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(+)-camphorquinone + NADPH
? + NADP+
(-)-camphorquinone + NADPH
? + NADP+
-
relative activity 2%
-
-
?
(2E)-4-hydroxynon-2-enal + NADPH
(2E)-non-2-ene-1,4-diol + NADP+
-
30% of activity with cinnamaldehyde
-
-
r
(2E)-hex-2-enal + NADPH + H+
(2E)-2-hexen-1-ol + NADP+
-
-
-
-
r
(2E,4E)-hexa-2,4-dienal + NAD(P)H + H+
(2E,4E)-6-hydroxyhexa-2,4-dienal + NAD(P)+
-
-
-
-
-
(2R,3R)-2,3-butanediol + NADP+
(R)-2-acetoin + NADPH + H+
the enzyme shows higher oxidation activities on the (2R,3R)-(-)-2,3-butanediol and meso-2,3-butanediol than on the (2S,3S)-(+)-2,3-butanediol, indicating that the enzyme predominantly oxidizes the R-hydroxyl group of 2,3-butanediol and minorly functions on the S-hydroxyl group. When meso-2,3-butanediol is oxidized, (S)-2-acetoin is the predominant product, with an enantiomeric excess (ee) of 88%, while oxidation of (2R,3R)-2,3-butanediol results in the production of (R)-2-acetoin with higher stereoselectivity (94% ee)
-
-
r
(2S)-methyl-1-butanol + NADP+
(2S)-methylbutanal + NADPH
-
39% activity compared to cinnamyl alcohol
-
-
r
(2S,3S)-2,3-butanediol + NADP+
(S)-2-acetoin + NADPH + H+
the enzyme shows higher oxidation activities on the (2R,3R)-(-)-2,3-butanediol and meso-2,3-butanediol than on the (2S,3S)-(+)-2,3-butanediol, indicating that the enzyme predominantly oxidizes the R-hydroxyl group of 2,3-butanediol and minorly functions on the S-hydroxyl group. When meso-2,3-butanediol is oxidized, (S)-2-acetoin is the predominant product, with an enantiomeric excess (ee) of 88%, while oxidation of meso-2,3-butanediol results in the production of (R)-2-acetoin with higher stereoselectivity (94% ee)
-
-
r
(4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzaldehyde) + NADPH + H+
(4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzyl alcohol) + NADP+
-
eutypine, a toxin produced by Eutypa lata, the causal agent of Eutypa dieback in the grapevine Vitis vinifera
eutypinol, non-toxic metabolite
ir
(R)-1-phenyl-2-propanol + NADP+
phenylacetone + NADPH + H+
-
-
-
-
?
(R)-2-butanol + NADP+
2-butanone + NADPH + H+
the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol
-
-
?
(S)-1-phenyl-2-propanol + NADP+
phenylacetone + NADPH + H+
-
-
-
-
r
(S)-2-butanol + NADP+
2-butanone + NADPH + H+
the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol
-
-
?
(S)-2-butanol + NADP+
butanone + NADPH + H+
-
196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol
-
-
?
1,2-butanediol + NADP+
? + NADPH + H+
25.6% of the activity compared to the substrate 2-butanol
-
-
?
1,2-cyclohexanedione + NADPH
? + NADP+
-
-
-
-
?
1,2-propanediol + NADP+
? + NADPH
-
-
-
-
?
1,3-butanediol + NADP+
? + NADPH
-
-
-
-
?
1,3-butanediol + NADP+
? + NADPH + H+
35.4% of the activity compared to the substrate 2-butanol
-
-
?
1,3-propanediol + NADP+
?
-
-
-
-
?
1,3-propanediol + NADP+
? + NADPH
1,4-butanediol + NADP+
?
-
-
-
-
?
1,5-pentanediol + NADP+
?
-
-
-
-
?
1,5-pentanediol + NADP+
? + NADPH + H+
1-butanol + NADP+
butanal + NADPH
1-butanol + NADP+
butanal + NADPH + H+
1-butanol + NADP+
butyraldehyde + NADPH + H+
1-heptanol + NADP+
heptanal + NADPH + H+
1-hexanol + NADP+
hexanal + NADPH
1-hexanol + NADP+
hexanal + NADPH + H+
1-octanol + NADP+
octanal + NADPH
1-octanol + NADP+
octanal + NADPH + H+
1-pentanol + NADP+
n-pentanal + NADPH
1-pentanol + NADP+
n-pentanal + NADPH + H+
-
-
-
-
?
1-pentanol + NADP+
pentanal + NADPH
1-pentanol + NADP+
pentanal + NADPH + H+
1-phenyl-2-butanone + NADPH + H+
(S)-1-phenyl-2-butanol + NADP+
-
-
-
-
?
1-propanol + NADP+
?
-
-
-
-
?
1-propanol + NADP+
propanal + NADPH
-
16% activity compared to cinnamyl alcohol
-
-
r
1-propanol + NADP+
propanal + NADPH + H+
13-cis-retinal + NADPH
13-cis-retinol + NADP+
-
-
-
-
?
2,2,2-trifluoro-1-phenylethanone + NADPH + H+
(1S)-2,2,2-trifluoro-1-phenylethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2,2-difluoro-1-phenylethanone + NADPH + H+
(1S)-2,2-difluoro-1-phenylethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2,3-pentanedione + NADPH + H+
? + NADP+
2,4-hexadienal + NADPH
2,4-hexadien-1-ol + NADP+
-
-
-
-
?
2,4-pentanediol + NADP+
? + NADPH + H+
9.2% of the activity compared to the substrate 2-butanol
-
-
?
2,5-hexanedione + NADP+
(2S,5S)-hexanediol + NADPH + H+
-
-
-
-
r
2-(S)-methylbutanol + NADP+
(2S)-2-methylbutanal + NADPH
-
7% of activity with cinnamyl alcohol
-
-
r
2-butanol + NADP+
2-butanone + NADPH
-
2% of activity with cinnamyl alcohol
-
-
r
2-butanol + NADP+
2-butanone + NADPH + H+
the enzyme does not possess stereoselectivity on 2-butanol or 2-butanone as substrate
-
-
r
2-butanol + NADP+
butan-2-one + NADPH
2-butanol + NADP+
butanone + NADPH
-
1% activity compared to cinnamyl alcohol
-
-
r
2-butanone + NADP+
2-butanol + NADPH + H+
-
-
-
-
r
2-butanone + NADPH + H+
2-butanol + NADP+
the enzyme does not possess stereoselectivity on 2-butanol or 2-butanone as substrate
-
-
r
2-chloroheptanal + NADPH
2-chloroheptanol + NADP+
-
-
-
-
r
2-decanone + NADPH
2-decanol + NADP+
-
-
-
-
?
2-fluoro-1-(4-chlorophenyl)ethanone + NADPH + H+
(1S)-2-fluoro-1-(4-chlorophenyl)ethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-fluoro-1-(4-fluorophenyl)ethanone + NADPH + H+
