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Requires Fe2+ and ascorbate. Found in the bacterium Streptomyces kanamyceticus where it is involved in the conversion of the aminoglycoside antibiotic kanamycin B to kanamycin A.
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The enzyme appears in viruses and cellular organisms
kanamycin b dioxygenase, more
kanamycin B + 2-oxoglutarate + O2 = 2'-dehydrokanamycin A + succinate + NH3 + CO2
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