We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments A cytochrome P-450 (heme-thiolate) protein. The enzyme from rice (Oryza sativa) is involved in the biosynthesis of the phytoalexin momilactone. It also acts similarly on 9beta-stemod-13(17)-ene.
The expected taxonomic range for this enzyme is: Oryza sativa
Synonyms
cyp99a3,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CYP99A3
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
9beta-pimara-7,15-dien-19-al + O2 + [reduced NADPH-hemoprotein reductase] = 9beta-pimara-7,15-dien-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
(1c)
-
-
9beta-pimara-7,15-dien-19-ol + O2 + [reduced NADPH-hemoprotein reductase] = 9beta-pimara-7,15-dien-19-al + [oxidized NADPH-hemoprotein reductase] + 2 H2O
(1b)
-
-
9beta-pimara-7,15-diene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase] = 9beta-pimara-7,15-dien-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction
-
-
9beta-pimara-7,15-diene + O2 + [reduced NADPH-hemoprotein reductase] = 9beta-pimara-7,15-dien-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
(1a)
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
9beta-pimara-7,15-diene,[reduced NADPH-hemoprotein reductase]:oxygen 19-oxidoreductase
A cytochrome P-450 (heme-thiolate) protein. The enzyme from rice (Oryza sativa) is involved in the biosynthesis of the phytoalexin momilactone. It also acts similarly on 9beta-stemod-13(17)-ene.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
9beta-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
3beta-hydroxy-9beta-pimara-7,15-dien-19,6beta-olide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
syn-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
syn-pimaradien-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
syn-pimaradien-19-al + [reduced NADPH-hemoprotein reductase] + O2
syn-pimaradien-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
syn-pimaradien-19-ol + [reduced NADPH-hemoprotein reductase] + O2
syn-pimaradien-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
syn-stemod-13(17)-ene + [reduced NADPH-hemoprotein reductase] + O2
syn-stemoden-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
-
?
syn-stemoden-19-al + [reduced NADPH-hemoprotein reductase] + O2
syn-stemoden-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
-
?
syn-stemoden-19-ol + [reduced NADPH-hemoprotein reductase] + O2
syn-stemoden-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
about 4fold lower catalytic efficiency than with syn-pimaradiene, oxidation occurs at C19
-
?
additional information
?
-
additional information
?
-
CYP99A3 catalyzes consecutive oxidations of the C19 methyl group of the momilactone precursor syn-pimara-7,15-diene to form, sequentially, syn-pimaradien-19-ol, syn-pimaradien-19-al, and syn-pimaradien-19-oate
-
?
additional information
?
-
-
CYP99A3 catalyzes consecutive oxidations of the C19 methyl group of the momilactone precursor syn-pimara-7,15-diene to form, sequentially, syn-pimaradien-19-ol, syn-pimaradien-19-al, and syn-pimaradien-19-oate
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.002
syn-pimara-7,15-diene
pH and temperature not specified in the publication
0.009
syn-stemod-13(17)-ene
pH and temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
46
syn-pimara-7,15-diene
pH and temperature not specified in the publication
49
syn-stemod-13(17)-ene
pH and temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
23000
syn-pimara-7,15-diene
pH and temperature not specified in the publication
5000
syn-stemod-13(17)-ene
pH and temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
cultivar Nipponbare
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
malfunction
-
the double knockdown of CYP99A2 and CYP99A3 specifically suppresses the elicitor-inducible production of momilactones
physiological function
CYP99A3 acts as a multifunctional diterpene oxidase in momilactone biosynthesis
physiological function
-
CYP99A3 is involved in momilactone biosynthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
C99A3_ORYSJ
502
2
55892
Swiss-Prot
Chloroplast (Reliability: 5 )
A0A2I0B5N9_9ASPA
57
0
6385
TrEMBL
other Location (Reliability: 3 )
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli C41 cells
expressed in Escherichia coli Rosetta (DE3) cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Shimura, K.; Okada, A.; Okada, K.; Jikumaru, Y.; Ko, K.W.; Toyomasu, T.; Sassa, T.; Hasegawa, M.; Kodama, O.; Shibuya, N.; Koga, J.; Nojiri, H.; Yamane, H.
Identification of a biosynthetic gene cluster in rice for momilactones
J. Biol. Chem.
282
34013-34018
2007
Oryza sativa
brenda
Wang, Q.; Hillwig, M.; Peters, R.
CYP99A3: Functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice
Plant J.
65
87-95
2011
Oryza sativa (Q0JF01), Oryza sativa
brenda
Select items on the left to see more content.
html completed