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Information on EC 1.3.1.42 - 12-oxophytodienoate reductase
for references in articles please use BRENDA:EC1.3.1.42
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EC Tree 1 Oxidoreductases
1.3 Acting on the CH-CH group of donors
1.3.1 With NAD+ or NADP+ as acceptor
1.3.1.42 12-oxophytodienoate reductase
IUBMB Comments
The enzyme catalyses the reduction of (9S,13S,15Z)-12-oxophyto-10,15-dienoate during the biosynthesis of jasmonate from α-linolenate in Zea mays.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
- 1.3.1.42
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jasmonic
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allene
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octadecanoids
- oxylipins
- cyclopentanone
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ja-deficient
- environmental protection
Reaction Schemes
showSynonyms
12-oxophytodienoate reductase, 12-oxo-phytodienoic acid reductase, csopr3, opda reductase, osopr7, taopr1, 12-oxophytodienoate reductase 3, osopr8, oprii, oxophytodienoic acid reductase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
12-oxo phytodienoic acid reductase
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12-oxo-phytodienoate acid reductase
12-oxo-phytodienoic acid reductase
12-oxophytodienoate reductase 1
12-oxophytodienoate reductase 3
12-oxophytodienoate-10,11-reductase
12-oxophytodienoate10,11-reductase
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morphine reductase
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the enzyme is closely related to the isoforms of old yellow enzyme with major differences in the kinetic properties
old yellow enzyme
OPDA reductase
OPR
OPR1
OPR2
OPR3
OPR4
OPR5
OPR6
OPR7
OPRI
oxophytodienoate reductase
phytodienoic acid reductase
S64
additional information
isozymes belong to subgroup I of the 12-oxophytodienoate reductase enzyme family
12-oxo-phytodienoic acid reductase
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-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NADP+ = (15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NADP+ = (15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
involved in the conversion of linolenate into jasmonate in Zea mays
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8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NADP+ = (15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
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8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NADP+ = (15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
catalyzes the reduction of natural (+)-cis-12-oxophytodienoate for the jasmonic acid biosynthesis
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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-
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redox reaction
reduction
redox reaction
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reduction
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PATHWAY SOURCE
PATHWAYS
MetaCyc
jasmonic acid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate:NADP+ 4-oxidoreductase
The enzyme catalyses the reduction of (9S,13S,15Z)-12-oxophyto-10,15-dienoate during the biosynthesis of jasmonate from alpha-linolenate in Zea mays.
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CAS REGISTRY NUMBER
COMMENTARY
101150-03-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(15Z)-12-oxophyto-10,15-dienoate + NADH + H+
8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NAD+
-
Substrates: -
Products: -
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r
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
(15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: -
Products: -
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?
(15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NADP+
Substrates: -
Products: -
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r
(2Z)-2-methylbut-2-enedioic acid + NADH
(2R)-2-methylbutanedioic acid + NAD+
Substrates: above 99% conversion with OPR-1
Products: -
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(2Z)-2-methylbut-2-enedioic acid + NADPH
(2R)-2-methylbutanedioic acid + NADP+
Substrates: above 99% conversion with OPR-1
Products: -
Products: -
?
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
Substrates: enzyme is a FMN-dependent oxidoreductase that catalyzes the reduction of the cyclopentenone (9S,13S)-12-oxophytodienoate to the corresponding cyclopentanone in the biosynthesis of the plant hormone jasmonic acid
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(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+
?
Substrates: FMN-dependent oxidoreductase, biosynthesis of the plant hormone jasmonic acid, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae
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(9S,13S)-12-oxophytodienoate + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
Substrates: -
Products: -
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?
(9S,13S)-12-oxophytodienoic acid + NADPH + H+
3-oxo-2-((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
Substrates: OPR3 is involved in jasmonic acid biosynthesis
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?
1,4-benzoquinone + NADPH + H+
? + NADP+
Substrates: -
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?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
12-oxo-phytodienoic acid + NADPH
3-oxo-2-(2'(Z)-pentenyl)-cyclopentane-1 octanoic acid + NADP+
Substrates: enzyme catalyzes a late step in the jasmonic acid biosynthetic pathway
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12-oxo-trans-phythodienoic acid + alpha-NADPH
trans-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+
-
Substrates: -
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12-oxo-trans-phythodienoic acid + beta-NADPH
trans-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+
-
Substrates: -
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12-oxo-trans-phythodienoic acid + NADPH
t-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+
15-deoxy-DELTA12,14-prostaglandin J2 + alpha-NADPH
? + alpha-NADPH
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Substrates: PGJ2, an analogue of OPDA, rates of reaction with alpha-NADPH twenty times lower than those with beta-NADPH
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15-deoxy-DELTA12,14-prostaglandin J2 + beta-NADPH
? + NADP+
-
Substrates: PGJ2, an analogue of OPDA
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2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADH
(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NAD+
2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADPH
(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NADP+
2-methylcyclopent-2-en-1-one + NADPH + H+
(2S)-2-methylcyclopentanone + NAD+
Substrates: -
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3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADH
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADPH
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+
3-methylcyclopent-2-en-1-one + NADH
(3S)-3-methylcyclopentanone + NAD+
Substrates: 2% conversion with OPR-3
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3-methylcyclopent-2-en-1-one + NADPH
(3S)-3-methylcyclopentanone + NADP+
Substrates: 1% conversion with OPR-3
Products: -
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?
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
?
Substrates: in vitro, OPR3 reduces the jasmonic acid precursor (9S,13S)-12-oxophytodienoate as well as the enantiomeric (9R,13R)-12-oxophytodienoate, while its isozyme OPR1 is highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae
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8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
(15Z)-12-oxophyto-10,15-dienoate + NADPH + H+
-
Substrates: -
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?
