We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 1.14.19.55 - 4-hydroxybenzoate brominase (decarboxylating) for references in articles please use BRENDA:EC1.14.19.55
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Contains FAD. The enzyme, described from epiphytic marine bacteria of the genera Pseudoalteromonas and Marinomonas, is an unusual single-component FAD-dependent halogenase that contains a distinct NAD(P)H binding domain and does not require an additional flavin reductase for activity. The enzyme catalyses a bromination of its substrate, followed by a second bromination concurrent with decarboxylation.
The enzyme appears in viruses and cellular organisms
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
bmp5
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3,4-dihydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+ = 3,5-dibromobenzene-1,2-diol + 2 NADP+ + CO2 + 4 H2O
(2)
-
-
-
3,4-dihydroxybenzoate + NADPH + bromide + O2 + H+ = 3-bromo-4,5-dihydroxybenzoate + NADP+ + 2 H2O
(2a)
-
-
-
3-bromo-4,5-dihydroxybenzoate + NADPH + bromide + O2 + H+ = 3,5-dibromobenzene-1,2-diol + NADP+ + CO2 + 2 H2O
(2b)
-
-
-
3-bromo-4-hydroxybenzoate + NADPH + bromide + O2 + H+ = 2,4-dibromophenol + NADP+ + CO2 + 2 H2O
(1b)
-
-
-
4-hydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+ = 2,4-dibromophenol + 2 NADP+ + CO2 + 4 H2O
(1)
-
-
-
4-hydroxybenzoate + NADPH + bromide + O2 + H+ = 3-bromo-4-hydroxybenzoate + NADP+ + 2 H2O
(1a)
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-hydroxybenzoate:NADPH oxidoreductase (brominating, decarboxylating)
Contains FAD. The enzyme, described from epiphytic marine bacteria of the genera Pseudoalteromonas and Marinomonas, is an unusual single-component FAD-dependent halogenase that contains a distinct NAD(P)H binding domain and does not require an additional flavin reductase for activity. The enzyme catalyses a bromination of its substrate, followed by a second bromination concurrent with decarboxylation.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,4-dihydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
2,4-dibromo-6-hydroxyphenol + 4-bromo-2-hydroxyphenol + 2 NADP+ + CO2 + 4 H2O
3,4-dihydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
2,4-dibromo-6-hydroxyphenol + 4-bromo-2-hydroxyphenol + 2 NADP+ + CO2 + 4 H2O
3-bromo-4-hydroxybenzoate + NADPH + bromide + O2 + H+
2,4-dibromophenol + NADP+ + CO2 + 2 H2O
4-hydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
2,4-dibromophenol + 2 NADP+ + CO2 + 4 H2O
4-hydroxybenzoate + 2 NADPH + 2 iodide + 2 O2 + 2 H+
2,4-diiodophenol + 2 NADP+ + CO2 + 4 H2O
4-hydroxybenzoate + NADPH + bromide + O2 + H+
3-bromo-4-hydroxybenzoate + NADP+ + 2 H2O
additional information
?
-
2,4-dihydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
2,4-dibromo-6-hydroxyphenol + 4-bromo-2-hydroxyphenol + 2 NADP+ + CO2 + 4 H2O
-
-
-
-
?
2,4-dihydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
2,4-dibromo-6-hydroxyphenol + 4-bromo-2-hydroxyphenol + 2 NADP+ + CO2 + 4 H2O
-
-
-
-
?
3,4-dihydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
2,4-dibromo-6-hydroxyphenol + 4-bromo-2-hydroxyphenol + 2 NADP+ + CO2 + 4 H2O
-
-
-
-
?
3,4-dihydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
2,4-dibromo-6-hydroxyphenol + 4-bromo-2-hydroxyphenol + 2 NADP+ + CO2 + 4 H2O
-
-
-
-
?
3-bromo-4-hydroxybenzoate + NADPH + bromide + O2 + H+
2,4-dibromophenol + NADP+ + CO2 + 2 H2O
-
-
-
-
?
