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EC Tree
The expected taxonomic range for this enzyme is: Escherichia coli
Synonyms
dtdp-6-deoxy-d-xylo-hex-4-ulopyranose reductase,
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dTDP-6-deoxy-D-xylo-hex-4-ulopyranose reductase
dTDP-6-deoxy-D-xylo-hex-4-ulopyranose reductase
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dTDP-6-deoxy-D-xylo-hex-4-ulopyranose reductase
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Fcf1
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dTDP-alpha-D-fucopyranose + NAD(P)+ = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD(P)H + H+
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dTDP-alpha-D-fucopyranose:NAD(P)+ oxidoreductase
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dTDP-alpha-D-fucopyranose + NADH + H+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+
dTDP-alpha-D-fucopyranose + NADP+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
dTDP-alpha-D-fucopyranose + NADPH + H+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+
dTDP-alpha-D-fucopyranose + NADH + H+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+
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NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
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dTDP-alpha-D-fucopyranose + NADH + H+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+
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NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
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dTDP-alpha-D-fucopyranose + NADP+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
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the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
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dTDP-alpha-D-fucopyranose + NADP+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
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the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
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dTDP-alpha-D-fucopyranose + NADPH + H+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+
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NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
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dTDP-alpha-D-fucopyranose + NADPH + H+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+
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NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
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dTDP-alpha-D-fucopyranose + NADP+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
dTDP-alpha-D-fucopyranose + NADP+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
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the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
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dTDP-alpha-D-fucopyranose + NADP+
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
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the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
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NADH
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NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
NADPH
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NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
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additional information
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Fcf1 is a divalent cation-independent enzyme
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Co2+
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5 mM, 11% inhibition
Cu2+
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5 mM, 88% inhibition
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0.38
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
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37°C, pH 7.4
0.89
NADPH
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37°C, pH 7.4
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2.78
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
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37°C, pH 7.4
26.67
NADH
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37°C, pH 7.4
6.76
NADPH
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37°C, pH 7.4
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7.4
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
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37°C, pH 7.4
31.75
NADH
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37°C, pH 7.4
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4 - 50
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4°C: 47% of maximal activity, 50°C: 36% of maximal activity
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brenda
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brenda
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malfunction
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mutant strains H1862 lacking fcf1. The mutant strain produces semirough lipopolysaccharide with only one O unit attached to the core-lipid A moiety while the wild type strain produces normal lipopolysaccharide. The result indicates that fcf1 is required for the synthesis of the O-antigen in Escherichia coli O52
malfunction
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mutant strains H1862 lacking fcf1. The mutant strain produces semirough lipopolysaccharide with only one O unit attached to the core-lipid A moiety while the wild type strain produces normal lipopolysaccharide. The result indicates that fcf1 is required for the synthesis of the O-antigen in Escherichia coli O52
physiological function
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the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O-antigen
physiological function
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the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O-antigen
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40700
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x * 40700, His6-tagged fusion protein, SDS-PAGE
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x * 40700, His6-tagged fusion protein, SDS-PAGE
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x * 40700, His6-tagged fusion protein, SDS-PAGE
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-20°C, in 50% glycerol, stable for at least 5 months
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His6-tagged fusion protein
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overexpression in Escherichia coli BL21
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Wang, Q.; Ding, P.; Perepelov, A.V.; Xu, Y.; Wang, Y.; Knirel, Y.A.; Wang, L.; Feng, L.
Characterization of the dTDP-D-fucofuranose biosynthetic pathway in Escherichia coli O52
Mol. Microbiol.
70
1358-1367
2008
Escherichia coli, Escherichia coli O52
brenda
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1.1.1.B28 dTDP-4-dehydro-6-deoxyglucose reductase [NAD(P)H]
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