9R,13R-12-oxo-phytodienoic acid + NADPH
? + NADP+
-
Substrates: oxylipin
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9S,13R-12-oxo-phytodienoic acid + NADPH
? + NADP+
-
Substrates: does not utilize 13S-configured isomers
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methyl vinyl ketone + NADPH + H+
hexanal + NADP+
Substrates: -
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?
N-ethyl-maleimide + NADPH + H+
? + NAD+
Substrates: -
Products: -
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?
trans-hex-2-enal + NADPH
hexanal + NADP+
Substrates: -
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?
trans-hex-2-enal + NADPH + H+
hexanal + NADP+
Substrates: -
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?
[(E)-1-methyl-2-nitroethenyl]benzene + NADH + H+
[(1R)-1-methyl-2-nitroethyl]benzene + NAD+
Substrates: above 99% conversion with OPR-1
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[(E)-1-methyl-2-nitroethenyl]benzene + NADH + H+
[(1S)-1-methyl-2-nitroethyl]benzene + NAD+
Substrates: 69% conversion with OPR-3
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?
[(E)-1-methyl-2-nitroethenyl]benzene + NADPH + H+
[(1R)-1-methyl-2-nitroethyl]benzene + NADP+
Substrates: above 99% conversion with OPR-1
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?
[(E)-1-methyl-2-nitroethenyl]benzene + NADPH + H+
[(1S)-1-methyl-2-nitroethyl]benzene + NADP+
Substrates: 72% conversion with OPR-3
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?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
Substrates: -
Products: -
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?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
Substrates: racemic mixture as substrate is accepted by isozyme OPR1
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?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: -
Products: -
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?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: leads to jasmonate biosynthesis
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?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: preference of (-)-cis isomer over the (+)-cis-isomer
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(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: -
Products: -
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?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
Substrates: -
Products: -
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?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
Substrates: -
Products: -
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?
(2E)-3,7-dimethylocta-2,6-dienal + NADH
(3S)-3,7-dimethyloct-6-enal + NAD+
Substrates: above 99% conversion with OPR-1
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?
(2E)-3,7-dimethylocta-2,6-dienal + NADH
(3S)-3,7-dimethyloct-6-enal + NAD+
Substrates: 90% conversion with OPR-3
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(2E)-3,7-dimethylocta-2,6-dienal + NADPH
(3S)-3,7-dimethyloct-6-enal + NADP+
Substrates: above 99% conversion with OPR-1
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(2E)-3,7-dimethylocta-2,6-dienal + NADPH
(3S)-3,7-dimethyloct-6-enal + NADP+
Substrates: 90% conversion with OPR-3
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(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: -
Products: -
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?
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: isozyme OPR1, no activity with the (9S,13S)-(15Z)-12-oxophyto-10,15-dienoate enantiomer
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(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: isozymes OPR3
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(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
Substrates: product is an intermediate in the jasmonate biosynthesis
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(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
Substrates: -
Products: -
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?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: product is the direct precursor in the jasmonate biosynthesis
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(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: isozyme OPR3
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(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
Substrates: -
Products: -
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(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
Substrates: OPR3 is the isoenzyme relevant for jasmonate biosynthesis
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(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
Substrates: OPRII reduces all 4 OPDA isomers
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(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
Substrates: reductase specific for the natural cis-OPDA enatiomer encoded by OPR2
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(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
Substrates: -
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: enzyme induced upon wounding
Products: OPC-8:0
Products: OPC-8:0
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: wound-induced gene activation
Products: OPC-8:0
Products: OPC-8:0
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: enzyme of the octadecanoid pathway leading to jasmonic acid
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: -
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
Substrates: -
Products: -
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: -
Products: -
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
Substrates: -
Products: -
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12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
Substrates: -
Products: -
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12-oxo-trans-phythodienoic acid + NADPH
t-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+
-
Substrates: -
Products: -
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12-oxo-trans-phythodienoic acid + NADPH
t-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+
-
Substrates: -
Products: -
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2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADH
(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NAD+
Substrates: above 98% conversion with OPR-1
Products: -
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2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADH
(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NAD+
Substrates: 77% conversion with OPR-3
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2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADPH
(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NADP+
Substrates: above 98% conversion with OPR-1
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2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADPH
(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NADP+
Substrates: 78% conversion with OPR-3
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2-cyclohexen-1-one + NADPH
? + NADP+
Substrates: model substrate, isozyme OPR4
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2-cyclohexen-1-one + NADPH
? + NADP+
Substrates: model substrate, isozyme OPR6
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Products: -
?
2-cyclohexen-1-one + NADPH
? + NADP+
Substrates: best substrate
Products: -
Products: -
?
2-cyclohexen-1-one + NADPH
? + NADP+
Substrates: model substrate, isozymes OPR1
Products: -
Products: -
?
2-cyclohexen-1-one + NADPH
? + NADP+
Substrates: model substrate, isozymes OPR2
Products: -
Products: -
?
2-cyclohexen-1-one + NADPH
? + NADP+
Substrates: model substrate, isozyme OPR3
Products: -
Products: -
?
2-methylcyclopent-2-en-1-one + NADH
(2S)-2-methylcyclopentanone + NAD+
Substrates: 58% conversion with OPR-1
Products: -
Products: -
?
2-methylcyclopent-2-en-1-one + NADH
(2S)-2-methylcyclopentanone + NAD+
Substrates: 19% conversion with OPR-3
Products: -
Products: -
?
