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IUBMB Comments Isolated from the bacterium Streptomyces melanosporofaciens M1614-43f2. Involved in the biosynthesis of cyclooctatin.
Synonyms
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CotB3
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cyclooctat-9-en-7-ol + reduced acceptor + O2 = cyclooctat-9-ene-5,7-diol + acceptor + H2O
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MetaCyc
cyclooctatin biosynthesis
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cyclooctat-9-en-7-ol,hydrogen-donor:oxygen oxidoreductase (5-hydroxylating)
Isolated from the bacterium Streptomyces melanosporofaciens M1614-43f2. Involved in the biosynthesis of cyclooctatin.
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cyclooctat-9-en-7-ol + reduced acceptor + O2
cyclooctat-9-ene-5,7-diol + acceptor + H2O
cyclooctat-9-en-7-ol + reduced acceptor + O2
cyclooctat-9-ene-5,7-diol + acceptor + H2O
Substrates: the enzyme is involved in the biosynthesis of cyclooctatin
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cyclooctat-9-en-7-ol + reduced acceptor + O2
cyclooctat-9-ene-5,7-diol + acceptor + H2O
Substrates: -
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cyclooctat-9-en-7-ol + reduced acceptor + O2
cyclooctat-9-ene-5,7-diol + acceptor + H2O
Substrates: redox partner promiscuity of CotB3. The reductase/ferredoxin system from Streptomyces afghaniensis (AfR/Afx) is superior in activating the cylcooctatin-specific class I P450s CotB3/CotB4 compared to the conventional Pseudomonas putida reductase/ferredoxin system (PdR/Pdx)
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cyclooctat-9-en-7-ol + reduced acceptor + O2
cyclooctat-9-ene-5,7-diol + acceptor + H2O
Substrates: the enzyme is involved in the biosynthesis of cyclooctatin
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cyclooctat-9-en-7-ol + reduced acceptor + O2
cyclooctat-9-ene-5,7-diol + acceptor + H2O
Substrates: -
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cyclooctat-9-en-7-ol + reduced acceptor + O2
cyclooctat-9-ene-5,7-diol + acceptor + H2O
cyclooctat-9-en-7-ol + reduced acceptor + O2
cyclooctat-9-ene-5,7-diol + acceptor + H2O
Substrates: the enzyme is involved in the biosynthesis of cyclooctatin
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cyclooctat-9-en-7-ol + reduced acceptor + O2
cyclooctat-9-ene-5,7-diol + acceptor + H2O
Substrates: the enzyme is involved in the biosynthesis of cyclooctatin
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UniProt
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UniProt
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Highest Expressing Human Cell Lines
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Gene Links
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metabolism
the enzyme is involved in the biosynthesis of cyclooctatin
metabolism
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the enzyme is involved in the biosynthesis of cyclooctatin
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COTB3_STRMJ
459
0
50687
Swiss-Prot
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expression in Escherichia coli BL21(DE3)
overexpression of the cyclooctatin biosynthetic gene cluster in Escherichia coli
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synthesis
efficient heterologous reconstitution of the cyclooctatin biosynthesis cluster by introduction of a novel, non-native Streptomyces-derived redox system (reductase/ferredoxin system from Streptomyces afghaniensis). The complete, heterologous biosynthesis cascade is established in Escherichia coli. The microbial production system allows for a 43fold increased cyclooctatin yield compared to the native producer Streptomyces melanosporofaciens
synthesis
CotB3 can be employed to functionalize other diterpene olefins, such as (-)-casbene to yield sinularcasbane D
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Kim, S.; Zhao, P.; Igarashi, M.; Sawa, R.; Tomita, T.; Nishiyama, M.; Kuzuyama, T.
Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster affordxa0a diterpene cyclase and two P450 hydroxylases
Chem. Biol.
16
736-743
2009
Streptomyces melanosporofaciens (C9K1X6), Streptomyces melanosporofaciens MI614-43F2 (C9K1X6)
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Grner, C.; Schrepfer, P.; Redai, V.; Wallrapp, F.; Loll, B.; Eisenreich, W.; Haslbeck, M.; Brck, T.
Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids
Microb. Cell Fact.
15
86
2016
Streptomyces melanosporofaciens (C9K1X6)
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