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IUBMB Comments Requires Fe2+ Streptomyces kanamyceticus where it is involved in the conversion of the aminoglycoside antibiotic kanamycin B to kanamycin A.
Synonyms
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kanJ
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kanamycin B + 2-oxoglutarate + O2 = 2'-dehydrokanamycin A + succinate + NH3 + CO2
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MetaCyc
kanamycin biosynthesis
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kanamycin-B,2-oxoglutarate:oxygen oxidoreductase (deaminating, 2'-hydroxylating)
Requires Fe2+ and ascorbate. Found in the bacterium Streptomyces kanamyceticus where it is involved in the conversion of the aminoglycoside antibiotic kanamycin B to kanamycin A.
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kanamycin B + 2-oxoglutarate + O2
2'-dehydrokanamycin A + succinate + NH3 + CO2
kanamycin B + 2-oxoglutarate + O2
2'-dehydrokanamycin A + succinate + NH3 + CO2
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Substrates: -
r
kanamycin B + 2-oxoglutarate + O2
2'-dehydrokanamycin A + succinate + NH3 + CO2
Substrates: -
?
kanamycin B + 2-oxoglutarate + O2
2'-dehydrokanamycin A + succinate + NH3 + CO2
Substrates: -
?
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kanamycin B + 2-oxoglutarate + O2
2'-dehydrokanamycin A + succinate + NH3 + CO2
kanamycin B + 2-oxoglutarate + O2
2'-dehydrokanamycin A + succinate + NH3 + CO2
-
Substrates: -
r
kanamycin B + 2-oxoglutarate + O2
2'-dehydrokanamycin A + succinate + NH3 + CO2
Substrates: -
?
kanamycin B + 2-oxoglutarate + O2
2'-dehydrokanamycin A + succinate + NH3 + CO2
Substrates: -
?
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Fe2+
required
Fe2+
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the enzyme is dependent on exogenous ferrous ions, although residual activity is also observed in the absence of exogenous Fe2+
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2-oxoglutarate
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dependent on
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UniProt
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UniProt
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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KANJ_STRKN
285
0
31534
Swiss-Prot
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A0A158HIX5_9BURK
300
0
33860
TrEMBL
-
A0A158A0F3_9BURK
266
0
30143
TrEMBL
-
A0A5P9GTI6_9SPHN
252
0
28093
TrEMBL
-
A0A158K1R9_9BURK
261
0
29557
TrEMBL
-
A0A158EGC4_9BURK
266
0
30273
TrEMBL
-
A0A5E5ADD6_9BURK
294
0
32941
TrEMBL
-
A0A157ZEW4_9BURK
262
0
29669
TrEMBL
-
A0A157ZKE7_9BURK
262
0
29642
TrEMBL
-
A0A158IKJ1_9BURK
261
0
29614
TrEMBL
-
A0A1Y5TES5_9PROT
280
0
30581
TrEMBL
-
A0A5C6BRU6_9PLAN
269
0
29786
TrEMBL
-
A0A157ZXV7_9BURK
266
0
30044
TrEMBL
-
A0A2Z4AIT7_9BACT
295
0
33148
TrEMBL
-
A0A5B9P6A3_9BACT
262
0
29674
TrEMBL
-
A0A2U3I5P8_9BURK
266
0
29880
TrEMBL
-
A0A0N0XJ10_9NEIS
261
0
29507
TrEMBL
-
A0A158IEI9_9BURK
261
0
29734
TrEMBL
-
A0A517NW60_9BACT
262
0
29487
TrEMBL
-
A0A2Z4ACB1_9BACT
270
0
31585
TrEMBL
-
A0A6J5FV94_9BURK
263
0
29692
TrEMBL
-
A0A658QZR6_9BURK
266
0
30121
TrEMBL
-
A0A157ZI75_9BURK
266
0
30013
TrEMBL
-
A0A220W7E0_9SPHN
295
0
32986
TrEMBL
-
A0A0Q9YGT1_9COXI
265
0
30703
TrEMBL
-
A0A2Z2NSY1_9GAMM
742
0
82901
TrEMBL
-
A0A517TA66_9PLAN
293
0
32865
TrEMBL
-
A0A157ZDL1_9BURK
266
0
29868
TrEMBL
-
A0A5P9GPU5_9SPHN
287
0
31892
TrEMBL
-
A0A1V5FMA4_9BACT
264
0
29496
TrEMBL
-
A0A158IYD6_9BURK
261
0
29893
TrEMBL
-
A0A2Z2NRJ4_9GAMM
624
0
70244
TrEMBL
-
A0A2Z4AH92_9BACT
260
0
30633
TrEMBL
-
A0A245ZE82_9SPHN
275
0
30011
TrEMBL
-
A0A517ZUP1_9PLAN
269
0
29627
TrEMBL
-
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nickel-affinity column chromatography
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expressed in Escherichia coli DH5alpha cells
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Sucipto, H.; Kudo, F.; Eguchi, T.
The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the alpha-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK
Angew. Chem. Int. Ed. Engl.
51
3428-3431
2012
Streptomyces kanamyceticus
brenda
Gao, W.; Wu, Z.; Sun, J.; Ni, X.; Xia, H.
Modulation of kanamycin B and kanamycin A biosynthesis in Streptomyces kanamyceticus via metabolic engineering
PLoS ONE
12
e0181971
2017
Streptomyces kanamyceticus (Q6L732), Streptomyces kanamyceticus CG305 (Q6L732)
brenda
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