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EC Tree
IUBMB Comments This enzyme is found in fungi and animals and catalyses a step in the biosynthesis of important sterol molecules such as ergosterol and cholesterol, respectively. The enzyme acts on the 4alpha-methyl group. Subsequent decarboxylation by EC 1.1.1.170, 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating), occurs concomitantly with epimerization of the remaining 4beta-methyl into the 4alpha position, thus making it a suitable substrate for a second round of catalysis. cf. EC 1.14.13.246, 4beta-methylsterol monooxygenase; EC 1.14.18.10, plant 4,4-dimethylsterol C-4alpha-methyl-monooxygenase; and EC 1.14.18.11, plant 4alpha-monomethylsterol monooxygenase.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
erg25, sc4mol, methyl sterol oxidase, erg25p, 4-methyl sterol oxidase, c-4 sterol methyl oxidase, c-4 methyl sterol oxidase, sterol c4 methyl oxidase, sterol-c4-methyl oxidase, 4,4-dimethyl-zymosterol 4alpha-methyl-oxidase,
more
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4,4-dimethyl-sterol 4alpha-methyl-oxidase
4,4-dimethyl-zymosterol 4alpha-methyl-oxidase
4-methyl sterol oxidase
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C-4 methyl sterol oxidase
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C-4 sterol methyl oxidase
EC 1.14.13.72
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formerly
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EC 1.14.99.16
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formerly
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methylsterol hydroxylase
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methylsterol monooxygenase
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NAD(P)H-dependent methylsterol oxidase
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sterol C-4 methyloxidase
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sterol C4 methyl oxidase
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4,4-dimethyl-sterol 4alpha-methyl-oxidase
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4,4-dimethyl-sterol 4alpha-methyl-oxidase
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4,4-dimethyl-zymosterol 4alpha-methyl-oxidase
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4,4-dimethyl-zymosterol 4alpha-methyl-oxidase
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4-methylsterol oxidase
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4-methylsterol oxidase
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C-4 sterol methyl oxidase
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C-4 sterol methyl oxidase
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ERG25
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ERG25p
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methyl sterol oxidase
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methyl sterol oxidase
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methyl sterol oxidase
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SMO
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sterol-C4-methyl oxidase
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sterol-C4-methyl oxidase
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sterol-C4-methyl-oxidase
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sterol-C4-methyl-oxidase
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3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + 2 ferrocytochrome b5 + O2 + 2 H+ = 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + 2 ferricytochrome b5 + H2O
(1c)
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ = 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + 2 ferricytochrome b5 + H2O
(1a)
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ = 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ = 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + 2 ferricytochrome b5 + 2 H2O
(1b)
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ = 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O
aerobic hydroxylation
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ = 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O
requires cytochrome b5. Also acts on 4alpha-methyl-5alpha-cholest-7-en-3beta-ol. the sterol can be based on cycloartenol as well as lanosterol. formerly EC 1.14.99.16
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ = 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O
overall reaction
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ = 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O
aerobic hydroxylation
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol,ferrocytochrome-b5:oxygen oxidoreductase (C4alpha-methyl-hydroxylating)
This enzyme is found in fungi and animals and catalyses a step in the biosynthesis of important sterol molecules such as ergosterol and cholesterol, respectively. The enzyme acts on the 4alpha-methyl group. Subsequent decarboxylation by EC 1.1.1.170, 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating), occurs concomitantly with epimerization of the remaining 4beta-methyl into the 4alpha position, thus making it a suitable substrate for a second round of catalysis. cf. EC 1.14.13.246, 4beta-methylsterol monooxygenase; EC 1.14.18.10, plant 4,4-dimethylsterol C-4alpha-methyl-monooxygenase; and EC 1.14.18.11, plant 4alpha-monomethylsterol monooxygenase.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + CO2
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + 3 reduced acceptor + 3 O2
(3beta,4alpha)-3-hydroxy-4-methylcholesta-8,14-24-triene-4-carboxylate + 3 acceptor + 3 H2O
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4,4-dimethyl-zymosterol + reduced acceptor + O2
4-hydroxymethyl,4-methyl-zymosterol + acceptor + H2O
4,4-dimethyl-zymosterol + reduced acceptor + O2
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artificial substrate
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4,4-[30,31-14C]dimethyl-5alpha-cholest-7-en-3beta-ol + ferrocytochrome b5 + O2 + H+
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4alpha-methyl-5alpha-cholest-7-en-3beta-ol + ferrocytochrome b5 + O2 + H+
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4alpha-methyl-5alpha-cholestan-3beta-ol + reduced acceptor
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4alpha[30-3H]-methyl-5alpha-cholest-7-en-3beta-ol + ferrocytochrome b5 + O2 + H+
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4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
