Information on EC 1.14.17.4 - aminocyclopropanecarboxylate oxidase

A nonheme iron enzyme. Requires CO2 for activity. In the enzyme from plants, the ethene has signalling functions such as stimulation of fruit-ripening.

98668-53-2

-

1-amino-2-ethylcyclopropane-1-carboxylate + L-ascorbate + O2

1-butene + cyanide + dehydroascorbate + CO2 + H2O

10 entries

1-amino-2-ethylcyclopropane-1-carboxylate + L-ascorbate + O2 + HCO3-

1-butene + cyanide + dehydroascorbate + CO2 + H2O

3 entries

1-amino-2-methylcyclopropane-1-carboxylate + L-ascorbate + O2

?

9 entries

1-aminocyclopropane-1-carboxylate + 5,6-O-isopropylidine L-ascorbate + O2

ethylene + cyanide + 5,6-O-isopropylidine L-dehydroascorbate + CO2 + H2O

Solanum lycopersicum

-

639301

Substrates: better cosubstrate than L-ascorbate
Products: -

?

1-aminocyclopropane-1-carboxylate + ascorbate + O2

ethene + cyanide + dehydroascorbate + CO2 + 2 H2O

5 entries

1-aminocyclopropane-1-carboxylate + ascorbate + O2

ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O

8 entries

1-aminocyclopropane-1-carboxylate + ascorbate + O2

ethylene + cyanide + dehydroascorbate + CO2 + H2O

34 entries

1-aminocyclopropane-1-carboxylate + ascorbate + O2 + H+

ethylene + cyanide + dehydroascorbate + CO2 + H2O

Solanum lycopersicum

-

727997

Substrates: -
Products: -

?

1-aminocyclopropane-1-carboxylate + D-ascorbate + O2

ethylene + cyanide + D-dehydroascorbate + CO2 + H2O

Solanum lycopersicum

-

639301

Substrates: better cosubstrate than L-ascorbate
Products: -

?

1-aminocyclopropane-1-carboxylate + L-ascorbate + O2

ethylene + cyanide + dehydroascorbate + CO2 + H2O

23 entries

1-aminocyclopropane-1-carboxylic acid + ascorbate + O2

ethylene + cyanide + dehydroascorbate + CO2 + H2O

5 entries

alpha-aminoisobutyric acid + L-ascorbate + O2

ammonia + acetone + dehydroascorbate + CO2 + H2O

Malus domestica

-

639310

Substrates: -
Products: -

?

D-alanine + L-ascorbate + O2

?

Malus domestica

-

639310

Substrates: -
Products: -

?

D-alpha-aminobutyric acid + L-ascorbate + O2

ammonia + acetone + dehydroascorbate + CO2 + H2O

Malus domestica

-

639310

Substrates: -
Products: -

?

L-alanine + L-ascorbate + O2

?

Malus domestica

-

639310

Substrates: -
Products: -

?

L-alpha-aminobutyric acid + L-ascorbate + O2

ammonia + acetone + dehydroascorbate + CO2 + H2O

Malus domestica

-

639310

Substrates: -
Products: -

?

N-hydroxy-alpha-aminoisobutyric acid + L-ascorbate + O2

ammonia + acetone + dehydroascorbate + CO2 + H2O

Malus domestica

-

639310

Substrates: -
Products: -

?

O2 + 1-aminocyclopropyl-1-carboxylic acid + ascorbate

?

Solanum tuberosum

-

698476

Substrates: assay at pH 7.2, 25°C
Products: -

?

additional information

?

-

5 entries

1-aminocyclopropane-1-carboxylate + ascorbate + O2

ethene + cyanide + dehydroascorbate + CO2 + 2 H2O

5 entries

1-aminocyclopropane-1-carboxylate + ascorbate + O2

ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O

8 entries

1-aminocyclopropane-1-carboxylate + ascorbate + O2

ethylene + cyanide + dehydroascorbate + CO2 + H2O

28 entries

1-aminocyclopropane-1-carboxylate + ascorbate + O2 + H+

ethylene + cyanide + dehydroascorbate + CO2 + H2O

Solanum lycopersicum

-

727997

Substrates: -
Products: -

?

