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EC Tree
The enzyme appears in viruses and cellular organisms
Reaction Schemes
cis-1,4-polyisoprene
+
n
=
tetra-C20 and higher oligo-isoprenoids with aldehyde and keto end groups
Synonyms
latex clearing protein, Lcp, RoxB, rubber oxygenase B,
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cis-1,4-polyisoprene + n O2 = tetra-C20 and higher oligo-isoprenoids with aldehyde and keto end groups
cis-1,4-polyisoprene + n O2 = tetra-C20 and higher oligo-isoprenoids with aldehyde and keto end groups
endo-cleavage
cis-1,4-polyisoprene + n O2 = tetra-C20 and higher oligo-isoprenoids with aldehyde and keto end groups
-
-
-
-
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cis-1,4-polyisoprene:oxygen dioxygenase [oligo-isoprenoids with aldehyde and keto end groups forming]
-
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cis-1,4-polyisoprene + n O2
?
additional information
?
-
no substrates: squalene (1,4-trans-isoprenoid), carotenoids, or alpha-tocopherol
-
-
?
cis-1,4-polyisoprene + n O2
?
-
-
-
?
cis-1,4-polyisoprene + n O2
?
-
-
-
?
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additional information
absence of covalently attached heme groups
-
heme
presence of 2 c-type hemes, presence of two c-type heme binding motifs (C192HAC195H and C391ASC394H)
heme
presence of characteristic alpha-, beta- and gamma- (Soret)-bands at 562 nm, 532 nm, and 430 nm in the visible spectrum after chemical reduction. Purified Lcp as isolated contains an oxidized heme-Fe3+ that is free of bound dioxygen
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Iron
presence of one atom of iron per Lcp molecule. Purified Lcp as isolated contains an oxidized heme-Fe3+ that is free of bound dioxygen
additional information
no presence of copper
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alpha-tocopherol
1 mM, 40% inhibition
diethyl thiocarbamate
10 mM, 56% inhibition
dithiothreitol
strong inhibition
squalene
1 mM, 10% inhibition
Triton X-100
0.1%, no residual activity
Tween 20
0.1%, no residual activity
Diethyl dithiocarbamate
1 mM, 50% inhibition
Diethyl dithiocarbamate
-
imidazole
1 mM, 35% inhibition
SDS
0.1%, no residual activity
additional information
poor inhibition by sodium citrate, EDTA, ethyl xanthogenate, 4,5-dihydroxybenzene-1,3-disulfonate (tiron), 1,10-phenanthroline, or potassium ethyl 2,2'-bipyridine
-
additional information
not inhibitory: disodium 4,5-dihydroxy-1,3-benzenedisulfonate, potassium ethylxanthogenate, or phenanthroline
-
additional information
not inhibitory: EDTA up to 10 mM, carbon monoxide
-
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6 - 8
in potassium phosphate buffer or in HEPES buffer. Other buffers result in only poor activities
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UniProt
brenda
-
UniProt
brenda
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physiological function
synergistic effect of the simultaneous presence of RoxAXsp and RoxBXsp on the efficiency of polyisoprene cleavage
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A0A1S6Q8F9_9XANT
681
0
73751
TrEMBL
-
LCP_STRK3
Streptomyces sp. (strain K30)
407
0
44006
Swiss-Prot
-
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?
x * 48000, SDS-PAGE, x * 43300, calculated
?
x * 73800, calculated for precursor protein, x * 70300, calculated for mature protein, x * 70300, SDS-PAGE
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proteolytic modification
calculated molecular mass is 73800 Da for precursor protein, and 70300 Da for mature protein
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2
22 h, 5% loss of activity
22
22 h, 70% loss of activity
37
22 h, 80% loss of activity
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from a recombinant Xanthomonas sp. 35Y RoxA deletion mutant
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expression inXanthomonas sp. strain 35Y
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expression is upregulated during growth on polyisoprene
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degradation
enzyme exerts a synergistic effect on the efficiency of polyisoprene cleavage by rubber oxidase RoxA, EC 1.13.11.85
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Birke, J.; Jendrossek, D.
Rubber oxygenase and latex clearing protein cleave rubber to different products and use different cleavage mechanisms
Appl. Environ. Microbiol.
80
5012-5020
2014
Streptomyces sp. K30 (Q3L8N0)
brenda
Birke, J.; Röther, W.; Jendrossek, D.
Latex clearing protein (Lcp) of Streptomyces sp. strain K30 is a b-type cytochrome and differs from rubber oxygenase A (RoxA) in its biophysical properties
Appl. Environ. Microbiol.
81
3793-3799
2015
Streptomyces sp. K30 (Q3L8N0)
brenda
Birke, J.; Röther, W.; Jendrossek, D.
RoxB is a novel type of rubber oxygenase that combines properties of rubber oxygenase RoxA and latex clearing protein (Lcp)
Appl. Environ. Microbiol.
83
e00721
2017
Xanthomonas sp. (A0A1S6Q8F9)
brenda
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1.13.99.B1 exo-cleaving rubber dioxygenase
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