additional information
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3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
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step 3 of 3 of the overall reaction
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3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
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step 3 of 3 of the overall reaction
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + CO2
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + CO2
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products of two step hydroxylase and demethylase activity, labile intermediate 4alpha-hydroxymethyl-4beta-methyl-5alpha-cholest-7-en-3beta-ol and 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + CO2
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
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step 1 of 3 of the overall reaction
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
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step 1 of 3 of the overall reaction
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4,4-dimethyl-zymosterol + reduced acceptor + O2
4-hydroxymethyl,4-methyl-zymosterol + acceptor + H2O
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4,4-dimethyl-zymosterol + reduced acceptor + O2
4-hydroxymethyl,4-methyl-zymosterol + acceptor + H2O
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a step in the ergosterol biosynthesis, pathway, overview
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4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
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step 2 of 3 of the overall reaction
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?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
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step 2 of 3 of the overall reaction
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additional information
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the enzyme is involved in cholesterol biosynthesis, (24S)-hydroxycholesterol downregulates cholesterol synthesis in neurons, possibly, in a posttranscriptional manner through SREBP-2, overview
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additional information
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squalene is sterol precursor, 24-methyl and 24-ethyl cholesterol are major sterol compounds in arbuscular mycorrhizal fungi, biosynthetic intermediates are lanosterol and 24-methylene lanosterol
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additional information
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squalene is sterol precursor, 24-methyl and 24-ethyl cholesterol are major sterol compounds in arbuscular mycorrhizal fungi, biosynthetic intermediates are lanosterol and 24-methylene lanosterol
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additional information
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enzyme is a key enzyme in ergosterol biosynthetic pathway in fungi, determination and analysis of sterol content in wild-type and mutant strains
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additional information
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enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview
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additional information
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substrate specificity, 24-methylene-24-dihydrocycloartenol is a poor substrate, no activity with 24-ethylidene-24-dihydrolophenol, overview
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additional information
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the enzyme is closely associated to Erg28p
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additional information
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enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview
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additional information
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substrate specificity, 24-methylene-24-dihydrocycloartenol is a poor substrate, no activity with 24-ethylidene-24-dihydrolophenol, overview
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3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
4,4-dimethyl-zymosterol + reduced acceptor + O2
4-hydroxymethyl,4-methyl-zymosterol + acceptor + H2O
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a step in the ergosterol biosynthesis, pathway, overview
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4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
additional information
?
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3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
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step 3 of 3 of the overall reaction
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3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
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step 3 of 3 of the overall reaction
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
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step 1 of 3 of the overall reaction
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4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
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step 1 of 3 of the overall reaction
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4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
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step 2 of 3 of the overall reaction
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4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
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step 2 of 3 of the overall reaction
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?
additional information
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the enzyme is involved in cholesterol biosynthesis, (24S)-hydroxycholesterol downregulates cholesterol synthesis in neurons, possibly, in a posttranscriptional manner through SREBP-2, overview
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?
additional information
?
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squalene is sterol precursor, 24-methyl and 24-ethyl cholesterol are major sterol compounds in arbuscular mycorrhizal fungi, biosynthetic intermediates are lanosterol and 24-methylene lanosterol
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?
additional information
?
