescent protein localizes in cytosol and the nucleus in Bright-Yellow 2 cells of tabacco
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At-AER protein fused with the Aequorea victoria green fluorescent protein localizes in cytosol and the nucleus in Bright-Yellow 2 cells of tobacco
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malfunction
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leaf aldehyde compositions in the AER-overproducing tobaccos and wild type leaves are analyzed. In wild-tpye leaves, 2-alkenals such as (E)-2-pentenal, acrolein, and (E)-2-hexenal are increased by 70-290% after 30 min-illumination. In the leaves of AER-tobaccos, light-dependent increase of these 2-alkenals is lower
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135000
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trimeric form, gel filtration
38500
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x * 38500, calculated
90000
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dimeric form, gel filtration
42000
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SDS-PAGE
45000
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2 * 45000, SDS-PAGE, enzyme can form dimeric and trimeric aggretates
45000
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3 * 45000, SDS-PAGE, enzyme can form dimeric and trimeric aggretates
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x * 38500, calculated
dimer
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2 * 45000, SDS-PAGE, enzyme can form dimeric and trimeric aggretates
trimer
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3 * 45000, SDS-PAGE, enzyme can form dimeric and trimeric aggretates
monomer
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-80°C, no loss of activity for 8 months
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2',5'-ADP Sepharose 4B column chromatography, Resource Q column chromatography, and HiLoad Superdex gel filtration
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using anion exchange chromatography, hydroxyapatite, phenyl-Sepharose, and gel filtration
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homogeneity
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using anion exchange chromatography, hydroxyapatite, phenyl-Sepharose, and gel filtration
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using anion exchange chromatography, hydroxyapatite, phenyl-Sepharose, and gel filtration
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expressed in Escherichia coli Top10 cells
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expression in Escherichia coli
heterologous expression of At-AER in tobacco suppresses the damages of leaf tissue by the exogenously added reactive carbonyl 4-hydroxy-(2E)-nonenal. The leaves of At-AER overproducing tobaccos suffer less damage caused by photooxidative stress due to MV/light or to intense light only
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human embryonic kidney cells (293 cell line) are transiently transfected either with an episomal rAOR overexpression vector (pCEP4-rAOR) or control vector (pCEP4). AOR transfected cells produce a 7-8fold higher expression of AOR than control cells
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in transgenic tobaccos that overexpress the AER gene
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overexpressed in Escherichia coli as a GST-fusion protein
overexpression in human 293 cells increases their sensitivity to irofulven by 100fold compared to control human 293 cells
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expression in Escherichia coli
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expression in Escherichia coli
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overexpressed in Escherichia coli as a GST-fusion protein
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overexpressed in Escherichia coli as a GST-fusion protein
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Stoffel, W.; Därr, W.
Purification and characterization of 2-alkenal reductase
Hoppe-Seyler's Z. Physiol. Chem.
356
385-390
1975
Rattus norvegicus
brenda
Dick, R.A.; Kwak, M.K.; Sutter, T.R.; Kensler, T.W.
Antioxidative function and substrate specificity of NAD(P)H-dependent alkenal/one oxidoreductase. A new role for leukotriene B4 12-hydroxydehydrogenase/15-oxoprostaglandin 13-reductase
J. Biol. Chem.
276
40803-40810
2001
Rattus norvegicus
brenda
Mano, J.i.; Torii, Y.; Hayashi, S.i.; Takimoto, K.; Matsui, K.; Nakamura, K.; Inze, D.; Babiychuk, E.; Kushnir, S.; Asada, K.
The NADPH:quinone oxidoreductase P1-x-crystallin in Arabidopsis catalyzes the alpha,beta-hydrogenation of 2-alkenals: Detoxication of the lipid peroxide-derived reactive aldehydes
Plant Cell Physiol.
43
1445-1455
2002
Arabidopsis thaliana
brenda
Dick, R.A.; Yu, X.; Kensler, T.W.
NADPH alkenal/one oxidoreductase activity determines sensitivity of cancer cells to the chemotherapeutic alkylating agent irofulven
Clin. Cancer Res.
10
1492-1499
2004
Rattus norvegicus
brenda
Dick, R.A.; Kensler, T.W.
The catalytic and kinetic mechanisms of NADPH-dependent alkenal/one oxidoreductase
J. Biol. Chem.
279
17269-17277
2004
Rattus norvegicus
brenda
Yu, X.; Egner, P.A.; Wakabayashi, J.; Wakabayashi, N.; Yamamoto, M.; Kensler, T.W.
Nrf2-mediated induction of cytoprotective enzymes by 15-deoxy-DELTA12,14-prostaglandin J2 is attenuated by alkenal/one oxidoreductase
J. Biol. Chem.
281
26245-26252
2006
Rattus norvegicus
brenda
Gong, J.; Neels, J.F.; Yu, X.; Kensler, T.W.; Peterson, L.A.; Sturla, S.J.
Investigating the role of stereochemistry in the activity of anticancer acylfulvenes: synthesis, reductase-mediated bioactivation, and cellular toxicity
J. Med. Chem.
49
2593-2599
2006
Homo sapiens
brenda
Mano, J.; Belles-Boix, E.; Babiychuk, E.; Inze, D.; Torii, Y.; Hiraoka, E.; Takimoto, K.; Slooten, L.; Asada, K.; Kushnir, S.
Protection against photooxidative injury of tobacco leaves by 2-alkenal reductase. Detoxication of lipid peroxide-derived reactive carbonyls
Plant Physiol.
139
1773-1783
2005
Arabidopsis thaliana
brenda
Papdi, C.; Abraham, E.; Joseph, M.P.; Popescu, C.; Koncz, C.; Szabados, L.
Functional identification of Arabidopsis stress regulatory genes using the controlled cDNA overexpression system
Plant Physiol.
147
528-542
2008
Arabidopsis thaliana
brenda
Itoh, K.; Yamamoto, K.; Adachi, M.; Kosaka, T.; Tanaka, Y.
Leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandin DELTA13-reductase (LTB4 12-HD/PGR) responsible for the reduction of a double-bond of the alpha,beta-unsaturated ketone of an aryl propionic acid non-steroidal anti-inflammatory agent CS-670
Xenobiotica
38
249-263
2008
Rattus norvegicus
brenda
Zhang, Y.; Teoh, K.; Reed, D.; Covello, P.
Molecular cloning and characterization of Dbr1, a 2-alkenal reductase from Artemisia annua
Botany
87
643-649
2009
Artemisia annua
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brenda
Yamauchi, Y.; Hasegawa, A.; Taninaka, A.; Mizutani, M.; Sugimoto, Y.
NADPH-dependent reductases involved in the detoxification of reactive carbonyls in plants
J. Biol. Chem.
286
6999-7009
2011
Arabidopsis thaliana, Cucumis sativus (E2S0A5), Cucumis sativus (E2S0A6), Cucumis sativus
brenda
Mano, J.; Tokushige, K.; Mizoguchi, H.; Fujii, H.; Khorobrykh, S.
Accumulation of lipid peroxide-derived, toxic alpha, beta-unsaturated aldehydes (E)-2-pentenal, acrolein and (E)-2-hexenal in leaves under photoinhibitory illumination
Plant Biotechnol.
27
193-197
2010
Arabidopsis thaliana
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brenda
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