We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Also acts, more slowly, on (2Z,6E)-farnesol, geraniol, citronerol and nerol.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
sdr-1, nad(p)+-dependent farnesol dehydrogenase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dehydrogenase, farnesol (nicotinamide adenine dinucleotide phosphate)
-
-
-
-
NAD(P)+-dependent farnesol dehydrogenase
-
-
NADP+-dependent farnesol dehydrogenase 1
-
NADP-farnesol dehydrogenase
-
-
-
-
additional information
-
cf. EC 1.1.1.354
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2E,6E)-farnesol + NADP+ = (2E,6E)-farnesal + NADPH + H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2E,6E)-farnesol:NADP+ 1-oxidoreductase
Also acts, more slowly, on (2Z,6E)-farnesol, geraniol, citronerol and nerol.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2E,6E)-farnesol + NAD+
(2E,6E)-farnesal + NADH + H+
-
preferred substrates
-
-
?
(2E,6E)-farnesol + NADP+
(2E,6E)-farnesal + NADPH + H+
(2Z,6E)-farnesol + NAD+
(2Z,6E)-farnesal + NADH + H+
-
66% of the activity with 2-trans,6-trans-farnesol
-
-
?
(2Z,6Z)-farnesol + NAD+
(2Z,6Z)-farnesal + NADH + H+
-
47% of the activity with 2-trans,6-trans-farnesol
-
-
?
(2Z,6Z)-farnesol + NADP+
(2Z,6Z)-farnesal + NADPH + H+
enzyme shows highest affinity for farnesol
-
-
r
(E)-geraniol + NADP+
(E)-geranial + NADPH + H+
-
-
-
?
(S)-perillyl alcohol + NAD+
?
-
34% of the activity with 2-trans,6-trans-farnesol
-
-
?
(Z)-nerol + NADP+
(Z)-neral + NADPH + H+
-
-
-
?
2-decanol + NADP+
? + NADPH + H+
-
-
-
?
carveol + NAD+
?
-
32% of the activity with 2-trans,6-trans-farnesol
-
-
?
cinnamyl alcohol + NAD+
?
-
35% of the activity with 2-trans,6-trans-farnesol
-
-
?
cis,trans-farnesol + NADP+
cis,trans-farnesal + NADPH
-
-
-
?
citronellol + NADP+
?
-
-
-
-
?
citronellol + NADP+
citronellal + NADPH + H+
-
-
-
?
decanol + NADP+
decanal + NADPH
-
-
-
-
?
farnesol + NADP+
farnesal + NADPH
-
-
-
?
geraniol + NAD+
?
-
37% of the activity with 2-trans,6-trans-farnesol
-
-
?
geraniol + NADP+
?
-
-
-
-
?
nerodilol + NAD+
?
-
36% of the activity with 2-trans,6-trans-farnesol
-
-
?
nerol + NADP+
?
-
-
-
-
?
octanol + NADP+
octanal + NADPH + H+
-
-
-
?
trans,trans-farnesol + NADP+
trans,trans-farnesal + NADPH
-
preferred substrate
-
r
additional information
?
-
(2E,6E)-farnesol + NADP+
(2E,6E)-farnesal + NADPH + H+
enzyme shows highest affinity for farnesol
-
-
ir
(2E,6E)-farnesol + NADP+
(2E,6E)-farnesal + NADPH + H+
-
NADP+ show 22% of the activity with NAD+
-
-
?
(2E,6E)-farnesol + NADP+
(2E,6E)-farnesal + NADPH + H+
-
-
-
-
?
(2E,6E)-farnesol + NADP+
(2E,6E)-farnesal + NADPH + H+
-
-
-
-
?
additional information
?
-
no activity is detected for butanol, ethanol, or glycerol, or for farnesal or farnesoic acid
-
-
?
additional information
?
-
-
trans,trans-farnesol is the preferred substrate for the Polygonum minus farnesol dehydrogenase, the geometrical isomers cis,trans-farnesol and cis,cis-farnesol are also oxidized by the enzyme with lower activity. No activity with nerol, p-cumic alcohol, borneol, linalool, menthol, beta-citronellol, 3,7-dihydrolinalool, benzyl alcohol, 2,4-octadien-1-ol, or 2,5-dimethyl-1.5-hexadien-3-ol, tetrahydrogeraniol, tetrahydrolinalool, tetrahydrolavandulol, ethanol, and methanol. Substrate specificity, overview
-
-
?
additional information
?
-
-
enzyme is highly specific for trans, trans-farnesol, and NADP+
-
-
-
additional information
?