1-aminocyclopropane-1-carboxylic acid + ascorbate + O2

ethylene + cyanide + dehydroascorbate + CO2 + H2O

5 entries

ascorbate

20 entries

isoascorbate

Cucumis melo

-

may substitute for ascorbate, 75% of the efficiency of ascorbate

716448

additional information

2 entries

-

Fe2+

25 entries

(1-amino-1-methyl)ethylphosphonic acid

Solanum lycopersicum

-

competitive inhibitor versus both 1-aminocyclopropane-1-carboxylate and bicarbonate

727997

1,10-phenanthroline

2 entries

1-aminocyclopropane-1-carboxylate

Solanum lycopersicum

-

slight inhibition at high concentration

639311

1-aminocyclopropane-1-phosphonic acid

Solanum lycopersicum

-

competitive inhibitor versus both 1-aminocyclopropane-1-carboxylate and bicarbonate

727997

2,4,6-Trinitrobenzenesulfonate

Cucumis melo

-

5 mM, 0.7% residual activity

716448

2,4-dinitrophenol

Cucumis melo

-

1 mM, 40% residual activity

716448

2-aminoisobutyric acid

2 entries

2-aminooxyisobutyric acid

Dianthus caryophyllus

P31528

inhibits the in vitro activities of both 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase in ethylene biosynthetic pathway, 16.3% inhibition of recombinnat enzyme at 10 mM

730269

2-oxoglutarate

Pyrus communis

-

at 1 mM

639306

4-Chloromercuriphenylsulfonate

Cucumis melo

-

1 mM, 1.7% residual activity

716448

8-hydroxyquinoline

Cucurbita maxima

-

639299

alpha-aminoisobutyric acid

4 entries

ascorbate

Pyrus communis

-

required in vitro, inhibitory in vivo

639306

carbonyl sulfide

Cucumis melo

-

in presence of HCO3-, noncompetitive and irreversible, not at CO2-binding site

639298

catalase

Pyrus communis

-

639306

-

Co2+

4 entries

Cu2+

2 entries

cyclopropyl-1-aminocyclopropane-1-carboxylate

3 entries

D-alanine

Malus domestica

-

competitive to 1-aminocyclopropane-1-carboxylate

639310

D-alpha-aminobutyric acid

Malus domestica

-

competitive to 1-aminocyclopropane-1-carboxylate

639310

diethyl dicarbonate

Solanum lycopersicum

-

complete inactivation at 1.5 mM, pH 6.8, after 20 min

EDTA

Mg2+

-

Mn2+

-

N-ethylmaleimide

-

1 mM, 10% residual activity

716448

n-propyl gallate

2 entries

N2

Cucurbita maxima

-

complete inhibition of enzyme in anaerobic N2-atmosphere, reversible

639299

Ni2+

Diospyros kaki

-

659653

p-chloromercuribenzoate

Cucurbita maxima

-

639299

p-chloromercuriphenylsulfonate

Cucurbita maxima

-

639299

pyridoxal 5'-phosphate

Malus domestica

Q00985

a competitive inhibitor of enzyme ACCO with respect to ascorbate. Pyridoxal 5'-phosphate may form a Schiff base with the 1-amino group of lysine blocking a binding site for ascorbate

744060

Salicylhydroxamic acid

Cucumis melo

-

1 mM, 15% residual activity

716448

silver thiosulphate

2 entries

-

Zn2+

3 entries

additional information

2 entries

-

alpha-aminoisobutryric acid

Malus domestica

Q00985

activates enzyme ACCO, prevents enzyme inactivation

ascorbate

bicarbonate

bovine serum albumin

-

stimulates the recombinant enzyme by 41% at 0.1 mM

639301

-

catalase

Solanum lycopersicum

-

recombinant enzyme, stimulates by 36% at 0.5 mg/ml

639301

-

CO2

23 entries

cyanide

Malus domestica

Q00985

activates enzyme ACCO, prevents enzyme inactivation

744060

dihydrojasmonate

Pyrus communis

-

exogenously applied onto fruit skin, it induces the enzyme and the ethylene production in fruits at day 0 of ripening, in the climacteric stage at day 20 of ripening at 20°C the ethylene production increases without enzyme induction in trested and untreated fruits, overview

dithiothreitol

ethylene

indole acetic acid

-

0.1 mM, increases level of ACO2 transcript in vivo, not ACO1

639307

LiCl

Vigna radiata

-

1 mM, increases level of ACO2 transcript in vivo, not ACO1

639307

methyl jasmonate

Solanum lycopersicum

P05116, P24157, A4ZYQ6, Q9ZWP2

0.02 mM methyl jasmonate increases the activity of the enzyme to 148%. 0.2 mM and 1.0 mM methyl jasmonate increases enzyme activity by 46% and 47%, while 0.05 mM induces a smaller elevation of activity by 21% in tomato fruits

