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The enzyme appears in viruses and cellular organisms
Synonyms dehydrogenase, malonate semialdehyde, IolA, KES23460 , NAD-dependent malonate-semialdehyde dehydrogenase, more
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dehydrogenase, malonate semialdehyde
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NAD-dependent malonate-semialdehyde dehydrogenase
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3-oxopropanoate + NAD(P)+ + H2O = malonate + NAD(P)H + H+
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3-oxopropanoate:NAD(P)+ oxidoreductase
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3-oxopropanoate + NAD+ + H2O
malonate + NADH
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Substrates: - Products: -
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malonic semialdehyde + NAD+
acetyl-CoA + NADH + CO2
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Substrates: - Products: -
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NAD(P)+ + 3-oxopropanoate + H2O
malonate + NAD(P)H
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Substrates: - Products: -
ir
additional information
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Substrates: no activity with formaldehyde, acetaldehyde, propionaldehyde, isovaleraldehyde, benzaldehyde, anisaldehyde, glyceraldehyde, glyceraldehyde-3-phosphate, glyoxylate, succinic semialdehyde Products: -
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3-oxopropanoate + NAD+ + H2O
malonate + NADH
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Substrates: - Products: -
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NADP+
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less than 10% activity compared with NAD+
NAD+
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Ca2+
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increases activity 42% at 1 mM
Mg2+
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increases activity 64% at 1 mM
Mn2+
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increases activity 27% at 1 mM
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glyoxylate
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competitive inhibitor
malonate
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competitive inhibitor
p-chloromercuribenzoate
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succinate semialdehyde
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non-competitive inhibitor
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0.043
malonate semialdehyde
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0.56
succinate semialdehyde
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8.7
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malonate semialdehyde + NAD+
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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physiological function
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the enzyme is involved in the beta-alanine catabolism to produce acetyl-CoA
physiological function
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the enzyme is involved in the beta-alanine catabolism to produce acetyl-CoA
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hexamer
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purified recombinant enzyme, method screening, crystallization of 20 mg/ml protein with crystalliztaion solution consisting of 1.38 M ammonium sulfate, 3.35% 2,2,2-trifluoroethanol, 2% benzamidine, the resulting crystals are used for microseding with 5 mg/ml protein, 1 mM acetyl-CoA, and a solution containing 23.7% w/v PEG 3350, 0.208 M trisodium citrate, 0.1 M Bis-Tris propane, pH 7.55, at 8°C, method optimization, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement using structure with PDB ID 4zz7 as the search model
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additional information
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disruption of iolA gene provides a myo-inositol-negative strain
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with NAD+ and bovine serum albumin at pH 8.7
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1 mM mercaptoethanol is essential for stability
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unstable in dilute solution
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-20°C, purified enzyme, 1 month
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0°C, bovine serum albumin, NAD+, 1 week
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recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration to over 95% purity
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gene KES23460, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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mutated iolA gene cloned into pRV300 giving pRViolASpeI. Expressed in Lactobacillus casei
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additional information
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iolA is indispensable for myo-inositol fermentation
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Nakamura, K.; Bernheim, F.
Studies on malonic semialdehyde dehydrogenase from Pseudomonas aeruginosa
Biochim. Biophys. Acta
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147-152
1961
Pseudomonas aeruginosa
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Yebra, M.J.; Zuniga, M.; Beaufils, S.; Perez-Martinez, G.; Deutscher, J.; Monedero, V.
Identification of a gene cluster enabling Lactobacillus casei BL23 to utilize myo-inositol
Appl. Environ. Microbiol.
73
3850-3858
2007
Lacticaseibacillus casei
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Wilding, M.; Scott, C.; Peat, T.S.; Newman, J.
X-ray crystal structure of a malonate-semialdehyde dehydrogenase from Pseudomonas sp. strain AAC
Acta Crystallogr. Sect. F
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24-28
2017
Pseudomonas sp., Pseudomonas sp. AAC
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