The enzyme participates in the Rut pyrimidine catabolic pathway. The flavin-N5-oxide that is formed by the enzyme reacts spontaneously with NADH to give oxidized flavin, releasing a water molecule.
The enzyme appears in viruses and cellular organisms
The enzyme participates in the Rut pyrimidine catabolic pathway. The flavin-N5-oxide that is formed by the enzyme reacts spontaneously with NADH to give oxidized flavin, releasing a water molecule.
Substrates: RutA directly cleaves the uracil ring between N-3 and C-4 to yield ureidoacrylate Products: product analysis by both NMR spectroscopy and mass spectrometry, overview
Substrates: the pyrimidine ring is cleaved directly by adding a pair of oxygen atoms and producing ureidoacrylate peracid. This odd metabolite, a strong and highly toxic oxidizing agent, is reduced spontaneously, albeit slowly, to ureidoacrylate Products: -
Substrates: (Z)-3-ureidoacrylic peracid decomposes nonenzymatically to form primarily (Z)-3-ureidoacrylic acid and uracil, half life of (Z)-3-ureidoacrylic peracid is 3 hours at pH 10.0 at 25°C and much less at lower pH Products: -
Substrates: the pyrimidine ring is cleaved directly by adding a pair of oxygen atoms and producing ureidoacrylate peracid. This odd metabolite, a strong and highly toxic oxidizing agent, is reduced spontaneously, albeit slowly, to ureidoacrylate Products: -
RutA has a central role in the Rut pathway, which is composed of seven proteins, all of which are required by Escherichia coli K-12 to grow on uracil as the sole nitrogen source
the Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37°C, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature, RutE/YdfG function. The catabolic pathway is highly expressed even in the absence of exogenous substrate and at 37°C
RutA is involved in the pyrimidine catabolic Rut pathway, which converts uracil to 3-hydroxypropionate or 2-methyl-3-hydroxypropionate. The flavoenzyme RutA, along with the flavin reductase RutF, catalyzes the initial uracil ring opening by an unprecedented oxidative hydrolysis reaction to give 3-ureidoacrylate
RutA has a central role in the Rut pathway, a pyrimidine catabolic pathway, which is composed of seven proteins, all of which are required by Escherichia coli K-12 to grow on uracil as the sole nitrogen source, pathway overview. RutA works in conjunction with the flavin reductase RutF
RutA is the first enzyme of the pyrimidine catabolizing Rut pathway, the involved enzymes are encoded by the rut operon, including rutRABCDEFG and ydfG, overview. RutF functions as a flavin reductase that is required to regenerate the flavin mononucleotide, FMN, cofactor
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