Information on EC 1.18.6.1 - nitrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

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EC NUMBER
COMMENTARY hide
1.18.6.1
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RECOMMENDED NAME
GeneOntology No.
nitrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
nitrogen fixation I (ferredoxin)
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nitrate assimilation
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Chloroalkane and chloroalkene degradation
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Nitrogen metabolism
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
reduced ferredoxin:dinitrogen oxidoreductase (ATP-hydrolysing)
Requires Mg2+. It is composed of two proteins that can be separated but are both required for nitrogenase activity. Dinitrogen reductase is a [4Fe-4S] protein, which, with two molecules of ATP and ferredoxin, generates an electron. The electron is transferred to the other protein, dinitrogenase (molybdoferredoxin). Dinitrogenase is a molybdenum-iron protein that reduces dinitrogen in three succesive two-electron reductions from nitrogen to diimine to hydrazine to two molecules of ammonia. The molybdenum may be replaced by vanadium or iron. The reduction is initiated by formation of hydrogen in stoichiometric amounts [2]. Acetylene is reduced to ethylene (but only very slowly to ethane), azide to nitrogen and ammonia, and cyanide to methane and ammonia. In the absence of a suitable substrate, hydrogen is slowly formed. Ferredoxin may be replaced by flavodoxin [see EC 1.19.6.1 nitrogenase (flavodoxin)].
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CAS REGISTRY NUMBER
COMMENTARY hide
9013-04-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
enzyme activity increases with increasing concentration of O2 in the root zone. Photosynthetic rate, plant dry mass, leaf N content, and nodule fresh mass are maximal in plants maintained with 15-25% O2 in the root zone
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
contains Mo- and V-nitrogenases
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Chromatium sp.
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Manually annotated by BRENDA team
a marine unicellular diazotrophic cyanobacterium
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Manually annotated by BRENDA team
a marine unicellular diazotrophic cyanobacterium
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Manually annotated by BRENDA team
Cyanobacterium sp.
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Manually annotated by BRENDA team
low activity
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
C-M 74
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Manually annotated by BRENDA team
C-M 74
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Gloeocapsa sp.
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Manually annotated by BRENDA team
a terrestrial unicellular diazotrophic cyanobacterium
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Manually annotated by BRENDA team
in endosymbiosis with sugarcane, Saccharum officinarum
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Manually annotated by BRENDA team
inoculated with symbiont Mesorhizobium loti strain New Zealand Palmerston 2235
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
NifH2 is one of two homologues of NifH in Methanocaldococcus jannaschii
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ORS571
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Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
physiological function
additional information
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(VO4)3- + ?
(VO2)+ + ?
show the reaction diagram
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reduction of vanadium(V) by reduced Fe-protein of enzyme to vanadium(IV), which then probably binds to the nucleotide binding site in place of the Mg2+ which is normally present. The oxidized Fe-protein is unable to reduce vanadate
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?
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
show the reaction diagram
3,3-difluorocyclopropene + dithionite
propene + 2-fluoropropene + ?
show the reaction diagram
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
show the reaction diagram
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
show the reaction diagram
acetylene + ?
ethylene + ?
show the reaction diagram
C2H2 + ?
?
show the reaction diagram
C2H2 + ?
H2 + ?
show the reaction diagram
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?
CN- + europium(II) diethylenetriaminepentaacetate + H+
?
show the reaction diagram
CO + dithionite + H+
CH4 + C2H6 + C2H4 + C3H6 + C3H8 + ?
show the reaction diagram
CO + europium(II) diethylenetriaminepentaacetate + H+
?
show the reaction diagram
CS2 + ?
H2S + ?
show the reaction diagram
slow turnover
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?
cyanamide + ?
?
show the reaction diagram
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?
cyanide + ?
?
show the reaction diagram
cyclopropene + ?
?
show the reaction diagram
diazene + ?
?
show the reaction diagram
dithionite + H+ + acetylene + ATP
?
show the reaction diagram
dithionite + H+ + N2 + ATP
?
show the reaction diagram
ethylene + ?
?
show the reaction diagram
hydrazine + ?
?
show the reaction diagram
hydrazine + reduced ferredoxin
2 NH3 + oxidized ferredoxin
show the reaction diagram
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?
hydroxylamine + reduced ferredoxin
NH3 + H2O + oxidized ferredoxin
show the reaction diagram
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?
methyl isocyanide + ?