2-methylcyclopent-2-en-1-one + NADPH
(2S)-2-methylcyclopentanone + NADP+
Substrates: 45% conversion with OPR-1
Products: -
Products: -
?
2-methylcyclopent-2-en-1-one + NADPH
(2S)-2-methylcyclopentanone + NADP+
Substrates: 27% conversion with OPR-3
Products: -
Products: -
?
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADH
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+
Substrates: 99% conversion with OPR-1
Products: -
Products: -
?
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADH
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+
Substrates: 99% conversion with OPR-3
Products: -
Products: -
?
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADPH
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+
Substrates: 99% conversion with OPR-1
Products: -
Products: -
?
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADPH
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+
Substrates: 99% conversion with OPR-3
Products: -
Products: -
?
beta-oestradiol + NADPH
? + NADP+
-
Substrates: very low but significant oxidoreductase activity, detected in micromolar quantities
Products: -
Products: -
?
additional information
?
-
-
Substrates: cyclohexanone, testosterone and progesterone are no substrates
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis, isozyme OPR3 is induced in self-defense signalling, enzyme is involved in octadecanoid and jasmonate biosynthesis with differential roles for in the local versus the systemic wound response, cDNA micro array analysis
Products: -
Products: -
?
additional information
?
-
-
Substrates: stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds
Products: -
Products: -
?
additional information
?
-
Substrates: OPR1 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives, it additionally produces the aromatic ring-reduced products hydride and dihydride Meisenheimer complexes
Products: -
Products: -
?
additional information
?
-
Substrates: OPR1 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives, it additionally produces the aromatic ring-reduced products hydride and dihydride Meisenheimer complexes
Products: -
Products: -
?
additional information
?
-
Substrates: OPR1 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives, it additionally produces the aromatic ring-reduced products hydride and dihydride Meisenheimer complexes
Products: -
Products: -
?
additional information
?
-
Substrates: OPR2 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives
Products: -
Products: -
?
additional information
?
-
Substrates: OPR2 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives
Products: -
Products: -
?
additional information
?
-
Substrates: OPR2 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives
Products: -
Products: -
?
additional information
?
-
Substrates: OPR3 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives
Products: -
Products: -
?
additional information
?
-
Substrates: OPR3 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives
Products: -
Products: -
?
additional information
?
-
Substrates: OPR3 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: suppressor-inducible enzyme activity leads to jasmonate biosynthesis or related compound, which might contribute to compatibility by specific activation of the octadecanoid pathway and inhibition of the phenylpropanoid biosynthetic pathway
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme OPR5 is inactive
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme OPR5 is inactive
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme OPR5 is inactive
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme OPR5 is inactive
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme OPR5 is inactive
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme OPR5 is inactive
Products: -
Products: -
?
additional information
?
-
Substrates: physiological role of the enzyme
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity towards fumaric acid, cyclohexanone and hexanal
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity towards fumaric acid, cyclohexanone, isomeride maleic acid and hexanal
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
Substrates: stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds
Products: -
Products: -
?
additional information
?
-
Substrates: stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds
Products: -
Products: -
?
additional information
?
-
Substrates: stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds
Products: -
Products: -
?
additional information
?
-
Substrates: no activity of isozyme OPR2 with (15Z)-12-oxophyto-10,15-dienoate stereoisomers
Products: -
Products: -
?
additional information
?
-
Substrates: no activity of isozyme OPR2 with (15Z)-12-oxophyto-10,15-dienoate stereoisomers
Products: -
Products: -
?
additional information
?
-
Substrates: no activity of isozyme OPR2 with (15Z)-12-oxophyto-10,15-dienoate stereoisomers
Products: -
Products: -
?
additional information
?
-
Substrates: stereospecificity of isozymes, overview, isozyme OPR3 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds
Products: -
Products: -
?
additional information
?
-
Substrates: stereospecificity of isozymes, overview, isozyme OPR3 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds
Products: -
Products: -
?
additional information
?
-
Substrates: stereospecificity of isozymes, overview, isozyme OPR3 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme OPR-1 does not convert 3-methylcyclopent-2-en-1-one
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme OPR-1 does not convert 3-methylcyclopent-2-en-1-one
Products: -
Products: -
?
additional information
?
-
-
Substrates: isozyme OPR-1 does not convert 3-methylcyclopent-2-en-1-one
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme OPR-3 does not convert (2Z)-2-methylbut-2-enedioic acid
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme OPR-3 does not convert (2Z)-2-methylbut-2-enedioic acid
Products: -
Products: -
?
additional information
?
-
-
Substrates: isozyme OPR-3 does not convert (2Z)-2-methylbut-2-enedioic acid
Products: -
Products: -
?
additional information
?
-
Substrates: OPR1-like enzymes are highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate
Products: -
Products: -
?
additional information
?
-
Substrates: OPR1-like enzymes are highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate
Products: -
Products: -
?
additional information
?
-
Substrates: the photoinduced electron transfer is compared in the structurally homologous enzymes 12-oxophytodienoate reductase 1 (OPR1) and Old Yellow Enzyme 1 (OYE1). The inside of a protein active site is a complex/heterogeneous dielectric network where genetically programmed heterogeneity near the site of biological electron transfer can significantly affect the presence and lifetime of various intermediate states
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: -
Products: -
Products: -
?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
Substrates: enzyme is a FMN-dependent oxidoreductase that catalyzes the reduction of the cyclopentenone (9S,13S)-12-oxophytodienoate to the corresponding cyclopentanone in the biosynthesis of the plant hormone jasmonic acid
Products: -
Products: -
?