(1S)-2-fluoro-1-(4-fluorophenyl)ethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-fluoro-1-(4-iodophenyl)ethanone + NADPH + H+
(1S)-2-fluoro-1-(4-iodophenyl)ethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-fluoro-1-phenylethanone + NADPH + H+
(1S)-2-fluoro-1-phenylethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-heptanol + NADP+
heptan-2-one + NADPH
-
-
-
-
r
2-methoxybenzaldehyde + NADPH + H+
2-methoxybenzyl alcohol + NADP+
2-methyl-1-propanol + NADP+
2-methylpropanal + NADPH
2-methylbutanal + NADPH
2-methyl-1-butanol + NADP+
2-methylbutanal + NADPH
2-methylbutanol + NADP+
-
-
-
-
r
2-methylbutanol + NADP+
2-methylbutyraldehyde + NADPH + H+
-
24% activity with 1 mM 2-methylbutanol, 22% activity with 10 mM 2-methylbutanol
-
-
r
2-methylbutanol + NADP+
? + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 24% relative activity with 1 mM or 22% relative activity with 10 mM substrate
-
-
?
2-methylpropanal + NADPH
2-methyl-1-propanol + NADP+
2-methylpropanol + NADP+
2-methylpropanal + NADPH
-
1% of activity with cinnamyl alcohol
-
-
r
2-methylundecanal + NADPH
2-methylundecanol + NADP+
-
-
-
-
?
2-pentanol + NADP+
2-pentanone + NADPH + H+
2-pentanol + NADP+
pentan-2-one + NADPH
-
-
-
-
r
2-pentanol + NADP+
pentan-2-one + NADPH + H+
-
67% of the activity with 2-propanol
-
-
?
2-pentanone + NADP+
2-pentanol + NADPH + H+
-
-
-
-
r
2-pentanone + NADPH + H+
2-pentanol + NADP+
86.2% of the activity compared to the substrate 2-butanone
-
-
r
2-phenylacetaldehyde + NADPH + H+
2-phenylethanol + NADP+
-
-
-
-
r
2-phenylethanol + NADP+
2-phenylacetaldehyde + NADPH + H+
2-phenylethanol + NADP+
phenylacetaldehyde + NADPH
-
23% activity compared to cinnamyl alcohol
-
-
r
2-phenylethanol + NADP+
phenylacetaldehyde + NADPH + H+
2-propanol + NADP+
acetone + NADPH
2-propanol + NADP+
acetone + NADPH + H+
2-propanone + NADP+
2-propanol + NADPH + H+
-
-
-
-
r
2-propen-1-ol + NADP+
acrylaldehyde + NADPH
-
-
-
-
r
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+
1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
3,4-dihydroxyphenylacetaldehyde + NADPH + H+
3,4-dihydroxyphenylethanol + NADP+
-
-
-
-
r
3,4-dihydroxyphenylglycolaldehyde + NADPH
? + NADP+
3-cyanobenzaldehyde + NADPH
3-cyanobenzyl alcohol + NADP+
-
-
-
-
?
3-deoxyglucosone + NADPH
? + NADP+
-
-
-
-
r
3-heptanone + NADPH
3-heptanol + NADP+
-
-
-
-
?
3-hexanol + NADP+
hexan-3-one + NADPH
-
-
-
-
r
3-methyl-1,5-pentanediol + NADP+
?
3-methyl-1-butanol + NADP+
3-methylbutanal + NADPH
-
40% activity compared to cinnamyl alcohol
-
-
r
3-methylbutanal + NADPH
3-mehtyl-1-butanol + NADP+
-
84% of activity with cinnamaldehyde
-
-
r
3-methylbutanal + NADPH
3-methyl-1-butanol + NADP+
-
79% activity compared to cinnamaldehyde
-
-
r
3-methylbutanal + NADPH
3-methylbutanol + NADP+
-
-
-
-
r
3-methylbutanol + NADP+
3-methylbutanal + NADPH
-
15% of activity with cinnamyl alcohol
-
-
r
3-octanone + NADPH
3-octanol + NADP+
-
-
-
-
?
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
3-pyridinemethanol + NADP+
3-pyridinecarboxaldehyde + NADPH
-
-
-
-
r
4-benzoylpyridine + ?
?
-
-
-
-
r
4-carboxyacetophenone + NADPH
? + NADP+
-
-
-
-
r
4-carboxyphenylglyoxal + NADPH
? + NADP+
-
-
-
-
r
4-hydroxy-3-methoxybenzyl alcohol + NADP+
4-hydroxy-3-methoxybenzaldehyde + NADPH
-
-
-
-
r
4-hydroxy-3-methoxyphenethylene glycol + NADP+
? + NADPH
-
-
-
-
r
4-hydroxybenzaldehyde + NADPH + H+
4-hydroxybenzyl alcohol + NADP+
-
-
-
-
?
4-hydroxybutyrate + NADP+
4-ketobutyrate + NADPH
-
-
-
-
r
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
4-methylbenzyl alcohol + NADP+
4-methylbenzaldehyde + NADPH
-
-
-
-
r
4-nitroacetophenone + NADPH
1-(4-nitrophenyl)ethanol + NADP+
-
-
-
-
?
4-phenyl-2-butanone + NADPH + H+
(S)-4-phenyl-2-butanol + NADP+
-
-
-
-
?
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+
(furan-2,5-diyl)dimethanol + NADP+
5-hydroxyindoleacetaldehyde + NADPH
? + NADP+
-
-
-
-
?
5-hydroxymethylfurfural + NADPH + H+
5-hydroxymethylfurfuryl alcohol + NADP+
5alpha-dihydrotestosterone + NADPH
? + NADP+
-
-
-
-
?
9-cis-retinal + NADPH
9-cis-retinol + NADP+
-
-
-
-
r
9-cis-retinol + NADP+
9-cis-retinal + NADPH
-
-
-
-
r
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly + NADH + H+
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly-al + NADPH + H+
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly-S-CoA + NADPH + H+
?
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu- + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe-ol + NADP+
-
-
-
-
-
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly- + NADH + H+
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly-al + NAD+ + H2O
-
-
-
-
-
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly-al + NADPH + H+
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly-ol + NADP+
-
-
-
-
-
acetaldehyde + NADPH + H+
ethanol + NADP+
acetoin + NADPH + H+
2,3-butanediol + NADP+
93% of the activity compared to the substrate 2-butanone. The racemic R/S-acetoin is used since neither R- nor S-acetoin is commercially available. Consistently, the reduction of racemic R/S-acetoin formed (2R,3R)-2,3-butanediol (presumably from R-acetoin) and meso-2,3-butanediol (presumably from S-acetoin) with extremely high stereoselectivity (99% ee over (2S,3S)-(+)-2,3-butanediol)
