Information on EC 1.1.1.231 - 15-hydroxyprostaglandin-I dehydrogenase (NADP+)

for references in articles please use BRENDA:EC1.1.1.231
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:

The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
1.1.1.231
-
top print hide
RECOMMENDED NAME
GeneOntology No.
15-hydroxyprostaglandin-I dehydrogenase (NADP+)
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH + H+
show the reaction diagram
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate:NADP+ 15-oxidoreductase
Specific for prostaglandin I2.
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
79468-49-8
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
chinese hamster
-
-
Manually annotated by BRENDA team
monkey
-
-
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
show the reaction diagram
4-benzoylpyridine + NADP+
?
show the reaction diagram
-
recombinant forms CHCR1, CHCR2
-
-
?
4-nitrobenzaldehyde + NADP+
4-nitrobenzoate + NADPH
show the reaction diagram
6-keto-prostaglandin F1alpha + NADP+
6,15-ketoprostaglandin F2alpha + NADPH
show the reaction diagram
-
at 1.8% the reaction rate of prostaglandin I2
-
-
?
menadione + NADP+
?
show the reaction diagram
-
more rapid reaction than with prostacyclin
-
-
?
phenanthrene quinone + NADP+
?
show the reaction diagram
-
more rapid reaction than with prostacyclin
-
-
?
prostaglandin B1 + NADP+
15-ketoprostaglandin B1 + NADPH + H+
show the reaction diagram
-
at 8% the reaction rate of prostaglandin I2
-
-
?
prostaglandin E2 + NADP+
15-ketoprostaglandin E2 + NADPH + H+
show the reaction diagram
prostaglandin F2alpha + NAD+
15-ketoprostaglandin F2alpha + NADH + H+
show the reaction diagram
-
-
-
-
?
prostaglandin F2alpha + NADP+
15-ketoprostaglandin F2alpha + NADPH + H+
show the reaction diagram
pyridine-4-carboxaldehyde + NADP+
?
show the reaction diagram
-
recombinant forms CHCR1, CHCR2
-
-
?
additional information
?
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
show the reaction diagram
-
-
-
-
?
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Furosemide
-
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0667
4-Nitrobenzaldehyde
-
-
0.25
menadione
-
-
0.00538 - 0.0134
NADP+
0.0005
Phenanthrene quinone
-
-
0.278 - 0.364
prostaglandin I2
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.217
-
recombinant enzyme, latanoprost + NADP+
2.17
-
recombinant enzyme, acid form of latanoprost + NADP+
6.08
-
recombinant enzyme, prostaglandin F2alpha + NAD+
9.8
-
recombinant enzyme, prostaglandin E2 + NADP+
24.3
-
recombinant enzyme, prostaglandin F2alpha + NADP+
additional information
-
-
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
tissue distribution of m-RNA
Manually annotated by BRENDA team
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
2 * 28000, rabbit, SDS-PAGE
31000
-
SDs-PAGE of recombinant enzyme
34000
-
SDS-PAGE of recombinant forms CHCR1, CHCR2 including His-tag
56000
-
SDS-PAGE of amidinated enzyme
62000
-
gel filtration
additional information
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no modification
-
-
top print hide
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing: repeatedly, slow loss of activity
-
top print hide
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
stable for months when frozen
-
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 forms differing due to posttranslational modification at pos. 238
-
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 enzyme forms CHCR1, CHCR2
-
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.231)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)