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IUBMB Comments Binds FAD covalently. Part of the cannabinoids biosynthetic pathway of the plant Cannabis sativa . The enzyme can also convert cannabinerolate to cannabidiolate with lower efficiency.
Synonyms
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cannabidiolic-acid synthase
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CBDA synthase
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cannabigerolate + O2 = cannabidiolate + H2O2
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MetaCyc
cannabinoid biosynthesis
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cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming)
Binds FAD covalently. Part of the cannabinoids biosynthetic pathway of the plant Cannabis sativa. The enzyme can also convert cannabinerolate to cannabidiolate with lower efficiency.
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cannabigerolate + O2
cannabidiolate + H2O2
cannabinerolate + O2
cannabidiolate + H2O2
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Substrates: -
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additional information
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Substrates: the enzyme does not require molecular oxygen for the production of cannabidiolate in the presence or absence of NADPH
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cannabigerolate + O2
cannabidiolate + H2O2
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Substrates: -
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cannabigerolate + O2
cannabidiolate + H2O2
Substrates: -
ir
cannabigerolate + O2
cannabidiolate + H2O2
Substrates: -
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cannabigerolate + O2
cannabidiolate + H2O2
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Substrates: best substrate
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cannabigerolate + O2
cannabidiolate + H2O2
cannabigerolate + O2
cannabidiolate + H2O2
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Substrates: -
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cannabigerolate + O2
cannabidiolate + H2O2
Substrates: -
?
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FAD
the enzyme has a covalently bound flavin
additional information
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the enzyme does not require any coenzyme like NAD+, NADP+, FAD, and FMN
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additional information
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the enzyme does not require metal ions
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Hg2+
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complete inhibition at 2 mM
additional information
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EDTA has little influence on the enzyme activity at concentrations up to 5 mM
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Prostatic Neoplasms
Development and initial psychometric evaluation of the computer-based prostate Cancer screening decision aid acceptance scale for African-American men.
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0.137
cannabigerolate
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in 100 mM sodium citrate buffer (pH 5.0), at 30°C
0.206
cannabinerolate
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in 100 mM sodium citrate buffer (pH 5.0), at 30°C
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0.19
cannabigerolate
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in 100 mM sodium citrate buffer (pH 5.0), at 30°C
0.03
cannabinerolate
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in 100 mM sodium citrate buffer (pH 5.0), at 30°C
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0.175
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enzyme from supernatant, in 100 mM sodium citrate buffer (pH 5.0), at 30°C
9
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after 519fold purification, in 100 mM sodium citrate buffer (pH 5.0), at 30°C
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4 - 6
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half-maximal activities at pH values around 4.0 and 6.0
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6.1
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isoelectric focusing
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UniProt
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CBDA and Mexican strains
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brenda
CBDA strain
UniProt
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cultivars Skunk #1 and Carmen
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brenda
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brenda
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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CBDAS_CANSA
544
1
62237
Swiss-Prot
Secretory Pathway (Reliability: 1 )
A0A2P6Q6L3_ROSCH
125
0
14872
TrEMBL
other Location (Reliability: 2 )
A0A2P6R0P3_ROSCH
92
0
10673
TrEMBL
other Location (Reliability: 2 )
A0A084G6X6_PSEDA
125
0
13506
TrEMBL
other Location (Reliability: 2 )
A0A2P6Q6N0_ROSCH
189
2
21163
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A0E3XIC7_CANSA
544
0
62168
TrEMBL
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58863
x * 58863, deduced from amino acid sequence
62000
x * 62000, SDS-PAGE
74000
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1 * 74000, SDS-PAGE
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monomer
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1 * 74000, SDS-PAGE
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x * 62000, SDS-PAGE
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x * 58863, deduced from amino acid sequence
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-20°C, 40% (v/v) glycerol, ineffective storage conditions for enzyme stabilization
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4°C, purified enzyme in 10 mM sodium phosphate buffer (pH 7.0), 3 mM beta-mercaptoethanol, 2 weeks, 10-20% loss of activity
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ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose CL-4B column chromatography, and hydroxylapatite column chromatography
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hydroxylapatite column chromatography
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expressed in Sf9 insect cells
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Taura, F.; Sirikantaramas, S.; Shoyama, Y.; Yoshikai, K.; Shoyama, Y.; Morimoto, S.
Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa
FEBS Lett.
581
2929-2934
2007
Cannabis sativa (A6P6V9), Cannabis sativa
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Taura, F.; Morimoto, S.; Shoyama, Y.
Purification and characterization of cannabidiolic-acid synthase from Cannabis sativa L.. Biochemical analysis of a novel enzyme that catalyzes the oxidocyclization of cannabigerolic acid to cannabidiolic acid
J. Biol. Chem.
271
17411-17416
1996
Cannabis sativa
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Weiblen, G.D.; Wenger, J.P.; Craft, K.J.; ElSohly, M.A.; Mehmedic, Z.; Treiber, E.L.; Marks, M.D.
Gene duplication and divergence affecting drug content in Cannabis sativa
New Phytol.
208
1241-1250
2015
Cannabis sativa
brenda
Onofri, C.; de Meijer, E.P.M.; Mandolino, G.
Sequence heterogeneity of cannabidiolic- and tetrahydrocannabinolic acid-synthase in Cannabis sativa L. and its relationship with chemical phenotype
Phytochemistry
116
57-68
2015
Cannabis sativa (A0A0E3XIC7), Cannabis sativa
brenda
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