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IUBMB Comments The enzyme, characterized from the plant Nepeta mussinii , binds an NAD+ cofactor. It also catalyses the activity of EC 5.5.1.34 , (+)-cis ,trans -nepetalactol synthase.
The expected taxonomic range for this enzyme is: Nepeta racemosa
Synonyms neps1, more
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nepetalactol-related short-chain dehydrogenase/reductase 1
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nepetalactol-related short-chain-dehydrogenase/reductase
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NEPS1
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(+)-cis,cis-nepetalactol + NAD+ = (+)-cis,cis-nepetalactone + NADH + H+
(1)
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(+)-cis,trans-nepetalactol + NAD+ = (+)-cis,trans-nepetalactone + NADH + H+
(2)
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MetaCyc
nepetalactone biosynthesis
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nepetalactol:NAD+ 1-oxidoreductase
The enzyme, characterized from the plant Nepeta mussinii, binds an NAD+ cofactor. It also catalyses the activity of EC 5.5.1.34, (+)-cis,trans-nepetalactol synthase.
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(+)-cis,cis-nepetalactol + NAD+
(+)-cis,cis-nepetalactone + NADH + H+
(+)-cis,trans-nepetalactol + NAD+
(+)-cis,trans-nepetalactone + NADH + H+
(+)-cis,cis-nepetalactol + NAD+
(+)-cis,cis-nepetalactone + NADH + H+
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Substrates: - Products: -
r
(+)-cis,cis-nepetalactol + NAD+
(+)-cis,cis-nepetalactone + NADH + H+
Substrates: - Products: -
r
(+)-cis,trans-nepetalactol + NAD+
(+)-cis,trans-nepetalactone + NADH + H+
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Substrates: - Products: -
r
(+)-cis,trans-nepetalactol + NAD+
(+)-cis,trans-nepetalactone + NADH + H+
Substrates: - Products: -
r
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(+)-cis,cis-nepetalactol + NAD+
(+)-cis,cis-nepetalactone + NADH + H+
(+)-cis,trans-nepetalactol + NAD+
(+)-cis,trans-nepetalactone + NADH + H+
(+)-cis,cis-nepetalactol + NAD+
(+)-cis,cis-nepetalactone + NADH + H+
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Substrates: - Products: -
r
(+)-cis,cis-nepetalactol + NAD+
(+)-cis,cis-nepetalactone + NADH + H+
Substrates: - Products: -
r
(+)-cis,trans-nepetalactol + NAD+
(+)-cis,trans-nepetalactone + NADH + H+
-
Substrates: - Products: -
r
(+)-cis,trans-nepetalactol + NAD+
(+)-cis,trans-nepetalactone + NADH + H+
Substrates: - Products: -
r
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NAD+
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Na+
0.1 M NaCl used in assay conditions
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brenda
catmint
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brenda
catmint
UniProt
brenda
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brenda
Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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NEPS1_NEPRA
271
0
28683
Swiss-Prot
other Location (Reliability: 3 )
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Ni-NTA agarose column chromatography, HisTrap column chromatography and Superdex 200 gel filtration
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expressed in Escherichia coli BL21 cells
expressed in Saccharomyces cerevisiae
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Lichman, B.; O'Connor, S.; Kries, H.
Biocatalytic strategies towards [4+2] cycloadditions
Chemistry
25
6864-6877
2019
Nepeta racemosa
brenda
Billingsley, J.M.; Anguiano, J.L.; Tang, Y.
Production of semi-biosynthetic nepetalactone in yeast
J. Ind. Microbiol. Biotechnol.
46
1365-1370
2019
Nepeta racemosa
brenda
Lichman, B.R.; Kamileen, M.O.; Titchiner, G.R.; Saalbach, G.; Stevenson, C.E.M.; Lawson, D.M.; O'Connor, S.E.
Uncoupled activation and cyclization in catmint reductive terpenoid biosynthesis
Nat. Chem. Biol.
15
71-79
2019
Nepeta racemosa (A0A3Q8GL18)
brenda
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