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squalene is sterol precursor, 24-methyl and 24-ethyl cholesterol are major sterol compounds in arbuscular mycorrhizal fungi, biosynthetic intermediates are lanosterol and 24-methylene lanosterol
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additional information
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enzyme is a key enzyme in ergosterol biosynthetic pathway in fungi, determination and analysis of sterol content in wild-type and mutant strains
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additional information
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enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview
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additional information
?
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enzyme plays a key role in sterol biosynthesis as component of the sterol-4-demethylation multienzyme complex, sterol metabolites analysis, overview
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?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cytochrome b5
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dependent on
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NADH
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NADH
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10 to 15% more active with NADH than with NADPH
NADPH
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4alpha-(cyanomethyl)-5alpha-cholestan-3beta-ol
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competitive inhibitor
6-amino-2-n-pentylbenzothiazole
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fenhexamid
fungicide, sterol biosynthesis inhibitor, 9.3 mg/l growth medium (long term exposure for 8 weeks) inhibits germ tube length by 50%, no change in enzyme transcription (0.2-2 mg/l growth medium)
fenpropimorph
fungicide, sterol biosynthesis inhibitor, 6.4 mg/l growth medium (long term exposure for 8 weeks) inhibits germ tube length by 50%, strong decrease in transcription levels with 2 mg/l growth medium, 10 mg/l decreases the fraction of vital stained fungal cells (after 2 and 7 days of treatment), leads to a increase in enzyme mRNA, does not change relative sterol composition and sterol amounts but reduced squalen content by 87% after 7 days of treatment
Trypsin
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30 min treatment, 18% of original concentration of cytochrome b5 left
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additional information
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(24S)-hydroxycholesterol downregulates the expression of several members of the cholesterol/isoprenoid synthesis pathways including the methylsterol monooxygenase, overview
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4alpha-methylsterol monooxygenase deficiency
A rare case of sterol-C4-methyl oxidase deficiency in a young Italian male: Biochemical and molecular characterization.
4alpha-methylsterol monooxygenase deficiency
Disorders of sterol synthesis: beyond Smith-Lemli-Opitz syndrome.
4alpha-methylsterol monooxygenase deficiency
Malformation syndromes caused by disorders of cholesterol synthesis.
4alpha-methylsterol monooxygenase deficiency
New Homozygous Missense MSMO1 Mutation in Two Siblings with SC4MOL Deficiency Presenting with Psoriasiform Dermatitis.
4alpha-methylsterol monooxygenase deficiency
The role of sterol-C4-methyl oxidase in epidermal biology.
Antley-Bixler Syndrome Phenotype
Malformation syndromes caused by disorders of cholesterol synthesis.
Antley-Bixler Syndrome Phenotype
Sterol metabolism disorders and neurodevelopment-an update.
Carcinoma
Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR.
Carcinoma, Ovarian Epithelial
[Analysis of gene expression profiles among 3 epithelial ovarian tumor subtypes using cDNA and tissue microarrays]
Cataract
A rare case of sterol-C4-methyl oxidase deficiency in a young Italian male: Biochemical and molecular characterization.
Cataract
Compound mouse mutants of bZIP transcription factors Mafg and Mafk reveal a regulatory network of non-crystallin genes associated with cataract.
Chondrodysplasia Punctata
Malformation syndromes caused by disorders of cholesterol synthesis.
Chondrodysplasia Punctata
Sterol metabolism disorders and neurodevelopment-an update.
Dermatitis
Mutations in the human SC4MOL gene encoding a methyl sterol oxidase cause psoriasiform dermatitis, microcephaly, and developmental delay.
Dermatitis
New Homozygous Missense MSMO1 Mutation in Two Siblings with SC4MOL Deficiency Presenting with Psoriasiform Dermatitis.
Dermatomycoses
Assessment of the mechanism of drug resistance in Trichophyton mentagrophytes in response to various substances.
