Information on EC 1.11.1.7 - peroxidase and Organism(s) Homo sapiens

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Homo sapiens


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea


The taxonomic range for the selected organisms is: Homo sapiens

EC NUMBER
COMMENTARY hide
1.11.1.7
-
RECOMMENDED NAME
GeneOntology No.
peroxidase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
baicalein degradation (hydrogen peroxide detoxification)
-
-
betanidin degradation
-
-
justicidin B biosynthesis
-
-
luteolin triglucuronide degradation
-
-
matairesinol biosynthesis
-
-
sesamin biosynthesis
-
-
non-pathway related
-
-
Phenylpropanoid biosynthesis
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
SYSTEMATIC NAME
IUBMB Comments
phenolic donor:hydrogen-peroxide oxidoreductase
Heme proteins with histidine as proximal ligand. The iron in the resting enzyme is Fe(III). They also peroxidize non-phenolic substrates such as 3,3',5,5'-tetramethylbenzidine (TMB) and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). Certain peroxidases (e.g. lactoperoxidase, SBP) oxidize bromide, iodide and thiocyanate.
CAS REGISTRY NUMBER
COMMENTARY hide
9003-99-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
?
show the reaction diagram
-
-
-
-
?
3,3',5,5'-tetramethylbezidine + H2O2
?
show the reaction diagram
-
-
-
?
Amplex Red + H2O2
?
show the reaction diagram
-
-
-
-
?
Br- + H2O2
?
show the reaction diagram
-
-
-
-
?
Cl- + H2O2
?
show the reaction diagram
-
-
-
-
?
Cl- + H2O2
HClO + H2O
show the reaction diagram
-
-
-
-
?
Cl- + H2O2
HOCl + H2O
show the reaction diagram
-
-
-
-
?
guaiacol + H2O2
tetraguaiacol + H2O
show the reaction diagram
-
-
-
-
?
L-ascorbate + H2O2
dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
luminol + H2O2
?
show the reaction diagram
-
-
-
?
o-dianisidine + H2O2
?
show the reaction diagram
-
-
-
-
?
pyrogallol + H2O2
purpurogallin + H2O
show the reaction diagram
-
-
-
-
?
SCN- + H2O2
OSCN- + H2O
show the reaction diagram
-
-
-
-
?
taurine + Cl- + H2O2
taurine chloramine + HOCl + H2O
show the reaction diagram
-
-
-
-
?
West Pico + H2O2
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
under conditions of severe inflammation and oxidative stress, peroxidase activity of hemoglobin-haptoglobin covalent aggregates may cause macrophage dysfunction and microvascular vasoconstriction
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
under conditions of severe inflammation and oxidative stress, peroxidase activity of hemoglobin-haptoglobin covalent aggregates may cause macrophage dysfunction and microvascular vasoconstriction
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-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
69% inhibition at 5 mM
1-(5-fluoro-1H-indol-3-yl)methanamine
-
-
1-methyl-L-tryptophan
-
-
2,2'-bipyridyl
-
25% inhibition at 5 mM
2-(5-fluoro-1H-indol-3-yl)-N,N-dimethylethanamine
-
-
2-(5-fluoro-1H-indol-3-yl)-N-methylethanamine
-
-
3-(5-fluoro-1H-indol-3-yl)-N,N-dimethylpropan-1-amine
-
-
3-(5-fluoro-1H-indol-3-yl)-N-methylpropan-1-amine
-
-
3-(5-fluoro-1H-indol-3-yl)-N-propylpropan-1-amine
-
-
3-(5-fluoro-1H-indol-3-yl)propan-1-amine
-
-
4-(5-fluoro-1H-indol-3-yl)butan-1-amine
-
-
5-(5-fluoro-1H-indol-3-yl)pentan-1-amine
-
-
5-chlorotryptamine
-
-
5-fluoro-3-[(4-methylpiperazin-1-yl)methyl]-1H-indole
-
-
5-fluoro-3-[2-(4-methylpiperazin-1-yl)ethyl]-1H-indole
-
-
5-fluoro-3-[2-(pyrrolidin-1-yl)ethyl]-1H-indole
-
-
5-fluoro-3-[3-(4-methylpiperazin-1-yl)propyl]-1H-indole
-
-
5-fluoro-3-[3-(pyrrolidin-1-yl)propyl]-1H-indol
-
-
5-fluoro-3-[3-(pyrrolidin-1-yl)propyl]-1H-indole
-
-
5-fluoro-L-tryptophan
-
-
5-fluorotryptamine
-
-
6-(5-fluoro-1H-indol-3-yl)hexan-1-amine
-
-
diethyldithiocarbamate
-
87% inhibition at 1 mM
ethylene diamine tetraacetic acid
-
17% inhibition at 10 mM
Flufenamic acid
-
-
Haptoglobin
-
mixed type of inhibition, haptoglobin binds with hemoglobin and weakens hemoglobin peroxidase activity
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hydroxylamine
-
84% inhibition at 1 mM
L-tryptophan
-
-
L-tryptophan benzyl ester
-
-
N,N-diethyl-2-(5-fluoro-1H-indol-3-yl)ethanamine
-
-
N,N-diethyl-3-(5-fluoro-1H-indol-3-yl)propan-1-amine
-
-
N-ethyl-2-(5-fluoro-1H-indol-3-yl)ethanamine
-
-
N-ethyl-3-(5-fluoro-1H-indol-3-yl)propan-1-amine
-
-
N-ethyl-N-[(5-fluoro-1H-indol-3-yl)methyl]ethanamine
-
-
N-ethylmaleimide
-
18% inhibition at 5 mM
N-[2-(5-fluoro-1H-indol-3-yl)ethyl]butan-1-amine
-
-
N-[2-(5-fluoro-1H-indol-3-yl)ethyl]propan-1-amine
-
-
N-[3-(5-fluoro-1H-indol-3-yl)propyl]butan-1-amine
-
-
NaCN
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0.5 mM, 93% inhibition
Nalpha-methoxycarbonyl-L-tryptophan methyl ester
-
-
Nalpha-methyl-L-tryptophan
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-
NaN3
-
99% inhibition at 20 mM
tryptamine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid)