(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+
?
Substrates: FMN-dependent oxidoreductase, biosynthesis of the plant hormone jasmonic acid, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae
Products: -
Products: -
?
(9S,13S)-12-oxophytodienoic acid + NADPH + H+
3-oxo-2-((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
Substrates: OPR3 is involved in jasmonic acid biosynthesis
Products: -
Products: -
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
12-oxo-phytodienoic acid + NADPH
3-oxo-2-(2'(Z)-pentenyl)-cyclopentane-1 octanoic acid + NADP+
Substrates: enzyme catalyzes a late step in the jasmonic acid biosynthetic pathway
Products: -
Products: -
?
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
?
Substrates: in vitro, OPR3 reduces the jasmonic acid precursor (9S,13S)-12-oxophytodienoate as well as the enantiomeric (9R,13R)-12-oxophytodienoate, while its isozyme OPR1 is highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae
Products: -
Products: -
?
9R,13R-12-oxo-phytodienoic acid + NADPH
? + NADP+
-
Substrates: oxylipin
Products: -
Products: -
?
9S,13R-12-oxo-phytodienoic acid + NADPH
? + NADP+
-
Substrates: does not utilize 13S-configured isomers
Products: -
Products: -
?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
Substrates: -
Products: -
Products: -
?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: leads to jasmonate biosynthesis
Products: -
Products: -
?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: -
Products: -
Products: -
?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
Substrates: product is an intermediate in the jasmonate biosynthesis
Products: -
Products: -
?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
Substrates: product is the direct precursor in the jasmonate biosynthesis
Products: -
Products: -
?
(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
Substrates: -
Products: -
Products: -
?
(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
Substrates: OPR3 is the isoenzyme relevant for jasmonate biosynthesis
Products: -
Products: -
?
(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
Substrates: OPRII reduces all 4 OPDA isomers
Products: -
Products: -
?
(9S,13S)-12-oxo-phytodienoic acid + NADPH
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
Substrates: reductase specific for the natural cis-OPDA enatiomer encoded by OPR2
Products: -
Products: -
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: enzyme induced upon wounding
Products: OPC-8:0
Products: OPC-8:0
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: wound-induced gene activation
Products: OPC-8:0
Products: OPC-8:0
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: enzyme of the octadecanoid pathway leading to jasmonic acid
Products: -
Products: -
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
Products: -
Products: -
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
Products: -
Products: -
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
Products: -
Products: -
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
Products: -
Products: -
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
Products: -
Products: -
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: -
Products: -
Products: -
?
12-oxo-cis-phythodienoic acid + NADPH
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
Substrates: third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA
Products: -
Products: -
?
additional information
?
-
-
Substrates: phylogenetic analysis, isozyme OPR3 is induced in self-defense signalling, enzyme is involved in octadecanoid and jasmonate biosynthesis with differential roles for in the local versus the systemic wound response, cDNA micro array analysis
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: suppressor-inducible enzyme activity leads to jasmonate biosynthesis or related compound, which might contribute to compatibility by specific activation of the octadecanoid pathway and inhibition of the phenylpropanoid biosynthetic pathway
Products: -
Products: -
?
additional information
?
-
Substrates: physiological role of the enzyme
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
Substrates: phylogenetic analysis
Products: -
Products: -
?
additional information
?
-
Substrates: OPR1-like enzymes are highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate
Products: -
Products: -
?
additional information
?
-
Substrates: OPR1-like enzymes are highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
flavin
-
dependent, contains one molar equivalent of noncovalently bound FMN per enzyme subunit
additional information
in the dimer interface of enzyme, a sulfate ion is bound which forms hydrogen bonds with two arginines of protomer A and one arginine of loop L6 of the partner protomer B. Sulfate ion may mimic the phosphate group of phosphorylated T364
-
additional information
-
in the dimer interface of enzyme, a sulfate ion is bound which forms hydrogen bonds with two arginines of protomer A and one arginine of loop L6 of the partner protomer B. Sulfate ion may mimic the phosphate group of phosphorylated T364
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cis-3-hexen-1-yl acetate
OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants
2,4,6-trinitrotoluene
expression of oxophytodienoate reductases increases in response to the explosive 2,4,6-trinitrotoluene, 17fold up-regulation of OPR1 in the root
2,4,6-trinitrotoluene
expression of oxophytodienoate reductases increases in response to the explosive 2,4,6-trinitrotoluene, 6fold up-regulation of OPR2 in the root
additional information
enzyme expression is inducible by regulatory suppressor glycopeptides from the fungus Mycosphaerella pinodes, which block the pea defense response induced by elicitors
-
additional information
isozyme OPR2, i.e. S64, expression is induced by substrate, methyljasmonate, or compatible pathogens, or a suppressor derived from fungus Mycosphaerella pinodes, isozyme OPR2 is induced by coronatine
-
additional information
isozyme OPR2, i.e. S64, expression is induced by substrate, methyljasmonate, or compatible pathogens, or a suppressor derived from fungus Mycosphaerella pinodes, isozyme OPR2 is induced by coronatine
-
additional information
isozyme OPR2, i.e. S64, expression is induced by substrate, methyljasmonate, or compatible pathogens, or a suppressor derived from fungus Mycosphaerella pinodes, isozyme OPR2 is induced by coronatine
-
additional information
isozyme OPR2, i.e. S64, expression is induced by substrate, methyljasmonate, or compatible pathogens, or a suppressor derived from fungus Mycosphaerella pinodes, isozyme OPR2 is induced by coronatine
-
additional information
isozyme OPR2, i.e. S64, expression is induced by substrate, methyljasmonate, or compatible pathogens, or a suppressor derived from fungus Mycosphaerella pinodes, isozyme OPR2 is induced by coronatine
-
additional information
isozyme OPR2, i.e. S64, expression is induced by substrate, methyljasmonate, or compatible pathogens, or a suppressor derived from fungus Mycosphaerella pinodes, isozyme OPR2 is induced by coronatine
-
additional information
isozymes OPR3 is slightly induced by coronatine
-
additional information
isozymes OPR3 is slightly induced by coronatine
-
additional information
isozymes OPR3 is slightly induced by coronatine
-
additional information
isozymes OPR3 is slightly induced by coronatine
-
additional information
isozymes OPR3 is slightly induced by coronatine
-
additional information
isozymes OPR3 is slightly induced by coronatine
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Dehydration
Induction of the Arabidopsis PHO1;H10 gene by 12-oxo-phytodienoic acid but not jasmonic acid via a CORONATINE INSENSITIVE1-dependent pathway.
