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IUBMB Comments Requires Fe2+
Reaction Schemes
[(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate
+
=
+
Synonyms tmpB ,
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tmpB
-
-
-
-
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[(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + O2 = glycine betaine + phosphate
-
-
-
-
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MetaCyc
(2-trimethylamino)ethylphosphonate degradation
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[(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate:oxygen 1R-oxidoreductase (glycine-betaine-forming)
Requires Fe2+. This bacterial enzyme is involved in a degradation pathway for [2-(trimethylamino)ethyl]phosphonate.
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[(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + O2
glycine betaine + phosphate
Substrates: -
?
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[(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + O2
glycine betaine + phosphate
Substrates: -
?
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Iron
the enzyme uses a mixed-valent diiron cofactor
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-
UniProt
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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A0A4V8H040_9RHOB
196
0
22202
TrEMBL
-
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in complex with Fe2+ and [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate, hanging drop vapor diffusion method, using 0.2 M CaCl2, 0.1 M sodium HEPES (pH 7.5), and 27-33% polyethylene glycol 4000
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Ni-NTA column chromatography and Sephadex S-200 gel filtration
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expressed in Escherichia coli BL21(DE3) cells
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Rajakovich, L.; Pandelia, M.; Mitchell, A.; Chang, W.; Zhang, B.; Boal, A.; Krebs, C.; Bollinger, J.
A new microbial pathway for organophosphonate degradation catalyzed by two previously misannotated non-heme-iron oxygenases
Biochemistry
58
1627-1647
2019
Leisingera caerulea (A0A4V8H040)
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