-
-
r
acetoin + NADPH + H+
butan-2,3-diol + NADP+
-
isozyme AR3
-
-
?
acetone + NADPH
2-propanol + NADP+
-
-
-
-
?
acetone + NADPH + H+
2-propanol + NADP+
149% of the activity compared to the substrate 2-butanone
-
-
r
acetone + NADPH + H+
propan-2-ol + NADP+
-
about 13% of the activity with butan-2-ol
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
recombinant mutant G37D
recombinate mutant G37D
-
r
acetophenone + NADPH
(R)-1-phenylethanol + NADP+
-
catalysed by Escherichia coli coexpressing enzyme with NAD+-dependent formate dehydrogenase and pyridine nucleotide transhydrogenase genes pnta and pntb
-
-
?
acetophenone + NADPH
1-phenylethanol + NADP+
-
-
-
-
?
acetophenone + NADPH + H+
(S)-1-phenylethanol + NADP+
-
-
-
-
r
acetophenone + NADPH + H+
1-phenylethanol + NADP+
-
recombinant wild-type
recombinant wild-type
-
r
acetyl-CoA + NADPH
acetaldehyde + CoA + NADP+
-
-
-
-
?
acrolein + NADPH
propenol + NADP+
-
-
-
-
r
adonitol + NADP+
? + NADPH
-
-
-
-
r
aldehyde + NADPH + H+
alcohol + NADP+
-
the physiological role of this enzyme is likely to catalyze the reduction of aldehydes to alcohols
-
-
?
all-trans-retinal + NADPH + H+
all-trans-retinol + NADP+
-
-
-
-
r
all-trans-retinol + NADP+
all-trans-retinal + NADPH
-
-
-
-
r
allactose + NADPH
? + NADP+
-
-
-
-
?
alpha-diketone + NADPH
? + NADP+
-
-
-
-
?
androstane-3,17-dione + NADPH
? + NADP+
-
-
-
-
?
anisaldehyde + NADPH
4-methoxybenzyl alcohol + NADP+
anisaldehyde + NADPH + H+
anisyl alcohol + NADP+
anisyl alcohol + NADP+
anisaldehyde + NADPH
-
-
-
-
r
benzalacetone + NADPH
? + NADP+
-
-
-
-
?
benzaldehyde + NADPH
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 30% relative activity with 0.1 mM or 14% relative activity with 1 mM substrate
-
-
r
benzoic aldehyde + NADPH + H+
? + NADP+
-
-
-
-
?
benzyl alcohol + NADP+
benzaldehyde + NADPH
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
butan-1-ol + NADP+
butanal + NADPH + H+
butan-2-ol + NADP+
butanone + NADPH + H+
butanal + NADPH + H+
1-butanol + NADP+
butanal + NADPH + H+
butan-1-ol + NADP+
butanal + NADPH + H+
butanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 73% relative activity with 0.1 mM or 120% relative activity with 1 mM substrate
-
-
r
butanaldehyde + NADPH
butanol + NADP+
-
-
-
-
r
butane 2,3-dione + NADPH
? + NADP+
-
-
-
-
r
butane-1,2-diol + NADP+
butanal-2-ol + NADPH
-
2% activity compared to cinnamyl alcohol
-
-
r
butane-1,3-diol + NADP+
butanal-3-ol + NADPH
-
5% activity compared to cinnamyl alcohol
-
-
r
butane-1,4-diol + NADP+
butanal-4-ol + NADPH
-
8% activity compared to cinnamyl alcohol
-
-
r
butanol + NADP+
butanal + NADPH
butanol + NADP+
butanal + NADPH + H+
butanone + NADPH + H+
butan-2-ol + NADP+
-
-
-
-
r
butyraldehyde + NADPH + H+
1-butanol + NADP+
112.4% of the activity compared to the substrate 2-butanone
-
-
r
butyraldehyde + NADPH + H+
? + NADP+
-
-
-
-
?
cellobiose + NADP+
? + NADPH + H+
chloral hydrate + NADPH
? + NADP+
-
-
-
-
?
cinnamaldehyde + NADPH
cinnamic alcohol + NADP+
5% of activity with acetaldehyde
-
-
r
cinnamaldehyde + NADPH
cinnamyl alcohol + NADP+
-
-
-
-
r
cinnamaldehyde + NADPH + H+
cinnamyl alcohol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 61% relative activity with 0.1 mM or 7% relative activity with 1 mM substrate
-
-
r
cinnamic aldehyde + NADPH + H+
? + NADP+
-
-
-
-
?
cinnamyl alcohol + NADP+
cinnamaldehyde + NADPH
-
-
-
-
r
cinnamyl alcohol + NADP+
cinnamaldehyde + NADPH + H+
cinnamyl alcohol + NADP+
cinnamylaldehyde + NADPH
-
-
-
-
r
cis-11-hexadecenal + NADPH
cis-11-hexadecenol + NADP+
-
87% relative activity
-
-
ir
coniferaldehyde + NADPH
coniferyl alcohol + NADP+
coniferyl alcohol + NADP+
coniferaldehyde + NADPH + H+
-
7% activity compared to cinnamyl alcohol
-
-
r
coniferyl alcohol + NADP+
coniferylaldehyde + NADPH + H+
-
27% of activity with cinnamyl alcohol
-
-
r
cyclohexancarbaldehye + NADPH
cyclohexanol + NADP+
3% of activity with acetaldehyde
-
-
r
cyclohexanol + NADP+
cyclohexanal + NADPH
-
-
-
-
r
cyclohexanol + NADP+
cyclohexancarbaldehyde + NADPH
14% of activity with ethanol
-
-
r
cyclohexanol + NADP+
cyclohexanone + NADPH + H+
-
about 15% of the activity with butan-2-ol
-
-
?
cyclohexanone + NADPH
cyclohexanol + NADP+
cyclopentanol + NADP+
cyclopentanal + NADPH
-
-
-
-
r
D-arabinose + NADP+
D-arabitol + NADPH + H+
-
200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose
-
-
?
D-arabinose + NADPH
? + NADP+
D-arabitol + NADP+
? + NADPH
-
-
-
-
r
D-erythrose + NADPH
? + NADP+
-
-
-
-
?
D-fructose + NADPH
? + NADP+
-
-
-
-
?
D-galactose + NADPH
? + NADP+
D-galacturonate + NADPH
? + NADP+
-
-
-
-
?
D-glucose + NADP+
? + NADPH + H+
D-glucose + NADPH
? + NADP+
D-glucose + NADPH + H+
sorbitol + NADP+
D-glucuronate + NADPH
L-gulonate + NADP+
D-glucuronate + NADPH + H+
?
D-glucuronate + NADPH + H+
? + NADP+
D-glucuronolactone + NADPH
? + NADP+
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
D-lactaldehyde + NADPH + H+
propane-1,2-diol + NADP+
D-ribose + NADPH
? + NADP+
D-sorbitol + NADP+
? + NADPH
-
-
-
-
r
D-xylose + NADP+
? + NADPH + H+
-
246% of the activity with 2-propanol
-
-
?
D-xylose + NADPH