Infertility, Male
Creation of male-sterile lines that can be restored to fertility by exogenous methyl jasmonate for the establishment of a two-line system for the hybrid production of rice (Oryza sativa L.).
Infertility, Male
Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.967
2-methylcyclopent-2-en-1-one
pH 7.2, temperature not specified in the publication
0.0385
Methyl vinyl ketone
pH 7.2, temperature not specified in the publication
4.36
trans-hex-2-enal
pH 7.2, temperature not specified in the publication
0.0283
(15Z)-12-oxophyto-10,15-dienoate
pH 7.2, temperature not specified in the publication
0.043
(15Z)-12-oxophyto-10,15-dienoate
pH 7.5, 25°C, recombinant enzyme
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
for oxidative half-reaction, kox of trans-hex-2-2enal is 0.3 per sec
-
additional information
additional information
-
for oxidative half-reaction, kox of trans-hex-2-2enal is 0.3 per sec
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.6
13.5
substrate methyl vinyl ketone, pH 7.2, temperature not specified in the publication
2
substrate (15Z)-12-oxophyto-10,15-dienoate, pH 7.2, temperature not specified in the publication
2.5
substrate 2-methylcyclopent-2-en-1-one, pH 7.2, temperature not specified in the publication
22.8
substrate N-ethyl-maleimide, pH 7.2, temperature not specified in the publication
26.9
substrate 1,4-benzoquinone, pH 7.2, temperature not specified in the publication
additional information
0.6
substrate maleic acid, pH 7.2, temperature not specified in the publication
0.6
substrate trans-hex-2-enal, pH 7.2, temperature not specified in the publication
additional information
OPR1-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco, OPR1-overexpressing lines 1.1 and 9.4 show enhanced 2,4,6-trinitrotoluene uptake
additional information
OPR1-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco, OPR1-overexpressing lines 1.1 and 9.4 show enhanced 2,4,6-trinitrotoluene uptake
additional information
OPR1-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco, OPR1-overexpressing lines 1.1 and 9.4 show enhanced 2,4,6-trinitrotoluene uptake
additional information
OPR2-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco
additional information
OPR2-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco
additional information
OPR2-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco
additional information
-
jasmonic acid deficiency in OPR-interfering RNA plants does not influence resistance to Magnaporthe grisea or the pathogenesis-related gene expressions in these plants
additional information
jasmonic acid deficiency in OPR-interfering RNA plants does not influence resistance to Magnaporthe grisea or the pathogenesis-related gene expressions in these plants
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isozyme OPR1; 6 isozymes of enzyme family subgroup I
SwissProt
brenda
isozyme OPR2; 6 isozymes of enzyme family subgroup I
SwissProt
brenda
isozyme OPR3; 6 isozymes of enzyme family subgroup I
SwissProt
brenda
isozyme OPR4; 6 isozymes of enzyme family subgroup I
SwissProt
brenda
isozyme OPR5; 6 isozymes of enzyme family subgroup I
SwissProt
brenda
isozyme OPR6; 6 isozymes of enzyme family subgroup I
SwissProt
brenda
isozyme OPR2, 12-oxophytodienoate-like protein; 3 isozymes OPR1-3, 12-oxophytodienoate-like protein
SwissProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
-
isozyme OPR3, up-regulation in wounded leaves, low activity in unwounded, systemic leaves
brenda
-
HvOPR1 expression is developmentally regulated during primary leaf development
brenda
additional information
-
CsOPR3 is highly expressed in flowers, leaves and stems but is weakly expressed in roots and seeds, quantitative real-time PCR tissue expression analysis
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
-
the enzyme lacks the OPRII-specific peroximal signal peptide at its C terminus
-
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
metabolism
physiological function
evolution
-
in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview
evolution
-
in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview
evolution
in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview
evolution
-
in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview
evolution
-
in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview
evolution
-
in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview
evolution
-
in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview
evolution
-
in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview
evolution
-
in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview
evolution
-
in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview
evolution
-
in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview
evolution
-
the TaOPR1 sequence retains the conserved residues that account for the NADPH- and FMN-binding sites of the OYE family
metabolism
-
enzyme TaOPR1 stimulated the abscisic acid-dependent stress-responsive pathway
metabolism
one of the key genes of the jasmonic acid biosynthesis pathway
physiological function
-
12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid
physiological function
-
12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid
physiological function
12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid
physiological function
-
12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid
physiological function
-
12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid
physiological function
-
12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid
physiological function
-
12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid
physiological function
-
12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid
physiological function
-
12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid
physiological function
-
12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid
physiological function
-
12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid
physiological function
-
12-oxophytodienoate reductase (OPR) is a key enzyme in the biosynthesis of jasmonic acid (JA), which plays an important role in plant defense responses. Enzyme CsOPR3 plays an important role in jasmonate biosynthesis and tea plant defense against herbivorous insects
physiological function
-
