We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 1.2.1.85 - 2-hydroxymuconate-6-semialdehyde dehydrogenase for references in articles please use BRENDA:EC1.2.1.85
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments This substrate for this enzyme is formed by meta ring cleavage of catechol (EC 1.13.11.2, catechol 2,3-dioxygenase), and is an intermediate in the bacterial degradation of several aromatic compounds. Has lower activity with benzaldehyde . Activity with NAD+ is more than 10-fold higher than with NADP+ . cf. EC 1.2.1.32, aminomuconate-semialdehyde dehydrogenase.
The enzyme appears in viruses and cellular organisms
Synonyms
2-hydroxymuconate semialdehyde dehydrogenase, 2-hydroxymuconic semialdehyde dehydrogenase, 5C-2HMS dehydrogenase, HMS dehydrogenase, NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase, NahI, praB, XylG,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-hydroxymuconate semialdehyde dehydrogenase
2-hydroxymuconic semialdehyde dehydrogenase
NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase
2-hydroxymuconate semialdehyde dehydrogenase
Q1XGK8
-
2-hydroxymuconate semialdehyde dehydrogenase
Q1XGK8
-
-
2-hydroxymuconic semialdehyde dehydrogenase
-
-
2-hydroxymuconic semialdehyde dehydrogenase
-
-
-
5C-2HMS dehydrogenase
-
-
5C-2HMS dehydrogenase
-
-
-
HMS dehydrogenase
-
-
NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase
-
-
NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase
-
-
-
NahI
Q1XGK8
; gene name
NahI
Q1XGK8
; gene name
-
praB
-
-
-
-
XylG
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O = (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-hydroxymuconate-6-semialdehyde:NAD+ oxidoreductase
This substrate for this enzyme is formed by meta ring cleavage of catechol (EC 1.13.11.2, catechol 2,3-dioxygenase), and is an intermediate in the bacterial degradation of several aromatic compounds. Has lower activity with benzaldehyde [1]. Activity with NAD+ is more than 10-fold higher than with NADP+ [3]. cf. EC 1.2.1.32, aminomuconate-semialdehyde dehydrogenase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O
(2E,4Z)-2-hydroxy-5-methylhexa-2,4-diendioate + NADH + H+
-
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
3-chlorobenzaldehyde + NAD+ + H2O
3-chlorobenzoate + NADH + H+
-
-
-
-
?
3-fluorobenzaldehyde + NAD+ + H2O
3-fluorobenzoate + NADH + H+
-
-
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
3-methoxybenzoate + NADH + H+
-
-
-
-
?
3-methylbenzaldehyde + NAD+ + H2O
3-methylbenzoate + NADH + H+
-
-
-
-
?
3-nitrobenzaldehyde + NAD+ + H2O
3-nitrobenzoate + NADH + H+
-
-
-
-
?
4-methylbenzaldehyde + NAD+ + H2O
4-methylbenzoate + NADH + H+
-
-
-
-
?
5-chloro-2-hydroxymuconic semialdehyde + NAD+ + H2O
5-chloro-2-hydroxymuconic acid + NADH + H+
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
-
113% activity compared to 2-hydroxymuconate-6-semialdehyde
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
butyraldehyde + NAD+ + H2O
butanoate + NADH + H+
-
138% activity compared to 2-hydroxymuconate-6-semialdehyde
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH + H+
-
16.5% activity compared to 2-hydroxymuconate-6-semialdehyde
-
-
?
propionaldehyde + NAD+ + H2O
propanoate + NADH + H+
-
107% activity compared to 2-hydroxymuconate-6-semialdehyde
-
-
?
additional information
?
-
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
Q1XGK8
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
Q1XGK8
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
-
100% activity
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
-
the activity of crude enzyme for 2-hydroxymuconate-6-semialdehyde in the presence of NAD+ is about 11 times higher than that achieved with NADP+
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
-
the activity of crude enzyme for 2-hydroxymuconate-6-semialdehyde in the presence of NAD+ is about 11 times higher than that achieved with NADP+
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
-
-
-
-
?
5-chloro-2-hydroxymuconic semialdehyde + NAD+ + H2O
5-chloro-2-hydroxymuconic acid + NADH + H+
-
-
-
-
?
5-chloro-2-hydroxymuconic semialdehyde + NAD+ + H2O
5-chloro-2-hydroxymuconic acid + NADH + H+
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
-
25% activity compared to 2-hydroxymuconate-6-semialdehyde
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
-
weak substrate
-
-
?
additional information
?
-
Q1XGK8
enzyme NahI is highly specific for its biological substrate, 2-hydroxymuconate semialdehyde, no activity with salicylaldehyde, another intermediate in the naphthalene-degradation pathway
-
-
-
additional information
?
