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Information on EC 1.14.14.86 - ent-kaurene monooxygenase
for references in articles please use BRENDA:EC1.14.14.86
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EC Tree 1 Oxidoreductases
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
1.14.14.86 ent-kaurene monooxygenase
IUBMB Comments
A cytochrome P-450 (heme thiolate) protein found in plants. Catalyses three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.
The enzyme appears in viruses and cellular organisms
- 1.14.14.86
- gibberellin
-
ent-kaurenoic
- fujikuroi
-
ent-copalyl
- rebaudiana
- paclobutrazol
-
3-oxidase
- steviol
- ga20ox
- uniconazole
- biotechnology
-
copalyl
- stevioside
-
8'-hydroxylase
- molecular biology
- synthesis
Reaction Schemes
showSynonyms
ent-kaurene oxidase, kaurene oxidase, osko2, p450-4, cyp701b1, cyp701a, cyp701a26, psko1, cyp701, cyp701a6, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CYP701A
CYP701A3
CYP701A6
CYP701B1
EC 1.14.13.78
-
-
formerly
-
ent-kaurene oxidase
ent-kaurene oxidase-like protein
kaurene oxidase
KO2
OsKO2
additional information
-
the enzyme belongs to the the cytochrome P450 family
ent-kaurene oxidase
-
-
misleading
-
OsKO2
-
formerly named OsKOL2, OsKO1 expression is restricted to panicles and roots
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 = ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
(1c)
-
-
-
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 = ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
(1b)
-
-
-
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 = ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
(1a)
-
-
-
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction
-
-
-
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction, reaction mechanism of multifunctional kaurene oxidase, overview
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
ent-kaur-16-ene,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (hydroxylating)
A cytochrome P-450 (heme thiolate) protein found in plants. Catalyses three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.
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CAS REGISTRY NUMBER
COMMENTARY
149565-67-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
16alpha-hydroxy-ent-kaurane + [reduced NADPH-hemoprotein reductase] + O2
16alpha-hydroxy-ent-kauranoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
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?
ent-beyerene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
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?
ent-cassadiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-cassadien-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
Products: -
Products: -
?
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
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?
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
ent-cassadien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene + 3 NADPH + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: overall reaction
Products: -
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?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
ent-sandaracopimaradiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-sandaracopimaradien-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
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?
ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
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?
ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
ent-sandaracopimaradien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
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?
ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
isokaurene + [reduced NADPH-hemoprotein reductase] + O2
isokauren-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
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?
isopimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
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?
isopimara-8,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
3beta-hydroxyisopimara-8,15-diene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
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?
isopimara-8,15-diene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
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?
sandaracopimaradiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
3beta-hydroxysandaracopimaradiene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
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?
sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
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?
syn-aphidicolene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
3beta-hydroxy-syn-aphidicolene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
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?
syn-aphidicolene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
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?
syn-labdatriene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
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?
syn-pimara-7,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
3beta-hydroxy-syn-pimaradiene + 2alpha-hydroxy-syn-pimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
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?
syn-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
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?
syn-pimara-9(11),15-diene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: low activity
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?
syn-pimaradiene + [reduced NADPH-hemoprotein reductase] + O2
3beta-hydroxy-syn-pimaradiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
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?
syn-stemarene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
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?
syn-stemodene + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
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?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: a three step oxidation
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?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q68YV8
Substrates: the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
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?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q68YV8
Substrates: a three step oxidation
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?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q6GZ42, Q68YV9, Q68YV7
Substrates: the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
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?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q6GZ42, Q68YV9, Q68YV7
Substrates: a three step oxidation
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?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: last step in gibberellic acid biosynthesis, before the branch point for steviol production, pathway overview
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?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: a three step oxidation
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?
ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
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?
ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
Products: -
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?
ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
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?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: three step oxidation process via ent-kaurenol and ent-kaurenal, in co-operation with membrane-associated cytochrome P450 monooxygenase
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?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
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?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
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?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: deuterium-labeled substrate, 5 mM NADPH, 1 mM EDTA, 20% glycerol, 1 mM DTT, 50 mM potassium phosphate buffer, pH 7.0, 21°C, 30% yield of end-product ent-kaurenoate with ent-kaurenol as substrate
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ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
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?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
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?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
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?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: overall reaction
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: overall reaction
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: -
Products: -
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: overall reaction
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: -
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: overall reaction
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: overall reaction, substrate is isolated from leaves of Cryptomeria japonica
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: overall reaction
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
Products: -
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: overall reaction
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: -
Products: -
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ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: overall reaction
Products: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: part of gibberelin biosynthetic pathway, decreased KO levels increase the sensitivity to KO inhibitors
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
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?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: involved in gibberilin biosynthesis pathway
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: deuterium-labeled substrate, 5 mM NADPH, 1 mM EDTA, 20% glycerol, 1 mM DTT, 50 mM potassium phosphate buffer, pH 7.0, 21°C, 6% yield of end-product ent-kaurenoate with ent-kaurene as substrate
Products: -
Products: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: involved in gibberilin biosynthesis pathway
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
Substrates: involved in gibberilin biosynthesis pathway
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
Products: -
Products: -
?
ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
Products: -
Products: -
?
ent-trachylobane + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: -
Products: -
Products: -
?
ent-trachylobane + [reduced NADPH-hemoprotein reductase] + O2
?
Substrates: low activity
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
Products: -
Products: -
?
additional information
?
-
Substrates: use of combinatorial biosynthesis enabled analysis of more than 20 potential substrates, starting with (E,E,E)-geranylgeranyl diphosphate, as well as structural characterization of 12 resulting unknown products, determination of hydroxylation positions, structure-function relationship for the activity, and substrate specificity, overview. No activity with ent-pimara-8(14),15-diene, pimara-8,15-diene, miltiradiene, abietaenol, sclarene, labdadienol, and ent-kauran-16beta-ol
Products: -
Products: -
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additional information
?
-
Substrates: the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
Products: -
Products: -
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additional information
?
-
Substrates: the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
Products: -
Products: -
?
additional information
?
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Substrates: the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
Products: -
Products: -
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additional information
?
-
Q68YV8
Substrates: the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
Products: -
Products: -
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additional information
?
-
-
Substrates: CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
Products: -
Products: -
?
additional information
?
-
Q6GZ42
Substrates: the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
Products: -
Products: -
?
additional information
?
-
Q68YV9
Substrates: the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
Products: -
Products: -
?
additional information
?
-
Q68YV7
Substrates: the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
Products: -
Products: -
?
additional information
?
-
Substrates: use of combinatorial biosynthesis enabled analysis of more than 20 potential substrates, starting with (E,E,E)-geranylgeranyl diphosphate, as well as structural characterization of 12 resulting unknown products, determination of hydroxylation positions, structure-function relationship for the activity, and substrate specificity, overview. No activity with ent-pimara-8(14),15-diene, pimara-8,15-diene, miltiradiene, abietaenol, sclarene, labdadienol, ent-kauran-16beta-ol, ent-cassadiene, ent-sandaracopimaradiene, syn-aphidicolene, isopimara-7,15-diene, ent-beyerene, syn-pimara-7,15-diene, syn-stemarene, syn-stemodene, syn-labdatriene, syn-pimara-9(11),15-diene, isopimara-8,15-diene, sandaracopimaradiene
Products: -
Products: -
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additional information
?
-
-
Substrates: CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
Products: -
Products: -
?
additional information
?
-
-
Substrates: GC-MS or GLC product identification
Products: -
Products: -
?
additional information
?
-
Substrates: GC-MS or GLC product identification
Products: -
Products: -
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additional information
?
-
-
Substrates: enzyme catalyzes the oxidation of ent-kaurene to entkaurenoic acid through three successive monooxygenations. Te rate-limiting step of this oxidation might be the initial step that forms ent-kaurenol
Products: -
Products: -
?
additional information
?
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Substrates: enzyme catalyzes the oxidation of ent-kaurene to entkaurenoic acid through three successive monooxygenations. Te rate-limiting step of this oxidation might be the initial step that forms ent-kaurenol
Products: -
Products: -
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additional information
?
-
-
Substrates: the enzyme is involved in sweet steviol glycoside synthesis, the diterpene steviol which is produced from a branch of the gibberellic acid biosynthetic pathway, spatial organization, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid. It can also convert 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid
Products: -
Products: -
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additional information
?
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-
Substrates: CYP701A26 products are ent-kaurene derivatives with sequential oxidation at C19, i.e. ent-kauren-19-ol, ent-kauren-19-al, ent-kauren-19-oic acid
Products: -
Products: -
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
16alpha-hydroxy-ent-kaurane + [reduced NADPH-hemoprotein reductase] + O2
16alpha-hydroxy-ent-kauranoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene + 3 NADPH + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: overall reaction
Products: -
Products: -
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: involved in gibberilin biosynthesis pathway
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q68YV8
Substrates: the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
Products: -
Products: -
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(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Q6GZ42, Q68YV9, Q68YV7
Substrates: the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins
Products: -
Products: -
?
