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Information on EC 1.14.12.1 - anthranilate 1,2-dioxygenase (deaminating, decarboxylating)
for references in articles please use BRENDA:EC1.14.12.1
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EC Tree 1 Oxidoreductases
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
1.14.12.1 anthranilate 1,2-dioxygenase (deaminating, decarboxylating)
IUBMB Comments
Requires Fe2+.
The enzyme appears in viruses and cellular organisms
- 1.14.12.1
- catechol
-
two-subunit
-
rieske-type
-
davies
- 2-aminobenzoate
- putida
- benzoate
-
pulsed
- carbazole
- microwave
-
non-heme
-
carbazole-degradative
- fe
- caraa
- ferredoxin
- niger
-
multi-sequence
- acinetobacter
-
endor
- sphingomonas
showSynonyms
antdo, two-component anthranilate 1,2-dioxygenase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
anthranilate hydroxylase
-
-
-
-
anthranilic acid hydroxylase
-
-
-
-
anthranilic hydroxylase
-
-
-
-
two-component anthranilate 1,2-dioxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
anthranilate + NAD(P)H + H+ + O2 = catechol + CO2 + NAD(P)+ + NH3
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Deamination
-
-
-
-
decarboxylation
-
-
-
-
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
2-nitrobenzoate degradation II, anthranilate degradation I (aerobic)
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SYSTEMATIC NAME
IUBMB Comments
anthranilate,NAD(P)H:oxygen oxidoreductase (1,2-hydroxylating, deaminating, decarboxylating)
Requires Fe2+.
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CAS REGISTRY NUMBER
COMMENTARY
9059-17-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxybenzoic acid + NAD(P)H + O2 + H2O
?
-
Substrates: -
Products: -
Products: -
?
anthranilate + NADPH + H+ + O2
catechol + CO2 + NADP+ + NH3
-
Substrates: -
Products: -
Products: -
?
protocatechuic acid + NAD(P)H + O2 + H2O
?
-
Substrates: -
Products: -
Products: -
?
salicylic acid + NAD(P)H + O2 + H2O
?
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: salicylic acid and o-aminophenol are no substrates
Products: -
Products: -
?
anthranilic acid + alanine + O2
?
Substrates: -
Products: -
Products: -
?
anthranilic acid + alanine + O2
?
Burkholderia cepacia DBO1 / ATCC 29424
Substrates: -
Products: -
Products: -
?
anthranilic acid + alanine + O2
catechol + CO2 + NH3 + ?
Substrates: involved in the tryptophan degradation pathway
Products: -
Products: -
?
anthranilic acid + alanine + O2
catechol + CO2 + NH3 + ?
Burkholderia cepacia DBO1 / ATCC 29424
Substrates: involved in the tryptophan degradation pathway
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
Substrates: involved in the tryptophan degradation pathway
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
Burkholderia cepacia DBO1 / ATCC 29424
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
Burkholderia cepacia DBO1 / ATCC 29424
Substrates: involved in the tryptophan degradation pathway
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: initial step in aerobic degradation of aromatic compounds
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: initial step in aerobic degradation of aromatic compounds
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NADH + O2 + H2O
catechol + CO2 + NAD+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NADH + O2 + H2O
catechol + CO2 + NAD+ + NH3
-
Substrates: -
Products: -
Products: -
?
benzoate + NAD(P)H + O2 + H2O
benzoate dihydrodiol + ?
Substrates: poor substrate
Products: -
Products: -
?
benzoate + NAD(P)H + O2 + H2O
benzoate dihydrodiol + ?
Burkholderia cepacia DBO1 / ATCC 29424
Substrates: poor substrate
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
anthranilate + NADPH + H+ + O2
catechol + CO2 + NADP+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + alanine + O2
catechol + CO2 + NH3 + ?
Substrates: involved in the tryptophan degradation pathway
Products: -
Products: -
?
anthranilic acid + alanine + O2
catechol + CO2 + NH3 + ?
Burkholderia cepacia DBO1 / ATCC 29424
Substrates: involved in the tryptophan degradation pathway
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: initial step in aerobic degradation of aromatic compounds, enables growth on anthranilate as sole carbon source
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
Substrates: involved in the tryptophan degradation pathway
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
Burkholderia cepacia DBO1 / ATCC 29424
Substrates: involved in the tryptophan degradation pathway
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: initial step in aerobic degradation of aromatic compounds
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: initial step in aerobic degradation of aromatic compounds
Products: -
Products: -
?
