We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Isolated from the mold Aspergillus parasiticus. Involved in a metabolic grid that leads to aflatoxin biosynthesis.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
vha reductase, vha reductase i, vha reductase ii,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
VHA reductase I
-
-
-
VHA reductase II
-
-
-
vrdA
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
versicolorone + NADP+ = 1'-hydroxyversicolorone + NADPH + H+
(1)
-
-
versiconol + NADP+ = versiconal + NADPH + H+
(3)
-
-
versiconol acetate + NADP+ = versiconal hemiacetal acetate + NADPH + H+
(2)
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
versiconol-acetate:NADP+ oxidoreductase
Isolated from the mold Aspergillus parasiticus. Involved in a metabolic grid that leads to aflatoxin biosynthesis.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1'-hydroxyversicolorone + NADPH + H+
versicolorone + NADP+
versiconal + NADPH + H+
versiconol + NADP+
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
1'-hydroxyversicolorone + NADPH + H+
versicolorone + NADP+
-
-
-
r
1'-hydroxyversicolorone + NADPH + H+
versicolorone + NADP+
-
-
r
1'-hydroxyversicolorone + NADPH + H+
versicolorone + NADP+
-
-
-
r
versiconal + NADPH + H+
versiconol + NADP+
-
-
-
r
versiconal + NADPH + H+
versiconol + NADP+
-
-
r
versiconal + NADPH + H+
versiconol + NADP+
-
-
-
r
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
-
-
-
r
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
-
-
r
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
-
both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced
-
r
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
-
-
-
r
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
-
both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced
-
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
versiconal + NADPH + H+
versiconol + NADP+
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
versiconal + NADPH + H+
versiconol + NADP+
-
-
-
-
r
versiconal + NADPH + H+
versiconol + NADP+
-
-
-
-
r
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
-
-
-
-
r
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
-
both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced
-
-
r
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
-
-
-
-
r
versiconal hemiacetal acetate + NADPH + H+
versiconol acetate + NADP+
-
both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced
-
-
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NADPH
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0254 - 0.0354
versiconal hemiacetal acetate
0.0254
versiconal hemiacetal acetate
-
isoform VHA reductase II, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C
0.0354
versiconal hemiacetal acetate
-
isoform VHA reductase I, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00362
-
crude extract, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C
0.027
-
isoform VHA reductase II, after 7.5fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C
0.052
-
isoform VHA reductase I, after 14.4fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8
-
isoform VHA reductase I
9
-
isoform VHA reductase II
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.5 - 9
-
no activity is detected below pH 4.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6
-
isoform VHA reductase II, isoelectric focusing
6.6
-
isoform VHA reductase I, isoelectric focusing
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
metabolism
-
the enzyme is specifically involved in aflatoxin biosynthesis
metabolism
the vrdA is not an aflatoxin biosynthesis gene, although it actually participates in aflatoxin biosynthesis in cells because of its group substrate specificity to intermediates in aflatoxin biosynthesis
metabolism
-
the enzyme is specifically involved in aflatoxin biosynthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
VRDA_ASPPA
343
0
38698
Swiss-Prot
other Location (Reliability: 2 )
A0A0H4NVF3_9BACI
300
0
34516
TrEMBL
-
A0A0C7NTE3_DEFTU
301
0
34641
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
38566
x * 38566, calculated from amino acid sequence
39000
-
10 * 39000, isoform VHA reductase I, SDS-PAGE
40000
-
10 * 40000, isoform VHA reductase II, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 38566, calculated from amino acid sequence
homodecamer
-
10 * 39000, isoform VHA reductase I, SDS-PAGE
homodecamer
-
10 * 40000, isoform VHA reductase II, SDS-PAGE
homodecamer
-
10 * 39000, isoform VHA reductase I, SDS-PAGE
homodecamer
-
10 * 40000, isoform VHA reductase II, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ammonium sulfate fractionation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, Sephacryl S-300 gel filtration, hydroxylapatite column chromatography, and Matrex gel Green A chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Matsushima, K.; Ando, Y.; Hamasaki, T.; Yabe, K.
Purification and characterization of two versiconal hemiacetal acetate reductases involved in aflatoxin biosynthesis
Appl. Environ. Microbiol.
60
2561-2567
1994
Aspergillus parasiticus, Aspergillus parasiticus NUIH-26
brenda
Yabe, K.; Chihaya, N.; Hamamatsu, S.; Sakuno, E.; Hamasaki, T.; Nakajima, H.; Bennett, J.W.
Enzymatic conversion of averufin to hydroxyversicolorone and elucidation of a novel metabolic grid involved in aflatoxin biosynthesis
Appl. Environ. Microbiol.
69
66-73
2003
Aspergillus parasiticus, Aspergillus parasiticus NIAH-26
brenda
Shima, Y.; Shiina, M.; Shinozawa, T.; Ito, Y.; Nakajima, H.; Adachi, Y.; Yabe, K.
Participation in aflatoxin biosynthesis by a reductase enzyme encoded by vrdA gene outside the aflatoxin gene cluster
Fungal Genet. Biol.
46
221-231
2009
Aspergillus parasiticus (B9WYE6)
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
1.1.1.353 versiconal hemiacetal acetate reductase
more
top
Information