Dyslipidemias
Genome-wide association study identifies novel loci association with fasting insulin and insulin resistance in African Americans.
Dyslipidemias
Role of dipeptidyl peptidase IV (DPP4) in the development of dyslipidemia: DPP4 contributes to the steroid metabolism pathway.
Intellectual Disability
New Homozygous Missense MSMO1 Mutation in Two Siblings with SC4MOL Deficiency Presenting with Psoriasiform Dermatitis.
Learning Disabilities
A rare case of sterol-C4-methyl oxidase deficiency in a young Italian male: Biochemical and molecular characterization.
Mevalonate Kinase Deficiency
Sterol metabolism disorders and neurodevelopment-an update.
Microcephaly
A rare case of sterol-C4-methyl oxidase deficiency in a young Italian male: Biochemical and molecular characterization.
Microcephaly
Mutations in the human SC4MOL gene encoding a methyl sterol oxidase cause psoriasiform dermatitis, microcephaly, and developmental delay.
Myopia
New Homozygous Missense MSMO1 Mutation in Two Siblings with SC4MOL Deficiency Presenting with Psoriasiform Dermatitis.
Neoplasms
Axon growth and guidance genes identify T-dependent germinal centre B cells.
Neoplasms
Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGF receptor inhibitors via increased EGF receptor degradation.
Nevus
Sterol metabolism disorders and neurodevelopment-an update.
Obesity
Genome-wide association study identifies novel loci association with fasting insulin and insulin resistance in African Americans.
Psoriasis
The role of sterol-C4-methyl oxidase in epidermal biology.
Smith-Lemli-Opitz Syndrome
Malformation syndromes caused by disorders of cholesterol synthesis.
Smith-Lemli-Opitz Syndrome
Sterol metabolism disorders and neurodevelopment-an update.
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0.125
4alpha-methyl-5alpha-cholestan-3beta-ol
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at pH 7.4 and 37°C
additional information
additional information
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kinetics
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0.18
4alpha-(cyanomethyl)-5alpha-cholestan-3beta-ol
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at pH 7.4 and 37°C
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0.000201
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recovered after Lubrol treatment, 1% of original cytochrome b5 concentration in microsomes, 4-fold that observed for microsomes, maybe cytochrome b5 does not participate in hydroxylation
0.5
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crude extract, at pH 7.4 and °C
17.2
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after 34fold purification, at pH 7.4 and °C
additional information
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enzyme does not involve P-450
additional information
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development of an enzyme assay for specific measurement of enzyme activity using [14C]-4,4-dimethyl-zymosterol as substrate
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6.8
Pcerg25B, sequence calculation
7.36
Pcerg25A, sequence calculation
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Sprague-Dawley
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brenda
endomycorrhizal fungus, grown on chicory (Cichorium intybus) roots
UniProt
brenda
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enzyme is a component of the sterol-4-demethylation multienzyme complex
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UniProt
brenda
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brenda
gene Pcerg25A; a penicillin-producing fungus, gene Pcerg25A
UniProt
brenda
gene Pcerg25B; a penicillin-producing fungus, gene Pcerg25B
UniProt
brenda
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brenda
male, adult
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Sprague-Dawley
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brenda
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enzyme is a component of the sterol-4-demethylation multienzyme complex
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brenda
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brenda
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epidermal
brenda
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brenda
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inhibition of sterol C4methyl oxidase induces activation of the cell cycle
brenda
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brenda
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brenda
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the enzyme forms a demethylation complex in the ER together with the transmembrane protein Erg28p, and possibly the other C-4 demethylation proteins Erg26p, overview
brenda
additional information
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subcellular localization study
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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metabolism
first of three steps to remove 2 C4 methyl groups from an intermediate in ergosterol biosynthesis in the sterol biosynthetic pathway, an enzymatic complex is formed with other enzymes in the pathway
physiological function
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the enzyme regulates epidermal growth factor receptor signaling and vesicular trafficking
malfunction
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deficiency of sterol C4 methyl oxidase, is associated with microcephaly, congenital cataracts, and growth delay in infancy
malfunction
mutations in methyl sterol oxidase cause psoriasiform dermatitis, arthralgias, congenital cataracts, microcephaly, and developmental delay. Enzyme deficiency also substantially alters immunocyte phenotype and in vitro function
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35000
calculated from amino acids
75000
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gel filtration
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?
