Information on EC 1.14.99.22 - ecdysone 20-monooxygenase

285451, 285454, 285457, 285462

Substrates: -
Products: -

alpha-ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Q2EMR3

Substrates: -
Products: -

alpha-ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Parasarcophaga argyrostoma

Substrates: -
Products: -

alpha-ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

alpha-ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

alpha-ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

alpha-ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Ajuga reptans

657574

Substrates: -
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: -
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: -
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: -
Products: -

ecdysone + NADPH + H+ + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + H+ + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + H+ + O2

20-hydroxyecdysone + NADP+ + H2O

684740, 715821

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

285460, 285461

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Q3LFR2

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: alpha-ecdysone, a secretory product of prothoracic glands
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

285454, 285457

Substrates: last step in biosynthesis of insect molting hormone
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: midgut microsomes show higher specific activity with 2fold higher Vmax, while total activity is 8-10fold higher in mitochondria
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Parasarcophaga argyrostoma

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

additional information

Substrates: recombinant GST-fusion protein, expressed in E. coli, is also able to transfer electrons from NADPH to a microsomal steroid hydroxylase p-450 and support steroidogenesis
Products: -

additional information

Substrates: E20-M preferentially uses NADPH in catalyzing ecdysone conversion to the physiologically active molting hormone, 20-hydroxyecdysone
Products: -

show all columns Go to Natural Substrate Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

7 entries

ecdysone + NADPH + H+ + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

18 entries

ecdysone + reduced acceptor + O2

20-hydroxyecdysone + acceptor + H2O

Substrates: -
Products: -

additional information

Substrates: E20-M preferentially uses NADPH in catalyzing ecdysone conversion to the physiologically active molting hormone, 20-hydroxyecdysone
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Ajuga reptans

657574

Substrates: -
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: -
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: -
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone
Products: -

ecdysone + AH2 + O2

20-hydroxyecdysone + A + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

285460, 285461

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Q3LFR2

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: alpha-ecdysone, a secretory product of prothoracic glands
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

285454, 285457

Substrates: last step in biosynthesis of insect molting hormone
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: midgut microsomes show higher specific activity with 2fold higher Vmax, while total activity is 8-10fold higher in mitochondria
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Parasarcophaga argyrostoma

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: -
Products: -

ecdysone + NADPH + O2

20-hydroxyecdysone + NADP+ + H2O

Substrates: serves as precursor of ecdysterone, the active moulting hormone in larval insects
Products: -

show all columns Go to Cofactor Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

cytochrome P450

15 entries

Ferredoxin

heme

NADH

NADPH

cytochrome P450

essential

cytochrome P450

essential

cytochrome P450

essential

285452, 285459

cytochrome P450

essential

cytochrome P450

essential

cytochrome P450

essential

cytochrome P450

essential

cytochrome P450

Parasarcophaga argyrostoma

essential

cytochrome P450

essential

285460, 285461

cytochrome P450

a cytochrome P450-dependent hydroxylase

cytochrome P450

a cytochrome P450-dependent hydroxylase

cytochrome P450

a cytochrome P450-dependent hydroxylase

cytochrome P450

cytochrome P450

cytochrome P450

Ferredoxin

isozyme in mitochondria

Ferredoxin

isozyme in mitochondria

Ferredoxin

isozyme in mitochondria

Ferredoxin

Parasarcophaga argyrostoma

isozyme in mitochondria

NADH

increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor

NADH

increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor

NADPH

NADPH

NADPH

NADPH

NADPH

NADPH

dependent on

NADPH

Parasarcophaga argyrostoma

dependent on

NADPH

ultimate hydrogen donor, cannot be substituted by NADH or ascorbate

NADPH

ultimate hydrogen donor, cannot be substituted by NADH or ascorbate

NADPH

NADP+ is not effective together with either malate, isocitrate, succinate, but with glucose-6-phosphate 25% of the activity is restored