? + NADP+
daunorubicin + NADPH
? + NADP+
decanal + NADPH
decanol + NADP+
-
no detectable catalysis of the reverse reaction, the oxidation of decanol by NADP+. 100% relative activity
-
-
ir
decanal + NADPH + H+
decanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 21% relative activity with 0.1 mM or 22% relative activity with 1 mM substrate
-
-
r
decanol + NADP+
decanal + NADPH + H+
dehydro-L-ascorbate + NADPH
? + NADP+
-
-
-
-
?
diacetyl + NADPH
? + NADP+
diacetyl + NADPH + H+
acetoin + NADP+
the enzyme can not oxidize acetoin to diacetyl
-
-
ir
dimethylglyoxal + NADPH + H+
? + NADP+
-
270% of the activity with pyruvaldehyde
-
-
?
DL-glyceraldehyde + NADPH + H+
? + NADP+
DL-glyceraldehyde + NADPH + H+
DL-glycerol + NADP+
-
-
-
-
?
DL-glyceraldehyde + NADPH + H+
glycerol + NADP+
dodecanal + NADPH
dodecanol + NADP+
-
55% relative activity
-
-
ir
erythritol + NADP+
? + NADPH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
ethanol + NADP+
acetaldehyde + NADPH + H+
ethyl 4-bromo-3-oxobutanoate + NADPH
(R)-4-bromo-3-hydroxybutanoic acid ethyl ester + NADP+
-
-
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
(R)-4-chloro-3-hydroxybutanoic acid ethyl ester + NADP+
-
-
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
ethyl-4-chloro-3-oxobutanoate + NADPH + H+
ethyl-4-chloro-3-hydroxybutanoate + NADP+
ethylacetoacetate + NADPH
ethyl L-(+)-3-hydroxybutyrate + NADP+
ethylene glycol + NADP+
? + NADPH
-
-
-
-
?
formaldehyde + NADPH
methanol + NADP+
formaldehyde + NADPH + H+
methanol + NADP+
furaldehyde + NADPH
furfuryl alcohol + NADP+
-
-
-
-
?
furan-2-carbaldehyde + NADPH + H+
? + NADP+
-
-
-
-
?
furfural + NADH + H+
furfuryl alcohol + NAD+
furfural + NADPH
furfuryl alcohol + NADP+
furfural + NADPH + H+
furfuryl alcohol + NADP+
glucuronate + NADPH + H+
?
glutaraldehyde + NADPH
? + NADP+
5% of activity with acetaldehyde
-
-
r
glycerol + NADP+
DL-glyceraldehyde + NADPH
glycolaldehyde + NADPH
ethane-1,2-diol + NADP+
heptanal + NADPH
1-heptanol + NADP+
heptanal + NADPH + H+
heptanol + NADP+
-
-
-
-
?
heptanaldehyde + NADPH + H+
? + NADP+
-
-
-
-
?
hexadecanol + NADP+
hexadecanal + NADPH
-
-
-
-
r
hexan-1-ol + NADP+
hexanal + NADPH + H+
hexanal + NADPH + H+
1-hexanol + NADP+
hexanal + NADPH + H+
hexanol + NADP+
hexanal + NADPH + H+
n-hexanol + NADP+
-
-
-
-
?
hexanaldehyde + NADPH + H+
? + NADP+
-
-
-
-
?
hexanol + NADP+
hexanal + NADPH
hexanol + NADP+
hexanal + NADPH + H+
hexyl alcohol + NADP+
?
the native enzyme shows 137.8% activity with hexyl alcohol compared to ethanol
-
-
?
hexyl alcohol + NADP+
hexanal + NADPH + H+
-
-
-
-
?
hydroxyindol-3-ylacetaldehyde + NADPH
? + NADP+
-
-
-
-
r
hydroxypyruvate + ?
?
-
-
-
-
r
indol-3-ylacetaldehyde + NADPH
indol-3-ethanol + NADP+
indolacetaldehyde + NADPH
? + NADP+
-
-
-
-
?
iodoacetaldehyde + NADPH
iodoethan-2-ol + NADP+
-
-
-
-
r
iodohexadecanal + NADPH
iodohexadecanol + NADP+
-
-
-
-
r
isoamyl alcohol + NADP+
3-methylbutanol + NADPH
18% of activity with ethanol
-
-
r
isobutanol + NADP+
isobutanal + NADPH + H+
isobutanol + NADP+
isobutanol + NADPH + H+
-
93% of the activity with ethanol
-
-
r
isobutanol + NADP+
isobutyl aldehyde + NADPH + H+
-
isoform AdhA, 221% of the activity with acetaldehyde
-
-
r
isobutyl aldehyde + NADPH + H+
isobutanol + NADP+
-
isoform AdhA, 469%, isoform AdhB, 48% of the activity with acetaldehyde
-
-
r
isopentanol + NADP+
3-methylbutanal + NADPH + H+
9% of activity with ethanol
-
-
r
isopropanol + NADP+
acetone + NADPH
2% of activity with ethanol
-
-
r
L-arabitol + NADP+
? + NADPH
-
-
-
-
r
L-glyceraldehyde + NADPH
glycerol + NADP+
L-gulonic acid + NADP+
D-glucuronic acid + NADPH
L-gulonic acid + NADPH
D-glucuronic acid + NADP+
L-sorbose + NADPH
? + NADP+
-
-
-
-
r
L-xylose + NADPH
? + NADP+
lauryl aldehyde + NADPH
lauryl alcohol + NADP+
m-chlorobenzaldehyde + NADPH + H+
m-chlorobenzyl alcohol + NADP+
-
-
-
-
r
m-fluorobenzaldehyde + NADPH + H+
m-fluorobenzyl alcohol + NADP+
m-methoxybenzaldehyde + NADPH + H+
m-methoxybenzyl alcohol + NADP+
m-methylbenzaldehyde + NADPH
m-methylbenzyl alcohol + NADP+
m-nitrobenzaldehyde + NADPH
m-nitrobenzylalcohol + NADP+
malondialdehyde + NADPH
?
-
-
-
-
r
menadione + NADPH
? + NADP+
-
G2
-
-
?
meso-2,3-butanediol + NADP+
(S)-2-acetoin + NADPH + H+
the enzyme shows higher oxidation activities on the (2R,3R)-(-)-2,3-butanediol and meso-2,3-butanediol than on the (2S,3S)-(+)-2,3-butanediol, indicating that the enzyme predominantly oxidizes the R-hydroxyl group of 2,3-butanediol and minorly functions on the S-hydroxyl group. When meso-2,3-butanediol is oxidized, (S)-2-acetoin is the predominant product, with an enantiomeric excess (ee) of 88%, while oxidation of meso-2,3-butanediol results in the production of (R)-2-acetoin with higher stereoselectivity (94% ee)
-
-
r
methanol + NADP+
formaldehyde + NADPH + H+
-
-
-
-
r
methyl 4-bromo-3-oxobutanoate + NADPH + H+
methyl (S)-4-bromo-3-hydroxybutanoate + NADP+
methyl acetoacetate + NADH + H+
methyl (R)-3-hydroxy-butanoate + NAD+
-
-
-
-
?
methyl acetoacetate + NADPH + H+
methyl (R)-3-hydroxy-butanoate + NADP+
-
-
-
-
?
methylglyoxal + NADPH
? + NADP+
mevaldate + NADPH
? + NADP+
-
-
-
-
?
n-butanol + NADP+
butyraldehyde + NADPH
n-butyraldehyde + NADPH