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
-
Substrates: -
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Burkholderia cepacia DBO1 / ATCC 29424
Burkholderia cepacia DBO1 / ATCC 29424
large subunit; three component type of enzyme
SwissProt
brenda
Burkholderia cepacia DBO1 / ATCC 29424
small subunit; three component type of enzyme
SwissProt
brenda
Highest Expressing Human Cell Lines
Cell Line Links Show AA Sequence and Transmembrane Helices (1265 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
18900
AndAd, product of ORF4, calculated from deduced amino acid sequence
24000
-
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration
48000
AndAc, product of ORF3, calculated from deduced amino acid sequence
52000
-
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexamer
trimer
hexamer
-
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration
hexamer
-
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration
-
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
clustered antABC genes from ADP1 chromosome encodes anthranilate dioxygenase
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bundy, B.M.; Campbell, A.L.; Neidle, E.L.
Similarities between the antABC-encoded anthranilate dioxygenase and the benABC-encoded benzoate dioxygenase of Acinetobacter sp. strain ADP1
J. Bacteriol.
180
4466-4474
1998
Acinetobacter sp., Acinetobacter sp. ADP1, Pseudomonas sp.
brenda
Taniuchi, H.; Hatanaka, M.; Kuno, S.; Hayaishi, O.; Nakajima, M.; Kurihara, N.
Enzymatic formation of catechol from anthranilic acid
J. Biol. Chem.
239
2204-2211
1964
Pseudomonas fluorescens
brenda
Roenberg, S.L.; Hegeman, G.D.
Genetics of the mandelate pathway in Pseudomonas aeruginosa
J. Bacteriol.
108
1270-1276
1971
Pseudomonas aeruginosa
brenda
Wheels, M.L.
Regulation of the synthesis of enzymes of tryptophan dissimilation in Acinetobacter calcoaceticus
Arch. Mikrobiol.
87
1-9
1972
Acinetobacter calcoaceticus
brenda
Salcher, O.; Lingens, F.
Metabolism of tryptophan by Pseudomonas aureofaciens and its relationship to pyrrolnitrin biosynthesis
J. Gen. Microbiol.
121
465-471
1980
Pseudomonas chlororaphis subsp. aureofaciens
brenda
Macheroux, Peter; Kojiro, C.L.; Schopfer, L.M.; Chakraborty, S.; Massey, V.
F NMR studies on fluoroflavins and 8-fluoroflavoproteins
Biochemistry
29
2670-2679
1990
Cutaneotrichosporon cutaneum
brenda
Eby, D.M.; Beharry, Z.M.; Coulter, E.D.; Kurtz, D.M., Jr.; Neidle, E.L.
Characterization and evolution of anthranilate 1,2-dioxygenase from Acinetobacter sp. strain ADP1
J. Bacteriol.
183
109-118
2001
Acinetobacter sp., Acinetobacter sp. ADP1, Pseudomonas sp.
brenda
Beharry, Z.M.; Eby, D.M.; Coulter, E.D.; Viswanathan, R.; Neidle, E.L.; Phillips, R.S.; Kurtz, D.M., Jr.
Histidine ligand protonation and redox potential in the Rieske dioxygenases: Role of a conserved aspartate in anthranilate 1,2-Dioxygenase
Biochemistry
42
13625-13636
2003
Acinetobacter sp., Acinetobacter sp. ADP1
brenda
Chang, H.K.; Mohseni, P.; Zylstra, G.J.
Characterization and regulation of the genes for a novel anthranilate 1,2-dioxygenase from Burkholderia cepacia DBO1
J. Bacteriol.
185
5871-5881
2003
Burkholderia cepacia (Q84BZ2), Burkholderia cepacia (Q84BZ3), Burkholderia cepacia, Burkholderia cepacia DBO1 / ATCC 29424 (Q84BZ2), Burkholderia cepacia DBO1 / ATCC 29424 (Q84BZ3)
brenda
Urata, M.; Miyakoshi, M.; Kai, S.; Maeda, K.; Habe, H.; Omori, T.; Yamane, H.; Nojiri, H.
Transcriptional regulation of the ant operon, encoding two-component anthranilate 1,2-dioxygenase, on the carbazole-degradative plasmid pCAR1 of Pseudomonas resinovorans strain CA10
J. Bacteriol.
186
6815-6823
2004
Pseudomonas resinovorans, Pseudomonas resinovorans CA10
brenda
Iwaki, H.; Hasegawa, Y.
Degradation of 2-nitrobenzoate by Burkholderia terrae strain KU-15
Biosci. Biotechnol. Biochem.
71
145-151
2007
Paraburkholderia terrae, Paraburkholderia terrae KU-15
brenda
Balderas-Hernandez, V.; Trevino-Quintanilla, L.; Hernandez-Chavez, G.; Martinez, A.; Bolivar, F.; Gosset, G.
Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1
Microb. Cell Fact.
13
136
2014
Pseudomonas aeruginosa
brenda
EXTERNAL LINKS (specific for EC-Number 1.14.12.1)
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