Information on EC 1.1.2.2 - mannitol dehydrogenase (cytochrome)

for references in articles please use BRENDA:EC1.1.2.2
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The expected taxonomic range for this enzyme is: Acetobacteraceae

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EC NUMBER
COMMENTARY hide
1.1.2.2
-
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RECOMMENDED NAME
GeneOntology No.
mannitol dehydrogenase (cytochrome)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-mannitol + a ferricytochrome c = D-fructose + a ferrocytochrome c + 2 H+
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pentose and glucuronate interconversions
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-
Fructose and mannose metabolism
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-
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SYSTEMATIC NAME
IUBMB Comments
D-mannitol:cytochrome-c 2-oxidoreductase
The enzyme from the bacterium Gluconobacter oxydans acts on polyols with a D-lyxo configuration, such as D-mannitol and D-sorbitol, with preference towards the former.
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-78-5
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain KU-1
-
-
Manually annotated by BRENDA team
strain KU-1
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyclic D-erythro polyols + ferricytochrome c
acyclic D-erythro ketoses + ferrocytochrome c
show the reaction diagram
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
D-sorbitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome c
methylene blue
-
suitable as electron acceptor
phenazine methosulfate
-
suitable as electron acceptor
additional information
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
restores activity after inactivation with EDTA and storage
Mg2+
-
restores activity after inactivation with EDTA and storage
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Hg2+
-
strong inhibitor
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17
D-arabitol
-
-
0.098 - 34
D-mannitol
20
D-sorbitol
-
-
44
ribitol
-
-
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18
p-quinone
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE
51000
-
alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE
69000
-
alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE
140000
-
gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 7
-
stable for 30 min at 30°C
389622
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
stable up to 50°C at pH 6
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, stable for several weeks, lost activity can be restored by adding Ca2+ or Mg2+
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.2.2)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)