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The enzyme from the bacterium Escherichia coli is specific for 5-dehydro-D-gluconate. cf. EC 220.127.116.116, L-idonate 5-dehydrogenase (NAD+).
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
idonate 5-dehydrogenase, L-IdnDH, L-idonate dehydrogenase, more
L-idonate + NAD(P)+ = 5-dehydro-D-gluconate + NAD(P)H + H+
The enzyme from E. coli cannot oxidize D-gluconate to form 5-dehydrogluconate, a reaction that is catalysed by EC 18.104.22.168, gluconate 5-dehydrogenase
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