n-butanol + NADP+
n-decanal + NADPH + H+
n-decanol + NADP+
n-dodecanal + NADPH + H+
n-dodecanol + NADP+
n-heptanal + NADPH
heptanol + NADP+
n-heptanol + NADP+
n-heptanal + NADPH
n-hexadecanal + NADPH + H+
n-hexadecanol + NADP+
n-hexanol + NADP+
n-hexanal + NADPH
n-octadecanal + NADPH + H+
n-octadecanol + NADP+
-
-
-
-
r
n-octanal + NADPH + H+
n-octanol + NADP+
n-propanol + NADP+
propionaldehyde + NADPH
n-tetradecanal + NADPH + H+
n-tetradecanol + NADP+
-
-
-
-
r
nonanal + NADPH
? + NADP+
-
-
-
-
?
o-fluorobenzaldehyde + NADPH + H+
o-fluorobenzyl alcohol + NADP+
o-methylbenzaldehyde + NADPH
o-methylbenzyl alcohol + NADP+
o-nitrobenzaldehyde + NADPH
o-nitrobenzylalcohol + NADP+
octan-1-ol + NADP+
octanal + NADPH + H+
octanal + NADPH + H+
1-octanol + NADP+
octanal + NADPH + H+
octanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 42% relative activity with 0.1 mM or 80% relative activity with 1 mM substrate
-
-
r
octanaldehyde + NADPH + H+
? + NADP+
-
-
-
-
?
octanol + NADP+
octanal + NADPH
-
52% of activity with cinnamyl alcohol
-
-
r
octanol + NADP+
octanal + NADPH + H+
octyl 4-chloro-3-oxobutanoate + NADPH
(R)-4-chloro-3-hydroxybutanoic acid ethyl ester + NADP+
-
-
-
-
?
p-carboxybenzaldehyde + NADPH
p-carboxybenzyl alcohol + NADP+
p-chlorobenzaldehyde + NADPH + H+
p-chlorobenzyl alcohol + NADP+
p-cyanobenzaldehyde + NADPH
p-cyanobenzyl alcohol + NADP+
p-fluorobenzaldehyde + NADPH + H+
p-fluorobenzyl alcohol + NADP+
p-hydroxyphenylacetaldehyde + NADPH + H+
p-hydroxyphenylethanol + NADP+
p-hydroxyphenylglycolaldehyde + NADPH
4-hydroxyphenylethylalcohol + NADP+
p-methoxybenzaldehyde + NADPH + H+
p-methoxybenzyl alcohol + NADP+
p-methylbenzaldehyde + NADPH
p-methylbenzyl alcohol + NADP+
p-nitroacetophenone + NADPH
? + NADP+
-
-
-
-
r
p-nitrobenzaldehyde + NADPH
p-nitrobenzylalcohol + NADP+
p-tolualdehyde + NADPH
toluol + NADP+
-
-
-
-
?
pent-1-en-3-ol + NADP+
pent-1-en-3-one + NADPH + H+
-
good substrate
-
-
?
pentan-1-ol + NADP+
pentanal + NADPH + H+
pentanal + NADPH
1-pentanol + NADP+
pentanal + NADPH
pentanol + NADP+
pentanal + NADPH + H+
pentan-1-ol + NADP+
pentanal + NADPH + H+
pentanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 100% relative activity with 0.1 mM or 107% relative activity with 1 mM substrate
-
-
r
pentanol + NADP+
pentanal + NADPH
pentanol + NADP+
pentanal + NADPH + H+
phenylacetaldehyde + NADPH + H+
2-phenylethanol + NADP+
phenylacetaldehyde + NADPH + H+
phenylethanol + NADP+
phenylacetone + NADPH + H+
(S)-1-phenyl-2-propanol + NADP+
-
-
-
-
r
phenylethanol + NADP+
? + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 15% relative activity with 1 mM or 24% relative activity with 10 mM substrate
-
-
?
phenylethanol + NADP+
phenylacetaldehyde + NADPH + H+
-
15% activity with 1 mM phenylethanol, 24% activity with 10 mM phenylethanol
-
-
r
phenylethylamine + ?
?
-
-
-
-
r
phenylglyoxal + NADPH
? + NADP+
propan-1-ol + NADP+
propanal + NADPH + H+
propan-2-ol + NADP+
acetone + NADPH + H+
-
good substrate
-
-
?
propan-2-ol + NADP+
propanone + NADPH
propanal + NADPH + H+
1-propanol + NADP+
propanal + NADPH + H+
propan-1-ol + NADP+
-
isoform AdhA, 50%, isoform AdhB, 62% of the activity with acetaldehyde
-
-
r
propanal + NADPH + H+
propanol + NADP+
propanaldehyde + NADPH
propanol + NADP+
-
-
-
-
r
propane-1,2-diol + NADP+
lactaldehyde + NADPH
propanol + NADP+
propanal + NADPH
propanol + NADP+
propanal + NADPH + H+
-
21% activity with 1 mM propanol, 84% activity with 10 mM propanol
26% activity with 0.1 mM propanal, 87% activity with 1 mM propanal
-
r
propionaldehyde + NADPH
propanol + NADP+
propionaldehyde + NADPH + H+
propanol + NADP+
-
-
-
-
?
propiophenone + NADPH
? + NADP+
-
-
-
-
?
pyridoxal + NADPH
4,5-bis(hydroxymethyl)-2-methylpyridin-3-ol + NADP+
-
-
-
-
?
pyruvaldehyde + NADP+
? + NADPH + H+
-
-
-
-
r
pyruvaldehyde + NADPH
? + NADP+
-
-
-
-
?
pyruvaldehyde + NADPH + H+
lactaldehyde + NADP+
pyruvate + NADPH
2-hydroxypropanoic acid + NADP+
-
-
-
-
?
salicylaldehyde + NADPH
2-hydroxymethylphenol + NADP+
-
-
-
-
?
salicylaldehyde + NADPH
salicylalcohol + NADP+
1% of activity with acetaldehyde
-
-
r
salicylaldehyde + NADPH
salicylic alcohol + NADP+
-
-
-
-
?
sinapaldehyde + NADPH + H+
sinapyl alcohol + NADP+
succinic semialdehyde + NADPH + H+
4-hydroxybutyrate + NADP+
tetradecanal + NADPH
tetradecanol
-
-
-
-
r
trans-2-decenal + NADPH
trans-2-decen-1-ol + NADP+
-
-
-
-
?
trans-2-hexenal + NADPH
trans-2-hexen-1-ol + NADP+
-
-
-
-
?
trans-2-hexenol + NADP+
trans-2-hexenal + NADPH
trans-2-nonenal + NADPH
trans-2-nonen-1-ol + NADP+
-
33% activity compared to cinnamaldehyde
-
-
r
trans-2-nonenal + NADPH
trans-2-nonenol + NADP+
-
39% of activity with cinnamaldehyde
-
-
r
trans-2-nonenaldehyde + NADPH + H+
? + NADP+
-
-
-
-
?
trans-cinnamaldehyde + NADPH
cinnamyl alcohol + NADP+
tridecanal + NADPH
tridecanol + NADP+
-
-
-
-
r
Tris + NADP+
? + NADPH
-
-
-
-
?
undecanal + NADPH
undecanol + NADP+
-
-
-
-
?
valeraldehyde + NADPH + H+
? + NADP+
-
-
-
-
?
vanillin + NADPH
4-hydroxy-3-methoxybenzyl alcohol + NADP+
veratraldehyde + NADPH
3,4-dimethoxybenzyl alcohol + NADP+
vitamin A aldehyde + NADPH
? + NADP+
-
-
-
-
?
additional information
?
-
(+)-camphorquinone + NADPH