-
-
(2E,6E)-farnesol is the most preferred substrate
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
farnesol + NADP+
farnesal + NADPH
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
the enzyme utilizes both NAD+ and NADP+ with a strong preference for NAD+
-
NADP+
-
-
NADP+
-
NAD+ cannot replace NADP+
NADP+
absolutely required, NAD+ or FAD cannot substitute
NADP+
-
highly specific for NADP+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,2-dipyridyl
-
52% inhibition at 1 mM
5,5'-dithiobis(2-nitrobenzoic acid)
-
complete inhibition at 1 mM
Ag+
-
complete inhibition at 1 mM
Ca2+
-
24% inhibition at 1 mM
Fe3+
-
complete inhibition at 1 mM
iodoacetamide
-
66% inhibition at 1 mM
Li+
-
complete inhibition at 1 mM
Mg2+
-
73% inhibition at 1 mM
p-chloromercuribenzoate
-
complete inhibition at 1 mM
Sodium azide
-
25% inhibition at 1 mM
Zn2+
-
complete inhibition at 1 mM
1,10-phenanthroline
-
weak irreversible inhibitor
1,10-phenanthroline
-
complete inhibition at 1 mM
Cu2+
-
-
Cu2+
-
complete inhibition at 1 mM
EDTA
-
-
EDTA
-
complete inhibition at 1 mM
additional information
-
inhibitory effect of thiol reagents indicates that the SH-group of the enzyme participates in its activity
-
additional information
-
the enzyme activity is inhibited by sulfhydryl reagents, metal-chelating agents and heavy metal ions
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
FAD
-
slightly enhances activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.091 - 0.17
(2E,6E)-farnesol
0.33
(2Z,6E)-farnesol
-
pH 9.5, 35°C
0.097 - 0.42
(2Z,6Z)-farnesol
0.208
(E)-geraniol
pH 10.0, 30°C
0.77
(S)-perillyl alcohol
-
pH 9.5, 35°C
0.109
(Z)-nerol
pH 10.0, 30°C
0.184
2-decanol
pH 10.0, 30°C
0.71
carveol
-
pH 9.5, 35°C
1.04
cinnamyl alcohol
-
pH 9.5, 35°C
0.133
citronellol
pH 10.0, 30°C
1
nerodilol
-
pH 9.5, 35°C
0.195
Octanol
pH 10.0, 30°C
0.0156
trans,trans-farnesol
-
-
additional information
additional information
-
Michaelis-Menten kinetics
-
0.091
(2E,6E)-farnesol
pH 10.0, 30°C
0.17
(2E,6E)-farnesol
-
pH 9.5, 35°C
0.097
(2Z,6Z)-farnesol
pH 10.0, 30°C
0.42
(2Z,6Z)-farnesol
-
pH 9.5, 35°C
0.729
geraniol
-
-
12.5
geraniol
-
pH 9.5, 35°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00002
farnesyl acetate
-
pH not specified in the publication, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.27
-
purified native enzyme, pH 9.5, 35°C
9
substrate (2Z,6Z)-farnesol, pH 10.0, 30°C
9.7
substrate (2E,6E)-farnesol, pH 10.0, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7 - 7.5
-
reduction of trans,trans-farnesal
9.5
-
oxidation of trans,trans-farnesol
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6 - 9
-
reduction of trans,trans-farnesal: 60% of maximal activity at pH 6.5 and at pH 8
7 - 10.5
-
oxidation of trans,trans-farnesol: 10% of maximal activity at pH 7.0, 30% of maximal activity at pH 10.2
7.5 - 11
-
activity range, profile overview. At below pH 8.5 or above pH 10.0, the enzyme activity declines sharply
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
25 - 60
-
activity range, profile overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.8
-
isoelectric focusing
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NADP+-dependent farnesol dehydrogenase 1
UniProt
brenda
sweet potato, enzyme is formed in response to infection by the black rot fungus Ceratocystis fimbriata
-
-
brenda
tobacco hornworm
-
-
brenda
plants growing in an experimental plot at the Institute of Systems Biology of Universiti Kebangsaan Malaysia
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
and midgut, of adult female, highest levels of mRNA
brenda
no farnesol dehydrogenase activity in newly emerged mosquitoes but significant activity 24 h later. SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis
brenda
-
-
brenda
and Malpighian tubules, considerable levles of mRNA
brenda
abdominal and thoracic ganglia, high level of mRNA
brenda
-
-
brenda
-
-
brenda
and fat body, considerable levles of mRNA
brenda
and brain, of adult female, highest levels of mRNA
brenda
-
-
brenda
of adult male, substantial level of mRNA
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