728203

n-propyl dihydrojasmonate

-

O2

Triton X-100

additional information

8 entries

-

114

1-amino-2-ethylcyclopropane-1-carboxylate

Diospyros kaki

-

659653

0.012 - 2.3

1-aminocyclopropane-1-carboxylate

54 entries

0.08939 - 0.401

1-aminocyclopropane-1-carboxylic acid

3 entries

1

5,6-O-isopropylidine L-ascorbate

Solanum lycopersicum

-

recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C

639301

14.7

alpha-aminoisobutyrate

Malus domestica

-

recombinant enzyme, pH 7.0, 30°C

639310

0.27 - 2.7

ascorbate

11 entries

1.7

D-ascorbate

Solanum lycopersicum

-

recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C

639301

0.00029 - 0.0053

Fe2+

5 entries

40

HCO3-

Diospyros kaki

-

659653

4.6 - 16.7

L-ascorbate

2 entries

0.028

O2

Solanum lycopersicum

-

recombinant isozyme ACO1, in presence of 0.5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C

639311

additional information

6 entries

-

0.00867 - 5.9

1-aminocyclopropane-1-carboxylate

8 entries

0.0344 - 0.0914

1-aminocyclopropane-1-carboxylic acid

3 entries

0.07

alpha-aminoisobutyrate

Malus domestica

-

recombinant enzyme, pH 7.0, 30°C

639310

0.2

ascorbate

Solanum lycopersicum

-

in 50 mM MOPS, pH 7.0, at 29°C

727997

1.021

O2

plant

-

698476

2.3

(1-amino-1-methyl)ethylphosphonic acid

Solanum lycopersicum

-

in 50 mM MOPS, pH 7.0, at 29°C

727997

38.9

1-aminocyclopropane-1-carboxylate

Solanum lycopersicum

-

recombinant isozyme ACO1, in presence of 5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C

