Information on EC 1.16.1.9 - ferric-chelate reductase (NADPH)

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Substrates: -
Products: -

?

2 Fe(II)-cytochrome c + NADP+ + H+

2 Fe(III)-cytochrome c + NADPH

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Substrates: -
Products: -

?

2 Fe(II)-siderophore + NADP+ + H+

2 Fe(III)-siderophore + NADPH

2 Fe(III)-(1,10-phenanthroline)3 + NADPH

2 Fe(II)-(1,10-phenanthroline)3 + NADP+ + H+

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Substrates: -
Products: -

?

2 Fe(III)-(catechol)2 + NADPH

2 Fe(II)-(catechol)2 + NADP+ + H+

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Substrates: -
Products: -

?

2 Fe(III)-(citrate)2 + NADPH

2 Fe(II)-(citrate)2 + NADP+ + H+

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Substrates: -
Products: -

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2 Fe(III)-(L-DOPA)2 + NADPH

2 Fe(II)-(L-DOPA)2 + NADP+ + H+

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Substrates: highest specific activity
Products: -

?

2 Fe(III)-citrate + NADPH

2 Fe(II)-citrate + NADP+ + H+

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Substrates: -
Products: -

?

2 Fe(III)-cyclam + NADPH

2 Fe(II)-cyclam + NADP+ + H+

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Substrates: lowest specific activity
Products: -

?

2 Fe(III)-dicitrate + NADPH

2 Fe(II)-dicitrate + NADP+ + H+

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766327

Substrates: -
Products: -

?

2 Fe(III)-diethylenetriaminepentaacetate + NADPH

2 Fe(II)-diethylenetriaminepentaacetate + NADP+ + H+

-

Substrates: -
Products: -

?

2 Fe(III)-EDTA + NADH

2 Fe(II)-EDTA + NAD+ + H+

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766327

Substrates: -
Products: ?

?

2 Fe(III)-EDTA + NADPH

2 Fe(II)-EDTA + NADP+ + H+

2 Fe(III)-nitrilotriacetic acid + NADPH

2 Fe(II)-nitrilotriacetic acid + NADP+ + H+

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Substrates: -
Products: -

?

2 Fe(III)-siderophore + NADPH

2 Fe(II)-siderophore + NADP+ + H+

2 Fe(III)-tris(2-pyridylmethyl)amine + NADPH

2 Fe(II)-tris(2-pyridylmethyl)amine + NADP+ + H+

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Substrates: -
Products: -

?

2 ferricyanide + NADPH

2 ferrocyanide + NADP+ + H+

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Substrates: -
Products: -

?

2 ferrocyanide + NADP+ + H+

2 ferricyanide + NADPH

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Substrates: -
Products: -

?

2,6-dichloroindophenol + NADPH + H+

?

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Substrates: best substrate in the presence and absence of FAD
Products: -

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an Fe(III)-siderophore + NADPH

Fe(II) + an apo-siderophore + NADP+ + H+

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Substrates: -
Products: -

?

cytochrome c + NADPH + H+

?

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Substrates: -
Products: -

?

Fe(II) + an apo-siderophore + NADP+ + H+

an Fe(III)-siderophore + NADPH

-

Substrates: -
Products: -

?

Fe(III)-(N-2,3-dihydroxybenzoyl-Gly-Thr)3 + NADPH + H+

Fe(II) + (2,3-dihydroxybenzoyl-Gly-Thr)3 + NADP+

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Substrates: -
Products: -

?

Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3 + NADPH + H+

Fe(II) + (2,3-dihydroxybenzoyl-L-serine)3 + NADP+

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Substrates: -
Products: -

?

Fe(III)-aerobactin + NADPH + H+

Fe(II) + aerobactin + NADP+

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Substrates: -
Products: -

?

Fe(III)-bacillibactin + NADPH + H+

Fe(II) + bacillibacitin + NADP+

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Substrates: -
Products: -

?

Fe(III)-citrate + NADPH + H+

?

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Substrates: -
Products: -

?

Fe(III)-deferoxamine + NADPH + H+

?

-

Substrates: -
Products: -

?

Fe(III)-dicitrate + NADPH + H+

Fe(II) + citrate + NADP+

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Substrates: -
Products: -

?

Fe(III)-diethylenetriamine-N,N,N',N'',N''-pentaacetate + NADPH + H+

Fe(II) + diethylenetriamine-N,N,N',N'',N''-pentaacetate + NADP+ + H+

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Substrates: -
Products: -

?

