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2,3-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
2,3-dichlorophenol + NADPH + O2
3,4-dichlorocatechol + NADP+ + H2O
Soil bacterium
-
113% of the activity with 2,4-dichlorophenol
-
?
2,3-dichlorophenol + NADPH + O2
?
2,4,5-trichlorophenol + NADPH + O2
?
2,4,6-trichlorophenol + NADPH + O2
?
-
-
-
?
2,4-dibromophenol + NADPH + O2
?
2,4-dichlorophenol + NADH + H+ + O2
3,5-dichlorocatechol + NAD+ + H2O
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
2,5-dichlorophenol + NADPH + O2
3,6-dichlorocatechol + NADP+ + H2O
Soil bacterium
-
103% of the activity with 2,4-dichlorophenol
-
?
2,5-dichlorophenol + NADPH + O2
?
-
-
-
?
2,5-dichlorophenol + NADPH + O2
? + NADP+ + H2O
Soil bacterium
-
178% of the activity with 2,4-dichlorophenol
-
?
2,6-dichlorophenol + NADPH + O2
?
-
-
-
?
2-bromo-4-chlorophenol + NADPH + O2
3-bromo-5-chlorocatechol + NADP+ + H2O
Soil bacterium
-
82% of the activity with 2,4-dichlorophenol
-
?
2-chloro-4-nitrophenol + NADPH + O2
?
-
-
-
?
2-chlorophenol + NADPH + O2
3-chlorocatechol + NADP+ + H2O
Soil bacterium
-
129% of the activity with 2,4-dichlorophenol
-
?
2-chlorophenol + NADPH + O2
?
2-cresol + NADPH + O2
?
-
incompletely metabolized
-
?
2-methylphenol + NADPH + O2
?
-
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
3,4-dichlorophenol + NADPH + O2
?
-
-
-
?
3,5-dichlorophenol + NADPH + O2
?
-
-
-
?
3-chlorophenol + NADPH + O2
4-chlorocatechol + NADP+ + H2O
Soil bacterium
-
230% of the activity with 2,4-dichlorophenol
-
?
3-chlorophenol + NADPH + O2
?
-
-
-
?
4-bromo-2-chlorophenol + NADPH + O2
5-bromo-3-chlorocatechol + NADP+ + H2O
Soil bacterium
-
86% of the activity with 2,4-dichlorophenol
-
?
4-bromophenol + NADPH + O2
?
4-chloro-2-methylphenol + NADPH + O2
5-chloro-3-methylcatechol + NADP+ + H2O
Soil bacterium
-
95% of the activity with 2,4-dichlorophenol
-
?
4-chloro-2-methylphenol + NADPH + O2
?
4-chloro-2-nitrophenol + NADPH + O2
?
-
-
-
?
4-chlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
4-chlorophenol + NADPH + O2
?
additional information
?
-
2,3-dichlorophenol + NADPH + H+ + O2

? + NADP+ + H2O
26% activity compared to 2,4-dichlorophenol as substrate
-
?
2,3-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
26% activity compared to 2,4-dichlorophenol as substrate
-
?
2,3-dichlorophenol + NADPH + O2

?
-
-
-
?
2,3-dichlorophenol + NADPH + O2
?
Proteobacteria S1
-
-
-
?
2,4,5-trichlorophenol + NADPH + O2

?
-
-
-
?
2,4,5-trichlorophenol + NADPH + O2
?
-
-
-
?
2,4,5-trichlorophenol + NADPH + O2
?
-
-
-
?
2,4,5-trichlorophenol + NADPH + O2
?
Proteobacteria S1
-
-
-
?
2,4-dibromophenol + NADPH + O2

?
-
-
-
?
2,4-dibromophenol + NADPH + O2
?
-
-
-
?
2,4-dibromophenol + NADPH + O2
?
-
-
-
?
2,4-dibromophenol + NADPH + O2
?
Proteobacteria S1
-
-
-
?
2,4-dichlorophenol + NADH + H+ + O2

3,5-dichlorocatechol + NAD+ + H2O
-
-
-
r
2,4-dichlorophenol + NADH + H+ + O2
3,5-dichlorocatechol + NAD+ + H2O
-
-
-
r
2,4-dichlorophenol + NADPH + H+ + O2

3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
Soil bacterium
-
-
-
?
2,4-dichlorophenol + NADPH + O2

3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
Proteobacteria S1
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
2-chlorophenol + NADPH + O2

?
-
incompletely metabolized
-
?
2-chlorophenol + NADPH + O2
?
-
incompletely metabolized
-
?
2-chlorophenol + NADPH + O2
?
-
incompletely metabolized
-
?
2-chlorophenol + NADPH + O2
?
-
-
-
?
3,4-dichlorophenol + NADPH + H+ + O2

? + NADP+ + H2O
30% activity compared to 2,4-dichlorophenol as substrate
-
?
3,4-dichlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
30% activity compared to 2,4-dichlorophenol as substrate
-
?
4-bromophenol + NADPH + O2

?
-
incompletely metabolized
-
?
4-bromophenol + NADPH + O2
?
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2