NADPH

NADPH

NADPH

NADPH

NADPH

show all columns Go to Metals and Ions Search

NADPH

preferred cofactor

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Fe2+

Fe2+

a cytochrome P450-dependent hydroxylase

Fe2+

a cytochrome P450-dependent hydroxylase

Fe2+

a cytochrome P450-dependent hydroxylase

show all columns Go to Inhibitor Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1,4-anthraquinone

about 20% residual activity at 10 mM

1,9-Dideoxyforskolin

1H-[1,2,4]oxadizolo[4,3-a]-quinoxalin-1-one

a guanylate cyclase inhibitor, applied with ecdysone agonist RH-5849 results in 63% reduced E20M activity

2-isopropoxy-1,4-naphthoquinone

2-methoxy-1,4-anthraquinone

about 18% residual activity at 1 mM

2-methoxy-1,4-naphthoquinone

2-propoxy-1,4-naphthoquinone

20-hydroxyecdysone

6-anilinoquinoline-5,6-quinone

LY-38583, a guanylate cyclase inhibitor, applied with ecdysone agonist RH-5849 results in 80% reduced E20M activity, addition of guanylate cyclase activator S levels the activity to 78% of control activity

7,8-Benzoflavone

7-O-Hemisuccinyl-7-deacetyl-forskolin

9-hydroxy-1,4-anthraquinone

about 40% residual activity at 10 mM

9-methoxy-1,4-anthraquinone

about 45% residual activity at 10 mM

Ca2+

cytochrome c

diquat dibromide

ecdysterone

competitive inhibition

EDTA

forskolin

KCN

25% inhibition

KK-42

i.e. 1-benzyl-5[(E)-2,6-dimethyl-1,5-heptadienyl]imidazole

methylene blue

a guanylate cyclase inhibitor, applied with ecdysone agonist RH-5849 results in 86% reduced E20M activity, reversed by addition of dibutyryl-cGMP/theophylline to 87% of control activity

Metyrapone

Mg2+

NADP+

competitive inhibition

NEM

nicotinamide

Non-ionic detergents

Tween 20, Tween 80 and Triton X-100

oxidized cytochrome c

reversed by KCN

PCMB

Phospholipase A

Phospholipase C

Piperonylbutoxide

quinizarin

about 60% residual activity at 10 mM

SKF 525A

Tris/HCl-buffer

Tween 80

additional information

1,9-Dideoxyforskolin

dose dependent inhibition

1,9-Dideoxyforskolin

dose dependent inhibition

2-isopropoxy-1,4-naphthoquinone

synthetic compound

2-isopropoxy-1,4-naphthoquinone

synthetic compound

2-isopropoxy-1,4-naphthoquinone

synthetic compound

2-methoxy-1,4-naphthoquinone

isolated from the leaves of Impatiens glandulifera

2-methoxy-1,4-naphthoquinone

isolated from the leaves of Impatiens glandulifera

2-methoxy-1,4-naphthoquinone

isolated from the leaves of Impatiens glandulifera

2-propoxy-1,4-naphthoquinone

synthetic compound

2-propoxy-1,4-naphthoquinone

synthetic compound

2-propoxy-1,4-naphthoquinone

synthetic compound

20-hydroxyecdysone

competitive inhibition

20-hydroxyecdysone

competitive inhibition

7-O-Hemisuccinyl-7-deacetyl-forskolin

7-O-Hemisuccinyl-7-deacetyl-forskolin

partially reversible by irradiation with monochromatic light

partially reversible by irradiation with monochromatic light

partially reversible by irradiation with monochromatic light

diquat dibromide

Parasarcophaga argyrostoma

diquat dibromide

forskolin

7beta-acetoxy-8,13-epoxy-1alpha,6beta,9alpha-dihydroxylabd-14-en-11-one, adenylate cyclase activator

forskolin

7beta-acetoxy-8,13-epoxy-1alpha,6beta,9alpha-dihydroxylabd-14-en-11-one, adenylate cyclase activator

Metyrapone

Metyrapone

Metyrapone

2-methyl-1,2-di-3'-pyridylpropan-1-one, up to 80% inhibition

Metyrapone

Tween 80

Parasarcophaga argyrostoma

microsomal isozyme

Tween 80

microsomal isozyme

additional information

inhibition profiles

additional information

inhibition profiles

additional information

inhibition profiles

additional information

inhibitory effects of guanylate cyclase inhibitors with RH-5849 in vivo, overview

additional information

2-hydroxy-1,4-anthraquinone is essentially without effect on enzyme activity

additional information

in pure N2 atmosphere, enzyme activity is 50% inhibited due to missing oxygen

show all columns Go to Activating Compound Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