Fe(III)-EDTA + NADPH + H+

Fe(II) + EDTA + NADP+

Fe(III)-EDTA + NADPH + H+

Fe(II) + EDTA + NADP+ + H+

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Substrates: -
Products: -

?

Fe(III)-enterobactin + NADPH + H+

Fe(II) + enterobactin + NADP+

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Substrates: -
Products: -

?

Fe(III)-ferrichrome + NADPH + H+

?

-

Substrates: -
Products: -

?

Fe(III)-nitrilotriacetic acid + NADPH + H+

?

-

Substrates: -
Products: -

?

Fe(III)-o,o-EDDHA I + NADPH

Fe(II)-o,o-EDDHA I + NADP+ + H+

Cucumis sativus

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728418

Substrates: i.e. ethylenediaminebis(o-hydroxyphenyl)acetic acid
Products: -

?

Fe(III)-vibriobactin + NADPH + H+

Fe(II) + vibriobactin + NADP+

-

Substrates: -
Products: -

?

Fe2+ + NADPH

Fe3+ + NADP+ + H+

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766327

Substrates: -
Products: -

?

ferric ammonium citrate + NADPH + H+

?

-

Substrates: -
Products: -

?

ferric enterobactin + NADPH + H+

?

ferritin + NADPH + H+

?

-

Substrates: -
Products: -

?

tert-butyl hydroperoxide + NADPH + H+

?

-

Substrates: -
Products: -

?

transferrin + NADPH + H+

?

-

Substrates: -
Products: -

?

additional information

?

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6 entries

2 Fe(II)-siderophore + NADP+ + H+

2 Fe(III)-siderophore + NADPH

2 Fe(III)-siderophore + NADPH

2 Fe(II)-siderophore + NADP+ + H+

additional information

?

-

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Substrates: DNA degradation occurring in the presence of NADPH, Fe(III)-EDTA and hydrogen peroxide is potently enhanced by the purified enzyme, indicating that the enzyme may drive the Fenton reaction, reducing ferric iron to ferrous iron when it evokes the Fenton reaction
Products: -

?

FAD

7 entries

flavocytochrome b

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676551

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NADP+

Cucumis sativus

-

dependent on

728418

NADPH

10 entries

additional information

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the enzyme is flavin-independent

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Mg2+

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2 mM used in assay conditions

flavin

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the enzyme is able to reduce iron compounds in the absence of free flavin, but the ferric reduction by the enzyme is enhanced by the addition of free flavin n the presence of natural chelate iron compounds but also synthetic chelate iron compounds

0.0042

Fe(III)-(N-2,3-dihydroxybenzoyl-Gly-Thr)3

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wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C

0.0018 - 0.04

Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3

0.048

Fe(III)-aerobactin

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wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C

0.0276 - 0.16

Fe(III)-citrate

5 entries

0.0134 - 0.066

Fe(III)-dicitrate

0.0168 - 0.5762

Fe(III)-EDTA

6 entries

0.0004 - 0.0078

Fe(III)-enterobactin

0.0014

Fe(III)-vibriobactin

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wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C

0.043

NADPH

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at pH 7.5 and 37°C

722534

0.24 - 1.11

Fe(III)-citrate

0.95 - 2

Fe(III)-EDTA

9.193

Fe(III)-(N-2,3-dihydroxybenzoyl-Gly-Thr)3

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wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C

0.091 - 23.35

Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3

0.02

Fe(III)-aerobactin

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wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C

1.59 - 41

Fe(III)-citrate

0.139 - 5.427

Fe(III)-dicitrate

0.12 - 122

Fe(III)-EDTA

0.032 - 8.211

Fe(III)-enterobactin

1.268

Fe(III)-vibriobactin

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wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C

0.001

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using ferric enterobactin as substrate, at pH 7.5 and 37°C

722534

0.0035

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with Fe(III)-cyclam as substrate, at pH 7.0 and 30°C

0.02

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crude extract, using tert-butyl hydroperoxide as substrate, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C

0.0204

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with Fe(III)-diethylenetriaminepentaacetate as substrate, at pH 7.0 and 30°C

0.022

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using Fe(III)-EDTA as substrate, at pH 7.5 and 37°C

722534

0.0294

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with Fe(III)-(citrate)2 as substrate, at pH 7.0 and 30°C

0.0333

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with Fe(III)-tris(2-pyridylmethyl)amine as substrate, at pH 7.0 and 30°C