enzyme TaOPR1 does not interact with jasmonate synthesis or the jasmonate signaling pathway. Rather than serving purely as an antioxidant, TaOPR1 acts during episodes of abiotic stress response as a signaling compound associated with the regulation of the abscisic acid-mediated signaling network. Overexpression of TaOPR1 in wheat significantly enhances the level of salinity tolerance, while its heterologous expression in Arabidopsis thaliana alleviates root growth restriction in the presence of salinity and oxidants and raises the sensitivity to abscisic acid. In Arabidopsis, recombinant TaOPR1 promotes abscisic acid synthesis. and the abscisic acid-dependent stress-responsive pathway. Recombinant TaOPR1 partially rescues the sensitivity of the Arabidopsis thaliana aba2 mutant defective in abscisic acid synthesis to salinity, and improves the activities of reactive oxygen species scavengers and the transcription of their encoding genes while reducing malondialdehyde and reactive oxygen species levels. TaOPR1 enhanced the salinity tolerance of wheat and in transgenic Arabidopsis thaliana. TaOPR1-overexpressing lines show insensitivity to H2O2
physiological function
transgenic wheat plants with high AtOPR3 expression levels are characterized by delayed germination, slower growth, late flowering and senescence, and improved tolerance to short-term freezing
physiological function
OPR3 is a bifunctional enzyme with NADPH-dependent alpha,beta-ketoalkene double-bond reductase and monodehydroascorbate reductase activities. OPR3 mutants show a slightly less-reduced ascorbate pool in leaves in line with the MDHAR activity of OPR3 in vitro. OPR3 catalyzes the reduction of a wide spectrum of electrophilic species that share a reactivity toward the glutathione and ascorbate. 12-oxophytodienoic acid reacts with ascorbate to form an ascorbate-12-oxophytodienoic acid adduct, but in addition OPR3 has the ability to regenerate ascorbate from monodehydroascorbate
Show AA Sequence and Transmembrane Helices (334 entries)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
monomer
additional information
dimer
concentration-dependent shift from monomer with 40000 Da at 0.022 mM to dimer with 80000 Da at 0.358 mM, dynamic light scattering
monomer
concentration-dependent shift from monomer with 40000 Da at 0.022 mM to dimer with 80000 Da at 0.358 mM, dynamic light scattering
additional information
rapid monomer-dimer equilibrium with a high dissociation constant in vitro
additional information
-
rapid monomer-dimer equilibrium with a high dissociation constant in vitro
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
10 mg/ml purified recombinant isozyme OPR3 in 100 mM MES, pH 6.0, 100 mM NaCl, 0.3 mM TCEP, hanging drop vapour diffusion method, 0.002 ml protein solution with equal volume of well solution containing 8.75-10.0% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.275-0.35 M glycine, crystals are harvested into a cryoprotectant solution containing 12% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.4 M glycine, and 25% PEG 400, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement
-
crystal structure of Arabidopsis thaliana OPR3 in complex with 8-iso prostaglandin A1 (8-iso PGA1) is reported. The crystal structure is solved to 2.6 A. The positioning of 8-iso PGA1 reveals a new binding orientation for substrate in the active site that likely contributes to the relaxed stereospecificity observed for AtOPR3 relative to other OPR's
-
one enzyme monomer per asymmetric unit, space group C2221. Enzyme is a member of an alpha/beta barrel fold family of FMN-containing oxidoreductases
-
at 2.0 A resolution, determination of the crystal structures of OPR3 in complex with the ligand p-hydroxybenzaldehyde, structural comparison with the OPR1:(9R,13R)-12-oxophytodienoate complex and further biochemical and mutational analyses reveals that 2 active-site residues, Phe74 and His244 in OPR3 are critical for substrate filtering
native enzyme and mutants E291L and Y364F. Wild-type enzyme crystallizes as an extraordinary self-inhibiting dimer, dimerization is actively driven by the mutual binding of the two L6 loops into the two active sites
OPR1:p-hydroxybenzaldehyde complex at 2.3 A resolution, determination of the crystal structures of OPR1 in complex with the ligand p-hydroxybenzaldehyde, structural comparison with the OPR1:(9R,13R)-12-oxophytodienoate complex and further biochemical and mutational analyses reveals that 2 active-site residues, Tyr78 and Tyr246 in OPR1 are critical for substrate filtering
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
E291L
sixfold faster turnover than wild-type. Crystallization in same space group as wild-type, but with strikingly different cell constants. Appears as monomer in the crystal
F74Y
OPR3 mutant, change in substrate specificity, similar increase in stereoselectivity is observed for the mutant as compared to the wild-type enzyme
F74Y/H244Y
OPR3 double-mutant, reduction of (9S,13S)-12-oxo-phytodienoic acid is slower in the double-mutant as in the wild type enzyme and the single mutants, the protein crystallizes as a monomer with none of the dimer interactions retain
H244Y
OPR3 mutant, change in substrate specificity, similar increase in stereoselectivity is observed for the mutant as compared to the wild-type enzyme
Y364F
crystallization as a monomer with none of the dimer interactions retained
additional information
additional information
-
construction of isozyme OPR3 mutant plants for subcellular localization study of the isozyme, overview
additional information
-
overexpression of TaOPR1 in wheat significantly enhances the level of salinity tolerance, while its heterologous expression in Arabidopsis thaliana alleviates root growth restriction in the presence of salinity and oxidants and raises the sensitivity to abscisic acid. In Arabidopsis, recombinant TaOPR1 promotes abscisic acid synthesis. and the abscisic acid-dependent stress-responsive pathway. Recombinant TaOPR1 partially rescues the sensitivity of the Arabidopsis thaliana aba2 mutant defective in abscisic acid synthesis to salinity, and improves the activities of reactive oxygen species scavengers and the transcription of their encoding genes while reducing malondialdehyde and reactive oxygen species levels. After exposure to 200 mM NaCl, the wild-type seedlings become very wilted while the TaOE ones remain fully turgid. The TaOE line shoots and roots are longer than those of the wild type, phenotype, overview. Inconsistent with the positive effect of TaOPR1, the double RNA interference (RNAi) knockdown mutant opr1opr2, from two homologous OPRI genes in Arabidopsis thaliana, do not increase the sensitivity to NaCl treatment, and their phenotypes are comparable to the wild-type. TaOPR1-overexpressing lines show insensitivity to H2O2
