Information on EC 1.1.1.97 - 3-hydroxybenzyl-alcohol dehydrogenase

for references in articles please use BRENDA:EC1.1.1.97
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

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EC NUMBER
COMMENTARY hide
1.1.1.97
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RECOMMENDED NAME
GeneOntology No.
3-hydroxybenzyl-alcohol dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-hydroxybenzyl alcohol + NADP+ = 3-hydroxybenzaldehyde + NADPH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
patulin biosynthesis
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Toluene degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
3-hydroxybenzyl-alcohol:NADP+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
9075-73-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxybenzyl alcohol + NADP+
3-hydroxybenzaldehyde + NADPH
show the reaction diagram
3-methoxybenzyl alcohol + NADP+
3-methoxybenzaldehyde + NADPH
show the reaction diagram
4-hydroxybenzyl alcohol + NADP+
4-hydroxybenzaldehyde + NADPH
show the reaction diagram
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
ethanol + NADP+
acetaldehyde + NADPH + H+
show the reaction diagram
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29% activity compared to 3-hydroxybenzyl alcohol
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r
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-hydroxybenzyl alcohol + NADP+
3-hydroxybenzaldehyde + NADPH
show the reaction diagram
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diethyldicarbonate
iodoacetic acid
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
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1.0 mM and 10 mM, 56% increase in activity, enzyme activity in cell extracts
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
3-Hydroxybenzyl alcohol
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.57
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enzyme activity in crude extracts from cells grown on 3,5-xylenol
0.65
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enzyme activity in crude extracts from cells grown on 3-hydroxy-5-methylbenzoate
0.7
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enzyme activity in crude extracts from cells grown on gentisate
0.78
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enzyme activity in crude extracts from cells grown on m-hxdroxybenzoate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
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4 * 36000, SDS-PAGE
120000
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sedimentation equilibrium
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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4 * 36000, SDS-PAGE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
14 min half-life of crude enzyme at 30°C in 100 mM Tris-HCl, pH 7.6, half-life is increased to 77 and 80 min by 2 mM MnCl2 and FeCl3 respectively, 3 mM NADPH increase half-life to 146 min
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Bio-Gel A, 2',5'-ADP-agarose, DEAE-Sephacell, 2',5'-ADP-agarose
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streptomycin sulfate, ammonium sulfate, Sephadex G-200, 6.55fold purification
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.97)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)