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IUBMB Comments Binds FMN and FAD . Other class 1 dihydroorotate dehydrogenases use either fumarate (EC 1.3.98.1 ) or NAD+ EC 1.3.1.14 ) as electron acceptor. The membrane bound class 2 dihydroorotate dehydrogenase (EC 1.3.5.2 ) uses quinone as electron acceptor .
Synonyms
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reductase, orotate (reduced nicotinamide adenine dinucleotide phosphate)
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triphosphopyridine-linked dihydroorotic dehydrogenase
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(S)-dihydroorotate + NADP+ = orotate + NADPH + H+
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(S)-dihydroorotate:NADP+ oxidoreductase
Binds FMN and FAD [2]. Other class 1 dihydroorotate dehydrogenases use either fumarate (EC 1.3.98.1) or NAD+ (EC 1.3.1.14) as electron acceptor. The membrane bound class 2 dihydroorotate dehydrogenase (EC 1.3.5.2) uses quinone as electron acceptor .
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5-fluoroorotate + NADPH
(S)-5-fluorodihydroorotate + NADP+
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Substrates: enzyme exhibits very weak oxidase activity
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orotate + NADPH
(S)-dihydroorotate + NADP+
orotate + NADPH
(S)-dihydroorotate + NADP+
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Substrates: -
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orotate + NADPH
(S)-dihydroorotate + NADP+
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Substrates: enzyme is 20fold less active with NADH as cofactor
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orotate + NADPH
(S)-dihydroorotate + NADP+
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Substrates: degradative enzyme
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orotate + NADPH
(S)-dihydroorotate + NADP+
orotate + NADPH
(S)-dihydroorotate + NADP+
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Substrates: -
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orotate + NADPH
(S)-dihydroorotate + NADP+
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Substrates: degradative enzyme
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FAD
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ratio FAD/FMN 1:1 (probably)
FMN
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ratio FAD/FMN 1:1 (probably)
NADPH
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additional information
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Mg2+ has no effect
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2,4-Dihydroxy-6-methylpyridine
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7.8 mM, 22% inhibition
5-Methylorotic acid
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6 mM, 63% inhibition
Barbituric acid
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2 mM, 60% inhibition
p-chloromercuribenzoate
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0.01 mM, 90% inhibition, 0.001 mM, 30% inhibition
Uracil-5-carboxylic acid
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7.8 mM, 30% inhibition
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0.19
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enzyme activity in cells grown on orotate
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7.3
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sodium phosphate buffer
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6.7 - 8.8
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at pH 6.7 and 8.8: about 25% of activity maximum
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gram-negative, non-spore forming obligate aerobe
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brenda
pseudomonad isolated from lake bank mud, capable of growing on salt medium with glucose, aspartate, glycerol or orotate as carbon source
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brenda
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brenda
Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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A0A485M592_9ZZZZ
316
0
33212
TrEMBL
other Location (Reliability: 2 )
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60
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8 min, 81% loss of activity
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-20°C, 4 months, complete loss of activity
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Taylor, W.H.; Taylor, M.L.; Eames, D.F.
Two functionally different dihydroorotic dehydrogenases in bacteria
J. Bacteriol.
91
2251-2256
1966
Pseudomonas sp.
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Udaka, S.; Vennesland, B.
Properties of triphosphpyridine nucleotide-linked dihydroorotic dehydrogenase
J. Biol. Chem.
237
2018-2024
1962
Bacteria
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