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant His-tagged isozyme OPR1 from Escherichia coli by nickel affinity chromatography
recombinant His-tagged isozyme OPR2 from Escherichia coli by nickel affinity chromatography
recombinant His-tagged isozyme OPR3 from Escherichia coli by nickel affinity chromatography
recombinant isozyme from Escherichia coli
recombinant isozyme OPR3 from Escherichia coli by nickel-imino-diacetic acid affinity chromatography
-
recombinant tagged isozymes OPR1-3 from Escherichia coli by affinity chromatography
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA library construction, protein S64, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as His-tagged protein
cloning, and DNA sequence determination and analysis, expression of gene At2g06050, encoding isozyme OPR3, in Escherichia coli as N-terminally fusion protein fused to S-tag-His6-maltose binding protein
-
construction of a cDNA library, phylogenetic analysis, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3)
construction of a genomic DNA library, identification and DNA sequence comparison of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3)
construction of a genomic DNA library, identification of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3)
enzyme cloned from its cDNA, open reading frame encodes a 372 amino acids polypeptide, functionally expressed in Escherichia coli
expressed in Arabidopsis thaliana through transformation with Agrobacterium tumefaciens strain EHA105
-
expressed in Arabidopsis thaliana through transformation with Agrobacterium tumefaciens strain GV3101
expressed in Escherichia coli
expression of His-tagged isozyme OPR1 in Escherichia coli, transient expression of isozyme OPR1 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study
expression of His-tagged isozyme OPR2 in Escherichia coli
expression of His-tagged isozyme OPR3 in Escherichia coli, transient expression of isozyme OPR3 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study
expression of the 3 isozymes OPR1-3 as N-terminally GST- or His-tagged enzymes in Escherichia coli
-
gene CsOPR3, a putative OPR gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis
-
gene TaOPR1, TaOPR1 maps to the short arm of the second chromosome of the B genome (2BS), DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression in Arabidopsis thaliana ecotype Col-0. The repression of AtOPR1 by the TaOPR1 transgene suggests a negative feedback regulatory effect, perhaps based on functional redundancy between TaOPR1 and AtOPR1
-
generating of a jasmonic acid-deficient rice line with suppressed expression of the gene encoding OPR3 with resistance to Magnaporthe grisea by introduction into rice plants by Agrobacterium-mediated transformation, OPR-interfering RNA plants show normal resistance to both an incompatible and a compatible race of Magnaporthe grisea
generating of a jasmonic acid-deficient rice line with suppressed expression of the genes encoding OPR1 with resistance to Magnaporthe grisea by introduction into rice plants by Agrobacterium-mediated transformation, OPR-interfering RNA plants show normal resistance to both an incompatible and a compatible race of Magnaporthe grisea
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
OPR1 and OPR2, two closely related genes encodes 12-oxophytodienoic acid-10,11-reductases
-
OPR3 expressed in functional form in Escherichia coli, cloning of OPR2 cDNA by reverse transcriptase-polymerase chain reaction, OPR2 expressed in insect cell cultures of Spodoptera frugiperda with transgenic Autographa californica baculoviruses and overexpressed in transgenic Arabidopsis thaliana
-
overexpression of Arabidopsis OPR3 in hexaploid wheat (Triticum aestivum) alters plant development and freezing tolerance. Transgenic wheat plants with high AtOPR3 expression levels are characterized by delayed germination, slower growth, late flowering and senescence, and improved tolerance to short-term freezing
recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR1
recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR2
recombinant expression of OPR3 in Escherichia coli
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
-
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
-
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
-
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
-
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
-
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
-
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
-
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
-
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
-
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
CsOPR3 expression is rapidly induced by mechanical wounding, increased jasmonic acid levels are correlated with the wound-induced CsOPR3 expression. Infection with tea plant pest Ectropis obliqua also induces the enzyme expression. Exogenous jasmonic acid induces the enzyme expression, and induced enzyme expression increases the levels of jasmonate, overview
-
salinity stress induces a higher level of TaOPR1 expression in the seedling roots of cv. SR3 compared to parental cultivar JN177. This induction is abolished when abscisic acid (ABA) synthesis is inhibited
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up-regulated expression in response to drought, hydrogen peroxide and wounding. Accumulation of HvOPR1 mRNA is increased in response to jasmonic acid, salicylic acid and abscisic acid
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with prolonged cultivation time of grey poplar genotypes, the relative expression patterns of OPR1 increases in root and in shoot. Significantly higher OPR1 transcript levels are detected in the roots of phenotype TP11 and phenotype TP20 after 10 days of Cd treatment, especially in phenotyp TP11 (38.4fold) grown under 0.01 mM Cd, compared to the control. As in the roots, the level of transcription in the leaves is also upregulated after 10 days of Cd treatment. The highest upregulation of ORP1 (16.4fold) is measured in the leaves of phenotype TP20 cultivated with 0.01 mM Cd
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
environmental protection
2,4,6-trinitrotoluene detoxofication, use of plants to remove environmental pollutants
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REF.