639311

1.5

1-aminocyclopropane-1-phosphonic acid

Solanum lycopersicum

-

in 50 mM MOPS, pH 7.0, at 29°C

727997

4.2 - 7.5

alpha-aminoisobutyric acid

2 entries

28

D-alanine

Malus domestica

-

pH7.0, 30°C

639310

4.2

D-alpha-aminobutyric acid

Malus domestica

-

pH 7.0, 30°C

639310

0.000000064

Malus domestica

Q00985

recombinant His-tagged mutant R175E/T157A, pH 7.2, 20°C

744060

0.0000024

Malus domestica

Q00985

recombinant His-tagged mutant R175E/R244K, pH 7.2, 20°C

744060

0.0000029

Malus domestica

Q00985

recombinant His-tagged mutant R244K/T157A, pH 7.2, 20°C

744060

0.0000033

Malus domestica

Q00985

recombinant His-tagged mutant K158E, pH 7.2, 20°C

744060

0.0000083

Malus domestica

Q00985

recombinant His-tagged mutant R299H, pH 7.2, 20°C

744060

0.00001

Malus domestica

Q00985

recombinant His-tagged mutant S246A/R244K/T157A, pH 7.2, 20°C

744060

0.000012

Malus domestica

Q00985

recombinant His-tagged mutant K158Q/R175Q, pH 7.2, 20°C

744060

0.000021

Malus domestica

Q00985

recombinant His-tagged mutant K158Q/R175E, pH 7.2, 20°C

744060

0.00004

Malus domestica

Q00985

recombinant His-tagged mutant R299L, pH 7.2, 20°C

744060

0.000061

Malus domestica

Q00985

recombinant His-tagged mutant R175E/S246A, pH 7.2, 20°C

744060

0.00007

Malus domestica

Q00985

recombinant His-tagged mutant K158L, pH 7.2, 20°C

744060

0.000083

Malus domestica

Q00985

recombinant His-tagged mutant R175Q, pH 7.2, 20°C

744060

0.00011

Malus domestica

Q00985

recombinant His-tagged mutant K158Q, pH 7.2, 20°C

0.00015

0.00017

recombinant His-tagged mutant R175G, pH 7.2, 20°C

744060

0.0002

Malus domestica

Q00985

recombinant His-tagged mutant T157A, pH 7.2, 20°C

744060

0.00022

Malus domestica

Q00985

recombinant His-tagged mutant K292E, pH 7.2, 20°C

744060

0.00024

Malus domestica

Q00985

recombinant His-tagged mutant R175E, pH 7.2, 20°C

744060

0.00025

Malus domestica

Q00985

recombinant His-tagged mutant K158R, pH 7.2, 20°C

744060

0.00028

Malus domestica

Q00985

recombinant His-tagged mutant R175A, pH 7.2, 20°C

744060

0.00065

Malus domestica

Q00985

recombinant His-tagged mutant R175K, pH 7.2, 20°C

744060

0.00092

Malus domestica

Q00985

recombinant His-tagged mutant R244K/S246A, pH 7.2, 20°C

744060

0.00116

Malus domestica

Q00985

recombinant His-tagged mutant R244K, pH 7.2, 20°C

744060

0.00128

Malus domestica

Q00985

recombinant His-tagged mutant K292R, pH 7.2, 20°C

744060

0.00147

Malus domestica

Q00985

recombinant His-tagged mutant R299K, pH 7.2, 20°C

744060

0.00174

Malus domestica

Q00985

recombinant His-tagged mutant S246A, pH 7.2, 20°C

0.003

0.00348

recombinant His-tagged wild-type enzyme, pH 7.2, 20°C

744060

0.0047

Pyrus communis

-

crude enzyme extract

639312

0.006

Pyrus communis

-

pear fruit prior homogenization

639312

0.008

Malus domestica

-

at 10 mM L-alanine, purified recombinant enzyme

639310

0.011

Malus domestica

-

purified enzyme

639297

0.012

Solanum lycopersicum

P05116, P07920, P24157

partially purified recombinant isozyme ACO3

639308

0.02

Malus domestica

-

purified enzyme

639296

0.023

Malus domestica

-

at 10 mM D-alanine, purified recombinant enzyme

639310

0.025

Solanum lycopersicum

P05116, P07920, P24157

partially purified recombinant isozyme ACO1

639308

0.035

Solanum lycopersicum

-

purified enzyme

639301

0.051

Malus domestica

-

at 10 mM alpha-aminoisobutyric acid, purified recombinant enzyme

639310

0.059

Malus domestica

-

at 10 mM D-alpha-aminobutyric acid, purified recombinant enzyme

639310

0.073

Actinidia deliciosa

P31237

purified recombinant mutant K172C

639303

0.1

Actinidia deliciosa

P31237

purified recombinant mutant K172A

639303

0.11

Actinidia deliciosa

P31237

purified recombinant mutant G137P

639303

0.13

Malus domestica

-

at 10 mM 1-aminocyclopropane-1-carboxylate, purified recombinant enzyme

639310

0.16

Actinidia deliciosa

P31237

purified recombinant wild-type enzyme

639303

additional information

4 entries

6.7

2 entries

6.8 - 7.2

Solanum lycopersicum

P05116, P07920, P24157

-

7

7 - 7.5

7.2

7.4

7.5

7.8

-

additional information

5 entries

5.8 - 8

Solanum lycopersicum

P05116, P07920, P24157

-

6.5 - 8

-

6.5 - 8.5

-

20

assay at

25

28

29

P05116, P07920, P24157

-

30

30 - 35

-

38

-

2 optima at 28°C and 38°C

639312

additional information

Dianthus caryophyllus

-

the enzyme is partially suppressed in petals and completely in gynoecia at 32°C, but expressed at 34°C

4.89

sequence calculation

kiwi

SwissProt

-

UniProt

-

UniProt

L.

-

L.

-

Arabidopsis thaliana Col-0

-

UniProt

-

-

Callistephus chinensis

Nees Hölderin

767246

-

Carica papaya

2 entries

Citrus x aurantiifolia

-

671172

-

Cucumis melo

5 entries

Cucumis melo cv. Andes

-

716629

-

Cucurbita maxima

3 entries

Delphinium grandiflorum

cv. Super Grand Blue

767342

-

Dianthus caryophyllus

Thumb.

-

cv. Grand Rapids

B9A0T8, B9A0T9, B9A0U0

UniProt

fragment; Morus alba L.

UniProt

-

UniProt

-

-

Oryza sativa Japonica Group

-

SwissProt

AVOe3 gene

-

Petunia hybrid cultivar

Petunia hybrida subsp. japonica

SwissProt

-

-

-

SwissProt

plant

-

-

-

-

pv. glycinea, strain ICMP2189, gene efe

684652

A1Z3B9

UniProt

Pseudotsuga menziesii

-

SwissProt

-

-

vetch

-

vascular tissues in the pedicel, the main expression site of LeACO1 is in cell layers just outside the flower pedicel abscission zone in its proximal and distal part

745005

axillary bud

Trifolium repens

-

highest expression in ontological younger tissue

-

Pseudotsuga menziesii

Q1HK31

highest abundance in epithelial cells of cortical resin ducts

-

genes ACO1 and ACO2 are expressed after harvest

-

-

wounded and incubated before enzyme extraction

639299

metaphloem

Zea mays

-

companion cells associated with metaphloem sieve elements

-

Pseudotsuga menziesii

Q1HK31

highest abundance in polyphenolic and ray parenchyma cells

-

expression of LeACO4 is relatively high in the tomato leaf petiole abscission zone

767704

phloem companion cell

Solanum lycopersicum

P05116, P24157

after abscission induction, ACO1 protein is synthesized in phloem companion cells, in which it is localized mainly in the cytoplasm

vascular tissues in the pedicel, the main expression site of LeACO1 is in cell layers just outside the flower pedicel abscission zone in its proximal and distal part

