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Information on EC 1.1.1.271 - GDP-L-fucose synthase
for references in articles please use BRENDA:EC1.1.1.271
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EC Tree 1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.1.1 With NAD+ or NADP+ as acceptor
1.1.1.271 GDP-L-fucose synthase
IUBMB Comments
Both human and Escherichia coli enzymes can use NADH in place of NADPH to a slight extent.
The enzyme appears in viruses and cellular organisms
- 1.1.1.271
-
fucosylation
- gdp-d-mannose
-
4,6-dehydratase
-
k-dependent
- gdp-d-mannose-4,6-dehydratase
-
nadph-binding
- synthesis
showSynonyms
tsta3, fx protein, gdp-l-fucose synthase, gdp-fucose synthetase, transplantation antigen p35b, gdp-4-keto-6-deoxy-d-mannose epimerase/reductase, gdp-4-keto-6-deoxymannose-3,5-epimerase-4-reductase, gdp-fucose synthase, gdp-4-keto-6-deoxy-d-mannose-3,5-epimerase-4-reductase, gdp-keto-6-deoxymannose 3,5-epimerase/4-reductase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
fcl
FX protein
GDP-4-keto-6-D-deoxymannose epimerase-reductase
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-
-
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GDP-4-keto-6-deoxy-D-mannose epimerase-reductase
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-
-
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GDP-4-keto-6-deoxy-D-mannose epimerase/reductase
GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase
GDP-4-keto-6-deoxy-mannose-3,5-epimerase-4-reductase
GDP-4-keto-6-deoxymannose 3,5-epimerase 4-reductase
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-
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GDP-4-keto-6-deoxymannose epimerase-reductase
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-
-
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GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase
GDP-D-mannose-4,6-dehydratase
GDP-fucose synthase
GDP-fucose synthetase
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-
-
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GDP-keto-6-deoxymannose 3,5-epimerase/4-reductase
GDP-L-fucose synthetase
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-
-
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GDPFuc synthase
GER
GFS
GMER
guanosine diphosphofucose synthetase
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-
-
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P35B tumour rejection antigen
Red cell NADP(H)-binding protein
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Transplantation antigen P35B
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Tum-P35B antigen
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wcaG
FX protein
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GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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redox reaction
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-
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reduction
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
GDP-beta-L-fucose:NADP+ 4-oxidoreductase (3,5-epimerizing)
Both human and Escherichia coli enzymes can use NADH in place of NADPH to a slight extent.
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CAS REGISTRY NUMBER
COMMENTARY
113756-18-6
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
Substrates: fucosylation is regulated by complicated mechanisms that involve several factors: fucosyltransferases, GDP-fucose transporter, and GDP-fucose-synthetic enzymes, such as GDP-mannose 4,6-dehydratase, GDP-4-keto-6-deoxy-mannose-3,5-epimerase-4-reductase, and GDP-fucose pyrophosphorylase, fucose metabolism, overview
Products: -
Products: -
?
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADH + H+
GDP-beta-L-fucose + NAD+
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Substrates: -
Products: -
Products: -
r
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADH + H+
GDP-beta-L-fucose + NAD+
Substrates: the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4
Products: product identification by capillary electrophoresis, electro-spray ionization-mass spectrometry, and nuclear magnetic resonance spectroscopy
Products: product identification by capillary electrophoresis, electro-spray ionization-mass spectrometry, and nuclear magnetic resonance spectroscopy
?
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADH + H+
GDP-beta-L-fucose + NAD+
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Substrates: -
Products: -
Products: -
r
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+
GDP-beta-L-fucose + NADP+
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Substrates: the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4
Products: -
Products: -
?
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+
GDP-beta-L-fucose + NADP+
Substrates: -
Products: -
Products: -
?
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+
GDP-beta-L-fucose + NADP+
Substrates: the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4
Products: product identification by capillary electrophoresis, electro-spray ionization-mass spectrometry, and nuclear magnetic resonance spectroscopy
Products: product identification by capillary electrophoresis, electro-spray ionization-mass spectrometry, and nuclear magnetic resonance spectroscopy
?