20-hydroxyecdysone

injection of 20-hydroxyecdysone stimulates ecdysone 20-hydroxylase activity in the midgut

9-methoxy-1,4-anthraquinone

about 125% activity at 0.001 mM

ATP

increase of activity

dibutyryl-cGMP

activates E20M activity by 50fold, diminished by injections of guanylate cyclase inhibitors alone or combined with ecdysone agonist RH-5849

Juvenile hormone

L-dihydroxyphenylalanine

octopamine

additional information

octopamine

octopamine

additional information

heat stress induces activity of ecdysone 20-monooxygenase in Drosophila females and enzyme activity correlates with the level of 20-hydroxyecdysone

additional information

no activation by dibutyryl cAMP, cGMP, or cAMP

show all columns Go to KM Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.00125

alpha-ecdysone

0.000000367 - 0.025

ecdysone

0.0105

NADPH

0.000000367

ecdysone

microsomal enzyme

0.0000163

ecdysone

mitochondrial enzyme

0.00027

ecdysone

0.00071

ecdysone

0.00183

ecdysone

mitochondrial enzyme system

0.025

ecdysone

show all columns Go to IC50 Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

IC50 VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.3

1,4-anthraquinone

Manduca sexta

at pH 7.5 and 35°C

0.04

2-methoxy-1,4-anthraquinone

Manduca sexta

at pH 7.5 and 35°C

9-hydroxy-1,4-anthraquinone

Manduca sexta

at pH 7.5 and 35°C

9-methoxy-1,4-anthraquinone

Manduca sexta

at pH 7.5 and 35°C

quinizarin

Manduca sexta

at pH 7.5 and 35°C

show all columns Go to Specific Activity Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.00000000617

Parasarcophaga argyrostoma

fat body

0.0000000103

fat body

0.00000017

fat body

0.00000041

mid- and hindgut

0.00000075

microsomes, fat body

0.00000115

malphigian tubules

0.000003

microsomes, malphigian tubules

0.00000433

0.00000562

malphigian tubules, 10000 x g pellet

0.0000183

0.0000197

fat body

0.0000466

mitochondrial enzyme system

0.0001721

midgut

0.0021

microsomes

recombinant GST-fusion protein, purified

additional information

additional information

additional information

additional information

wild-type and mutant enzyme activities at different temperatures, overview

additional information

additional information

additional information

show all columns Go to pH Optimum Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

6.5

6.6

6.8 - 8

potassium phosphate buffer

7.2

7.5

6.5

6.5

6.5

7.2

Parasarcophaga argyrostoma

microsomal isozyme

7.2

microsomal isozyme

7.5

assay at

7.5

7.5

assay at

7.5

show all columns Go to pH Range Search

activity assay

7.5

activity assay

7.5

assay at

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

6 - 8.5

show all columns Go to Temperature Optimum Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Parasarcophaga argyrostoma

microsomal isozyme

microsomal isozyme

activity assay

activity assay

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

females

brenda

brenda

canton S strain

brenda

crayfish

brenda

brenda

Colorado potato beetle

UniProt

brenda

Parasarcophaga argyrostoma

flesh-fly

brenda

flesh-fly

brenda

brenda

cotton leafworm

brenda

UniProt

brenda

brenda

silkworm

brenda

SwissProt

brenda

brenda

canton S strain

brenda

wild-type strain Canton S, octopamineless strain Tbetah nM18

brenda

brenda

wild-type strain 101

brenda

L. migratoria migratorioides: african migration locust

brenda

migration locust

brenda

SwissProt

brenda

684740, 714771, 715821

brenda

enzyme expression correlates with the hemolymph ecdosteroid titre during larval and pupal development