3-bromo-4-hydroxybenzoate + NADPH + bromide + O2 + H+
2,4-dibromophenol + NADP+ + CO2 + 2 H2O
-
-
-
-
?
4-hydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
2,4-dibromophenol + 2 NADP+ + CO2 + 4 H2O
-
-
-
-
?
4-hydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
2,4-dibromophenol + 2 NADP+ + CO2 + 4 H2O
-
-
-
-
?
4-hydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
2,4-dibromophenol + 2 NADP+ + CO2 + 4 H2O
-
-
-
-
?
4-hydroxybenzoate + 2 NADPH + 2 bromide + 2 O2 + 2 H+
2,4-dibromophenol + 2 NADP+ + CO2 + 4 H2O
-
-
-
-
?
4-hydroxybenzoate + 2 NADPH + 2 iodide + 2 O2 + 2 H+
2,4-diiodophenol + 2 NADP+ + CO2 + 4 H2O
-
-
-
-
?
4-hydroxybenzoate + 2 NADPH + 2 iodide + 2 O2 + 2 H+
2,4-diiodophenol + 2 NADP+ + CO2 + 4 H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + bromide + O2 + H+
3-bromo-4-hydroxybenzoate + NADP+ + 2 H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + bromide + O2 + H+
3-bromo-4-hydroxybenzoate + NADP+ + 2 H2O
-
-
-
-
?
additional information
?
-
-
no substrate: chloride
-
-
-
additional information
?
-
-
no substrate: chloride
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
FAD
-
Bmp5 does not require the addition of a flavin reductase to regenerate FADH2 in situ
NADPH
-
absolutely required
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
coexpression of Bmp5 with flavin-dependent 4-hydroxybenzoate-3-hydroxylase m3553 in Escherichia coli yields 2,4-dibromophenol, 4-bromo-2-hydroxyphenol and 2,4-dibromo-6-hydroxyphenol. Coexpression of Bmp5, Cyp450 enzyme Bmp7, and m3553, and supplementation of the culture medium with exogenous 4-hydroxybenzoic acid, leads to production of bromophenols, bromocatechols, mono- and dihydroxylated dibenzo-p-dioxins, mono- and dihydroxy-brominated diphenyl ethers, as well as biphenyls
physiological function
-
deletion of the Bmp5 gene eliminates the synthesis of bromophenol-containing species
physiological function
-
coexpression of Bmp5 with flavin-dependent 4-hydroxybenzoate-3-hydroxylase m3553 in Escherichia coli yields 2,4-dibromophenol, 4-bromo-2-hydroxyphenol and 2,4-dibromo-6-hydroxyphenol. Coexpression of Bmp5, Cyp450 enzyme Bmp7, and m3553, and supplementation of the culture medium with exogenous 4-hydroxybenzoic acid, leads to production of bromophenols, bromocatechols, mono- and dihydroxylated dibenzo-p-dioxins, mono- and dihydroxy-brominated diphenyl ethers, as well as biphenyls
-
physiological function
-
deletion of the Bmp5 gene eliminates the synthesis of bromophenol-containing species
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli
expression in Escherichia coli
-
expression in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Agarwal, V.; Moore, B.
Enzymatic synthesis of polybrominated dioxins from the marine environment
ACS Chem. Biol.
9
1980-1984
2014
Marinomonas mediterranea, Marinomonas mediterranea MMB-1
brenda
Agarwal, V.; El Gamal, A.; Yamanaka, K.; Poth, D.; Kersten, R.; Schorn, M.; Allen, E.; Moore, B.
Biosynthesis of polybrominated aromatic organic compounds by marine bacteria
Nat. Chem. Biol.
10
640-647
2014
Pseudoalteromonas luteoviolacea, Pseudoalteromonas luteoviolacea 2ta16
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
Enzyme Nomenclature
1.14.19.55 4-hydroxybenzoate brominase (decarboxylating)
top
Information