745005

protophloem

Zea mays

-

protophloem sieve elements

700773

receptacle

Delphinium grandiflorum

-

-

relative RNA transcript level 8%

-

relative RNA transcript level 10%

plant

-

698476

stolon tip

Trifolium repens

-

highest expression in ontological younger tissue

plant

-

698476

vascular tissue

2 entries

additional information

-

additional information

5 entries

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

evolution

Agrostis stolonifera

A0A161G0R4

the conserved domains of ACO enzyme from the ACO family of different species are identical by confirming Fe(II)-binding cofactors (His-Xaa-Asp-Xaa-His) and cosubstrate-binding motifs (Arg-Xaa-Ser)

745036

malfunction

Carica papaya

Q5WM32, Q8W2D6

enzyme knockout by RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of papaya fruits, overview

745818

metabolism

7 entries

physiological function

18 entries

additional information

35000

35350

determined by SDA-PAGE and Western blot analysis

700679

35800

Pseudotsuga menziesii

Q1HK31

calculated from cDNA and confirmed by immunoblotting

676720

35820

Solanum lycopersicum

-

electron spray mass spectrometry

639301

36000

Brassica rapa

-

x * 36800, deduced from gene sequence, x * 36000, SDS-PAGE

660076

36310

Malus domestica

Q00985, O48882

determined by SDA-PAGE and Western blot analysis

700679

36800

Brassica rapa

-

x * 36800, deduced from gene sequence, x * 36000, SDS-PAGE

660076

39000

Malus domestica

-

gel filtration

639296

40000

Trifolium repens

-

x * 35000, x * 40000, SDS-PAGE of accumulating recombinant fusion protein

659995

41000

Cucumis melo

-

x * 41000, SDS-PAGE

716448

?

4 entries

monomer

Malus domestica

-

1 * 35000, SDS-PAGE

639296

oligomer

Callistephus chinensis

-

oligomeric enzyme with at least two substrate binding sites

767246

additional information

2 entries

phosphoprotein

Malus domestica

Q00985

ACC oxidase is subject to autophosphorylaton in vitro and promotes phosphorylation of some apple fruit proteins in a ripening-dependent manner

744060

additional information

2 entries

apo-enzyme and in complex with Fe(II) or Co(II)

-

D179E

site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme

639303

G137P

Actinidia deliciosa

P31237

site-directed mutagenesis, 98% activity compared to the recombinant wild-type enzyme

639303

H177Q

Actinidia deliciosa

P31237

site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme

639303

K158A

Actinidia deliciosa

P31237

site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme

639303

K158C

Actinidia deliciosa

P31237

site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme

639303

K172A

Actinidia deliciosa

P31237

site-directed mutagenesis, 85% activity compared to the recombinant wild-type enzyme

639303

K172C

Actinidia deliciosa

P31237

site-directed mutagenesis, 64% activity compared to the recombinant wild-type enzyme

639303

G289S

Agaricus bisporus

H9ZYN5

the enzyme activity of the mutant is 3times as high as that of the wild type enzyme

727091

H216D

Agaricus bisporus

H9ZYN5

the mutant shows 76% of wild type activity

727091

H216D/D218E

Agaricus bisporus

H9ZYN5

the mutant shows 78% of wild type activity

727091

H216D/D218E/H273Q

Agaricus bisporus

H9ZYN5

the mutant does not completely lose catalytic activity

727091

H273Q

Agaricus bisporus

H9ZYN5

the mutant shows 62% of wild type activity

727091

R287G

Agaricus bisporus

H9ZYN5

the activity of the mutant is remarkably decreased to nearly 40% compared to the wild type enzyme

727091

C133A

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

C133P

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme

744060

C165A

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

C283A

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

E294F

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

E297L

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

E301D

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

E301L

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

F187Y

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme

744060

F300Q

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

F300Y

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K144E

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K158E

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K158L

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K158Q

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K158Q/R175E

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K158Q/R175Q

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K158R

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K158R/R175Q

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K172E

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K199E

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K230Q

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K230R

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K230W

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K292E

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme. The low residual activity of the Lys292Glu mutant is typically activated by bicarbonate