671944

brenda

tobacco hornworm

285451, 285454, 285457, 285462, 745123

brenda

desert locust

brenda

gene SgShd

UniProt

brenda

show all columns Go to Source Tissue Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

brenda

brenda

brenda

brenda

brenda

egg

brenda

brenda

source of steroid hormone production

brenda

brenda

brenda

additional information

body wall

Parasarcophaga argyrostoma

brenda

brenda

egg

Q3LFR2

nondiapause egg

brenda

egg

brenda

epidermis

brenda

brenda

brenda

larval

brenda

brenda

larval

brenda

larval

brenda

larval

brenda

enzyme is located in mitochondrion

brenda

three successive peaks of expression during the fifth instar and the beginning of larval development

brenda

from fifth instar larvae

brenda

brenda

Parasarcophaga argyrostoma

third instar wandering stage larvae 67-68% of total activity, after white prepuparial stage activity is inactivated in fat body

brenda

third instar wandering stage larvae 67-68% of total activity, after white prepuparial stage activity is inactivated in fat body

brenda

larval

brenda

brenda

brenda

wandering stage third instar larvae

brenda

larva

brenda

fifth instar larvae

brenda

during the last larval stadium of the midgut, E20M activity increases 50fold coincident with wandering onset

brenda

brenda

highest activity during vegetation stage, i.e. May

brenda

brenda

brenda

malpighian tubule

brenda

malpighian tubule

last instar larvae

brenda

brenda

three successive peaks of expression during the fifth instar and the beginning of larval development

brenda

malpighian tubule

Parasarcophaga argyrostoma

brenda

brenda

brenda

brenda

brenda

brenda

larval, enzyme is located in mitochondrion and microsome

brenda

larval

brenda

three successive peaks of expression during the fifth instar and the beginning of larval development

brenda

from fifth instar larvae

brenda

during the last larval stadium of the midgut, E20M activity increases 50fold coincident with wandering onset

brenda

during the ten-day developmental period preceding the larval-pupal molt, fifth larval instar, onset of wandering behavior and a 50fold increase in ecdysone 20-monooxygenase activity occur

brenda

fifth larval instar midgut

brenda

pupa

in the pupa of Bombyx mori, the rectal sac is an essential organ that operates as a repository for degraded ecdysteroids, and it can be distended by administration of 20-hydroxyecdysone. rectal sac distention appears 4 days after 20-hydroxyecdysone administration

brenda

pupa

brenda

ring gland

Parasarcophaga argyrostoma

brenda

brenda

Parasarcophaga argyrostoma

8-9% of total activity

brenda

trachea

8-9% of total activity

brenda

additional information

enzyme activity measured in diapause and non-diapause egg subcellular preparations

brenda

additional information

immunological analysis of enzyme content in diapause and non-diapause egg microsomes

brenda

additional information

investigation of enzyme activity in fat body and midgut gland during developmental stages from egg to adult

brenda

additional information

expression in tissues peripheral to the prothoracic glands mainly during the fifth instar

671944

brenda

show all columns Go to Localization Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

brenda

brenda

microsome

bound to sedimentable membrane

brenda

brenda

Parasarcophaga argyrostoma

microsomal isozyme

brenda

microsomal isozyme

brenda

brenda

brenda

brenda

Parasarcophaga argyrostoma

mitochondrial isozyme

brenda

mitochondrial isozyme

5739

brenda

mitochondrion

membrane bound

5739

brenda

mitochondrion

membrane bound

5739

brenda

top hide

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

show all columns Go to General Information Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

evolution

Gecarcinus lateralis

presence of six Shed orthologs in the Gecarcinus lateralis Y-organ transcriptome, which are assigned to classes Shed1, 2, 4A, 4B, 5A, and 5B

766849

malfunction

physiological function

additional information

malfunction

RNAi-mediated knockdown of Shade negatively affects ecdysone-20-hydroxylation in the desert locust

malfunction

enzyme knockdown causes larval lethality, delayed development and affects pupation

physiological function

essential need for conversion of the molting hormone, ecdysone, to its physiologically active form, 20-hydroxyecdysone by E20-M during the post-embryonic development

physiological function

in the desert locust, the Halloween gene SgShd plays a role in the biosynthesis of 20-hydroxyecdysone

additional information

physiological and developmental significance of the mitochondrial, NADPH-forming energy-linked transhydrogenations, that catalyzes a reversible hydride ion transfer between NADP(H) and NAD(H), for E20-M activity. NADPH-forming activity occurs as a nonenergy- or energy-linked activity with energy for the latter derived from either electron transport-dependent NADH or succinate utilization, or ATP hydrolysis by Mg2+-dependent ATPase, overview

additional information

in the pupa of the silkworm, Bombyx mori, the rectal sac is an essential organ that operates as a repository for degraded ecdysteroids, and it can be distended by administration of 20-hydroxyecdysone