the enzyme is involved in the biosynthesis of precursors that can be used for the biosynthetic pathway of juvenile hormone III (JH III, methyl-10R,11-epoxy-3,7,11-trimethyl-2E,6E-dodecadienoate). Deployment of transgenic plant with farnesol dehydrogenase enzyme will be beneficial for use in manipulating juvenile hormone biosynthesis in plants
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SDR1_AEDAE
245
0
26542
Swiss-Prot
other Location (Reliability: 3 )
AK1BF_HUMAN
316
0
36537
Swiss-Prot
other Location (Reliability: 2 )
AK1CA_RAT
324
0
37195
Swiss-Prot
other Location (Reliability: 3 )
A0A2G9HQY6_9LAMI
171
0
18696
TrEMBL
other Location (Reliability: 4 )
A0A7X0CPF1_9SPHI
362
0
40451
TrEMBL
-
A0A0B8N8C3_9NOCA
294
0
31479
TrEMBL
-
A0A369QPA4_9BACT
214
0
23579
TrEMBL
-
A0A841JEI2_9SPHI
356
0
39897
TrEMBL
-
A0A2R2WS82_9CUCU
247
0
26801
TrEMBL
other Location (Reliability: 4 )
A0A4Y8AE63_9SPHI
329
0
36388
TrEMBL
-
A0A2I0B436_9ASPA
592
0
64371
TrEMBL
Chloroplast (Reliability: 4 )
D2WKE0_AEDAE
245
0
26713
TrEMBL
other Location (Reliability: 3 )
D2WKE1_AEDAE
244
0
26577
TrEMBL
other Location (Reliability: 2 )
A0A2I0B7N1_9ASPA
338
0
36999
TrEMBL
Mitochondrion (Reliability: 4 )
A0A7W8KYF0_9SPHI
326
0
36489
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
29000
2 * 29000, calculated
47000
-
2 * 47000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
heterodimer
-
1 * 65000 + 1 * 70000, SDS-PAGE
additional information
-
tryptic peptide mapping and MALDI-TOF/TOF-MS spectrometric analysis, sequence comparisons, overview
dimer
2 * 29000, calculated
dimer
-
2 * 47000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
30 - 65
-
purified native enzyme, 10 min at pH 7.5 in 100 mM tricine-NaOH containing 2.5 mM 2-mercaptoethanol, completely stable up to 30°C, over 90% activity up to 55°C, loss of 80% activity at 60°C, loss of 85% activity at 65°C, and inactivation at 70°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
native enzyme 234fold from leaves to apparent homogeneity by two different steps of anion-exchange chromatography and a cation exchange chromatography step, followed by three successive steps of gel filtration
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
agriculture
-
recombinant farnesol dehydrogenase may provide a useful molecular tool in manipulating juvenile hormone biosynthesis to generate transgenic plants for pest control
biotechnology
-
recombinant farnesol dehydrogenase may provide a useful molecular tool in manipulating juvenile hormone biosynthesis to generate transgenic plants for pest control
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Inoue, H.; Tsuji, H.; Uritani, I.
Characteriziation and activity change of farnesol dehydrogenase in black rot fungus-infected sweet potato
Agric. Biol. Chem.
48
733-738
1984
Ipomoea batatas
-
brenda
Sperry, A.E.; Sen, S.E.
Farnesol oxidation in insects: evidence that the biosynthesis of insect juvenile hormone is mediated by a specific alcohol oxidase
Insect Biochem. Mol. Biol.
31
171-178
2001
Manduca sexta
brenda
Mayoral, J.G.; Nouzova, M.; Navare, A.; Noriega, F.G.
NADP+-dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis
Proc. Natl. Acad. Sci. USA
106
21091-21096
2009
Aedes aegypti (D2WKD9)
brenda
Ahmad-Sohdi, N.A.; Seman-Kamarulzaman, A.F.; Mohamed-Hussein, Z.A.; Hassan, M.
Purification and characterization of a novel NAD(P)+-farnesol dehydrogenase from Polygonum minus leaves
PLoS ONE
10
e0143310
2015
Persicaria minor
brenda
Zifruddin, A.N.; Mohamad-Khalid, K.A.; Suhaimi, S.A.; Mohamed-Hussein, Z.A.; Hassan, M.
Molecular characterization and enzyme inhibition studies of NADP+-farnesol dehydrogenase from diamondback moth, Plutella xylostella (Lepidoptera Plutellidae)
Biosci. Biotechnol. Biochem.
85
1628-1638
2021
Plutella xylostella
brenda
Satyaveanthan, M.; Suhaimi, S.; Ng, C.; Muhd-Noor, N.; Awang, A.; Lam, K.; Hassan, M.
Purification, biochemical characterisation and bioinformatic analysis of recombinant farnesol dehydrogenase from Theobroma cacao
Plant Physiol. Biochem.
161
143-155
2021
Theobroma cacao
brenda
Select items on the left to see more content.
html completed