Information on EC 22.214.171.124 - 3-(3-hydroxyphenyl)propanoate hydroxylasefor references in articles please use BRENDA:EC126.96.36.199
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A flavoprotein (FAD). This enzyme participates in a meta-cleavage pathway employed by the bacterium Escherichia coli for the degradation of various phenylpropanoid compounds.
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The enzyme appears in viruses and cellular organisms
3-(3-hydroxyphenyl)propionate hydroxylase, 3HPP hydroxylase, MhpA, more
(2E)-3-(3-hydroxyphenyl)prop-2-enoate + NADH + H+ + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD+
3-(3-hydroxyphenyl)propanoate + NADH + H+ + O2 = 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+
the His-Cys catalytic diad in free papain is fully protonated, -NH(+)/-SH. The experimental pKa of 8.62 for His159 imidazole in free papain is assigned to the -NH(+)/-SH and -N/-SH equilibrium
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