Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 1.13.12.24 - calcium-regulated photoprotein

for references in articles please use BRENDA:EC1.13.12.24
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
Ca2+-regulated photoproteins are found in a variety of bioluminescent marine organisms, mostly coelenterates, and are responsible for their light emission. The best studied enzyme is from the jellyfish Aequorea victoria. The enzyme tightly binds the imidazolopyrazinone derivative coelenterazine, which is then peroxidized by oxygen. The hydroperoxide is stably bound until three Ca2+ ions bind to the protein, inducing a structural change that results in the formation of a 1,2-dioxetan-3-one ring, followed by decarboxylation and generation of a protein-bound coelenteramide in an excited state. The calcium-bound protein-product complex is known as a blue fluorescent protein. In vivo the energy is transferred to a green fluorescent protein (GFP) by Forster resonance energy transfer. In vitro, in the absence of GFP, coelenteramide emits a photon of blue light while returning to its ground state.
Specify your search results
Select one or more organisms in this record: ?
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The expected taxonomic range for this enzyme is: Eumetazoa
Reaction Schemes
hide(Overall reactions are displayed. Show all >>)
[apoaequorin]
+
+
+
3
=
[excited state blue fluorescent protein]
+
Synonyms
obelin, blue fluorescent protein, mnemiopsin, berovin, mitrocomin, mnemiopsin 2, ca2+-regulated photoprotein, calcium-regulated photoprotein, mnemiopsin 1, halistaurin, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[aequorin] + 3 Ca2+ = [aequorin] 1,2-dioxetan-3-one
show the reaction diagram
(1c)
-
-
-
[aequorin] 1,2-dioxetan-3-one = [excited state blue fluorescent protein] + CO2
show the reaction diagram
(1d)
-
-
-
[apoaequorin containing coelenterazine] + O2 = [aequorin]
show the reaction diagram
(1b)
-
-
-
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ = [excited state blue fluorescent protein] + CO2
show the reaction diagram
overall reaction
-
-
-
[apoaequorin] + coelenterazine = [apoaequorin containing coelenterazine]
show the reaction diagram
(1a)
-
-
-
Select items on the left to see more content.