x * 34 500, Pcerg25B, sequence calculation
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x * 34 873, Pcerg25A, sequence calculation
homodimer
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2 * 36000, SDS-PAGE
homodimer
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2 * 36000, SDS-PAGE
homodimer
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2 * 36000, SDS-PAGE
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additional information
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enzyme is a component of the sterol-4-demethylation multienzyme complex
additional information
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enzyme possesses 3 His-rich motifs common for non-heme diiron-containing enzymes
additional information
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enzyme is a component of the sterol-4-demethylation multienzyme complex
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glycoprotein
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N48D/V133A/F135S
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construction of diverse mutants by random mutagenesis, isolation of the temperature-sensitive mutant strain mERG25 which grows normally at 28°C, but fails to grow at 38°C showing an altered sterol profile
H173Q
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the mutation is associated with sterol C4 methyl oxidase deficiency
H173Q
the mutation is associated with sterol-C4-methyl oxidase deficiency
Y244C
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the mutation is associated with sterol C4 methyl oxidase deficiency
Y244C
the mutation is associated with sterol-C4-methyl oxidase deficiency
additional information
in a complementation assay, the introduced GintSMO gene in methyl oxidase deficient Saccharomyces cerevisiae mutant erg25-25c restores growth in the absence of ergosterol (endproduct of the sterol biosynthesic pathway in the yeast)
additional information
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in a complementation assay, the introduced GintSMO gene in methyl oxidase deficient Saccharomyces cerevisiae mutant erg25-25c restores growth in the absence of ergosterol (endproduct of the sterol biosynthesic pathway in the yeast)
additional information
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construction of a disruption mutant dERG25
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microsomes frozen, buffers contain 1 mM glutathione, no loss of activity
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DEAE-cellulose column chromatography
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extraradial mycelium collected from solubilized medium in buffer (50 mM Tris-HCl, pH 7.5, 10 mM EDTA), vacuum filtrated, frozen in liquid nitrogen
gel filtration, ammonium sulfate fractionation, affinity chromatography
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polyethylene glycol fractionation, DEAE-Sephacel gel filtration, CM-Sepharose column chromatography, and cytochrome b5-Sepharose column chromatography
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DNA and amino acid sequence determination and analysis of wild-type and mutant strains
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DNA is extracted from extraradial mycelium growing around Cichorium intybus, PCR-amplification, cloned into pGEM-T Vector System for sequencing, for complementation assay in Saccharomyces cerevisiae mutant erg25-25c (methyl oxidase deficient) the GintSMO gene is amplified and cloned into the NotI-linearized yeast expression vector pFL61
gene Pcerg25A, DNA and amino acid sequence determination and analysis, Pcerg25A is located in the DNA region containing the penicillin gene cluster, and thus its copy number is dependent on the patterns of the cluster region, up to eight copies of Pcerg25A are found in the high-productivity strain NCPC 10086, functional complementation of the erg25-deficient Saccharomyces cerevisiae strain ATCC 4024690, subcloning in Escherichia coli strain DH5alpha, phylogenetic analysis, overview
gene Pcerg25B, DNA and amino acid sequence determination and analysis, Pcerg25B is present in just a single copy in Penicillium chrysogenum genomes, functional complementation of the erg25-deficient Saccharomyces cerevisiae strain ATCC 4024690, subcloning in Escherichia coli strain DH5alpha, phylogenetic analysis, overview
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agriculture
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enzyme is an antifungal target
medicine
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enzyme is an antifungal target
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Rattus norvegicus
-
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-
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brenda
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