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Information on EC 1.2.1.103 - [amino group carrier protein]-6-phospho-L-2-aminoadipate reductase for references in articles please use BRENDA:EC1.2.1.103
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EC Tree
IUBMB Comments The enzyme participates in an L-lysine biosynthesis in certain species of archaea and bacteria.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
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an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine
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+
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lysY
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an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + phosphate + NADP+ = an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + NADPH + H+
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[amino group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine:NADP+ 5-oxidoreductase (phosphorylating)
The enzyme participates in an L-lysine biosynthesis in certain species of archaea and bacteria.
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an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + NADPH + H+
an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + phosphate + NADP+
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-
-
r
[amino group carrier protein LysW]-gamma-alpha-aminoadipate + NADPH + H+
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde + phosphate + NADP+
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-
r
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde + phosphate + NADP+
[amino group carrier protein LysW]-gamma-alpha-aminoadipate phosphate + NADPH + H+
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r
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an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + NADPH + H+
an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + phosphate + NADP+
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-
-
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r
[amino group carrier protein LysW]-gamma-alpha-aminoadipate + NADPH + H+
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde + phosphate + NADP+
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-
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r
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde + phosphate + NADP+
[amino group carrier protein LysW]-gamma-alpha-aminoadipate phosphate + NADPH + H+
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r
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ATP
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20 mM used in assay conditions
pyridoxal 5'-phosphate
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5 mM used in assay conditions
NADP+
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-
NADPH
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-
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Co2+
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0.01 mM used in assay conditions
Mg2+
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0.01 mM used in assay conditions
Zn2+
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0.01 mM used in assay conditions
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0.014
NADP+
wild type enzyme, at pH 9.5 and 60°C
12
phosphate
wild type enzyme, at pH 9.5 and 60°C
0.0078 - 0.69
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
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0.0078
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme H209A, at pH 7.5 and 60°C
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0.0098
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
wild type enzyme, at pH 9.5 and 60°C
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0.011
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme H209A, at pH 9.5 and 60°C
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0.011
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
wild type enzyme, at pH 7.5 and 60°C
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0.037
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme R102A, at pH 9.5 and 60°C
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0.17
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme R258A, at pH 9.5 and 60°C
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0.39
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme R195A, at pH 9.5 and 60°C
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0.69
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme K271A, at pH 9.5 and 60°C
-
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1.2
NADP+
wild type enzyme, at pH 9.5 and 60°C
1.1
phosphate
wild type enzyme, at pH 9.5 and 60°C
0.0063 - 0.96
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
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0.0063
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme R102A, at pH 9.5 and 60°C
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0.0082
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme H209A, at pH 7.5 and 60°C
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0.011
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
wild type enzyme, at pH 7.5 and 60°C
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0.19
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme R195A, at pH 9.5 and 60°C
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0.21
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme K271A, at pH 9.5 and 60°C
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0.5
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme H209A, at pH 9.5 and 60°C
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0.66
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme R258A, at pH 9.5 and 60°C
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0.96
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
wild type enzyme, at pH 9.5 and 60°C
-
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81
NADP+
wild type enzyme, at pH 9.5 and 60°C
0.0089
phosphate
wild type enzyme, at pH 9.5 and 60°C
0.17 - 98
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
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0.17
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme R102A, at pH 9.5 and 60°C
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0.31
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme K271A, at pH 9.5 and 60°C
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0.49
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme R195A, at pH 9.5 and 60°C
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1.1
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme H209A, at pH 7.5 and 60°C
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4
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme R258A, at pH 9.5 and 60°C
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15
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
wild type enzyme, at pH 7.5 and 60°C
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47
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
mutant enzyme H209A, at pH 9.5 and 60°C
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98
[amino group carrier protein LysW]-gamma-aminoadipic semialdehyde
wild type enzyme, at pH 9.5 and 60°C
-
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brenda
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UniProt
brenda
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?
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x * about 40000, SDS-PAGE
homotetramer
4 * 38000, SDS-PAGE
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in complex with amino group carrier protein LysW-gamma-alpha-aminoadipate plus NADP+ and amino group carrier protein LysW-gamma-aminoadipic semialdehyde plus NADP+, hanging drop vapor diffusion method, using 20% (w/v) PEG 2000 monomethyl ester and 200 mM calcium acetate
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H209A
the mutant exhibits similar activity to that of the wildtype enzyme
K271A
the mutation results in a 4.6fold decrease in kcat and a 70fold increase in Km value compared to the wild type enzyme
R195A
the mutation results in a 5fold decrease in kcat and a 40fold increase in Km value compared to the wild type enzyme
R258A
the mutation results in a slight decrease in kcat and a 17fold increase in Km value compared to the wild type enzyme
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Blue-Sepharose column chromatography and Superdex S200 gel filtration
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expressed in Escherichia coli
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expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
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Horie, A.; Tomita, T.; Saiki, A.; Kono, H.; Taka, H.; Mineki, R.; Fujimura, T.; Nishiyama, C.; Kuzuyama, T.; Nishiyama, M.
Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus
Nat. Chem. Biol.
5
673-679
2009
Thermus thermophilus
brenda
Shimizu, T.; Tomita, T.; Kuzuyama, T.; Nishiyama, M.
Crystal structure of the LysY.LysW complex from Thermus thermophilus
J. Biol. Chem.
291
9948-9959
2016
Thermus thermophilus (O50146)
brenda
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Enzyme Nomenclature
1.2.1.103 [amino group carrier protein]-6-phospho-L-2-aminoadipate reductase
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