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Information on EC 1.14.15.26 - toluene methyl-monooxygenase

for references in articles please use BRENDA:EC1.14.15.26

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IUBMB Comments

The enzyme, characterized from several Pseudomonas strains, catalyses the first step in the degradation of toluenes and xylenes. It has a broad substrate specificity and is also active with substituted compounds, such as chlorotoluenes. The electrons are provided by a reductase (EC 1.18.1.3, ferredoxin-NAD+ reductase) that transfers electrons from NADH via FAD and an [2Fe-2S] cluster. The enzyme can also act on its products, producing gem-diols that spontaneously dehydrate to form aldehydes.

The enzyme appears in viruses and cellular organisms
Reaction Schemes
+
+
2
reduced ferredoxin [iron-sulfur] cluster
+
2
=
+
2
oxidized ferredoxin [iron-sulfur] cluster
+
+
+
2
reduced ferredoxin [iron-sulfur] cluster
+
2
=
+
2
oxidized ferredoxin [iron-sulfur] cluster
+
+
+
2
reduced ferredoxin [iron-sulfur] cluster
+
2
=
+
2
oxidized ferredoxin [iron-sulfur] cluster
+

Synonyms
ntnM, xylM, more

REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
m-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = 3-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
show the reaction diagram
(3)
-
-
-
p-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = 4-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
show the reaction diagram
(2)
-
-
-
toluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = benzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
show the reaction diagram
(1)
-
-
-
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