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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
1.1.1.280
branched-chain
transaminase
glioblastoma
neoplasia
non-neoplastic
invasiveness
hypermethylation
synthesis
Synonyms 2-methyl-3-oxobutanoate reductase, 3-oxo ester (S)-reductase, EC 1.2.1.56, reductase, 3-oxo ester (S)-, more
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2-methyl-3-oxobutanoate reductase
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3-oxo ester (S)-reductase
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EC 1.2.1.56
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formerly
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reductase, 3-oxo ester (S)-
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ethyl (S)-3-hydroxyhexanoate + NADP+ = ethyl 3-oxohexanoate + NADPH + H+
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ethyl-(S)-3-hydroxyhexanoate:NADP+ 3-oxidoreductase
Also acts on 4-oxo- and 5-oxo-fatty acids and their esters. cf. EC 1.1.1.279 (R)-3-hydroxyacid-ester dehydrogenase.
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2-methyl-3-oxobutanoate + NADPH + H+
(2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
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Substrates: - Products: -
r
4-oxopentanoate + NADPH
(S)-4-hydroxypentanoate + NADP+
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Substrates: - Products: -
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5-oxohexanoate + NADPH
(S)-5-hydroxyhexanoate + NADP+
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Substrates: - Products: -
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benzyl 2-methyl-3-oxobutanoate + NADPH
benzyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
ethyl 2-methyl-3-oxobutanoate + NADPH
ethyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
ethyl 2-oxocyclohexanecarboxylate + NADPH
ethyl (1R,2S)-2-hydroxycyclohexanecarboxylate + NADP+
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Substrates: - Products: -
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ethyl 2-oxocyclopentanecarboxylate + NADPH
ethyl (1R,2S)-2-hydroxycyclopentanecarboxylate + NADP+
ethyl 3-oxobutyrate + NADPH
ethyl (S)-3-hydroxybutyrate + NADP+
ethyl 3-oxohexanoate + NADPH
ethyl (S)-3-hydroxyhexanoate + NADP+
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Substrates: - Products: -
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additional information
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benzyl 2-methyl-3-oxobutanoate + NADPH
benzyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
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Substrates: - Products: -
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benzyl 2-methyl-3-oxobutanoate + NADPH
benzyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
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Substrates: - Products: -
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ethyl 2-methyl-3-oxobutanoate + NADPH
ethyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
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Substrates: - Products: -
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ethyl 2-methyl-3-oxobutanoate + NADPH
ethyl (2R,3S)-3-hydroxy-2-methylbutanoate + NADP+
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Substrates: - Products: -
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ethyl 2-oxocyclopentanecarboxylate + NADPH
ethyl (1R,2S)-2-hydroxycyclopentanecarboxylate + NADP+
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Substrates: - Products: -
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ethyl 2-oxocyclopentanecarboxylate + NADPH
ethyl (1R,2S)-2-hydroxycyclopentanecarboxylate + NADP+
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Substrates: - Products: -
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ethyl 3-oxobutyrate + NADPH
ethyl (S)-3-hydroxybutyrate + NADP+
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Substrates: - Products: -
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ethyl 3-oxobutyrate + NADPH
ethyl (S)-3-hydroxybutyrate + NADP+
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Substrates: - Products: -
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ethyl 3-oxobutyrate + NADPH
ethyl (S)-3-hydroxybutyrate + NADP+
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Substrates: - Products: -
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additional information
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Substrates: ethyl (2R,3S)-3-hydroxy-2-methylbutanoate is a useful starting material for the synthesis of ferroelectric liquid crystal compounds and various other biologically active substances Products: -
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additional information
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Substrates: ethyl (2R,3S)-3-hydroxy-2-methylbutanoate is a useful starting material for the synthesis of ferroelectric liquid crystal compounds and various other biologically active substances Products: -
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NADPH
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specific for
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8-hydroxyquinoline 5-sulfonic acid
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Cu2+
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1 mM completely inactivates the enzyme
Fe2+
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1 mM completely inactivates the enzyme
Mn2+
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1 mM completely inactivates the enzyme
Zn2+
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1 mM completely inactivates the enzyme
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5.6
2-methyl-3-oxobutanoate
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12.5
benzyl 2-methyl-3-oxobutanoate
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0.9
ethyl 3-oxobutyrate
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5.3
ethyl 3-oxohexanoate
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IFO3319
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brenda
IFO3319
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brenda
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brenda
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brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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24000
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2 * 24000, SDS-PAGE
31000
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1 * 31000, SDS-PAGE
48000 - 50000
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gel filtration
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dimer
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2 * 24000, SDS-PAGE
monomer
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1 * 31000, SDS-PAGE
monomer
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1 * 31000, SDS-PAGE
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45
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stable up to 45øC, inactivated above 50øC, 10 min
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using ammonium sulfate fractionation, ion exchange chromatography, affinity chromatography, and gel filtration
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using streptomycin sulfate treatment, Sephadex G-25 filtration, DEAE-Sepharose CL-6B chromatography, Sephadex G-150 filtration, Sepharose 6B filtration and hydroxyapatite chromatography
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synthesis
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ethyl (2R,3S)-3-hydroxy-2-methylbutanoate is a useful starting material for the synthesis of ferroelectric liquid crystal compounds and various other biologically active substances
synthesis
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ethyl (2R,3S)-3-hydroxy-2-methylbutanoate is a useful starting material for the synthesis of ferroelectric liquid crystal compounds and various other biologically active substances
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Heidlas, J.; Engel, K.H.; Tressl, R.
Purification and characterization of two oxidoreductases involved in the enantioselective reduction of 3-oxo, 4-oxo and 5-oxo esters in bakers yeast
Eur. J. Biochem.
172
633-639
1988
Saccharomyces cerevisiae
brenda
Miya, H.; Kawada, M.; Sugiyama, Y.
Purification and Characterization of Ethyl 2-Methyl-3-oxobutanoate Reductase from Klebsiella pneumoniae IF03319.
Biosci. Biotechnol. Biochem.
60
760-764
1996
Klebsiella pneumoniae, Klebsiella pneumoniae IFO3319
brenda
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