(-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
(-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: last step in gibberellic acid biosynthesis, before the branch point for steviol production, pathway overview
Products: -
Products: -
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ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: three step oxidation process via ent-kaurenol and ent-kaurenal, in co-operation with membrane-associated cytochrome P450 monooxygenase
Products: -
Products: -
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ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: overall reaction
Products: -
Products: -
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: overall reaction
Products: -
Products: -
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: overall reaction
Products: -
Products: -
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: overall reaction
Products: -
Products: -
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: overall reaction
Products: -
Products: -
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
Substrates: overall reaction
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: part of gibberelin biosynthetic pathway, decreased KO levels increase the sensitivity to KO inhibitors
Products: -
Products: -
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ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: involved in gibberilin biosynthesis pathway
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-al + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
Substrates: involved in gibberilin biosynthesis pathway
Products: -
Products: -
?
ent-kaur-16-ene-19-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: -
Products: -
Products: -
?
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
Products: -
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?
additional information
?
-
-
Substrates: the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
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?
additional information
?
-
Substrates: the enzyme catalyzes three hydroxylation reactions, which further requires dehydration at some stage, overview
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Products: -
?
additional information
?
-
Substrates: the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme is involved in the biosynthetic pathway and metabolism of gibberellins. Gibberellin mediates the control of seed germination by phytochrome mainly via the control of the endogenous content of bioactive gibberellic acid, rather than the control of gibberellic acid responsiveness, gibberellin metabolism and signalling in lettuce seed, overview
Products: -
Products: -
?
additional information
?
-
Q68YV8
Substrates: the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
Products: -
Products: -
?
additional information
?
-
Q6GZ42
Substrates: the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
Products: -
Products: -
?
additional information
?
-
Q68YV9
Substrates: the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
Products: -
Products: -
?
additional information
?
-
Q68YV7
Substrates: the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in sweet steviol glycoside synthesis, the diterpene steviol which is produced from a branch of the gibberellic acid biosynthetic pathway, spatial organization, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid. It can also convert 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid
Products: -
Products: -
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cytochrome P450
typical ferric low-spin cytochrome P450, which is completely moved to high-spin state upon binding with ent-kaurene
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
purified ferric CYP701B1 is a typical low-spin cytochrome P450 showing a characteristic absorption peak
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(1E)-1-[4-(4-butyl-1H-1,2,3-triazol-1-yl)phenyl]-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol
-
(1E,3S)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol
i.e. uniconazole
(5E)-6-tert-butyl-5-[(4-chlorophenyl)methylidene]-5,6-dihydro-8H-[1,2,4]triazolo[5,1-c][1,4]oxazine
-
(5E)-6-tert-butyl-5-[(4-nitrophenyl)methylidene]-5,6-dihydro-8H-[1,2,4]triazolo[5,1-c][1,4]oxazine
-
(E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate
-
i.e. abscinazole-E1, orUT1-E2Ts, or Abz-E1, a specific potent inhibitor of ABA 8'-hydroxylase, EC 1.14.13.93, but a weak inhibitor of CYP701A, both in vitro and in vivo
methyl (2E)-3-[1-[(1E)-1-(4-chlorophenyl)-3-hydroxy-4,4-dimethylpent-1-en-2-yl]-1H-imidazol-5-yl]prop-2-enoate
-
uniconazole
selective binding of CYP701B1 with uniconazole, is directly bound to the heme iron with its azole nitrogen
additional information
-
among the azoles known as CYP51 inhibitors, ketoconazole, an antifungal medicine, is one of the most potent CYP51 inhibitor and forms a one-to-one complex with CYP51 as with uniconazole and CYP701B1. But ketoconazole does not bind and inhibit the purified ent-kaurene oxidase, CYP701B1; not inhibitory: ketoconazole
