Information on EC 1.14.13.127 - 3-(3-hydroxyphenyl)propanoate hydroxylase

for references in articles please use BRENDA:EC1.14.13.127
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
1.14.13.127
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RECOMMENDED NAME
GeneOntology No.
3-(3-hydroxyphenyl)propanoate hydroxylase
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REACTION
REACTION DIAGRAM
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ORGANISM
UNIPROT
LITERATURE
(2E)-3-(3-hydroxyphenyl)prop-2-enoate + NADH + H+ + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD+
show the reaction diagram
(2)
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3-(3-hydroxyphenyl)propanoate + NADH + H+ + O2 = 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+
show the reaction diagram
the His-Cys catalytic diad in free papain is fully protonated, -NH(+)/-SH. The experimental pKa of 8.62 for His159 imidazole in free papain is assigned to the -NH(+)/-SH and  -N/-SH equilibrium
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-oxopent-4-enoate
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cinnamate and 3-hydroxycinnamate degradation to 2-oxopent-4-enoate
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3-phenylpropionate degradation
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Phenylalanine metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
3-(3-hydroxyphenyl)propanoate,NADH:oxygen oxidoreductase (2-hydroxylating)
A flavoprotein (FAD). This enzyme participates in a meta-cleavage pathway employed by the bacterium Escherichia coli for the degradation of various phenylpropanoid compounds.
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ORGANISM
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LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Escherichia coli
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Manually annotated by BRENDA team
no activity in Escherichia coli LW279
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Manually annotated by BRENDA team
no activity in Escherichia coli LW303
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
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LITERATURE
(Substrate)
COMMENTARY
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LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-(3-hydroxyphenyl)propanoate + NADH + H+ + O2
3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O
show the reaction diagram
additional information
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MhpA does not oxidize melilotate
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
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LITERATURE
62050
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calculated from amino acid sequence
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
growth with 3HCI induces the synthesis of enzyme MhpA
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Show AA Sequence (1021 entries)
Please use the Sequence Search for a specific query.
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.127)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)