?
show the reaction diagram
methyldiazene + ?
?
show the reaction diagram
N2 + 4 reduced ferredoxin
hydrazine + 4 oxidized ferredoxin
show the reaction diagram
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?
N2 + 8 e- + 16 ATP + 8 H+
2 NH3 + H2 + 16 ADP + 16 phosphate
show the reaction diagram
N2 + 8 e- + 8 H+ + 16 ATP
2 NH3 + H2 + 16 ADP + 16 phosphate
show the reaction diagram
N2H4 + ?
?
show the reaction diagram
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?
N2O + ?
?
show the reaction diagram
N3- + ?
?
show the reaction diagram
nitrite + 4 reduced ferredoxin + 5 H+
hydroxylamine + H2O + 4 oxidized ferredoxin
show the reaction diagram
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?
nitrite + 7 H+ + 12 ATP
NH3 + 2 H2O + 12 ADP + 12 phosphate
show the reaction diagram
overall reaction
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?
nitrite + H+ + ATP + reduced ferredoxin
NH3 + 2 H2O + 12 ADP + 12 phosphate + oxidized ferredoxin
show the reaction diagram
overall reaction
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?
propargyl alcohol + ?
?
show the reaction diagram
reduced ferredoxin + H+ + ATP
oxidized ferredoxin + H2 + ADP + phosphate
show the reaction diagram
reduced ferredoxin + H+ + CH3NC + ATP
oxidized ferredoxin + CH4 + C2H4 + C3H6 + C3H8 + CH3NH2 + ADP + phosphate
show the reaction diagram
reduced ferredoxin + H+ + CN- + ATP
oxidized ferredoxin + CH4 + NH3 + ADP + phosphate
show the reaction diagram
reduced ferredoxin + H+ + N2 + ATP
oxidized ferredoxin + H2 + NH3 + ADP + phosphate
show the reaction diagram
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?
reduced ferredoxin + H+ + N2 + ATP + H2O
oxidized ferredoxin + H2 + NH3 + ADP + phosphate
show the reaction diagram
reduced ferredoxin + H+ + N2O + ATP
oxidized ferredoxin + H2O + N2 + ADP + phosphate
show the reaction diagram
reduced ferredoxin + H+ + N3- + ATP
oxidized ferredoxin + NH3 + N2 + ADP + phosphate
show the reaction diagram
reduced ferredoxin + H+ + SCN- + ATP
oxidized ferredoxin + H2S + HCN + ADP + phosphate
show the reaction diagram
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?
Ti3+ + H+ + N2 + ATP
?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
show the reaction diagram
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
show the reaction diagram
hydrazine + reduced ferredoxin
2 NH3 + oxidized ferredoxin
show the reaction diagram
P00459
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?
hydroxylamine + reduced ferredoxin
NH3 + H2O + oxidized ferredoxin
show the reaction diagram
P00459
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?
N2 + 4 reduced ferredoxin
hydrazine + 4 oxidized ferredoxin
show the reaction diagram
P00459
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?
N2 + 8 e- + 16 ATP + 8 H+
2 NH3 + H2 + 16 ADP + 16 phosphate
show the reaction diagram
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the enzyme is responsible for biological nitrogen fixation, the conversion of atmospheric N2 to NH3
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?
N2 + 8 e- + 8 H+ + 16 ATP
2 NH3 + H2 + 16 ADP + 16 phosphate
show the reaction diagram
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ir
nitrite + 4 reduced ferredoxin + 5 H+
hydroxylamine + H2O + 4 oxidized ferredoxin
show the reaction diagram
P00459
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?
nitrite + H+ + ATP + reduced ferredoxin
NH3 + 2 H2O + 12 ADP + 12 phosphate + oxidized ferredoxin
show the reaction diagram
P00459
overall reaction
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?
reduced ferredoxin + H+ + N2 + ATP
oxidized ferredoxin + H2 + NH3 + ADP + phosphate
show the reaction diagram
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?
reduced ferredoxin + H+ + N2 + ATP + H2O
oxidized ferredoxin + H2 + NH3 + ADP + phosphate
show the reaction diagram
additional information
?
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