744060

K292R

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

K296E

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

N216F

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

P298A

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

Q188A

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

Q188K

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

Q188N

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R175A

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R175E

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R175E/R244K

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R175E/S146A

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R175E/T157A

Malus domestica

Q00985

site-directed mutagenesis, almost inactive mutant

744060

R175G

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R175H

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R175K

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R175Q

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R244A

Malus domestica

-

enzymic activity about 20% of wild-type

657839

R244G

Malus domestica

-

enzymic activity about 20% of wild-type

R244K

R244K/S246A

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R244K/T157A

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R299E

Malus domestica

Q00985

site-directed mutagenesis, inactive mutant

744060

R299H

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R299K

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

R299L

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

S246A

S246A/R244K/T157A

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

S246F

Malus domestica

-

enzymic activity less than 5% of wild-type

657839

S246G

Malus domestica

-

enzymic activity about 50% of wild-type

657839

S246T

Malus domestica

-

enzymic activity less than 5% of wild-type

657839

S246Y

Malus domestica

-

enzymic activity less than 5% of wild-type

657839

S257A

Malus domestica

-

KM-value similar to wild-type

657839

T157A

2 entries

V159G

Malus domestica

-

enzymic activity about 30% of wild-type

657839

W203F

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

Y251F

Malus domestica

Q00985

site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme

744060

C165H

Solanum lycopersicum

P05116

the variant remains active after labeling

766482

C165H/L292C

Solanum lycopersicum

P05116

the double mutant displays activities that ranges between 45 and 60% of the activity of the native (wild-type) enzyme and 60-70% of the activity of the single C165H mutant

766482

C165H/M307C

Solanum lycopersicum

P05116

the double mutant displays activities that ranges between 45 and 60% of the activity of the native (wild-type) enzyme and 60-70% of the activity of the single C165H mutant

766482

D179E

Solanum lycopersicum

-

site-directed mutagenesis, very low activity, stimulation by bicarbonate

639304

D179N

Solanum lycopersicum

-

site-directed mutagenesis, no activity

639304

H177D

Solanum lycopersicum

-

site-directed mutagenesis, no activity

639304

H177D/D179E

Solanum lycopersicum

-

site-directed mutagenesis, no activity

639304

H177E

Solanum lycopersicum

-

site-directed mutagenesis, very low activity, stimulation by bicarbonate

639304

H177Q

Solanum lycopersicum

-

site-directed mutagenesis, no activity

639304

H211Q

Solanum lycopersicum

-

site-directed mutagenesis, slightly reduced activity

639304

H234D

Solanum lycopersicum

-

site-directed mutagenesis, no activity

639304

H234E

Solanum lycopersicum

-

site-directed mutagenesis, no activity

639304

H234Q

Solanum lycopersicum

-

site-directed mutagenesis, no activity

639304

H39Q

Solanum lycopersicum

-

site-directed mutagenesis, slightly reduced activity

639304

H56Q

Solanum lycopersicum

-

site-directed mutagenesis, activity similar to the wild-type

639304

H94Q

Solanum lycopersicum

-

site-directed mutagenesis, slightly reduced activity

639304

additional information

7 entries

enzyme has a short half-life under catalytic conditions undergoing metal-catalyzed oxidative fragmentation

Solanum lycopersicum

-

639304

highly unstable under assay conditions

Pyrus communis

-

639306

unstable, activity decreases during in vitro incubation with half-life of 9 min

Diospyros kaki

-

659653

-20°C, 30% glycerol, stable for 11 days

Pyrus communis

-

639312

2°C, 30% glycerol, loss of 30% activity after 24 h

Pyrus communis

-

639312

2°C, 30% glycerol, loss of 85% activity after 7 h

-

-

180fold to near homogeneity

Malus domestica

-

639296

DEAE Sepharose resin column chromatography

Solanum lycopersicum

-

727997

glutathione-Sepharose chromatography, anion-exchange chromatography

partial

partial, recombinant from Saccharomyces cerevisiae

Solanum lycopersicum

P05116, P07920, P24157

639308

recombinant ACO is purified by affinity chromatography using nickel nitrilotriacetic acid resin, furthermore Sephadex G-25 resin and a Mono-Q column are used, in addition ACO is extracted from apple leaf and fruit tissue

Malus domestica

Q00985, O48882

700679

recombinant enzyme from Escherichia coli to homogeneity

Solanum lycopersicum

-

689795

recombinant from E. coli, 9.8fold to near homogeneity

Solanum lycopersicum

-

639301

recombinant from Escherichia coli

Malus domestica

-

639310

recombinant GST-fusion protein

Trifolium repens

-

659995

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE) plysS by nickel affinity chromatography, ammonium sulfate precipitation

Malus domestica

Q00985

744060

recombinant isozyme ACO1 from Escherichia coli BL21(DE3)-pLysS, to near homogeneity

Solanum lycopersicum

-

639311

recombinant wild-type enzyme and mutants from Escherichia coli

Actinidia deliciosa

P31237

639303

recombinant wild-type enzyme and mutants from Escherichia coli BL21(DE3) to near homogeneity

Solanum lycopersicum

-

639304

-

2 entries

a cDNA library is constructed

Nicotiana suaveolens

Q069K4

700779

expressed in Escherichia coli

Malus domestica

-

639296, 639310

expressed in Escherichia coli BL21(DE3) cells

2 entries

expression in antisense orientation under control of the 35S promoter in plum fruits, var. Bluebyrd and Stanley, by transformation of plum hypocotyls, the transgenic plants show reduced response to exogenous ethylene and delayed fruit ripening and softening in some lines

