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EC Tree
IUBMB Comments Requires Fe2+. Also acts on 3-isopropylcatechol and 3-tert-butyl-5-methylcatechol.
The enzyme appears in viruses and cellular organisms
Synonyms
3-alkylcatechol 2,3-dioxygenase, steroid 4,5-dioxygenase, tesB,
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3-alkylcatechol 2,3-dioxygenase
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steroid 4,5-dioxygenase
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 = 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 = 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
proximal extradiol cleavage
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 = 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione:oxygen 4,5-oxidoreductase (decyclizing)
Requires Fe2+. Also acts on 3-isopropylcatechol and 3-tert-butyl-5-methylcatechol.
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
3-isopropylcatechol + O2
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3-methylcatechol
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7.4% of activity with 3-isopropylcatechol
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3-tert-butyl-5-methylcatechol + O2
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4-methylcatechol
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4.3% of activity with 3-isopropylcatechol
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
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3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
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Fe2+
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1.13 gatom per mol of enzyme, stimulation of reaction
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4-hydroxymercuribenzoate
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1 mM, 87% inhibition after 10 min
4-Isopropylcatechol
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competitive vs. organic substate, uncompetitive vs. O2
sodium iodoacetate
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1 mM, 70% inhibition after 10 min
2,2'-dipyridyl
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noncompetitive inhibition
8-hydroxyquinoline
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1 mM, 90% inhibition after 10 min, noncompetitive inhibition
o-phenanthroline
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1 mM, 68% inhibition after 10 min, noncompetitive inhibition
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brenda
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brenda
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Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
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274000
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sedimentation equilibrium
286000
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286000
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sedimentation velocity
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-20°C, 10% acetone, relatively long perids, no loss of activity
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acetone, freezing and thawing, DEAE-cellulose, calcium phosphate cellulose
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Nozaki, M.
Nonheme iron dioxygenase
Mol. Mech. Oxygen Activ. (Hayaishi, O., ed.) Academic Press, New York
135-165
1974
Rhodococcus hoagii
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brenda
Tai, H.H.; Sih, C.J.
3,4-Dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase from Nocardia restrictus. I. Isolation of the enzyme and study of its physical and chemical properties
J. Biol. Chem.
245
5062-5071
1970
Rhodococcus hoagii
brenda
Gibson, D.T.; Wang, K.C.; Sih, C.J.; Whitlock, H.
Mechanisms of steroid oxidation by microorganisms. IX. On the mechanism of ring A cleavage in the degradation of 9,10-seco steroids by microorganisms
J. Biol. Chem.
241
551-559
1966
Rhodococcus hoagii
brenda
Horinouchi, M.; Hayashi, T.; Kudo, T.
The genes encoding the hydroxylase of 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione in steroid degradation in Comamonas testosteroni TA441
J. Steroid Biochem. Mol. Biol.
92
143-154
2004
Comamonas testosteroni
brenda
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1.13.11.25 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase
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