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EC Tree
IUBMB Comments A thiol-dependent enzyme isolated from rat and rabbit. Unlike EC 1.13.11.63, beta-carotene-15,15'-dioxygenase, it is not active towards beta-carotene. The secondary product has not been characterized, but may be (3E,5E)-7-hydroxy-6-methylhepta-3,5-dien-2-one.
The enzyme appears in viruses and cellular organisms
Synonyms
CarT, torulene oxygenase,
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8'-apo-beta-carotenol + O2 = 14'-apo-beta-carotenal + an uncharacterized product
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8'-apo-beta-carotenol:O2 oxidoreductase (14',13'-cleaving)
A thiol-dependent enzyme isolated from rat and rabbit. Unlike EC 1.13.11.63, beta-carotene-15,15'-dioxygenase, it is not active towards beta-carotene. The secondary product has not been characterized, but may be (3E,5E)-7-hydroxy-6-methylhepta-3,5-dien-2-one.
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8'-apo-beta-carotenol + O2
14'-apo-beta-carotenal + ?
beta-apo-10'-carotenal + O2
retinal + ?
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?
beta-apo-8'-carotenal + O2
(2E,4E,6E,8E,10E)-5,9-dimethyl-11-(2,6,6-trimethylcyclohex-1-en-1-yl)undeca-2,4,6,8,10-pentaenal + (2E,4E)-2-methylhepta-2,4-dienedial
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?
beta-apo-8'-lycopenal + O2
crocetindial + ?
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main product
?
lycopene + O2
?
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eccentric cleavage close to the end of lycopene
?
torulene + O2
beta-apo-4'-carotenal + 3-methylbut-2-enal
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?
additional information
?
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enzyme cleaves at the third double bond from the uncycled tail of carotenoids with preference for non-oxygenated tails
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?
8'-apo-beta-carotenol + O2
14'-apo-beta-carotenal + ?
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?
8'-apo-beta-carotenol + O2
14'-apo-beta-carotenal + ?
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?
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1,10-phenanthroline
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complete inhibition at 1 mM
1,10-phenanthroline
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complete inhibition at 1 mM
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detergent
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detergent is necessary for solubilizing substrates, maximal activity with 0.5% CHAPS or 0.5% sodium cholate
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detergent
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detergent is necessary for solubilizing substrates, maximal activity with 0.5% CHAPS or 0.5% sodium cholate
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glutathione
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thiol-dependent enzyme, decrease of glutathione from 2 to 1 mM decreases activity by 50%
glutathione
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thiol-dependent enzyme, decrease of glutathione from 2 to 1 mM decreases activity by 50%
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2 - 11
8'-apo-beta-carotenol
2
8'-apo-beta-carotenol
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11
8'-apo-beta-carotenol
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0.000002
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from intestinal mucosa preparation
0.00002
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from intestinal mucosa preparation
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8
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UniProt
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rabbit
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rat
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A1KQY3_GIBFU
577
0
64487
TrEMBL
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G71D
mutant accumulates torulene instead of the final product of the pathway, neurosporaxanthin
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52
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30% of control activity after heating to 52°C for 3 min
52
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30% of control activity after heating to 52°C for 3 min
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expression in Escherichia coli
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Dmitrovskii, A.A.; Gessler, N.N.; Gomboeva, S.B.; Ershov, Y.V.; Bykhovsky, V.Y.
Enzymatic oxidation of beta-apo-8'-carotenol to beta-apo-14'-carotenal by an enzyme different from beta-carotene-15-15'-dioxygenase
Biochemistry (Moscow)
62
787-792
1997
Oryctolagus cuniculus, Rattus norvegicus
brenda
Prado-Cabrero, A.; Estrada, A.F.; Al-Babill, S.; Avalos, J.
Identification and biochemical characterization of a novel carotenoid oxygenase: elucidation of the cleavage step in the Fusarium carotenoid pathway
Mol. Microbiol.
64
448-460
2007
Fusarium fujikuroi (A1KQY3)
brenda
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1.13.11.67 8'-apo-beta-carotenoid 14',13'-cleaving dioxygenase
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