0.0393

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with Fe(III)-EDTA as substrate, at pH 7.0 and 30°C

0.0408

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with Fe(III)-(1,10-phenanthroline)3 as substrate, at pH 7.0 and 30°C

0.0454

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with Fe(III)-(catechol)2 as substrate, at pH 7.0 and 30°C

0.047

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with Fe(III)-nitrilotriacetic acid as substrate, at pH 7.0 and 30°C

0.0907

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with Fe(III)-(L-DOPA)2 as substrate, at pH 7.0 and 30°C

0.91

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after 45.5fold purification, using tert-butyl hydroperoxide as substrate, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C

9.4

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calculated from amino acid sequence

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cultivar DK Exquisite

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cv. Ashley

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721185, 721629, 722534, 766327, 766329

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Escherichia coli MG1655

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-

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-

-

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cultivar Dongjin

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UniProt

Panicum sumatrense RLM-37

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UniProt

-

-

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detected in the infection zone and nitrogen-fixing region

chloroplast

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inner chloroplast envelope membrane

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5774

766807

Highest Expressing Human Cell Lines

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malfunction

metabolism

physiological function

5 entries

additional information

32400

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x * 32400, His-tagged enzyme, SDS-PAGE

32412

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x * 32412, His-tagged enzyme, calculated from amino acid sequence

34000

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2 * 34000, SDS-PAGE

60000

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gel filtration

80500

Lathyrus oleraceus

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x * 80500, calculated from amino acid sequence

81500

-

x * 81500, SDS-PAGE

?

homodimer

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2 * 34000, SDS-PAGE

additional information

hanging drop vapor diffusion method, using 0.1 M succinic acid-sodium phosphate dibasic-glycine buffer pH 4.0 and 18% (w/v) PEG 1500

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K55A

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the mutant shows strongly reduced activity compared to the wild type enzyme

R130A

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the mutant shows strongly reduced activity compared to the wild type enzyme

R246A

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the variant is rather unstable, showing precipitate formation and cofactor release during protein concentration

57.5

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melting temperature

ammonium sulfate precipitation, butyl Toyopearl column chromatography, DEAE Sepharose column chromatography, POROS HQ-H column chromatography, and Red Sepharose column chromatography

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HisTrap column chromatography

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766329, 766327

HiTrap DEAE column chromatography, Superdex 75 gel filtration

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native enzyme

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Ni-NTA-Sepharose column chromatography and DEAE Sepharose column chromatography

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Strep-Tactin column chromatography

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expressed in Escherichia coli BL21 cells

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expressed in Escherichia coli BL21(DE3) cells

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721185, 722534, 766329, 766327

expressed in Escherichia coli DH10B cells

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expressed in Escherichia coli strain JM109

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expressed in Phaseolus vulgaris leaf chloroplasts

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expressed in Saccharomyces cerevisiae strain BJ2168

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766807

expressed in Saccharomyces cerevisiae strain DY1457

Lathyrus oleraceus

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gene FRO2, DNA and amino acid sequence determination and analysis, sequence comparison, the predicted gene structure shows the presence of 6 exons and 5 introns and its protein sequence contains ferric reductase and NOX_Duox_Like_FAD_NADP domains

Panicum sumatrense

A0A1U9W5F0

743901

gene FRO2, sequence comparison, the predicted gene structure shows the presence of 6 exons and 5 introns and its protein sequence contains ferric reductase and NOX_Duox_Like_FAD_NADP domains

Oryza sativa

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743901

addition of Fe2+ decreases yqjH expression starting at 100 nM, with a maximum repression of 4fold occurring at 0.01 mM Fe2+. Addition of CoCl2 concentrations of more than 500 nM also repress yqjH expression by about 50%

enzyme transcript abundance is decreased under excess Fe conditions

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766807

FRO2 transcript shows abundance under iron deficiency. The addition of 300 mM bathocuproine disulfonic acid further reduces FRO2 transcript abundance in plants grown under high-iron conditions for 3 days

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iron deficiency does not increase isoform FRO7 expression

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the enzyme is expressed at high levels in iron-deficient roots

Lathyrus oleraceus

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YqjI-dependent transcriptional repression is reduced when cells are exposed to elevated nickel levels, resulting in increased expression of yqjH. Upon addition of NiCl2, yqjH expression increases starting at 100 nM NiCl2, with maximum induction by 0.001 mM NiCl2 (42%). Deletion of yqjI leads to constitutive expression levels of yqjH that are 30fold higher than wild type basal expression levels