-
additional information
among the azoles known as CYP51 inhibitors, ketoconazole, an antifungal medicine, is one of the most potent CYP51 inhibitor and forms a one-to-one complex with CYP51 as with uniconazole and CYP701B1. But ketoconazole does not bind and inhibit the purified ent-kaurene oxidase, CYP701B1; not inhibitory: ketoconazole
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cytochrome P450 monooxygenase
-
necessary for ent-kaurenoate production by ent-kaurene oxidase
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.03
isopimara-7,15-diene
pH and temperature not specified in the publication
0.03
isopimara-8,15-diene
pH not specified in the publication, temperature not specified in the publication
0.05
ent-cassadiene
pH and temperature not specified in the publication
0.05
ent-cassadiene
pH not specified in the publication, temperature not specified in the publication
0.0091
ent-kaur-16-ene
pH 7.5, 30°C, recombinant His-tagged enzyme
0.046
ent-sandaracopimaradiene
pH and temperature not specified in the publication
0.046
ent-sandaracopimaradiene
pH not specified in the publication, temperature not specified in the publication
0.05
syn-aphidicolene
pH and temperature not specified in the publication
0.05
syn-aphidicolene
pH not specified in the publication, temperature not specified in the publication
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00097
(E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate
-
pH 7.25, 30°C, recombinant enzyme
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.000231
(5E)-6-tert-butyl-5-[(4-chlorophenyl)methylidene]-5,6-dihydro-8H-[1,2,4]triazolo[5,1-c][1,4]oxazine
Oryza sativa Japonica Group
pH not specified in the publication, temperature not specified in the publication
0.000153
(5E)-6-tert-butyl-5-[(4-nitrophenyl)methylidene]-5,6-dihydro-8H-[1,2,4]triazolo[5,1-c][1,4]oxazine
Oryza sativa Japonica Group
pH not specified in the publication, temperature not specified in the publication
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
2.19 Bq kaurenoids/min/mg, activity in cell extracts
additional information
Q68YV8
gibberellic acid content in healthy and rice dwarf virus-infected plants
additional information
Q6GZ42
gibberellic acid content in healthy and rice dwarf virus-infected plants
additional information
Q68YV9
gibberellic acid content in healthy and rice dwarf virus-infected plants
additional information
Q68YV7
gibberellic acid content in healthy and rice dwarf virus-infected plants
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
i.e. Gibberella fujikuroi or G. intermedia, MP-C or MP-D, strains IMI58289 and D02945, gene P450-4
SwissProt
brenda
ent-kaurene oxidase 1; cv. Grand Rapids, genes LsKO1 and LsKO2 encoding ent-kaurene oxidases 1 and 2
UniProt
brenda
ent-kaurene oxidase 2; cv. Grand Rapids, genes LsKO1 and LsKO2 encoding ent-kaurene oxidases 1 and 2
UniProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
the enzyme expression in pistillate flowers is significantly higher than in staminate flowers and neutral flowers
brenda
relative transcript levels of KO2 observed in roots is approximately 50-fold to 60-fold higher than those in young leaves
brenda
mainly expressed in the root and lateral root systems
brenda
relative transcript levels of KO2 observed in roots is approximately 50-fold to 60-fold higher than those in young leaves
brenda
additional information
the expression level is especially abundant in root and stem apexes
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
enzyme is targeted to the outer chloroplast envelope
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
physiological function
additional information
metabolism
kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid
metabolism
-
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid as an early step in gibberellin (GA) biosynthesis, gibberellin phytohormone biosynthesis, pathway overview
metabolism
oxidation of ent-kaurene to ent-kaurenoic acid is a common process in gibberellin biosynthesis by vascular plants and production of gibberellin-like compounds by nonvascular plants
metabolism
the enzyme is involved in the in the gibberellin biosynthetic pathway, it catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid
metabolism
-
oxidation of ent-kaurene to ent-kaurenoic acid is involved in gibberellin biosynthesis
metabolism
the enzyme is involved in gibberellin biosynthesis
metabolism
-
kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid
-
physiological function
kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid
physiological function
-
the enzyme catalyzes the three-step oxidation of the C4alpha methyl of the tetracyclic diterpene intermediate ent-kaurene to form ent-kaurenoic acid as an early step in gibberellin (GA) biosynthesis. It can also convert 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid, indicating an important function of 16alpha-hydroxy-ent-kaurane in the anti-HIV principle tripterifordin biosynthetic pathway in planta
physiological function
-