-
Q1XGK8
enzyme NahI is highly specific for its biological substrate, 2-hydroxymuconate semialdehyde, no activity with salicylaldehyde, another intermediate in the naphthalene-degradation pathway
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
Q1XGK8
-
-
-
?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
Q1XGK8
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NAD+
-
highly specific for NAD+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Cu2+
-
complete inhibition at 1 mM
Hg2+
-
complete inhibition at 1 mM
iodoacetic acid
-
69% residual activity at 1 mM
N-ethylmaleimide
-
18% residual activity at 1 mM
p-chloromercuribenzoate
-
41% residual activity at 1 mM
Zn2+
-
26% residual activity at 1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0093
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate
-
in 100 mM Tris-HCl, pH 8.5, at 25°C
0.0013 - 0.017
2-hydroxymuconate-6-semialdehyde
1.3
3-Chlorobenzaldehyde
-
in 100 mM glycine-NaOH (pH 9.2), at 25°C
0.17
3-Fluorobenzaldehyde
-
in 100 mM glycine-NaOH (pH 9.2), at 25°C
0.94
3-Methoxybenzaldehyde
-
in 100 mM glycine-NaOH (pH 9.2), at 25°C
0.63
3-methylbenzaldehyde
-
in 100 mM glycine-NaOH (pH 9.2), at 25°C
0.81
3-Nitrobenzaldehyde
-
in 100 mM glycine-NaOH (pH 9.2), at 25°C
0.53
4-methylbenzaldehyde
-
in 100 mM glycine-NaOH (pH 9.2), at 25°C
0.46
benzaldehyde
-
in 100 mM glycine-NaOH (pH 9.2), at 25°C
0.33
NAD+
-
in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25°C
additional information
additional information
Q1XGK8
Michaelis-Menten kinetics
-
0.0013
2-hydroxymuconate-6-semialdehyde
Q1XGK8
pH 8.5, 25°C, recombinant enzyme
0.017
2-hydroxymuconate-6-semialdehyde
-
in 100 mM Tris-HCl, pH 8.5, at 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.9
2-hydroxymuconate-6-semialdehyde
Q1XGK8
pH 8.5, 25°C, recombinant enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2100
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate
-
apparent value, in 100 mM Tris-HCl, pH 8.5, at 25°C
1600
2-hydroxymuconate-6-semialdehyde
-
apparent value, in 100 mM Tris-HCl, pH 8.5, at 25°C
22
3-Chlorobenzaldehyde
-
apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C
330
3-Fluorobenzaldehyde
-
apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C
8
3-Methoxybenzaldehyde
-
apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C
36
3-methylbenzaldehyde
-
apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C
20
3-Nitrobenzaldehyde
-
apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C
10
4-methylbenzaldehyde
-
apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C
32
benzaldehyde
-
apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C
56
NAD+
-
apparent value, in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
enzyme from cell extract shows a specific activity of 0.0041 units/mg in 100 mM sodium phosphate, pH 7.5, at 24°C. After 17fold purification, the enzyme shows a specific activity of 0.071 units/mg in 100 mM sodium phosphate, pH 7.5, at 24°C, in 100 mM sodium phosphate, pH 7.5, at 24°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8.3
-
pH optimum for the oxidation of 2-hydroxymuconic semialdehyde
9.6
-
pH optimum for the oxidation of benzaldehyde
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
Q1XGK8
UniProt
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
Q1XGK8
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
-
brenda
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
evolution
Q1XGK8
the naphthalene catabolic genes (nah) of NAH7 are organized into two operons. The nah operon (nahAaAbAcAdBFCED) encodes the upper pathway enzymes involved in the conversion of naphthalene to salicylate. On the other hand, the sal operon (nahGTHINLOMKJ) codes for the lower pathway enzymes involved in the conversion of salicylate to pyruvate and acetaldehyde. The lower pathway starts with the oxidation of salicylate to catechol by salicylate hydroxylase (NahG), which is extradiol-cleaved by catechol-2,3-dioxygenase (NahH) and further transformed to pyruvate and acetyl-CoA by the remaining meta-cleavage pathway gene products, NahI, NahJ, NahK, NahN, NahL, NahM, and NahO. Enzyme NahI is further classified into the ALDH8 family together with different 2-hydroxymuconate semialdehyde dehydrogenases
evolution
-
the naphthalene catabolic genes (nah) of NAH7 are organized into two operons. The nah operon (nahAaAbAcAdBFCED) encodes the upper pathway enzymes involved in the conversion of naphthalene to salicylate. On the other hand, the sal operon (nahGTHINLOMKJ) codes for the lower pathway enzymes involved in the conversion of salicylate to pyruvate and acetaldehyde. The lower pathway starts with the oxidation of salicylate to catechol by salicylate hydroxylase (NahG), which is extradiol-cleaved by catechol-2,3-dioxygenase (NahH) and further transformed to pyruvate and acetyl-CoA by the remaining meta-cleavage pathway gene products, NahI, NahJ, NahK, NahN, NahL, NahM, and NahO. Enzyme NahI is further classified into the ALDH8 family together with different 2-hydroxymuconate semialdehyde dehydrogenases
-
physiological function
Q1XGK8
the enzyme is involved in the degradation of intermediate 2-hydroxymuconate 6-semialdehyde in the naphthalene-degradation pathway
physiological function
-
the enzyme is involved in the degradation of intermediate 2-hydroxymuconate 6-semialdehyde in the naphthalene-degradation pathway
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
53188
-
x * 53188, calculated from amino acid sequence
58000
-
x * 58000, SDS-PAGE
184000
Q1XGK8
recombinant enzyme, gel filtration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 53188, calculated from amino acid sequence; x * 58000, SDS-PAGE
?
