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EC Tree
IUBMB Comments This substrate for this enzyme is formed by meta ring cleavage of catechol (EC 1.13.11.2, catechol 2,3-dioxygenase), and is an intermediate in the bacterial degradation of several aromatic compounds. Has lower activity with benzaldehyde . Activity with NAD+ is more than 10-fold higher than with NADP+ . cf. EC 1.2.1.32, aminomuconate-semialdehyde dehydrogenase.
The enzyme appears in viruses and cellular organisms
Synonyms
2-hydroxymuconate semialdehyde dehydrogenase, 2-hydroxymuconic semialdehyde dehydrogenase, 5C-2HMS dehydrogenase, HMS dehydrogenase, NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase,
NahI ,
praB ,
XylG ,
more
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2-hydroxymuconate semialdehyde dehydrogenase
2-hydroxymuconic semialdehyde dehydrogenase
NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase
2-hydroxymuconate semialdehyde dehydrogenase
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2-hydroxymuconate semialdehyde dehydrogenase
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2-hydroxymuconic semialdehyde dehydrogenase
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2-hydroxymuconic semialdehyde dehydrogenase
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5C-2HMS dehydrogenase
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5C-2HMS dehydrogenase
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HMS dehydrogenase
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NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase
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NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase
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NahI
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praB
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XylG
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2-hydroxymuconate-6-semialdehyde + NAD+ + H2O = (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
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2-hydroxymuconate-6-semialdehyde:NAD+ oxidoreductase
This substrate for this enzyme is formed by meta ring cleavage of catechol (EC 1.13.11.2, catechol 2,3-dioxygenase), and is an intermediate in the bacterial degradation of several aromatic compounds. Has lower activity with benzaldehyde [1]. Activity with NAD+ is more than 10-fold higher than with NADP+ [3]. cf. EC 1.2.1.32, aminomuconate-semialdehyde dehydrogenase.
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2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O
(2E,4Z)-2-hydroxy-5-methylhexa-2,4-diendioate + NADH + H+
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?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
3-chlorobenzaldehyde + NAD+ + H2O
3-chlorobenzoate + NADH + H+
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?
3-fluorobenzaldehyde + NAD+ + H2O
3-fluorobenzoate + NADH + H+
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?
3-methoxybenzaldehyde + NAD+ + H2O
3-methoxybenzoate + NADH + H+
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?
3-methylbenzaldehyde + NAD+ + H2O
3-methylbenzoate + NADH + H+
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?
3-nitrobenzaldehyde + NAD+ + H2O
3-nitrobenzoate + NADH + H+
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?
4-methylbenzaldehyde + NAD+ + H2O
4-methylbenzoate + NADH + H+
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?
5-chloro-2-hydroxymuconic semialdehyde + NAD+ + H2O
5-chloro-2-hydroxymuconic acid + NADH + H+
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
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113% activity compared to 2-hydroxymuconate-6-semialdehyde
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?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
butyraldehyde + NAD+ + H2O
butanoate + NADH + H+
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138% activity compared to 2-hydroxymuconate-6-semialdehyde
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formaldehyde + NAD+ + H2O
formate + NADH + H+
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16.5% activity compared to 2-hydroxymuconate-6-semialdehyde
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?
propionaldehyde + NAD+ + H2O
propanoate + NADH + H+
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107% activity compared to 2-hydroxymuconate-6-semialdehyde
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?
additional information
?
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2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
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?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
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?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
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100% activity
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2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
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the activity of crude enzyme for 2-hydroxymuconate-6-semialdehyde in the presence of NAD+ is about 11 times higher than that achieved with NADP+
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2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
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the activity of crude enzyme for 2-hydroxymuconate-6-semialdehyde in the presence of NAD+ is about 11 times higher than that achieved with NADP+
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2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
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5-chloro-2-hydroxymuconic semialdehyde + NAD+ + H2O
5-chloro-2-hydroxymuconic acid + NADH + H+
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5-chloro-2-hydroxymuconic semialdehyde + NAD+ + H2O
5-chloro-2-hydroxymuconic acid + NADH + H+
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benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
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25% activity compared to 2-hydroxymuconate-6-semialdehyde
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benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
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weak substrate
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additional information
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enzyme NahI is highly specific for its biological substrate, 2-hydroxymuconate semialdehyde, no activity with salicylaldehyde, another intermediate in the naphthalene-degradation pathway
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additional information
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enzyme NahI is highly specific for its biological substrate, 2-hydroxymuconate semialdehyde, no activity with salicylaldehyde, another intermediate in the naphthalene-degradation pathway
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2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
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2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+
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?