Prunus persica

-

684223

expression in Escherichia coli

Solanum lycopersicum

-

689795

expression in thermocompetent BL21 (DE3) Escherichia coli cells

Solanum lycopersicum

P05116

766482

expression in transgenic plants

Cucumis melo

-

684222

expression of wild-type and mutants in Escherichia coli

Actinidia deliciosa

P31237

639303

gene ACCO-1, DNA and amino acid sequence determination and analysis

Carica papaya

-

690159

gene ACO, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of FLAG-tagged enzyme ACO, recombinant expression of ACO in transgenic Nicotiana benthamiana plants via tranfection by Agrobacterium tumefaciens strain EHA105 under control of promoter CaMV35S

Agrostis stolonifera

A0A161G0R4

745036

gene ACO-1, expression analysis in gynoecia and petals from three carnation flowers

Dianthus caryophyllus

-

682369

gene ACO1, antisense expression in Petunia x hybrida using the Agrobacterium tumefaciens strain LBA4404 transfection method

Brassica oleracea

Q43404

689726

gene ACO1, differential screening of the petal cDNA library, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons, phylogenetic analysis, expression analysis, overview

Tulipa gesneriana

Q400U9, Q400P0, Q400U7, Q75QN1, Q400V0

689448

gene ACO1, DNA and amino acid sequence determination and analysis, sequence comparison

Carica papaya

Q5WM32, Q8W2D6

745818

gene aco1, quantitative reverse transcription real-time PCR expression analysis

Solanum lycopersicum

P05116, P24157

745005

gene aco1, quantitative RT-PCR expression analysis, analysis of promoter activity of proACO1 in response to GSH and ethephon feeding, expression profiles of ACO1, overview. The activity of ACO1 promoter is unaffected by GSH feeding, but its activity increases significantly on Eth feeding. GSH enhances the mRNA stability of ACO1

Arabidopsis thaliana

Q42560

746110

gene ACO2, differential screening of the petal cDNA library, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons, phylogenetic analysis, expression analysis, overview

Tulipa gesneriana

Q400U9, Q400P0, Q400U7, Q75QN1, Q400V0

689448

gene ACO2, DNA and amino acid sequence determination and analysis, sequence comparison

Carica papaya

Q5WM32, Q8W2D6

745818

gene ACO3, differential screening of the petal cDNA library, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons, phylogenetic analysis, expression analysis, overview

Tulipa gesneriana

Q400U9, Q400P0, Q400U7, Q75QN1, Q400V0

689448

gene ACO4, differential screening of the petal cDNA library, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons, phylogenetic analysis, expression analysis, overview

Tulipa gesneriana

Q400U9, Q400P0, Q400U7, Q75QN1, Q400V0

689448

gene aco4, quantitative reverse transcription real-time PCR expression analysis

Solanum lycopersicum

P05116, P24157

745005

gene ACO5, differential screening of the petal cDNA library, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons, phylogenetic analysis, expression analysis, overview

Tulipa gesneriana

Q400U9, Q400P0, Q400U7, Q75QN1, Q400V0

689448

gene efe, expression under the control of Trichoderma reesei cbh1 promoter in Trichoderma viride strain TL124, protoplast transformation, one copy of efe gene is integrated into the chromosomal DNA, expression analysis

Pseudomonas syringae

A1Z3B9

684652

GST-tagged version expressed in Escherichia coli BL21

Trifolium repens

Q9XER3

716568

into the vector pGEM-T Easy and subsequently into pProEX-1 for expression of the protein in Escherichia coli BL21 cells

Malus domestica

Q00985, O48882

700679

into the vector PMD 18-T for sequencing

Morus alba

Q0PNH5

699794

isozyme ACO1, DNA sequence determination and analysis, expression in Saccharomyces cerevisiae

Solanum lycopersicum

P05116, P07920, P24157

639308

isozyme ACO1, overexpression in Escherichia coli BL21(DE3)-pLysS

Solanum lycopersicum

-

639311

isozyme ACO2, DNA sequence determination and analysis, expression in Saccharomyces cerevisiae

Solanum lycopersicum

P05116, P07920, P24157

639308

isozyme ACO3, DNA sequence determination and analysis, expression in Saccharomyces cerevisiae

Solanum lycopersicum

P05116, P07920, P24157

639308

overexpression in Escherichia coli BL21(DE3)

Solanum lycopersicum

-

639301

recombinant expression in Escherichia coli

Dianthus caryophyllus

P31528

730269

recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE) plysS

Malus domestica

Q00985

744060

wild-type enzyme and mutants, expression in Escherichia coli BL21(DE3)