? + NADP+
-
-
-
-
?
(+)-camphorquinone + NADPH
? + NADP+
-
-
-
-
?
1,3-propanediol + NADP+

? + NADPH
-
-
-
-
?
1,3-propanediol + NADP+
? + NADPH
-
primary alcohol dehydrogenase
-
-
?
1,3-propanediol + NADP+
? + NADPH
-
-
-
-
?
1,5-pentanediol + NADP+

? + NADPH + H+
-
-
-
-
1,5-pentanediol + NADP+
? + NADPH + H+
-
-
-
-
1-butanol + NADP+

butanal + NADPH
-
25% of activity with 1-hexanol
-
-
r
1-butanol + NADP+
butanal + NADPH
-
42% activity compared to cinnamyl alcohol
-
-
r
1-butanol + NADP+

butanal + NADPH + H+
-
-
-
-
?
1-butanol + NADP+
butanal + NADPH + H+
-
169% of the activity compared to ethanol
-
-
r
1-butanol + NADP+
butanal + NADPH + H+
-
130% of the activity with ethanol
-
-
r
1-butanol + NADP+
butanal + NADPH + H+
-
130% of the activity with ethanol
-
-
r
1-butanol + NADP+
butanal + NADPH + H+
the native enzyme shows 154.6% activity with 1-butanol compared to ethanol
-
-
?
1-butanol + NADP+
butanal + NADPH + H+
the native enzyme shows 154.6% activity with 1-butanol compared to ethanol
-
-
?
1-butanol + NADP+