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NAD+
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NAD+
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highly specific for NAD+
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Cu2+
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complete inhibition at 1 mM
Hg2+
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complete inhibition at 1 mM
iodoacetic acid
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69% residual activity at 1 mM
N-ethylmaleimide
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18% residual activity at 1 mM
p-chloromercuribenzoate
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41% residual activity at 1 mM
Zn2+
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26% residual activity at 1 mM
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0.0093
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate
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in 100 mM Tris-HCl, pH 8.5, at 25ưC
0.0013 - 0.017
2-hydroxymuconate-6-semialdehyde
1.3
3-Chlorobenzaldehyde
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in 100 mM glycine-NaOH (pH 9.2), at 25ưC
0.17
3-Fluorobenzaldehyde
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in 100 mM glycine-NaOH (pH 9.2), at 25ưC
0.94
3-Methoxybenzaldehyde
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in 100 mM glycine-NaOH (pH 9.2), at 25ưC
0.63
3-methylbenzaldehyde
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in 100 mM glycine-NaOH (pH 9.2), at 25ưC
0.81
3-Nitrobenzaldehyde
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in 100 mM glycine-NaOH (pH 9.2), at 25ưC
0.53
4-methylbenzaldehyde
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in 100 mM glycine-NaOH (pH 9.2), at 25ưC
0.46
benzaldehyde
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in 100 mM glycine-NaOH (pH 9.2), at 25ưC
0.33
NAD+
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in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25ưC
additional information
additional information
Michaelis-Menten kinetics
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0.0013
2-hydroxymuconate-6-semialdehyde
pH 8.5, 25ưC, recombinant enzyme
0.017
2-hydroxymuconate-6-semialdehyde
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in 100 mM Tris-HCl, pH 8.5, at 25ưC
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0.9
2-hydroxymuconate-6-semialdehyde
pH 8.5, 25ưC, recombinant enzyme
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2100
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate
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apparent value, in 100 mM Tris-HCl, pH 8.5, at 25ưC
1600
2-hydroxymuconate-6-semialdehyde
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apparent value, in 100 mM Tris-HCl, pH 8.5, at 25ưC
22
3-Chlorobenzaldehyde
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apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25ưC
330
3-Fluorobenzaldehyde
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apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25ưC
8
3-Methoxybenzaldehyde
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apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25ưC
36
3-methylbenzaldehyde
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apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25ưC
20
3-Nitrobenzaldehyde
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apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25ưC
10
4-methylbenzaldehyde
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apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25ưC
32
benzaldehyde
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apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25ưC
56
NAD+
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apparent value, in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25ưC
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additional information
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enzyme from cell extract shows a specific activity of 0.0041 units/mg in 100 mM sodium phosphate, pH 7.5, at 24ưC. After 17fold purification, the enzyme shows a specific activity of 0.071 units/mg in 100 mM sodium phosphate, pH 7.5, at 24ưC, in 100 mM sodium phosphate, pH 7.5, at 24ưC
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8.3
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pH optimum for the oxidation of 2-hydroxymuconic semialdehyde
9.6
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pH optimum for the oxidation of benzaldehyde
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UniProt
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UniProt
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evolution
the naphthalene catabolic genes (nah) of NAH7 are organized into two operons. The nah operon (nahAaAbAcAdBFCED) encodes the upper pathway enzymes involved in the conversion of naphthalene to salicylate. On the other hand, the sal operon (nahGTHINLOMKJ) codes for the lower pathway enzymes involved in the conversion of salicylate to pyruvate and acetaldehyde. The lower pathway starts with the oxidation of salicylate to catechol by salicylate hydroxylase (NahG), which is extradiol-cleaved by catechol-2,3-dioxygenase (NahH) and further transformed to pyruvate and acetyl-CoA by the remaining meta-cleavage pathway gene products, NahI, NahJ, NahK, NahN, NahL, NahM, and NahO. Enzyme NahI is further classified into the ALDH8 family together with different 2-hydroxymuconate semialdehyde dehydrogenases
evolution
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the naphthalene catabolic genes (nah) of NAH7 are organized into two operons. The nah operon (nahAaAbAcAdBFCED) encodes the upper pathway enzymes involved in the conversion of naphthalene to salicylate. On the other hand, the sal operon (nahGTHINLOMKJ) codes for the lower pathway enzymes involved in the conversion of salicylate to pyruvate and acetaldehyde. The lower pathway starts with the oxidation of salicylate to catechol by salicylate hydroxylase (NahG), which is extradiol-cleaved by catechol-2,3-dioxygenase (NahH) and further transformed to pyruvate and acetyl-CoA by the remaining meta-cleavage pathway gene products, NahI, NahJ, NahK, NahN, NahL, NahM, and NahO. Enzyme NahI is further classified into the ALDH8 family together with different 2-hydroxymuconate semialdehyde dehydrogenases
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physiological function
the enzyme is involved in the degradation of intermediate 2-hydroxymuconate 6-semialdehyde in the naphthalene-degradation pathway
physiological function
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the enzyme is involved in the degradation of intermediate 2-hydroxymuconate 6-semialdehyde in the naphthalene-degradation pathway
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184000
recombinant enzyme, gel filtration
53188
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x * 53188, calculated from amino acid sequence
58000
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x * 58000, SDS-PAGE
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?