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-L-fucose + NADP+
-
Substrates: -
Products: -
Products: -
r
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-L-fucose + NADP+
-
Substrates: the enzyme is crucial in the biosynthesis of GDP-L-fucose together with GDP-D-mannose-4,6-dehydratase, overview
Products: -
Products: -
r
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-L-fucose + NADP+
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Substrates: -
Products: -
Products: -
r
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-L-fucose + NADP+
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Substrates: the enzyme is required for the formation of activated L-fucose
Products: -
Products: -
r
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
Substrates: -
Products: -
Products: -
?
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
Substrates: -
Products: -
Products: -
?
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
Substrates: -
Products: -
Products: -
?
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
Substrates: -
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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Substrates: bifunctional enzyme that exhibits epimerase and reductase activity: 3,5-epimerization to GDP-4-keto-6-deoxy-L-galactose and subsequent NADPH-dependent reduction to GDP-L-fucose
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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Substrates: catalyzes the last two steps in the de novo synthesis of L-fucose
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
Substrates: -
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
Substrates: -
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
-
Substrates: -
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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Substrates: catalyzes the stereospecific hydride transfer of the ProS hydrogen from NADPH to carbon 4 of the mannose sugar
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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Substrates: enzyme also utilizes NADH but with lower affinity
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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Substrates: -
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
-
Substrates: -
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
-
Substrates: -
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
-
Substrates: -
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
-
Substrates: -
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
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Substrates: enzyme uses NADH as cofactor
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH + H+
GDP-L-fucose + NADP+
Substrates: -
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH + H+
GDP-L-fucose + NADP+
-
Substrates: -
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+
GDP-beta-L-fucose + NADP+
Substrates: -
Products: -
Products: -
?
GDP-4-keto-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-L-galactose + NADP+
-
Substrates: catalyzes the last two steps in the de novo synthesis of L-fucose
Products: -
Products: -
?
additional information
?
-
-
Substrates: fucosylation is regulated by complicated mechanisms that involve several factors: fucosyltransferases, GDP-fucose transporter, and GDP-fucose-synthetic enzymes, such as GDP-mannose 4,6-dehydratase, GDP-4-keto-6-deoxy-mannose-3,5-epimerase-4-reductase, and GDP-fucose pyrophosphorylase, fucose metabolism, overview
Products: -
Products: -
?
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-L-fucose + NADP+
-
Substrates: the enzyme is crucial in the biosynthesis of GDP-L-fucose together with GDP-D-mannose-4,6-dehydratase, overview
Products: -
Products: -
r
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-L-fucose + NADP+
-
Substrates: the enzyme is required for the formation of activated L-fucose
Products: -
Products: -
r
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
Substrates: -
Products: -
Products: -
?
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
Substrates: -
Products: -
Products: -
?
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
GDP-beta-L-fucose + NADP+
Substrates: -
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
both NADH and NADPH may be used as the cofactor by the enzyme, which shows similar affinities to NADH and NADPH, but the Vmax value is higher for NADPH
-
additional information
-
both NADH and NADPH may be used as the cofactor by the enzyme, which shows similar affinities to NADH and NADPH, but the Vmax value is higher for NADPH
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
6-alkynyl-fucose
competitive inhibitor, potently and selectively inhibits the enzyme
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GDP-fucose
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competitive with respect to NADPH and GDP-4-keto-6-deoxymannose
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Breast Neoplasms
Oncogenic potential of TSTA3 in breast cancer and its regulation by the tumor suppressors miR-125a-5p and miR-125b.
Breast Neoplasms
Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.
Carcinogenesis
Oncogenic potential of TSTA3 in breast cancer and its regulation by the tumor suppressors miR-125a-5p and miR-125b.
Carcinogenesis
Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.
Carcinoma
High TSTA3 Expression as a Candidate Biomarker for Poor Prognosis of Patients With ESCC.
Carcinoma
Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.
Carcinoma
TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2.