the enzyme is involved in gibberellin biosynthesis and catalyzes carboxylation on C19 of ent-kaurene to generate ent-kaurenoic acid in plastids. The tetracyclic diterpenoid plant hormones, gibberellins, play important roles during the entire life cycle of higher plants including cell elongation, flowering and fruit set, seed germination, and many other developmental processes
physiological function
the multiply reactive cytochrome P450, ent-kaurene oxidase (KO), is involved in production of the diterpenoid plant hormone gibberellin
physiological function
natural mutant d35Tan-Ginbozu mutant plants reached 6070% of the height of wild-type throughout the vegetative development. All internodes of d35Tan-Ginbozu are shortened in comparison to the wild-type, mutation induces stunted culms by gibberellic acid deficiency
physiological function
the multiply reactive cytochrome P450, ent-kaurene oxidase (KO), is involved in production of the diterpenoid plant hormone gibberellin
physiological function
in the gibberellin biosynthetic pathway. CPSent catalyzes the cyclization of geranylgeranyl diphosphate to ent-copalyl diphosphate, which is converted to ent-kaurene by kaurene synthase. Then, kaurene oxidase catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid
physiological function
-
kaurene oxidase is a multifunctional cytochrome P450 catalysing three sequential oxidations in gibberellin phytohormone biosynthesis. These serve to transform the C4alpha methyl of the ent-kaurene olefin intermediate into the carboxylic acid moiety of ent-kauren-19-oic acid
-
additional information
-
enzyme structur homology modeling and molecular docking, using the crystal structure of cytochrome P450 monooxygenase CYP170A1 from Streptomyces coelicolor, PDB ID 3DBG, as template. Key residue Leu387 contributes to the formation of 16alpha-hydroxy-ent-kaurenoic acid, most likely by forming hydrogen bonds with the hydroxyl group of 16alpha-hydroxy-ent-kaurane, multifunctional nature of kaurene oxidase catalysis, overview
additional information
a modular metabolic engineering system is used in a combinatorial biosynthetic approach to detect substrates of ent-kaurene oxidase, overview
additional information
a modular metabolic engineering system is used in a combinatorial biosynthetic approach to detect substrates of ent-kaurene oxidase, overview
Show AA Sequence and Transmembrane Helices (348 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
L387A
-
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
L387D
-
site-directed mutagenesis, the mutation causes the loss of the specific catalytic function from 16alpha-hydroxy-ent-kaurane to 16a-hydroxy-ent-kaurenoic acid
L387G
-
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
L387R
-
site-directed mutagenesis, the mutation causes the loss of the specific catalytic function from 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid
L387S
-
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
L387T
-
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type
additional information
additional information
usage of a fully codon-optimized construct, along with additional N-terminal deletion and modification, for functional recombinant expression in Escherichia coli, recombinant engineered enzyme is used to carry out 18O2 labelling studies with ent-kaurene, and the intermediates ent-kaurenol and ent-kaurenal, to investigate the multifunctional reaction sequence, overview
additional information
-
usage of a fully codon-optimized construct, along with additional N-terminal deletion and modification, for functional recombinant expression in Escherichia coli, recombinant engineered enzyme is used to carry out 18O2 labelling studies with ent-kaurene, and the intermediates ent-kaurenol and ent-kaurenal, to investigate the multifunctional reaction sequence, overview
additional information
-
usage of a fully codon-optimized construct, along with additional N-terminal deletion and modification, for functional recombinant expression in Escherichia coli, recombinant engineered enzyme is used to carry out 18O2 labelling studies with ent-kaurene, and the intermediates ent-kaurenol and ent-kaurenal, to investigate the multifunctional reaction sequence, overview
-
additional information
mutation d35Tan-Ginbozu is a single nucleotide substitution located in exon 5, leading to a replacement of arginine by serine
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant CYP701A3 partially in microsomal membranes from Escherichia coli
recombinant His-tagged enzyme from Pichia pastoris microsomes by ultracentrifugation, nickel affinity chromatography, and dialysis
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned from leaf microsomes, functional recombinant expression in Saccharomyces cerevisiae
-
cloning of the enzyme from hairy roots of Salvia miltiorrhiza, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, SmKO is co-expressed with the fused enzyme SmKS-SmCPSent and a NADPH-cytochrome P450 reductase (SmCPR1) in the yeast strain SGH5
CYP701A, expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method
