The enzyme, characterized from the bacterium Pseudomonas sp. CBB1, is part of the bacterial C-8 oxidation-based caffeine degradation pathway. The product decomposes spontaneously to a racemic mixture of 3,6,8-trimethylallantoin. The enzyme shows no acitivity with urate. cf. EC 1.14.13.113, FAD-dependent urate hydroxylase.
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The expected taxonomic range for this enzyme is: Pseudomonas sp.
The enzyme, characterized from the bacterium Pseudomonas sp. CBB1, is part of the bacterial C-8 oxidation-based caffeine degradation pathway. The product decomposes spontaneously to a racemic mixture of 3,6,8-trimethylallantoin. The enzyme shows no acitivity with urate. cf. EC 1.14.13.113, FAD-dependent urate hydroxylase.
genes involved in the caffeine C-8 oxidation pathway are located in a 25.2-kb genomic DNA fragment of CBB1, including cdhABC (coding for caffeine dehydrogenase) and tmuM (coding for TmuM), Physical map of genes for caffeine transformation in a 25.2-kb gene cluster in Pseudomonas sp. strain CBB1, overview
the enzyme takes part in the caffeine C-8 oxidation pathway as the second enzyme, a NADH-dependent trimethyluric acid monooxygenase that catalyzes the conversion of trimethylurate to 1,3,7-trimethyl-5-hydroxyisourate. This product spontaneously decomposes to racemic 3,6,8-trimethylallantoin
two distinct caffeine metabolic pathways in bacteria: N-demethylation and C-8 oxidation, the NADH-dependent trimethyluric acid monooxygenase is involved in the latter catalyzing the second step of the C-8 oxidation pathway, overview
homology models of trimethyluric acid monooxygenase against uric acid oxidase HpxO (which catalyzes uric acid to 5-hydroxyisourate) reveal a much bigger and hydrophobic cavity to accommodate the larger substrates
homology models of trimethyluric acid monooxygenase against uric acid oxidase HpxO (which catalyzes uric acid to 5-hydroxyisourate) reveal a much bigger and hydrophobic cavity to accommodate the larger substrates
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gene tmuM, DNA and amino acid sequence determination and analysis, genes involved in the caffeine C-8 oxidation pathway are located in a 25.2-kb genomic DNA fragment of CBB1, including cdhABC (coding for caffeine dehydrogenase) and tmuM (coding for TmuM)
gene tmuM, genes involved in the caffeine C-8 oxidation pathway are located in a 25.2-kb genomic DNA fragment of CBB1, including cdhABC (coding for caffeine dehydrogenase) and tmuM (coding for TmuM)
treatment of coffee waste with caffeine-degrading microorganisms (either wild type or recombinant) may transform the waste into a valuable by-product, rather than a waste stream, because a caffeine concentration in the waste greater than 1% makes it unsuitable as animal feed or as a biofuel feedstock
Delineation of the caffeine C-8 oxidation pathway in Pseudomonas sp. strain CBB1 via characterization of a new trimethyluric acid monooxygenase and genes involved in trimethyluric acid metabolism
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1.14.13.212 1,3,7-trimethyluric acid 5-monooxygenase
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