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

Q9VUF8 pBLAST

CP314_DROME

540

61690

Swiss-Prot

H9MFM9 pBLAST

H9MFM9_SCHGR

224

25225

TrEMBL

Q2EMR3 pBLAST

Q2EMR3_MANSE

516

58237

TrEMBL

other Location (Reliability: 2)

Q3LFR2 pBLAST

Q3LFR2_BOMMO

516

58515

TrEMBL

V5K5L7 pBLAST

V5K5L7_LEPDE

473

54415

TrEMBL

show all columns Go to Molecular Weight Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

103000

? * 103000, SDS-PAGE, recombinant GST-fusion protein

60000

? * 60000, SDS-PAGE

77700

? * 77700, sequence determination

show all columns Go to Subunit Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

? * 77700, sequence determination

? * 103000, SDS-PAGE, recombinant GST-fusion protein

? * 60000, SDS-PAGE

show all columns Go to Protein Variants Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

additional information

additional information

wild type and mutant strain females differ by the mode of heat stress response of ecdysone and 20-hydroxyecdysone at 38°C

additional information

RNAi knockdown of gene SgShd, mutant phenotype in the fifth larval stage, temporal profiling of fifth larval stage, overview

Go to General Stability Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

bovine serum albumin stabilizes

instable, linear activity under standard assay conditions for 10 min

Go to Storage Stability Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-30°C, microsomal preparations with 30% v/v glycerol

-80°C, quick frozen membranes, 15% glycerol v/v, at least 1 month

4°C to -20°C storage for prolonged periods not possible due to unstability of the enzyme, appreciable loss of activity

Go to Purification (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

not: purification of the enzyme from microsomes failed due to unstability of the enzyme

recombinant GST-fusion protein

solubilization of the enzyme from microsomes with detergent Synperonic NP10

Go to Cloned (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

cDNA isolated from non-diapause eggs, expression in Escherichia coli BL21 as GST-fusion protein

cloned CYP6H1 cDNA is probably identical with purified enzyme

expressed in Sf9 insect cells

expression in S2 cells

gene SgShd, DNA and amino acid sequence determination and analysis

into a pSTBlue-1 Acceptor vector for sequencing, recombinant baculovirus harboring the BmE20OHase cDNA is constructed using the BAC-TO-BAC baculovirus expression system

Go to Expression Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

EXPRESSION

ORGANISM

UNIPROT

LITERATURE

ecdysone 20-hydroxylase expression is not induced by 20-hydroxyecdysone

L-dihydroxyphenylalanine and octopamine feeding increases ecdysone 20-monooxygenase activity by a factor of 1.6 and 1.7, respectively

top print hide References Search

mRNA levels of the six Shed genes in the Y-organ are comparable to those in nine other tissues that include hepatopancreas and muscle. Molt stage has little effect on Shed1 and Shed5B expression in the Y-organ. Shed5A is expressed at the highest mRNA levels in the Y-organ and is significantly increased during early and mid premolt stages. SB431542 inhibits transforming growth factor-beta/activin signaling and blocks Y-organ commitment, also decreases Shed2 and Shed4A mRNA levels at 14 days post-eyestalk ablation

Gecarcinus lateralis

766849

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

Johnson, P.; Rees, H.H

The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria

Biochem. J.

168

513-520

1977

Schistocerca gregaria

606249

brenda

Keogh, D.P.; Mitchell, M.J.; Crooks, J.R.; Smith, S.L.

Effects of the adenylate cyclase activator forskolin and its inactive derivative 1,9-dideoxyforskolin on insect cytochrome P-450 dependent steroid hydroxylase activity

Experientia

39-41

1991

Drosophila melanogaster, Drosophila melanogaster Canton S, Manduca sexta

1737575

brenda

Feyereisen, R.; Durst, F.