-
CYP701A3, functional expression in Escherichia coli using a fully codon-optimized construct, along with additional N-terminal deletion and modification
DNA and amino acid sequence determimnation and analysis, expression in Escherichia coli, Saccharomyces cerevisiae, and in transgenic Arabidopsis thaliana leaves as GFP-tagged protein via Agrobacterium tumefaciens transfection, expression of the GFP-tagged enzyme in onion bulb epidermis or tobacco seedling leaves using particle bombardment method
-
DNA and amino acid sequence determination and analysis, sequence comparisons
Q6GZ42, Q68YV9, Q68YV7
expression in Escherichia coli
gene CYP701A26, cloning from seedlings, DNA and amino acid sequence determination and analysis, phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis
-
gene CYP701B1, genetic structure, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris microsomes
gene LsKO1, DNA and amino acid sequence determination and analysis, quantitative PCR expression analysis, expression in Pichia pastoris
gene LsKO2, DNA and amino acid sequence determination and analysis, quantitative PCR expression analysis, expression in Pichia pastoris
gene P450-4 from strain D02945, DNA and amino acid sequence determination and analysis, organization of the GA gene cluster, expression in Escherichia coli and in strain D02945, screening for ectopic integrations, P450-4 promoter studies using the GUS reporter system, expression analysis
KOS2, DNA and amino acid sequence determination and analysis, sequence comparisons
Q6GZ42, Q68YV9, Q68YV7
KOS3, DNA and amino acid sequence determination and analysis, sequence comparisons
Q68YV8
KOS4, DNA and amino acid sequence determination and analysis, sequence comparisons
Q6GZ42, Q68YV9, Q68YV7
PCR-amplification of cDNA, cloning into pPICZ yeast expression vector, expression in Pichia pastoris strain X-33 together with fungal P450 reductase (CPR)
-
phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant ent-kaurene oxidase expression in Saccharomyces cerevisiae strain BY-T20, coexpression with Tripterygium wilfordii CPR1-4 genes encoding four cytochrome P450 reductases, recombinant expression of enzyme mutants
-
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
molecular biology
-
the fungal cytochrome P450 monooxygenase isolated from from Phaeoshaeria sp., strain L487 in the Pichia pastoris expression system might become a tool for functional assays of a variety of rice cytochrome P450 monooxygenases involved in the biosynthesis of secondary metabolites
synthesis
biotechnology
Q6GZ42, Q68YV9, Q68YV7
P2 may provide a tool to investigate the regulation of GA metabolism for plant growth and development via affection of ent-kaurene oxidase
biotechnology
Q68YV8
P2 may provide a tool to investigate the regulation of GA metabolism for plant growth and development via affection of ent-kaurene oxidase
synthesis
when KO2 is coexpressed in Escherichia coli engineered to produce ent-kaurene, turnover to entkauren-19-oic acid is observed. In addition, KO2 reacts with ent-isokaurene, and the closely related ent-trachylobane, converting the C4alpha methyl to a mixture of the alcohol and further oxidized carboxylic acid with each substrate
synthesis
when KO2 is coexpressed in Escherichia coli engineered to produce ent-kaurene, turnover to entkauren-19-oic acid is observed. In addition, KO2 reacts with ent-isokaurene, and the closely related ent-trachylobane, converting the C4alpha methyl to a mixture of the alcohol and further oxidized carboxylic acid with each substrate
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sherwin, P.F.; Coates, R.M.
Stereospecificity of the oxidation of ent-kauren-19-ol to ent-kaurenal by a microsomal enzyme preparation from Marah macrocarpus
J. Chem. Soc.
1982
1013-1014
1982
Marah macrocarpa
-
brenda
Ashman, P.J.; Mackenzie, A.; Bramley, P.M.
Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi
Biochim. Biophys. Acta
1036
151-157
1990
Cucurbita maxima, Fusarium fujikuroi, Fusarium fujikuroi SG78, Malus sp.
brenda
Archer, C.; Ashman, P.J.; Hedden, P.; Bowyer, J.R.; Bramley, P.M.
Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima
Biochem. Soc. Trans.
20
218S
1992
Cucurbita maxima, Fusarium fujikuroi
brenda
Helliwell, C.A.; Poole, A.; Peacock, W.J.; Dennis, E.S.
Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis
Plant Physiol.
119
507-510
1999
Arabidopsis thaliana
brenda
Helliwell, C.A.; Sullivan, J.A.; Mould, R.M.; Gray, J.C.; Peacock, W.J.; Dennis, E.S.
A plastid envelope location of Arabidopsis ent-kaurene oxidase links the plastid and endoplasmic reticulum steps of the gibberellin biosynthesis pathway
Plant J.
28
201-208
2001
Arabidopsis thaliana (Q93ZB2), Arabidopsis thaliana
brenda
Tudzynski, B.; Hedden, P.; Carrera, E.; Gaskin, P.
The P450-4 gene of Gibberella fujikuroi encodes ent-kaurene oxidase in the gibberellin biosynthesis pathway
Appl. Environ. Microbiol.
67
3514-3522
2001
Fusarium fujikuroi, Fusarium fujikuroi (O94142)
brenda
Swain, S.M.; Singh, D.P.; Helliwell, C.A.; Poole, A.T.