butyraldehyde + NADPH + H+
-
-
-
-
r
1-butanol + NADP+
butyraldehyde + NADPH + H+
5.1% of the activity compared to the substrate 2-butanol
-
-
r
1-heptanol + NADP+

heptanal + NADPH + H+
-
-
-
-
?
1-heptanol + NADP+
heptanal + NADPH + H+
-
86% of the activity compared to ethanol
-
-
r
1-heptanol + NADP+
heptanal + NADPH + H+
-
114% of the activity with ethanol
-
-
r
1-heptanol + NADP+
heptanal + NADPH + H+
-
114% of the activity with ethanol
-
-
r
1-heptanol + NADP+
heptanal + NADPH + H+
the native enzyme shows 94% activity with 1-heptanol compared to ethanol
-
-
?
1-heptanol + NADP+
heptanal + NADPH + H+
the native enzyme shows 94% activity with 1-heptanol compared to ethanol
-
-
?
1-hexanol + NADP+

hexanal + NADPH
-
-
-
-
r
1-hexanol + NADP+
hexanal + NADPH
-
45% activity compared to cinnamyl alcohol
-
-
r
1-hexanol + NADP+

hexanal + NADPH + H+
-
95% of the activity compared to ethanol
-
-
r
1-hexanol + NADP+
hexanal + NADPH + H+
-
165% of the activity with ethanol
-
-
r
1-octanol + NADP+

octanal + NADPH
-
31% of activity with 1-hexanol
-
-
r
1-octanol + NADP+
octanal + NADPH
-
-
-
-
r
1-octanol + NADP+
octanal + NADPH
-
30% activity compared to cinnamyl alcohol
-
-
r
1-octanol + NADP+
octanal + NADPH
-
-
-
-
r
1-octanol + NADP+
octanal + NADPH
-
-
-
-
r
1-octanol + NADP+

octanal + NADPH + H+
-
-
-
-
?
1-octanol + NADP+
octanal + NADPH + H+
-
57% of the activity compared to ethanol
-
-
r
1-octanol + NADP+
octanal + NADPH + H+
the native enzyme shows 68.8% activity with 1-octanol compared to ethanol
-
-
?
1-octanol + NADP+
octanal + NADPH + H+
the native enzyme shows 68.8% activity with 1-octanol compared to ethanol
-
-
?
1-pentanol + NADP+

n-pentanal + NADPH
-
-
-
-
r
1-pentanol + NADP+
n-pentanal + NADPH
-
-
-
-
r
1-pentanol + NADP+
n-pentanal + NADPH
-
-
-
-
r
1-pentanol + NADP+
n-pentanal + NADPH
-
primary alcohol dehydrogenase
-
-
r
1-pentanol + NADP+
n-pentanal + NADPH
-
-
-
-
r
1-pentanol + NADP+
n-pentanal + NADPH
-
-
-
-
r
1-pentanol + NADP+
n-pentanal + NADPH
-
-
-
-
r
1-pentanol + NADP+

pentanal + NADPH
-
58% of activity with 1-hexanol
-
-
r
1-pentanol + NADP+
pentanal + NADPH
-
46% activity compared to cinnamyl alcohol
-
-
r
1-pentanol + NADP+

pentanal + NADPH + H+
-
172% of the activity compared to ethanol
-
-
r
1-pentanol + NADP+
pentanal + NADPH + H+
-
135% of the activity with ethanol
-
-
r
1-pentanol + NADP+
pentanal + NADPH + H+
the native enzyme shows 184% activity with 1-pentanol compared to ethanol
-
-
?
1-pentanol + NADP+
pentanal + NADPH + H+
the native enzyme shows 184% activity with 1-pentanol compared to ethanol
-
-
?
1-propanol + NADP+

propanal + NADPH + H+
-
156% of the activity compared to ethanol
-
-
r
1-propanol + NADP+
propanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 21% relative activity with 1 mM or 84% relative activity with 10 mM substrate
-
-
r
1-propanol + NADP+
propanal + NADPH + H+
15.1% of the activity compared to the substrate 2-butanol
-
-
?
1-propanol + NADP+
propanal + NADPH + H+
-
102% of the activity with ethanol
-
-
r
1-propanol + NADP+
propanal + NADPH + H+
-
-
-
-
?
1-propanol + NADP+
propanal + NADPH + H+
-
-
-
?
1-propanol + NADP+
propanal + NADPH + H+
the native enzyme shows 120.6% activity with 1-propanol compared to ethanol
-
-
?
2,3-pentanedione + NADPH + H+