-
x * 53188, calculated from amino acid sequence; x * 58000, SDS-PAGE
-
tetramer
Q1XGK8
x * 51712, recombinant detagged enzyme, mass spectrometry, x * 55000, recombinant N-terminally His-tagged enzyme, SDS-PAGE
tetramer
-
x * 51712, recombinant detagged enzyme, mass spectrometry, x * 55000, recombinant N-terminally His-tagged enzyme, SDS-PAGE
-
additional information
Q1XGK8
enzyme protein structure analysis by circular dichroism spectroscopy, and three-dimensional structure model, overview
additional information
-
enzyme protein structure analysis by circular dichroism spectroscopy, and three-dimensional structure model, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
enzyme structure analysis by dynamic light scattering, small-angle X-ray scattering experiments and circular dichroism spectroscopy
Q1XGK8
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ammonium sulfate fractionation, TSKgel DEAE-5PW column chromatography, Affigel-Blue affinity chromatography, and TSKgel Phenyl-5PW column chromatography
-
recombinant soluble N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag cleavage by TEV protease, and another nickel affinity chromatography step, followed by gel filtration and ultrafiltration
Q1XGK8
streptomycin sulfate precipitation, ammonium sulfate precipitation, DE52 cellulose column chromatography, DEAE-cellulofine column chromatography, and DEAE-Toyopearl column chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) cells
-
gene nahI, the naphthalene catabolic genes (nah) of NAH7 are organized into two operons on a 83 kilobase plasmid, recombinant expression of soluble N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Q1XGK8
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Inoue, J.; Shaw, J.P.; Rekik, M.; Harayama, S.
Overlapping substrate specificities of benzaldehyde dehydrogenase (the xylC gene product) and 2-hydroxymuconic semialdehyde dehydrogenase (the xylG gene product) encoded by TOL plasmid pWW0 of Pseudomonas putida
J. Bacteriol.
177
1196-1201
1995
Pseudomonas putida, Pseudomonas putida KT 2240
brenda
Orii, C.; Takenaka, S.; Murakami, S.; Aoki, K.
Metabolism of 4-amino-3-hydroxybenzoic acid by Bordetella sp. strain 10d: a different modified meta-cleavage pathway for 2-aminophenols
Biosci. Biotechnol. Biochem.
70
2653-2661
2006
Bordetella sp. 10d, Bordetella sp.
brenda
Kasai, D.; Fujinami, T.; Abe, T.; Mase, K.; Katayama, Y.; Fukuda, M.; Masai, E.
Uncovering the protocatechuate 2,3-cleavage pathway genes
J. Bacteriol.
191
6758-6768
2009
Paenibacillus sp., Paenibacillus sp. JJ-1b
brenda
Park, S.; Lee, D.; Kim, Y.; Lee, K.; Kim, C.
Cloning and nucleotide sequence analysis of xylG gene encoding 5C-2HMS dehydrogenase from Pseudomonas sp. S-47
Korean J. Appl. Microbiol. Bioeng.
30
8-14
2002
Pseudomonas sp., Pseudomonas sp. S-47
-
brenda
de Araujo, S.; Neves, C.M.L.; Guimaraes, S.L.; Whitman, C.P.; Johnson Jr., W.H.; Aparicio, R.; Nagem, R.A.P.
Structural and kinetic characterization of recombinant 2-hydroxymuconate semialdehyde dehydrogenase from Pseudomonas putida G7
Arch. Biochem. Biophys.
579
8-17
2015
Pseudomonas putida (Q1XGK8), Pseudomonas putida G7 (Q1XGK8)
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
1.2.1.85 2-hydroxymuconate-6-semialdehyde dehydrogenase
more
top
Information