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TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
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Arabidopsis thaliana, Capnoides sempervirens, Saccharomyces cerevisiae
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Solanum lycopersicum
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Lathyrus oleraceus (Q9AVK9)
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Solanum lycopersicum (Q9XG54), Solanum lycopersicum (Q9FEX0), Solanum lycopersicum (Q9FEW9), Arabidopsis thaliana
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Cloning and characterization of a jasmonic acid-responsive gene encoding 12-oxophytodienoic acid reductase in suspension-cultured rice cells
Planta
216
692-698
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Oryza sativa (Q84QK0)
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Malone, T.E.; Madson, S.E.; Wrobel, R.L.; Jeon, W.B.; Rosenberg, N.S.; Johnson, K.A.; Bingman, C.A.; Smith, D.W.; Phillips, G.N., Jr.; Markley, J.L.; Fox, B.G.
X-ray structure of Arabidopsis At2g06050, 12-oxophytodienoate reductase isoform 3
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58
243-245
2004
Arabidopsis thaliana
brenda
Breithaupt, C.; Kurzbauer, R.; Lilie, H.; Schaller, A.; Strassner, J.; Huber, R.; Macheroux, P.; Clausen, T.
Crystal structure of 12-oxophytodienoate reductase 3 from tomato: self-inhibition by dimerization
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Solanum lycopersicum (Q9FEW9), Solanum lycopersicum
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X-ray structure of Arabidopsis At1g77680, 12-oxophytodienoate reductase isoform 1
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61
206-208
2005
Arabidopsis thaliana
brenda
Craig, D.H.; Moody, P.C.; Bruce, N.C.; Scrutton, N.S.
Reductive and oxidative half-reactions of morphinone reductase from Pseudomonas putida M10: a kinetic and thermodynamic analysis
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37
7598-7607
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Pseudomonas putida
brenda
Hall, M.; Stueckler, C.; Kroutil, W.; Macheroux, P.; Faber, K.
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Solanum lycopersicum (Q9XG54), Solanum lycopersicum (Q9FEW9), Solanum lycopersicum
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2007
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Zea mays (Q49HE4)
-
brenda
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Planta
227
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Oryza sativa
brenda
Yara, A.; Yaeno, T.; Hasegawa, M.; Seto, H.; Seo, S.; Kusumi, K.; Iba, K.
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Solanum lycopersicum (Q9XG54), Solanum lycopersicum (Q9FEW9)
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Arabidopsis thaliana (Q8LAH7), Arabidopsis thaliana (Q8GYB8), Arabidopsis thaliana (Q9FUP0)
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Hordeum vulgare
-
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Oryza sativa
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Crystal structure of Arabidopsis thaliana 12-oxophytodienoate reductase isoform 3 in complex with 8-iso prostaglandin A 1
Proteins
79
3236-3241
2011
Arabidopsis thaliana
brenda
Xin, Z.; Zhang, J.; Ge, L.; Lei, S.; Han, J.; Zhang, X.; Li, X.; Sun, X.
A putative 12-oxophytodienoate reductase gene CsOPR3 from Camellia sinensis, is involved in wound and herbivore infestation responses
Gene
615
18-24
2017
Camellia sinensis
brenda
Dong, W.; Wang, M.; Xu, F.; Quan, T.; Peng, K.; Xiao, L.; Xia, G.
Wheat oxophytodienoate reductase gene TaOPR1 confers salinity tolerance via enhancement of abscisic acid signaling and reactive oxygen species scavenging
Plant Physiol.
161
1217-1228
2013
Triticum aestivum
brenda
Pigolev, A.V.; Miroshnichenko, D.N.; Pushin, A.S.; Terentyev, V.V.; Boutanayev, A.M.; Dolgov, S.V.; Savchenko, T.V.
Overexpression of Arabidopsis OPR3 in hexaploid wheat (Triticum aestivum L.) alters plant development and freezing tolerance
Int. J. Mol. Sci.
19
3989
2018
Arabidopsis thaliana (Q9FUP0)
brenda
Kudisch, B.; Oblinsky, D.G.; Black, M.J.; Zieleniewska, A.; Emmanuel, M.A.; Rumbles, G.; Hyster, T.K.; Scholes, G.D.
Active-site environmental factors customize the photophysics of photoenzymatic old yellow enzymes
J. Phys. Chem. B
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11236-11249
2020
Solanum lycopersicum (Q9XG54)
brenda
Komarkova, M.; Chromy, J.; Pokorna, E.; Soudek, P.; Machova, P.
Physiological and transcriptomic response of grey poplar (Populus x canescens Aiton Sm.) to cadmium stress
Plants (Basel)
9
1485
2020
Populus tremula x Populus alba
brenda
Maynard, D.; Kumar, V.; Sproi, J.; Dietz, K.J.
12-Oxophytodienoic acid reductase 3 (OPR3) functions as NADPH-dependent alpha,beta-ketoalkene reductase in detoxification and monodehydroascorbate reductase in redox homeostasis
Plant Cell Physiol.
61
584-595
2020
Arabidopsis thaliana (Q9FUP0)
brenda
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