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EC Tree
IUBMB Comments A cytochrome P-450 (heme-thiolate) protein isolated from rice, Oryza sativa.
The expected taxonomic range for this enzyme is: Oryza sativa
Synonyms
CYP701A8, KOL4,
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CYP701A8
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9beta-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2 = 9beta-pimara-7,15-diene-3beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
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9beta-pimara7,15-diene,[reduced NADPHbetahemoprotein reductase]:oxygen oxidoreductase (9beta-pimara-7,15-diene-3beta-ol forming)
A cytochrome P-450 (heme-thiolate) protein isolated from rice, Oryza sativa.
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9beta-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
9beta-pimara-7,15-diene-3beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
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0.027
9beta-pimara-7,15-diene
pH 7.5, 32°C
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1.4
9beta-pimara-7,15-diene
pH 7.5, 32°C
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52
9beta-pimara-7,15-diene
pH 7.5, 32°C
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UniProt
brenda
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physiological function
enzyme efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids, reaction of EC 1.14.13.191, intermediates in biosynthesis of the oryzalexin and phytocassane families of rice antifungal phytoalexins
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C7018_ORYSJ
510
1
56965
Swiss-Prot
Secretory Pathway (Reliability: 1 )
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Kitaoka, N.; Wu, Y.; Xu, M.; Peters, R.J.
Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis
Appl. Microbiol. Biotechnol.
99
7549-7558
2015
Oryza sativa (Q0DBF4)
brenda
Wang, Q.; Hillwig, M.L.; Wu, Y.; Peters, R.J.
CYP701A8 a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism
Plant Physiol.
158
1418-1425
2012
Oryza sativa (Q0DBF4)
brenda
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