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EC Tree
IUBMB Comments The enzyme, found in plants, catalyses multiple reactions during plant sterol biosynthesis. Unlike the fungal/animal enzyme EC 1.1.1.170, 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating), the plant enzyme is specific for NAD+.
The enzyme appears in viruses and cellular organisms
Synonyms
3betahsd/d, 4alpha-carboxysterol-c3-dehydrogenase/c4-decarboxylase, 4alpha-cd, 3betahsd/d2, 3beta-hydroxysteroid-dehydrogenase/c4-decarboxylase,
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3beta-hydroxysteroid dehydrogenase/C-4 decarboxylase
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3beta-hydroxysteroid dehydrogenases/C-4 decarboxylase
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ambiguous
3beta-hydroxysteroid-dehydrogenase/C4-decarboxylase
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4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase
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a 3beta-hydroxysteroid-4alpha-carboxylate + NAD+ = a 3-oxosteroid + CO2 + NADH
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3beta-hydroxysteroid-4alpha-carboxylate:NAD+ 3-oxidoreductase (decarboxylating)
The enzyme, found in plants, catalyses multiple reactions during plant sterol biosynthesis. Unlike the fungal/animal enzyme EC 1.1.1.170, 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating), the plant enzyme is specific for NAD+.
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3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
? + CO2 + NADH + H+
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-
?
3beta-hydroxy,4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-sterol + NAD+
4alpha,14-dimethyl-5alpha,9beta-9,19-cycloergost-24(28)-en-3beta-ol + CO2 + NADH + H+
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-
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?
4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-cholest-24-en-3beta-ol + NAD+
cholest-7-en-3-one + CO2 + NADH + H+
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-
-
?
4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cycloergost-24(241)-en-3beta-ol + NAD+
4alpha,14alpha-dimethyl-9beta,19-cyclo-ergost-24(241)-en-3-one + CO2 + NADH + H+
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?
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol + NAD+
4alpha-methyl-cholest-8,24-dien-3-one + CO2 + NADH + H+
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?
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol + NAD+
? + CO2 + NADH + H+
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?
4alpha-carboxy-5alpha-stigmastane-3beta-ol + NAD+
stigmastane-3-one + CO2 + NADH + H+
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?
4alpha-carboxy-cholest-7-en-3alpha-ol + NAD+
cholest-7-en-3-one + CO2 + NADH + H+
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?
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
? + CO2 + NADH + H+
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?
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
cholest-7-en-3-one + CO2 + NADH + H+
4alpha-carboxy-stigmasta-7,24(241)-dien-3beta-ol + NAD+
stigmasta-7,24(241)-dien-3-one + CO2 + NADH + H+
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-
?
4alpha-methyl-cholest-8,24-dien-3-one + NAD+
? + CO2 + NADH + H+
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?
4alpha-[242-3H]carboxy-5alpha-stigmasta-7,24(241)-dien-3beta-ol + NAD+
? + CO2 + NADH + H+
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?
4beta,14alpha-[241-3H]dimethyl-4alpha-carboxy-5alpha-ergosta-9beta,19-cyclo-24(241)-en-3beta-ol + NAD+
? + CO2 + NADH + H+
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?
a 3beta-hydroxysteroid-4alpa-carboxylate + NAD+
a 3-oxosteroid + CO2 + NADH + H+
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?
a 3beta-hydroxysteroid-4alpha-carboxylate + NAD+
a 3-oxosteroid + CO2 + NADH + H+
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?
additional information
?
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the decarboxylation reaction is independent of molecular oxygen
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?
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
cholest-7-en-3-one + CO2 + NADH + H+
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?
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
cholest-7-en-3-one + CO2 + NADH + H+
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?
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a 3beta-hydroxysteroid-4alpa-carboxylate + NAD+
a 3-oxosteroid + CO2 + NADH + H+
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?
a 3beta-hydroxysteroid-4alpha-carboxylate + NAD+
a 3-oxosteroid + CO2 + NADH + H+
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?