-
-
83.33
4-aminophenazone
-
-
1.3
Br-
-
20°C, pH 6.5
10.5
guaiacol
-
-
1.5
H2O2
isozyme VPO1
0.48
o-Dianisidine
-
-
23.8
pyrogallol
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-
0.076
SCN-
-
20°C, pH 6.5
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5
3,3',5,5'-tetramethylbezidine
isozyme VPO1
-
0.46
Br-
-
20°C, pH 6.5
13.6
SCN-
-
20°C, pH 6.5
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0009
1-(5-fluoro-1H-indol-3-yl)methanamine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00175
1-methyl-L-tryptophan
Homo sapiens
-
at 25°C in 0.2 M sodium phosphate buffer, pH 7.0
0.00009
2-(5-fluoro-1H-indol-3-yl)-N,N-dimethylethanamine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.0002
2-(5-fluoro-1H-indol-3-yl)-N-methylethanamine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00013
3-(5-fluoro-1H-indol-3-yl)-N,N-dimethylpropan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.0002
3-(5-fluoro-1H-indol-3-yl)-N-methylpropan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00017
3-(5-fluoro-1H-indol-3-yl)-N-propylpropan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00005
3-(5-fluoro-1H-indol-3-yl)propan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.000015
4-(5-fluoro-1H-indol-3-yl)butan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.000008
5-(5-fluoro-1H-indol-3-yl)pentan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.001
5-fluoro-3-[(4-methylpiperazin-1-yl)methyl]-1H-indole
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.0002
5-fluoro-3-[2-(4-methylpiperazin-1-yl)ethyl]-1H-indole
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00004
5-fluoro-3-[2-(pyrrolidin-1-yl)ethyl]-1H-indole
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00035
5-fluoro-3-[3-(4-methylpiperazin-1-yl)propyl]-1H-indole
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00032
5-fluoro-3-[3-(pyrrolidin-1-yl)propyl]-1H-indol
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00325
5-fluoro-L-tryptophan
Homo sapiens
-
at 25°C in 0.2 M sodium phosphate buffer, pH 7.0
0.0002
5-fluorotryptamine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00026
6-(5-fluoro-1H-indol-3-yl)hexan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00225
L-tryptophan
Homo sapiens
-
at 25°C in 0.2 M sodium phosphate buffer, pH 7.0
0.000175
L-tryptophan benzyl ester
Homo sapiens
-
at 25°C in 0.2 M sodium phosphate buffer, pH 7.0
0.00016
N,N-diethyl-2-(5-fluoro-1H-indol-3-yl)ethanamine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00035
N,N-diethyl-3-(5-fluoro-1H-indol-3-yl)propan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.0003
N-ethyl-2-(5-fluoro-1H-indol-3-yl)ethanamine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.0003
N-ethyl-3-(5-fluoro-1H-indol-3-yl)propan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.0002
N-ethyl-N-[(5-fluoro-1H-indol-3-yl)methyl]ethanamine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00103
N-[2-(5-fluoro-1H-indol-3-yl)ethyl]butan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.0008
N-[2-(5-fluoro-1H-indol-3-yl)ethyl]propan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.0015
N-[3-(5-fluoro-1H-indol-3-yl)propyl]butan-1-amine
Homo sapiens
-
in phosphate buffer pH 7.4, at 37°C
0.00035
Nalpha-methoxycarbonyl-L-tryptophan methyl ester
Homo sapiens
-
at 25°C in 0.2 M sodium phosphate buffer, pH 7.0
0.0052
Nalpha-methyl-L-tryptophan
Homo sapiens
-
at 25°C in 0.2 M sodium phosphate buffer, pH 7.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.05
-
with 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid)
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
oxidation of 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid)
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
thioreoxin peroxidase
Manually annotated by BRENDA team
-
thioreoxin peroxidase
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
thioreoxin peroxidase
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
-
recombinant thioredoxin peroxidase, SDS-PAGE
165000
SDS-PAGE; SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
production of rhEPO by Pichia pastoris as a glycosylated dimer
proteolytic modification
-
production of rhEPO by Pichia pastoris as a glycosylated dimer precursor of approximately 80 kDa. Proteolytic processing, similar to that in the native host, to generate two chains of approximately 50 kDa and 20 kDa
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE Sepharose column chromatography
recombinant enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expression in Pichia pastoris
-
VPO1 is expressed in HEK-293H cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
0.005-0.05 mg/ml Oenothera paradoxa extract, penta-O-galloyl-beta-D-glucose, indomethacin, catechin and gallic acid significantly inhibit myeloperoxidase release
-