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IUBMB Comments The enzyme, characterized from the plant Rauvolfia serpentina , participates in the ajmaline biosynthesis pathway. It has a much lower activity on vomilenine.
Reaction Schemes
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(2R)-1,2-dihydrovomilenine
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Synonyms
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1,2(R)-dihydrovomilenine 19,20(S)-reductase
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1,2-dihydrovomilene reductase
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1,2-dihydrovomilenine 19,20(S)-reductase
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1,2-dihydrovomilenine reductase
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17-O-acetylnorajmaline:NADP+ oxidoreductase
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DHVR
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RR4
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17-O-acetylnorajmaline + NADP+ = (2R)-1,2-dihydrovomilenine + NADPH + H+
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oxidation
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reduction
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MetaCyc
ajmaline and sarpagine biosynthesis
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17-O-acetylnorajmaline:NADP+ 19,20-oxidoreductase
The enzyme, characterized from the plant Rauvolfia serpentina, participates in the ajmaline biosynthesis pathway. It has a much lower activity on vomilenine.
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(R)-1,2-dihydrovomilenine + NADPH + H+
17-O-acetylnorajmaline + NADP+
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Substrates: -
?
1,2-dihydrovomilenine + NADPH + H+
17-O-acetylnorajmaline + NADP+
17-O-acetylnorajmaline + NADP+
1,2-dihydrovomilenine + NADPH + H+
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Substrates: -
r
vomilenine + NADPH + H+
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Substrates: -
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1,2-dihydrovomilenine + NADPH + H+
17-O-acetylnorajmaline + NADP+
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Substrates: -
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1,2-dihydrovomilenine + NADPH + H+
17-O-acetylnorajmaline + NADP+
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Substrates: enzyme is involved in the late stages of the biosynthesis of ajmaline
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1,2-dihydrovomilenine + NADPH + H+
17-O-acetylnorajmaline + NADP+
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Substrates: -
?
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(R)-1,2-dihydrovomilenine + NADPH + H+
17-O-acetylnorajmaline + NADP+
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Substrates: -
?
1,2-dihydrovomilenine + NADPH + H+
17-O-acetylnorajmaline + NADP+
17-O-acetylnorajmaline + NADP+
1,2-dihydrovomilenine + NADPH + H+
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Substrates: -
r
1,2-dihydrovomilenine + NADPH + H+
17-O-acetylnorajmaline + NADP+
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Substrates: enzyme is involved in the late stages of the biosynthesis of ajmaline
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1,2-dihydrovomilenine + NADPH + H+
17-O-acetylnorajmaline + NADP+
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Substrates: -
?
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0.032
(R)-1,2-dihydrovomilenine
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N-terminally truncated enzyme, at pH 7.5 and 30°C
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1.089
Vomilenine
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N-terminally truncated enzyme, at pH 7.5 and 30°C
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10.4
(R)-1,2-dihydrovomilenine
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N-terminally truncated enzyme, at pH 7.5 and 30°C
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0.2
Vomilenine
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N-terminally truncated enzyme, at pH 7.5 and 30°C
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326
(R)-1,2-dihydrovomilenine
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N-terminally truncated enzyme, at pH 7.5 and 30°C
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0.183
Vomilenine
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N-terminally truncated enzyme, at pH 7.5 and 30°C
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additional information
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5.2 - 7.7
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50% of maximal activity at pH 5.2 and at pH 7.7
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no activity in Catharanthus roseus
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brenda
no activity in Gossypium hirsutum
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brenda
no activity in Lonicera morrowii
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brenda
no activity in Nicotiana tabacum
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brenda
no activity in Saponaria officinalis
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brenda
no activity in Solanum lycopersicum
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brenda
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brenda
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brenda
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brenda
Indian snakeroot
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brenda
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brenda
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brenda
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brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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DHVOR_RAUTE
363
0
38692
Swiss-Prot
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DHVOR_RAUSE
363
0
38466
Swiss-Prot
Secretory Pathway (Reliability: 4 )
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48000
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x * 48000, SDS-PAGE
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5.8 - 6.2
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in 50 mM citric acid, 100 mM Na2HPO4 buffer, stable
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Ni-NTA column chromatography
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expressed in Saccharomyces cerevisiae and Escherichia coli BL21(DE3) strain
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Gao, S.; Von Schumann, G.; Stockigt, J.
A newly-detected reductase from Rauvolfia closes a gap in the biosynthesis of the antiarrhythmic alkaloid ajmaline
Planta Med.
68
906-911
2002
no activity in Catharanthus roseus, no activity in Gossypium hirsutum, no activity in Lonicera morrowii, no activity in Nicotiana tabacum, no activity in Saponaria officinalis, no activity in Solanum lycopersicum, Rauvolfia mannii, Rauvolfia mombasiana, Rauvolfia serpentina
brenda
Gao, S.; von Schumann, G.; Stoeckigt, J.
Biosynthesis of monoterpenoid indole alkaloid ajmaline catalyzed by novel reductases
Chem. Res. Chin. Univ.
22
267-269
2006
Rauvolfia serpentina
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brenda
Ruppert, M.; Ma, X.; Stoeckigt, J.
Alkaloid biosynthesis in Rauvolfia-cDNA cloning of major enzymes of the ajmaline pathway
Curr. Org. Chem.
9
1431-1444
2005
Rauvolfia serpentina
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brenda
Guo, J.; Gao, D.; Lian, J.; Qu, Y.
De novo biosynthesis of antiarrhythmic alkaloid ajmaline
Nat. Commun.
15
457
2024
Rauvolfia serpentina
brenda
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