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NAD+
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NAD+
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strong preference for NAD+
NAD+
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NAD+ is more efficient than NADP+
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0.081 - 0.112
3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol
0.2 - 0.77
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
0.383 - 0.655
4alpha-carboxy-cholest-7-en-3alpha-ol
0.087 - 0.51
4alpha-carboxy-cholest-7-en-3beta-ol
0.081
3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol
isoform 3betaHSD/D2, at 30°C, pH not specified in the publication
0.112
3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol
isoform 3betaHSD/D1, at 30°C, pH not specified in the publication
0.2
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
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mutant enzyme S133A, pH and temperature not specified in the publication
0.204
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
isoform 3betaHSD/D2, at 30°C, pH not specified in the publication
0.26
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
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wild type enzyme, pH and temperature not specified in the publication
0.274
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
isoform 3betaHSD/D1, at 30°C, pH not specified in the publication
0.77
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol
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mutant enzyme S133T, pH and temperature not specified in the publication
0.383
4alpha-carboxy-cholest-7-en-3alpha-ol
isoform 3betaHSD/D1, at 30°C, pH not specified in the publication
0.655
4alpha-carboxy-cholest-7-en-3alpha-ol
isoform 3betaHSD/D2, at 30°C, pH not specified in the publication
0.087
4alpha-carboxy-cholest-7-en-3beta-ol
isoform 3betaHSD/D2, at 30°C, pH not specified in the publication
0.11
4alpha-carboxy-cholest-7-en-3beta-ol
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mutant enzyme S133A, pH and temperature not specified in the publication
0.13
4alpha-carboxy-cholest-7-en-3beta-ol
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wild type enzyme, pH and temperature not specified in the publication
0.134
4alpha-carboxy-cholest-7-en-3beta-ol
isoform 3betaHSD/D1, at 30°C, pH not specified in the publication
0.51
4alpha-carboxy-cholest-7-en-3beta-ol
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mutant enzyme S133T, pH and temperature not specified in the publication
0.0031
NAD+
isoform 3betaHSD/D2, at 30°C, pH not specified in the publication
0.008
NAD+
isoform 3betaHSD/D1, at 30°C, pH not specified in the publication
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brenda
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UniProt
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HSDD2_ARATH
564
3
61704
Swiss-Prot
other Location (Reliability: 5 )
HSDD1_ARATH
439
2
48125
Swiss-Prot
other Location (Reliability: 4 )
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?
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x * 42000, SDS-PAGE
?
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x * 45000, enzyme in sodium cholate, SDS-PAGE
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R318I
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the mutant shows slightly reduced activity compared to the wild type enzyme
S131A
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the mutant shows reduced activity compared to the wild type enzyme
S133A
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the mutant shows reduced activity compared to the wild type enzyme
S133T
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the mutant shows reduced activity compared to the wild type enzyme
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treatment of microsomes with sodium cholate at a final concentration of 1% (10 mg/ml) leads to 58% solubilization of the enzyme activity, while Brij W-1 at a final concentration of 1% (10 mg/ml) yields 115% recovery of the initial enzyme activity
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DEAE-Trisacryl column chromatography, Cibacron blue 3GA agarose dye chromatography, and Sephacryl S-100 gel filtration
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expressed in Saccharomyces cerevisiae strain erg26
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expressed in Saccharomyces cerevisiae strain erg26 SDG200
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Rondet, S.; Taton, M.; Rahier, A.
Identification, characterization, and partial purification of 4apha-carboxysterol-C3-dehydrogenase/C4-decarboxylase from Zea mays
Arch. Biochem. Biophys.
366
249-260
1999
Zea mays
brenda
Rahier, A.; Darnet, S.; Bouvier, F.; Camara, B.; Bard, M.
Molecular and enzymatic characterizations of novel bifunctional 3beta-hydroxysteroid dehydrogenases/C-4 decarboxylases from Arabidopsis thaliana
J. Biol. Chem.
281
27264-27277
2006
Arabidopsis thaliana (Q67ZE1), Arabidopsis thaliana (Q9FX01)
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brenda
Rahier, A.; Bergdoll, M.; Genot, G.; Bouvier, F.; Camara, B.
Homology modeling and site-directed mutagenesis reveal catalytic key amino acids of 3beta-hydroxysteroid-dehydrogenase/C4-decarboxylase from Arabidopsis[C][W]
Plant Physiol.
149
1872-1886
2009
Arabidopsis thaliana
brenda
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1.1.1.418 plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating)
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