?
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
Proteobacteria S1
-
-
-
?
4-chlorophenol + NADPH + H+ + O2

? + NADP+ + H2O
68% activity compared to 2,4-dichlorophenol as substrate
-
?
4-chlorophenol + NADPH + H+ + O2
? + NADP+ + H2O
68% activity compared to 2,4-dichlorophenol as substrate
-
?
4-chlorophenol + NADPH + O2

5-chlorocatechol + NADP+ + H2O
-
-
-
?
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
-
-
-
?
4-chlorophenol + NADPH + O2

?
-
-
-
?
4-chlorophenol + NADPH + O2
?
-
-
-
?
4-chlorophenol + NADPH + O2
?
-
-
-
?
additional information

?
-
-
pseudosubstrates evoke oxidation of NAD(P)H and oxygen consumption without themselves undergoing hydroxylation, the product is hydrogen peroxide: 2,5-dichlorophenol, 2,6-dichlorophenol, 3,4-dichlorophenol, 2, 4,5-trichlorophenol
-
?
additional information
?
-
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
-
?
additional information
?
-
-
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
-
?
additional information
?
-
the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview
-
?
additional information
?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
-
?
additional information
?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
4-chloro-2-methylphenol + NADPH + O2
?
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
additional information
?
-
2,4-dichlorophenol + NADPH + H+ + O2

3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
-
?
2,4-dichlorophenol + NADPH + H+ + O2
3,5-dichlorocatechol + NADP+ + H2O
best substrate
-
-
?
2,4-dichlorophenol + NADPH + O2

3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
Proteobacteria S1
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
2,4-dichlorophenol + NADPH + O2
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2

?
-
-
-
-
?
4-chloro-2-methylphenol + NADPH + O2
?
-
-
-
-
?
4-chlorophenol + NADPH + O2

5-chlorocatechol + NADP+ + H2O
-
-
-
-
?
4-chlorophenol + NADPH + O2
5-chlorocatechol + NADP+ + H2O
-
-
-
-
?
additional information

?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
-
-
?
additional information
?
-
-
the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Beadle, C.A.; Smith, A.R.W.
The purification and properties of 2,4-dichlorophenol hydroxylase from a strain of Acinetobacter species
Eur. J. Biochem.
123
323-332
1982
Acinetobacter sp.
brenda
Liu, T.; Chapman, P.J.
Purification and properties of a plasmid-encoded 2,4-dichlorophenol hydroxylase
FEBS Lett.
173
314-318
1984
Cupriavidus necator, Cupriavidus necator JMP 134-1
brenda
Radjendirane, V.; Bhat, M.A.; Vaidyanathan, C.S.
Affinity purification and characterization of 2,4-dichlorophenol hydroxylase from Pseudomonas cepacia [published erratum appears in Arch Biochem Biophys 1992 Jul;296(1):354]
Arch. Biochem. Biophys.
288
169-176
1991
Burkholderia cepacia
brenda
Farhana, L.; Fulthorpe, R.R.; Harbour, C.; New, P.B.
Monoclonal antibodies to 2,4-dichlorophenol hydroxylase as probes for the 2,4-D-degradative phenotype
Can. J. Microbiol.
44
920-928
1998
Cupriavidus necator, Variovorax paradoxus, Burkholderia cepacia, Burkholderia mallei, Burkholderia sp., Sphingomonas paucimobilis, Pseudomonas putida, Rhodoferax fermentans, Rhodopseudomonas palustris, Sphingomonas sp., Sphingomonas sp. B6-5, Rhodopseudomonas palustris M1, Burkholderia mallei TFD6, Sphingomonas paucimobilis 1443, Burkholderia sp. RASC, Sphingomonas sp. EML146, Variovorax paradoxus 2811P, Rhodoferax fermentans B6-9, Pseudomonas putida PaW85, Burkholderia sp. TFD2, Sphingomonas sp. TFD44, Cupriavidus necator JMP 134-1, Rhodoferax fermentans TFD23, Burkholderia cepacia DBO131, Cupriavidus necator TFD41, Sphingomonas sp. B6-10
brenda
Farhana, L.; New, P.B.
The 2,4-dichlorophenol hydroxylase of Alcaligenes eutrophus JMP134 is a homotetramer
Can. J. Microbiol.
43
202-205
1997
Cupriavidus necator, Cupriavidus necator JMP 134-1
brenda
Makdessi, K.; Lechner, U.
Purification and characterization of 2,4-dichlorophenol hydroxylase isolated from a bacterium of the alpha-2 subgroup of the Proteobacteria
FEMS Microbiol. Lett.
157
95-101
1997
Proteobacteria, Proteobacteria S1
brenda
Ledger, T.; Pieper, D.H.; Gonzalez, B.
Chlorophenol hydroxylases encoded by plasmid pJP4 differentially contribute to chlorophenoxyacetic acid degradation
Appl. Environ. Microbiol.
72
2783-2792
2006
Cupriavidus necator, Paraburkholderia xenovorans
brenda
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