Contains Fe(II). The enzyme, charcterised from the bacteria Pseudomonas putida and Pseudomonas nitroreducens, catalyses the epoxidation of the double bond in the side chain of isoeugenol, followed by a second oxygenation and cleavage of the side chain in the form of acetaldehyde.
Contains Fe(II). The enzyme, charcterised from the bacteria Pseudomonas putida and Pseudomonas nitroreducens, catalyses the epoxidation of the double bond in the side chain of isoeugenol, followed by a second oxygenation and cleavage of the side chain in the form of acetaldehyde.
oxidative cleavage of isoeugenol by Iem is catalyzed via a monooxygenation reaction, and incorporation of the oxygen atom from O2 into vanillin is preferred over incorporation from water. Iem exhibits no activity toward any other of the phenylpropanoid and styrene compounds tested
enzyme catalyzes the initial step of isoeugenol degradation, the oxidative cleavage of the side chain double-bond of isoeugenol, to form vanillin. Enzyme catalyzes the incorporation of an oxygen atom from either molecular oxygen or water into vanillin
enzyme catalyzes the initial step of isoeugenol degradation, the oxidative cleavage of the side chain double-bond of isoeugenol, to form vanillin. Enzyme catalyzes the incorporation of an oxygen atom from either molecular oxygen or water into vanillin
strain is able to produce high amounts of vanillin when grown in the presence of isoeugenol, and is also capable of growing on isoeugenol as the sole carbon source. In the presence of isoeugenol, a growing culture produces 0.61 g/l vanillin (molar yield of 12.4%), whereas cell free extracts result in 0.9 g/l vanillin (molar yield of 14%)
strain is able to produce high amounts of vanillin when grown in the presence of isoeugenol, and is also capable of growing on isoeugenol as the sole carbon source. In the presence of isoeugenol, a growing culture produces 0.61 g/l vanillin (molar yield of 12.4%), whereas cell free extracts result in 0.9 g/l vanillin (molar yield of 14%)
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcription of Iem is dependent on the amounts of isoeugenol and the positive regulatory protein IemR. Isoeugenol is the best inducer of Iem, followed by trans-anethole, which induces Iem to 58% of the transcription level observed for isoeugenol. Overproduction of regulatory protein IemR in Escherichia coli increases the transcription of Iem up to 96fold, even in the absence of isoeugenol
the vanillin producing activity is induced by presence of isoeugenol. Under the optimized reaction conditions, Pseudomonas putida cells produce 16.1 g/l vanillin from 150 mM isoeugenol, with a molar conversion yield of 71% at 20 °C after a 24-h incubation in the presence of 10% (v/v) dimethyl sulfoxide
Escherichia coli cells expressing isoeugenol monooxygenase produce 28.3 g vanillin/l from 230 mM isoeugenol, with a molar conversion yield of 81% at 20°C after 6 h. No accumulation of undesired by-products, such as vanillic acid or acetaldehyde, is observed
upon expression of mutant F281Q in Escherichia coli and employing sol-gel chitosan membrane for removing the produced vanillin from the biotransformation system, under the optimal conditions (pH 8.5, 30°C, 180 rpm, 0.5 g wet cells, 0.1 g chitosan membrane), vanillin concentrations reach 4.5 g/l after about 40 h
Efficient biotransformation of isoeugenol to vanillin in recombinant strains of Escherichia coli by using engineered isoeugenol monooxygenase and sol-gel chitosan membrane
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Enzyme Nomenclature
1.13.11.88 isoeugenol monooxygenase
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