Carcinoma, Hepatocellular
An Alkynyl-Fucose Halts Hepatoma Cell Migration and Invasion by Inhibiting GDP-Fucose-Synthesizing Enzyme FX, TSTA3.
Carcinoma, Hepatocellular
Expression of genes that control core fucosylation in hepatocellular carcinoma: Systematic review.
Carcinoma, Hepatocellular
Tissue-specific transplantation antigen P35B (TSTA3) immune response-mediated metabolism coupling cell cycle to postreplication repair network in no-tumor hepatitis/cirrhotic tissues (HBV or HCV infection) by biocomputation.
Esophageal Squamous Cell Carcinoma
High TSTA3 Expression as a Candidate Biomarker for Poor Prognosis of Patients With ESCC.
Esophageal Squamous Cell Carcinoma
Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.
Esophageal Squamous Cell Carcinoma
TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2.
Insulin Resistance
Significant associations among hemostatic parameters, adipokines, and components of the metabolic syndrome in Japanese preschool children.
Lung Neoplasms
Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.
Lymphatic Metastasis
High TSTA3 Expression as a Candidate Biomarker for Poor Prognosis of Patients With ESCC.
Multiple Sclerosis
GDP-l-fucose synthase is a CD4+ T cell-specific autoantigen in DRB3*02:02 patients with multiple sclerosis.
Neoplasm Metastasis
High TSTA3 Expression as a Candidate Biomarker for Poor Prognosis of Patients With ESCC.
Neoplasm Metastasis
TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2.
Neoplasms
Expression of genes that control core fucosylation in hepatocellular carcinoma: Systematic review.
Neoplasms
Oncogenic potential of TSTA3 in breast cancer and its regulation by the tumor suppressors miR-125a-5p and miR-125b.
Neoplasms
TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2.
Osteonecrosis
ARG2, MAP4K5 and TSTA3 as Diagnostic Markers of Steroid-Induced Osteonecrosis of the Femoral Head and Their Correlation With Immune Infiltration.
Tuberculosis
Deciphering the genetic bases of the structural diversity of phenolic glycolipids in strains of the Mycobacterium tuberculosis complex.
Virus Diseases
Paramecium bursaria Chlorella virus 1 encodes two enzymes involved in the biosynthesis of GDP-L-fucose and GDP-D-rhamnose.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.047
GDP-4-dehydro-6-deoxy-alpha-D-mannose
recombinant His-tagged enzyme, pH 8.0, 37Ā°C
additional information
additional information
-
Km values for various mutants
-
additional information
additional information
Michaelis-Menten kinetiic modeling
-
additional information
additional information
-
Michaelis-Menten kinetiic modeling
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
values for various mutants
-
1
GDP-6-deoxy-4-keto-mannose
-
mutant C109S, isotope [5''-2H]-GDP-6-deoxy-4-keto-mannose
1
GDP-6-deoxy-4-keto-mannose
-
wild-type, isotope [3''-2H]-GDP-6-deoxy-4-keto-mannose
1
GDP-6-deoxy-4-keto-mannose
-
wild-type, isotope [5''-2H]-GDP-6-deoxy-4-keto-mannose
1.5
GDP-6-deoxy-4-keto-mannose
-
mutant C109S, isotope [3''-2H]-GDP-6-deoxy-4-keto-mannose
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Mycobacterium bovis
due to a natural mutation deleting the gene encoding the enzyme
-
-
brenda
syncytia induced by nematode Heterodera schachtii
UniProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
increased enzyme expression compared with adjacent tissue or the tissue of healthy livers, quantitative RT-PCR expression analysis, overview
brenda
the enzyme is aberrantly upregulated in human hepatoma
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
drug target
6-alkynyl-fucose effectively suppresses invasion of hepatoma cells, particularly for Fuc-high hepatoma, suggesting that this fucose analog can be used to suppress cancer metastasis by blocking aberrant fucosylation in cancer cells
physiological function
GDP-L-fucose synthase is an CD4+ T cell-specific autoantigen in DRB3*02:02 multiple sclerosis patients
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PDB
SCOP
CATH
UNIPROT
ORGANISM