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EC Tree
IUBMB Comments Involved in the catabolism of ascorbate (vitamin C) to tartrate. No activity is observed with NADP+ (cf. EC 1.1.1.264, L-idonate 5-dehydrogenase).
The enzyme appears in viruses and cellular organisms
Synonyms
L-IdnDH, L-idonate dehydrogenase, LidnDH,
more
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L-IdnDH
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L-idonate dehydrogenase
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L-idonate dehydrogenase
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L-idonate dehydrogenase
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L-idonate + NAD+ = 5-dehydro-D-gluconate + NADH + H+
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L-idonate:NAD+ oxidoreductase
Involved in the catabolism of ascorbate (vitamin C) to tartrate. No activity is observed with NADP+ (cf. EC 1.1.1.264, L-idonate 5-dehydrogenase).
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L-idonate + NAD+
5-dehydro-D-gluconate + NADH + H+
sorbitol + NAD+
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when sorbitol is tested as a substrate, NAD+ reduction occurs but at a very low rate
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r
additional information
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no activity with NADPH and NADP+
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L-idonate + NAD+
5-dehydro-D-gluconate + NADH + H+
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r
L-idonate + NAD+
5-dehydro-D-gluconate + NADH + H+
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L-idonate + NAD+
5-dehydro-D-gluconate + NADH + H+
sorbitol + NAD+
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when sorbitol is tested as a substrate, NAD+ reduction occurs but at a very low rate
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r
additional information
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no activity with NADPH and NADP+
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L-idonate + NAD+
5-dehydro-D-gluconate + NADH + H+
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L-idonate + NAD+
5-dehydro-D-gluconate + NADH + H+
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NAD+
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SwissProt
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SwissProt
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SwissProt
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cv. Cabernet Sauvignon, clone338
SwissProt
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grape berries from regions Changli (CL) and Gaotai (GT)in eastern and western China,respectively. Some genes required for carbohydrate metabolism, such as hexose transporter, L-idonate dehydrogenase, and phosphoenolpyruvate carboxylase, are significantly upregulated in the CL berries in relation to the GT berries, which positively correlated with the sugar and organic acid accumulations
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high expression level in full bloom, almost no transcript of the enzyme in mature berries
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high expression level in full bloom, almost no transcript of the enzyme in mature berries
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high expression level in full bloom, almost no transcript of the enzyme in mature berries
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low in 21d berries
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low in 21d berries
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low in 21d berries
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low in 21d berries
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low in 21d berries
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low in 21d berries
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mainly in 21d and 60d berries
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mainly in 21d and 60d berries
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mainly in 21d and 60d berries
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low in 21d berries
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low in 21d berries
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low in 21d berries
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mainly in 60d berries
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mainly in 60d berries
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mainly in 60d berries
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physiological function
enzyme LidnDH catalyzes the conversion of L-idonate to 5-keto D-gluconic acid in plant and is proposed to be a rate-limiting step in biosynthesis of tartaric acid from ascorbic acid
additional information
cultivar Cabernet Sauvignon transcriptome analysis dependent on regional parameters of six different samples, overview. Two unigenes encoding L-idonate dehydrogenase (LidnDH) are detected from the assembled transcriptome. One is expressed higher in Changli region berries than in Gaotai region berries at E-L 31 stage,while the other is expressed higher at E-L 38 stage in Gaotai region berries
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IDND_VITVI
366
0
39466
Swiss-Prot
other Location (Reliability: 1 )
A0A5B7AVX9_DAVIN
376
0
40565
TrEMBL
other Location (Reliability: 2 )
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40000
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x * 40000, SDS-PAGE
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expressed in Escherichia coli BL21(DE3) pLysS cells
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gene LidnDH, DNA library construction from a single pool of six samples, DNA sequence determination and comparisons, quantitative real-time PCR enzyme expression analysis in different regional samples of cv. Cabernet Sauvignon berries
IdnDh gene, quantitative expression analysis on transcriptional and translational levels in fruits of different stages
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DeBolt, S.; Cook, D.R.; Ford, C.M.
L-tartaric acid synthesis from vitamin C in higher plants
Proc. Natl. Acad. Sci. USA
103
5608-5613
2006
Vitis vinifera
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Wen, Y.Q.; Li, J.M.; Zhang, Z.Z.; Zhang, Y.F.; Pan, Q.H.
Antibody preparation, gene expression and subcellular localization of L-idonate dehydrogenase in grape berry
Biosci. Biotechnol. Biochem.
74
2413-2417
2010
Vitis vinifera (Q1PSI9), Vitis vinifera Syrah (Q1PSI9), Vitis vinifera Cabernet Sauvignon (Q1PSI9)
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Sun, R.; He, F.; Lan, Y.; Xing, R.; Liu, R.; Pan, Q.; Wang, J.; Duan, C.
Transcriptome comparison of Cabernet Sauvignon grape berries from two regions with distinct climate
J. Plant Physiol.
178
43-54
2015
Vitis vinifera (Q1PSI9)
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