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x * 58000, SDS-PAGE
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x * 53188, calculated from amino acid sequence
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x * 58000, SDS-PAGE
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?
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x * 53188, calculated from amino acid sequence
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tetramer
x * 51712, recombinant detagged enzyme, mass spectrometry, x * 55000, recombinant N-terminally His-tagged enzyme, SDS-PAGE
tetramer
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x * 51712, recombinant detagged enzyme, mass spectrometry, x * 55000, recombinant N-terminally His-tagged enzyme, SDS-PAGE
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additional information
enzyme protein structure analysis by circular dichroism spectroscopy, and three-dimensional structure model, overview
additional information
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enzyme protein structure analysis by circular dichroism spectroscopy, and three-dimensional structure model, overview
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enzyme structure analysis by dynamic light scattering, small-angle X-ray scattering experiments and circular dichroism spectroscopy
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ammonium sulfate fractionation, TSKgel DEAE-5PW column chromatography, Affigel-Blue affinity chromatography, and TSKgel Phenyl-5PW column chromatography
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recombinant soluble N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag cleavage by TEV protease, and another nickel affinity chromatography step, followed by gel filtration and ultrafiltration
streptomycin sulfate precipitation, ammonium sulfate precipitation, DE52 cellulose column chromatography, DEAE-cellulofine column chromatography, and DEAE-Toyopearl column chromatography
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expressed in Escherichia coli BL21(DE3) cells
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gene nahI, the naphthalene catabolic genes (nah) of NAH7 are organized into two operons on a 83 kilobase plasmid, recombinant expression of soluble N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
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Inoue, J.; Shaw, J.P.; Rekik, M.; Harayama, S.
Overlapping substrate specificities of benzaldehyde dehydrogenase (the xylC gene product) and 2-hydroxymuconic semialdehyde dehydrogenase (the xylG gene product) encoded by TOL plasmid pWW0 of Pseudomonas putida
J. Bacteriol.
177
1196-1201
1995
Pseudomonas putida, Pseudomonas putida KT 2240
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Orii, C.; Takenaka, S.; Murakami, S.; Aoki, K.
Metabolism of 4-amino-3-hydroxybenzoic acid by Bordetella sp. strain 10d: a different modified meta-cleavage pathway for 2-aminophenols
Biosci. Biotechnol. Biochem.
70
2653-2661
2006
Bordetella sp., Bordetella sp. 10d
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Kasai, D.; Fujinami, T.; Abe, T.; Mase, K.; Katayama, Y.; Fukuda, M.; Masai, E.
Uncovering the protocatechuate 2,3-cleavage pathway genes
J. Bacteriol.
191
6758-6768
2009
Paenibacillus sp., Paenibacillus sp. JJ-1b
brenda
Park, S.; Lee, D.; Kim, Y.; Lee, K.; Kim, C.
Cloning and nucleotide sequence analysis of xylG gene encoding 5C-2HMS dehydrogenase from Pseudomonas sp. S-47
Korean J. Appl. Microbiol. Bioeng.
30
8-14
2002
Pseudomonas sp., Pseudomonas sp. S-47
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de Araujo, S.; Neves, C.M.L.; Guimaraes, S.L.; Whitman, C.P.; Johnson Jr., W.H.; Aparicio, R.; Nagem, R.A.P.
Structural and kinetic characterization of recombinant 2-hydroxymuconate semialdehyde dehydrogenase from Pseudomonas putida G7
Arch. Biochem. Biophys.
579
8-17
2015
Pseudomonas putida (Q1XGK8), Pseudomonas putida G7 (Q1XGK8)
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