Solanum lycopersicum

-

639304

ACO1 transcript accumulation in the lower parts of elongating internodes

Oryza sativa

-

716487

ACO3 transcript level in the receptacle increases after ethylene exposure

Delphinium grandiflorum

-

767342

ACO3 transcript level in the receptacle markedly increases during pollination

Delphinium grandiflorum

-

767342

ascorbate feeding fails to increase ACO1 transcript and protein abundance in the pad2-1 mutant

2 entries

damage and low-temperature treatment promote the expression of 1-aminocyclopropane-1-carboxylate oxidase

Morus alba

Q0PNH5

699794

DcACO1 transcript level in the petals is markedly increased by exposure to ethylene. In the style of the control flowers, the DcACO1 transcript level is very low, and ethylene exposure increases the DcACO1 transcript level. In the ovaries of the control flowers, DcACO1 transcript level is low, and the DcACO1 transcript level is rapidly increased

Dianthus caryophyllus

Q39683

767882

in fruits during ripening stage

2 entries

LeACO1 expression is low before abscission induction, and increases 24 h after induction, although with no apparent spatial pattern

Solanum lycopersicum

P05116, P24157

767704

LeACO4 is expressed before abscission induction, and its transcript level declines 24 h after induction on the distal side of the abscission zone fracture

Solanum lycopersicum

P05116, P24157

767704

mRNA level increased in the primary root after transfer to pH 4.0, addition of 1-aminocyclopropane-1-carboxylic acid or indole-3-acetic acid (at pH 6.0) induce accumulation of mRNA

Lactuca sativa

B9A0T8, B9A0T9, B9A0U0

716530

the DcACO2 transcript level is little affected by ethylene exposure. DcACO2 transcript level in petals is increased by ethylene exposure, but its level is much lower than the DcACO1 transcript level. The DcACO2 transcript level in the control and ethylene-treated flowers similarly increases. In the ovaries of the control flowers, DcACO2 transcript level is low

Dianthus caryophyllus

Q39683

767882

the mRNA level and enzyme activity are lower in the 9,10-ketol-octadecadienoic acid-treated fruit than in the untreated control at 119 days after full bloom

Malus sylvestris

-

728645

the transcript levels of the enzyme in gynoecium and receptacle increase during senescence. In the gynoecium, DgACO1 transcript level, which is higher than the other two ACO transcript levels on day 0, increases during senescence. DgACO2 and DgACO3 transcript levels in the gynoecium are almost constant during the first 4 days and increases thereafter. In the receptacle, DgACO1 transcript level increases during the first 4 days and decreases thereafter. DgACO2 transcript level is almost constant during the first 3 days, increases thereafter, and peaks on day 5. DgACO3 transcript level increases starting from day 1 and peaks on day 4

Delphinium grandiflorum

-

767342

transgenic Arabidopsis thaliana phytoalexin deficient2-1 (pad2-1) mutant.plants with depleted GSH content exhibit remarkable downregulation of ACS2, ACS6, and ACO1 at transcript as well as protein levels. The ACO1 gene is remarkably downregulated in pad2-1, a GSH-depleted phytoalexin deficient 2-1 mutant

2 entries

transgenic Arabidopsis thaliana plants with enhanced GSH content exhibit remarkable upregulation of ACS2, ACS6, and ACO1 at transcript as well as protein levels. The ACO1 gene is remarkably upregulated in AtECS1 (plants with enhanced GSH content). Stronger induction of ACS2, ACS6, andACO1 genes is observed in AtECS1 during infection with Bacillus cinerea as well as salt stress, while a weaker induction is observed in pad2-1 mutant

2 entries

white light irradation of dark-grown seedlings following transfer to pH 4.0 induces accumulation of mRNA

Lactuca sativa

B9A0T8, B9A0T9, B9A0U0

716530

agriculture

2 entries

additional information

Dianthus caryophyllus

P31528

potential of 2-aminooxyisobutyric acid as a preservative for carnations and other ornamentals, in which ethylene plays a key role in the induction of senescence, by inhibition of 1-aminocyclopropane-1-carboxylate oxidase. 2-aminooxyisobutyric acid at 1 mM causes 91.5% reduction of maximum ethylene production rate as compared to the control in cut Excerea carnation flowers undergoing senescence, thereby lengthening their vase life to 7 d from 3 d of the control flowers. The compound also extends significantly the vase life of cut flowers of Pax carnation, and tends to do so in Primero Mango carnation. Suppression of flower senescence, overview

730269

ExplorEnz (official portal for IUBMB Enzyme Nomenclature)

Expasy Enzyme Nomenclature Database

KEGG

SABIO-RK

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

Enzyme Nomenclature (alternative site)

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)