Plants with increased expression of ent-kaurene oxidase are resistant to chemical inhibitors of this gibberellin biosynthesis enzyme
Plant Cell Physiol.
46
284-291
2005
Arabidopsis thaliana
brenda
Davidson, S.E.; Smith, J.J.; Helliwell, C.A.; Poole, A.T.; Reid, J.B.
The pea gene LH encodes ent-kaurene oxidase
Plant Physiol.
134
1123-1134
2004
Lathyrus oleraceus (Q6XAF4)
brenda
Sakamoto, T.; Miura, K.; Itoh, H.; Tatsumi, T.; Ueguchi-Tanaka, M.; Ishiyama, K.; Kobayashi, M.; Agrawal, G.K.; Takeda, S.; Abe, K.; Miyao, A.; Hirochika, H.; Kitano, H.; Ashikari, M.; Matsuoka, M.
An overview of gibberellin metabolism enzyme genes and their related mutants in rice
Plant Physiol.
134
1642-1653
2004
Oryza sativa
brenda
Malonek, S.; Rojas, M.C.; Hedden, P.; Gaskin, P.; Hopkins, P.; Tudzynski, B.
Functional characterization of two cytochrome P450 monooxygenase genes, P450-1 and P450-4, of the gibberellic acid gene cluster in Fusarium proliferatum (Gibberella fujikuroi MP-D)
Appl. Environ. Microbiol.
71
1462-1472
2005
Fusarium proliferatum (Q701P2), Fusarium proliferatum
brenda
Humphrey, T.V.; Richman, A.S.; Menassa, R.; Brandle, J.E.
Spatial organisation of four enzymes from Stevia rebaudiana that are involved in steviol glycoside synthesis
Plant Mol. Biol.
61
47-62
2006
Stevia rebaudiana
brenda
Zhu, S.; Gao, F.; Cao, X.; Chen, M.; Ye, G.; Wei, C.; Li, Y.
The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms
Plant Physiol.
139
1935-1945
2005
Oryza sativa (Q68YV8), Oryza sativa Japonica Group (Q6GZ42), Oryza sativa Japonica Group (Q68YV9), Oryza sativa Japonica Group (Q68YV7)
brenda
Sawada, Y.; Katsumata, T.; Kitamura, J.; Kawaide, H.; Nakajima, M.; Asami, T.; Nakaminami, K.; Kurahashi, T.; Mitsuhashi, W.; Inoue, Y.; Toyomasu, T.
Germination of photoblastic lettuce seeds is regulated via the control of endogenous physiologically active gibberellin content, rather than of gibberellin responsiveness
J. Exp. Bot.
59
3383-3393
2008
Lactuca sativa (B5MEX5), Lactuca sativa (B5MEX6)
brenda
Ko, K.W.; Lin, F.; Katsumata, T.; Sugai, Y.; Miyazaki, S.; Kawaide, H.; Okada, K.; Nojiri, H.; Yamane, H.
Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system
Biosci. Biotechnol. Biochem.
72
3285-3288
2008
Oryza sativa
brenda
Morrone, D.; Chen, X.; Coates, R.M.; Peters, R.J.
Characterization of the kaurene oxidase CYP701A3, a multifunctional cytochrome P450 from gibberellin biosynthesis
Biochem. J.
431
337-344
2010
Arabidopsis thaliana (Q93ZB2), Arabidopsis thaliana, Arabidopsis thaliana Columbia (Q93ZB2)
brenda
Okazaki, M.; Nimitkeatkai, H.; Muramatsu, T.; Aoyama, H.; Ueno, K.; Mizutani, M.; Hirai, N.; Kondo, S.; Ohnishi, T.; Todoroki, Y.
Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8-hydroxylase CYP707A
Bioorg. Med. Chem.
19
406-413
2011
Oryza sativa, Oryza sativa Nipponbare
brenda
Lei, L.; Fan, Z.; Tang, L.; Chen, F.
Molecular cloning and identification of tissue-specific expression of ent-kaurene oxidase gene in Momordica charantia
Afr. J. Biotechnol.
10
15724-15730
2011
Momordica charantia (D5LXQ4)
-
brenda
Miyazaki, S.; Katsumata, T.; Natsume, M.; Kawaide, H.
The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P
FEBS Lett.
585
1879-1883
2011
Physcomitrium patens (F2ZAE0), Physcomitrium patens
brenda
Hamberger, B.; Ohnishi, T.; Hamberger, B.; Seguin, A.; Bohlmann, J.