Ecdysterone biosynthesis: a microsomal cytochrome-P-450-linked ecdysone 20-monooxygenase from tissues of the African migratory locust

Eur. J. Biochem.

37-47

1978

Locusta migratoria migratorioides

27363

brenda

Greenwood, D.R.; Rees, H.H.

Ecdysone 20-mono-oxygenase in the desert locust, Schistocerca gregaria

Biochem. J.

223

837-847

1984

Schistocerca gregaria

6439188

brenda

Weirich, G.F.

Ecdysone 20-monooxygenase

Methods Enzymol.

454-458

1985

Manduca sexta

brenda

Grebenok, R.J.; Galbraith, D.W.; Benveniste, I.; Feyereisen, R.

Ecdysone 20-monooxygenase , a cytochrome P-450 enzyme from spinach, Spinacia oleracea

Phytochemistry

927-933

1996

Spinacia oleracea

brenda

Shergill, J.K.; Cammack, R.; Chen, J.H.; Fisher, M.J.; Madden, S.; Rees, H.H.

EPR spectroscopic characterization of the iron-sulphur proteins and cytochrome P-450 in mitochondria from the insect Spodoptera littoralis (cotton leafworm)

Biochem. J.

307

719-728

1995

Spodoptera littoralis

brenda

Weirich, G.F.; Williams, V.P.; Feldlaufer, M.F.

Ecdysone 20-hydroxylation in Manduca sexta midgut: kinetic parameters of mitochondrial and microsomal ecdysone 20-monooxygenases

Arch. Insect Biochem. Physiol.

305-312

1996

Manduca sexta

brenda

Darvas, B.; Rees, H.H.; Hoggard, N.

Ecdysone 20-monooxygenase systems in flesh-flies (diptera: sarcophagidae), Neobellieria bullata and Parasarcophaga argyrostoma

Comp. Biochem. Physiol. B

105

765-773

1993

Sarcophaga bullata, Parasarcophaga argyrostoma

brenda

Winter, J.; Eckerskorn, C.; Waditschatka, R.; Kayser, H.

A microsomal ecdysone-binding cytochrome P-450 from the insect Locusta migratoria purified by sequential use of type-II and type-I ligands

Biol. Chem.

382

1541-1549

2001

Locusta migratoria migratorioides

11767943

brenda

Horike, N.; Sonobe, H.

Ecdysone 20-monooxygenase in eggs of the silkworm, Bombyx mori: enzymatic properties and developmental changes

Arch. Insect Biochem. Physiol.

9-17

1999

Bombyx mori

10331236

brenda

Horike, N.; Takemori, H.; Nonaka, Y.; Sonobe, H.; Okamoto, M.

Molecular cloning of NADPH-cytochrome P-450 oxidoreductase from silkworm eggs

Eur. J. Biochem.

267

6914-6920

2000

Bombyx mori

11082204

brenda

Mitchell, M.J.; Crooks, J.R.; Keogh, D.P.; Smith, S.L.

Ecdysone 20-monooxygenase activity during larval-pupal-adult development of the tabacco hornworm, Manduca sexta

Arch. Insect Biochem. Physiol.

24-32

1999

Manduca sexta

brenda

285463

Boecking, D.; Dauphin-Villemant, C.; Lafont, R.

Metabolism of 3-dehydroecdysone in the crayfish Orconectes limosus

Eur. J. Entomol.

63-74

1995

Faxonius limosus

brenda

657574

Alekseeva, L.I.

Ecdysone 20-monooxygenase activity of cytochrome P450 in Ajuga reptans L. plants and cell culture

Appl. Biochem. Microbiol.

135-139

2004

Ajuga reptans

brenda

Petryk, A.; Warren, J.T.; Marques, G.; Jarcho, M.P.; Gilbert, L.I.; Kahler, J.; Parvy, J.P.; Li, Y.; Dauphin-Villemant, C.; O'Connor, M.B.

