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IUBMB Comments Substrates contain an aromatic ring with a pyruvate side chain. The most active substrates are halogenated derivatives. Compounds with hydroxy or amino groups in the 3 or 5 position are inactive.
Synonyms
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2-oxo acid reductase
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aromatic alpha-keto acid reductase
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3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+ = 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH + H+
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3-(3,5-diiodo-4-hydroxyphenyl)lactate:NAD+ oxidoreductase
Substrates contain an aromatic ring with a pyruvate side chain. The most active substrates are halogenated derivatives. Compounds with hydroxy or amino groups in the 3 or 5 position are inactive.
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3,5-dinitro-4-hydroxyphenylpyruvate + NADH
3,5-dinitro-4-hydroxyphenyllactate + NAD+
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Substrates: -
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3-(3,5-dibromo-4-hydroxyphenyl)pyruvate + NADH
3-(3,5-dibromo-4-hydroxyphenyl)lactate + NAD+
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Substrates: -
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3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
3-bromo-4-hydroxyphenylpyruvate + NADH
3-bromo-4-hydroxyphenyllactate + NAD+
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Substrates: -
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3-fluorophenylpyruvate + NADH
3-fluorophenyllactate + NAD+
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Substrates: -
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4-hydroxyphenylpyruvate + NADH + H+
4-hydroxyphenyllactate + NAD+
indolepyruvate + NADH
indolelactate + NAD+
oxaloacetate + NADH
malate + NAD+
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Substrates: order of activity, oxaloacetate, 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate, indolepyruvate, phenylpyruvate
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phenylpyruvate + NADH
phenyllactate + NAD+
3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
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Substrates: formation of phenyllactic acids is strongly favoured
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3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
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Substrates: -
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3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
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Substrates: -
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4-hydroxyphenylpyruvate + NADH + H+
4-hydroxyphenyllactate + NAD+
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Substrates: -
r
4-hydroxyphenylpyruvate + NADH + H+
4-hydroxyphenyllactate + NAD+
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Substrates: -
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indolepyruvate + NADH
indolelactate + NAD+
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Substrates: -
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indolepyruvate + NADH
indolelactate + NAD+
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Substrates: -
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phenylpyruvate + NADH
phenyllactate + NAD+
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Substrates: -
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phenylpyruvate + NADH
phenyllactate + NAD+
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Substrates: -
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phenylpyruvate + NADH
phenyllactate + NAD+
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Substrates: -
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NADPH
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15% activity compared to NADH
NADH
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2,5-dihydroxybenzoic acid
2,5-dihydroxyphenyllactic acid
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1 mM, 20% inhibition
3,5-Diiodo-4-hydroxyphenyllactic acid
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1 mM, 18% inhibition
3,5-diiodotyrosine
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1 mM, 15% inhibition
2,5-dihydroxybenzoic acid
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1 mM, 44% inhibition
2,5-dihydroxybenzoic acid
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0.5 mM, 60% inhibition
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0.04 - 0.043
3,5-diiodo-4-hydroxyphenylpyruvate
1.9
4-hydroxyphenylpyruvate
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0.04
3,5-diiodo-4-hydroxyphenylpyruvate
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0.043
3,5-diiodo-4-hydroxyphenylpyruvate
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0.014
NADH
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1.6
phenylpyruvate
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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MDHC_HUMAN
334
0
36426
Swiss-Prot
Secretory Pathway (Reliability: 5 )
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40000
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2 * 40000, SDS-PAGE
75000
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sucrose-density gradient centrifugation
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dimer
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2 * 40000, SDS-PAGE
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62
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not completely inactivated after 2 min, completely inactivated above 72°C
72
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complete inactivation in 2 min
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-20°C, 50 mM potassium phosphate, pH 7.5, 2 months, no loss of activity
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4°C, 50 mM potassium phosphate, pH 7.5, 2 weeks, no loss of activity
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5°C, 160 mM phosphate, pH 6.5, 1 month, 20% activity lost
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ammonium sulfate, calcium phosphate gel
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DEAE-cellulose, hydroxyapatite, isoelectric focusing, Sephadex G-150
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Donald, L.J.
A description of human aromatic alpha-keto acid reductase
Ann. Hum. Genet.
46
299-306
1982
Canis lupus familiaris, Homo sapiens
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Takada, Y.; Noguchi, T.; Kido, R.
Purification, characterization and identification of aromatic 2-oxo acid reductase
Life Sci.
20
609-616
1977
Canis lupus familiaris
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Zannoni, V.G.; Weber, W.W.
Isolation and properties of aromatic alpha-keto acid reductase
J. Biol. Chem.
241
1340-1344
1966
Bos taurus, Canis lupus familiaris, Cavia porcellus
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