Evolution of diterpene metabolism: Sitka spruce CYP720B4 catalyzes multiple oxidations in resin acid biosynthesis of conifer defense against insects
Plant Physiol.
157
1677-1695
2011
Picea sitchensis
brenda
Wang, Q.; Hillwig, M.L.; Wu, Y.; Peters, R.J.
CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism
Plant Physiol.
158
1418-1425
2012
Oryza sativa
brenda
Villa-Ruano, N.; Lozoya-Gloria, E.; Betancourt-Jimenez, M.; Herlt, T.; Pacheco-Hernandez, Y.; Castro-Juarez, C.
Functional characterization of ent-kaurene oxidase, MtKO, from Montanoa tomentosa (zoapatle)
Arch. Biol. Sci.
67
193-199
2015
Montanoa tomentosa
-
brenda
Todoroki, Y.; Naiki, K.; Muramatsu, T.; Ohnishi, T.; Ueno, K.; Mizutani, M.; Hirai, N.
A conformationally restricted uniconazole analogue as a specific inhibitor of rice ent-kaurene oxidase, CYP701A6
Bioorg. Med. Chem. Lett.
22
3240-3243
2012
Oryza sativa Japonica Group (Q5Z5R4)
brenda
Mao, H.; Shen, Q.; Wang, Q.
CYP701A26 is characterized as an ent-kaurene oxidase with putative involvement in maize gibberellin biosynthesis
Biotechnol. Lett.
39
1709-1716
2017
Zea mays
brenda
Su, P.; Guan, H.; Zhang, Y.; Wang, X.; Gao, L.; Zhao, Y.; Hu, T.; Zhou, J.; Ma, B.; Tu, L.; Tong, Y.; Huang, L.; Gao, W.
Probing the single key amino acid responsible for the novel catalytic function of ent-kaurene oxidase supported by NADPH-cytochrome P450 reductases in Tripterygium wilfordii
Front. Plant Sci.
8
1756
2017
Tripterygium wilfordii
brenda
Noguchi, C.; Miyazaki, S.; Kawaide, H.; Gotoh, O.; Yoshida, Y.; Aoyama, Y.
Characterization of moss ent-kaurene oxidase (CYP701B1) using a highly purified preparation
J. Biochem.
163
69-76
2018
Physcomitrium patens, Physcomitrium patens (F2ZAE0)
brenda
Yamamura, Y.; Taguchi, Y.; Ichitani, K.; Umebara, I.; Ohshita, A.; Kurosaki, F.; Lee, J.B.
Characterization of ent-kaurene synthase and kaurene oxidase involved in gibberellin biosynthesis from Scoparia dulcis
J. Nat. Med.
72
456-463
2018
Scoparia dulcis (A0A0N7H9X2), Scoparia dulcis (A0A0N9YKX0), Scoparia dulcis
brenda
Itoh, H.; Tatsumi, T.; Sakamoto, T.; Otomo, K.; Toyomasu, T.; Kitano, H.; Ashikari, M.; Ichihara, S.; Matsuoka, M.
A rice semi-dwarf gene, Tan-Ginbozu (D35), encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase
Plant Mol. Biol.
54
533-547
2004
Oryza sativa Japonica Group (Q5Z5R4)
brenda
Mafu, S.; Jia, M.; Zi, J.; Morrone, D.; Wu, Y.; Xu, M.; Hillwig, M.L.; Peters, R.J.
Probing the promiscuity of ent-kaurene oxidases via combinatorial biosynthesis
Proc. Natl. Acad. Sci. USA
113
2526-2531
2016
Arabidopsis thaliana (Q93ZB2), Oryza sativa Japonica Group (Q5Z5R4)
brenda
Su, P.; Tong, Y.; Cheng, Q.; Hu, Y.; Zhang, M.; Yang, J.; Teng, Z.; Gao, W.; Huang, L.
Functional characterization of ent-copalyl diphosphate synthase, kaurene synthase and kaurene oxidase in the Salvia miltiorrhiza gibberellin biosynthetic pathway
Sci. Rep.
6
23057
2016
Salvia miltiorrhiza (A0A0G2RKY1), Salvia miltiorrhiza
brenda
Zhao, D.; Shen, Y.; Shi, Y.; Shi, X.; Qiao, Q.; Zi, S.; Zhao, E.; Yu, D.; Kennelly, E.
Probing the transcriptome of Aconitum carmichaelii reveals the candidate genes associated with the biosynthesis of the toxic aconitine-type C19-diterpenoid alkaloids
Phytochemistry
152
113-124
2018
Aconitum carmichaelii
brenda
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