Shade is the Drosophila P450 enzyme that mediates the hydroxylation of ecdysone to the steroid insect molting hormone 20-hydroxyecdysone

Proc. Natl. Acad. Sci. USA

100

13773-13778

2003

Drosophila melanogaster (Q9VUF8)

14610274

brenda

671944

Rewitz, K.F.; Rybczynski, R.; Warren, J.T.; Gilbert, L.I.

The Halloween genes code for cytochrome P450 enzymes mediating synthesis of the insect moulting hormone

Biochem. Soc. Trans.

1256-1260

2006

Manduca sexta

17073797

brenda

Rewitz, K.F.; Rybczynski, R.; Warren, J.T.; Gilbert, L.I.

Developmental expression of Manduca shade, the P450 mediating the final step in molting hormone synthesis

Mol. Cell. Endocrinol.

247

166-174

2006

Manduca sexta (Q2EMR3), Manduca sexta

16473459

brenda

Mitchell, M.J.; Brescia, A.I.; Smith, S.L.; Morgan, E.D.

Effects of the compounds 2-methoxynaphthoquinone, 2-propoxynaphthoquinone, and 2-isopropoxynaphthoquinone on ecdysone 20-monooxygenase activity

Arch. Insect Biochem. Physiol.

45-52

2007

Aedes aegypti, Drosophila melanogaster, Manduca sexta

17694563

brenda

Chentsova, N.A.; Gruntenko, N.E.; Rauschenbach, I.Y.

Ecdysone 20-monooxygenase activity in Drosophila virilis strains varying in ecdysteroid response to heat stress

Russ. J. Genet.

829-830

2007

Drosophila virilis

brenda

Maeda, S.; Nakashima, A.; Yamada, R.; Hara, N.; Fujimoto, Y.; Ito, Y.; Sonobe, H.

Molecular cloning of ecdysone 20-hydroxylase and expression pattern of the enzyme during embryonic development of silkworm Bombyx mori

Comp. Biochem. Physiol. B

149

507-516

2008

Bombyx mori (Q3LFR2), Bombyx mori

18226571

brenda

Rauschenbach, I.; Gruntenko, N.; Chentsova, N.; Adonyeva, N.; Alekseev, A.

Role of ecdysone 20-monooxygenase in regulation of 20-hydroxyecdysone levels by juvenile hormone and biogenic amines in Drosophila

J. Comp. Physiol. B

178

27-32

2008

Drosophila melanogaster, Drosophila virilis

17703313

brenda

Drummond, C.A.; Vandock, K.P.; Smith, S.L.; Fioravanti, C.F.

Cyclic 3',5' guanosine monophosphate and larval midgut ecdysone 20-monooxygenase activity of the tobacco hornworm, Manduca sexta

Comp. Biochem. Physiol. B

157

33-38

2010

Manduca sexta

20451652

brenda

Suzuki, T.; Sakurai, S.; Iwami, M.

Physiological requirements for 20-hydroxyecdysone-induced rectal sac distention in the pupa of the silkworm, Bombyx mori

J. Insect Physiol.

673-677

2010

Bombyx mori

20193691

brenda

Vandock, K.P.; Drummond, C.A.; Smith, S.L.; Fioravanti, C.F.

Midgut and fatbody mitochondrial transhydrogenase activities during larval-pupal development of the tobacco hornworm, Manduca sexta

J. Insect Physiol.

774-779

2010

Manduca sexta

20138054

brenda

Marchal, E.; Verlinden, H.; Badisco, L.; Van Wielendaele, P.; Vanden Broeck, J.

RNAi-mediated knockdown of Shade negatively affects ecdysone-20-hydroxylation in the desert locust, Schistocerca gregaria

J. Insect Physiol.

890-896

2012

Schistocerca gregaria (H9MFM9)

22465741

brenda

Kong, Y.; Liu, X.P.; Wan, P.J.; Shi, X.Q.; Guo, W.C.; Li, G.Q.

The P450 enzyme Shade mediates the hydroxylation of ecdysone to 20-hydroxyecdysone in the Colorado potato beetle, Leptinotarsa decemlineata

Insect Mol. Biol.

632-643

2014

Leptinotarsa decemlineata (V5K5L7), Leptinotarsa decemlineata

24989229

brenda