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IUBMB CommentsActs on a number of bile acids, both in their free and conjugated forms.
The expected taxonomic range for this enzyme is: Clostridium paraputrificum
Synonyms
12beta-hydroxy steroid (nicotinamide adenine dinucleotide phosphate) dehydrogenase, more
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12beta-hydroxy steroid (nicotinamide adenine dinucleotide phosphate) dehydrogenase
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3alpha,7alpha,12beta-trihydroxy-5beta-cholan-24-oate + NADP+ = 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate + NADPH + H+
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MetaCyc
bile acids 12-O-epimerization
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12beta-hydroxysteroid:NADP+ 12-oxidoreductase
Acts on a number of bile acids, both in their free and conjugated forms.
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3,12-dioxo-5beta-cholanoate + NADPH
12beta-hydroxy-3-oxo-5beta-cholanoate + NADP+
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3,7,12-trioxo-5beta-cholanoate + NADPH
12beta-hydroxy-3,7-dioxo-5beta-cholanoate + NADP+
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3,7,12-trioxotaureate + NADPH
12beta-hydroxy-3,7-dioxotaureate + NADP+
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3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate + NADPH
3alpha,7alpha,12beta-trihydroxy-5beta-cholanoate + NADP+
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3alpha-hydroxy-12-oxo-5beta-cholanoate + NADPH
3alpha,12beta-dihydroxy-5beta-cholanoate + NADP+
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Substrates: reverse reaction 10% the rate of forward reaction
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3alpha-hydroxy-7,12-dioxo-5beta-cholanoate + NADPH
3alpha,12beta-dihydroxy-7-oxo-5beta-cholanoate + NADP+
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7,12-dioxo-5beta-cholanoate + NADPH
12beta-hydroxy-7-oxo-5beta-cholanoate + NADP+
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7alpha-hydroxy-3,12-dioxo-5beta-cholanoate + NADPH
7alpha,12beta-dihydroxy-3-oxo-5beta-cholanoate + NADP+
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NADPH
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specific for, no activity with NADH
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0.0895
3,12-Dioxo-5beta-cholanoate
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0.171
3,7,12-Trioxo-5beta-cholanoate
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0.142
3,7,12-trioxotaureate
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0.274
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate
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0.141
3alpha-Hydroxy-12-oxo-5beta-cholanoate
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0.144
3alpha-Hydroxy-7,12-dioxo-5beta-cholanoate
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0.0837
7,12-Dioxo-5beta-cholanoate
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0.138
7alpha-Hydroxy-3,12-dioxo-5beta-cholanoate
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2 - 4.4
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depending on substrate
2.2 - 6.4
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depending on inducer
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10
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reduction of 12-oxo bile acids
7.8
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oxidation of 12beta-hydroxy bile acids
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7 - 11
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about half maximum activity
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brenda
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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37
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45 min, complete inactivation
50
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10 min, 95% inactivation, 15 min complete inactivation
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most stable in presence of reducing agents e.g. dithiothreitol
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-20°C, lyophilized powder, at least 4 weeks
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analysis
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potential application in quantification of 12beta-hydroxyl groups in di- and trisubstituted bile acids
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Edenharder, R.; Pfutzner, A.
Characterization of NADP-dependent 12beta-hydroxysteroid dehydrogenase from Clostridium paraputrificum
Biochim. Biophys. Acta
962
362-370
1988
Clostridium paraputrificum
brenda
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