? + NADP+
-
-
-
-
?
2,3-pentanedione + NADPH + H+
? + NADP+
-
secondary alcohol dehydrogenase
-
-
?
2-butanol + NADP+

?
-
-
-
?
2-butanol + NADP+
?
-
-
-
?
2-butanol + NADP+

butan-2-one + NADPH
-
-
-
-
r
2-butanol + NADP+
butan-2-one + NADPH
-
-
-
-
r
2-butanol + NADP+
butan-2-one + NADPH
-
-
-
-
r
2-butanol + NADP+
butan-2-one + NADPH
-
2° Adh
-
-
r
2-methoxybenzaldehyde + NADPH + H+

2-methoxybenzyl alcohol + NADP+
-
-
-
-
?
2-methoxybenzaldehyde + NADPH + H+
2-methoxybenzyl alcohol + NADP+
-
-
-
-
?
2-methyl-1-propanol + NADP+

2-methylpropanal + NADPH
-
28% activity compared to cinnamyl alcohol
-
-
r
2-methyl-1-propanol + NADP+
2-methylpropanal + NADPH
-
-
-
-
?
2-methylbutanal + NADPH

2-methyl-1-butanol + NADP+
-
89% activity compared to cinnamaldehyde
-
-
r
2-methylbutanal + NADPH
2-methyl-1-butanol + NADP+
-
92% of activity with cinnamaldehyde
-
-
r
2-methylpropanal + NADPH

2-methyl-1-propanol + NADP+
-
63% activity compared to cinnamaldehyde
-
-
r
2-methylpropanal + NADPH
2-methyl-1-propanol + NADP+
-
67% of activity with cinnamaldehyde
-
-
r
2-pentanol + NADP+

2-pentanone + NADPH + H+
-
-
-
-
?
2-pentanol + NADP+
2-pentanone + NADPH + H+
maximal activity in oxidative reaction
-
-
r
2-pentanol + NADP+
2-pentanone + NADPH + H+
66.3% of the activity compared to the substrate 2-butanol
-
-
r
2-phenylethanol + NADP+

2-phenylacetaldehyde + NADPH + H+
-
70% of the activity with ethanol
-
-
r
2-phenylethanol + NADP+
2-phenylacetaldehyde + NADPH + H+
the native enzyme shows 40.2% activity with 2-phenylethanol compared to ethanol
-
-
?
2-phenylethanol + NADP+

phenylacetaldehyde + NADPH + H+
-
-
-
-
?
2-phenylethanol + NADP+
phenylacetaldehyde + NADPH + H+
-
76% of the activity compared to ethanol
-
-
r
2-propanol + NADP+

acetone + NADPH
-
-
-
-
r
2-propanol + NADP+
acetone + NADPH
-
-
-
-
r
2-propanol + NADP+

acetone + NADPH + H+
-
-
-
?
2-propanol + NADP+
acetone + NADPH + H+
-
-
-
-
r
2-propanol + NADP+
acetone + NADPH + H+
88% of the activity compared to the substrate 2-butanol
-
-
r
2-propanol + NADP+
acetone + NADPH + H+
-
-
-
-
?
2-propanol + NADP+
acetone + NADPH + H+
-
-
-
-
?
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+

1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone
-
-
r
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+
1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone
-
-
r
3,4-dihydroxyphenylglycolaldehyde + NADPH

? + NADP+
-
-
-
-
r
3,4-dihydroxyphenylglycolaldehyde + NADPH
? + NADP+
-
low Km reductase
-
-
r
3-methyl-1,5-pentanediol + NADP+

?
-
-
-
?
3-methyl-1,5-pentanediol + NADP+
?
-
-
-
?
3-pyridinecarboxaldehyde + NADPH

3-pyridinemethanol + NADP+
-
-
-
-
-
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
AR3
-
-
-
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
-
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
ir
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
286165, 286167, 286172, 286174, 286176, 286177, 286178, 286179, 286180, 286181, 286182, 286185 -
-
r
4-methoxybenzaldehyde + NADPH + H+

4-methoxybenzyl alcohol + NADP+
-
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
-
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
-
-
-
-
?
4-pyridinecarboxaldehyde + NADPH

4-pyridinemethanol + NADP+
-
-
-
-
-
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
r
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
r
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
r
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
r
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
r
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
?
5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+

(furan-2,5-diyl)dimethanol + NADP+
-
-
-
-
?
5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+
(furan-2,5-diyl)dimethanol + NADP+
-
-
-
-
?
5-hydroxymethylfurfural + NADPH + H+

5-hydroxymethylfurfuryl alcohol + NADP+
-
-
-
?
5-hydroxymethylfurfural + NADPH + H+
5-hydroxymethylfurfuryl alcohol + NADP+
-
-
-
?
acetaldehyde + NADPH + H+

ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 1% relative activity with 0.1 mM or 23% relative activity with 1 mM substrate
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
anisaldehyde + NADPH

4-methoxybenzyl alcohol + NADP+
-
72% of activity with cinnamaldehyde
-
-
r
anisaldehyde + NADPH
4-methoxybenzyl alcohol + NADP+
-
83% activity compared to cinnamaldehyde
-
-
r
anisaldehyde + NADPH + H+

anisyl alcohol + NADP+
-
-
-
-
r
anisaldehyde + NADPH + H+
anisyl alcohol + NADP+
-
-
-
-
r
anisaldehyde + NADPH + H+
anisyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH

benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
-
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
8% of activity with acetaldehyde
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
45% activity compared to cinnamaldehyde
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
72% of activity with cinnamaldehyde
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
relative activity 4%
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
?
benzyl alcohol + NADP+

benzaldehyde + NADPH
-
45% of activity with cinnamyl alcohol
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH
-
78% activity compared to cinnamyl alcohol
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH
-
-
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH
-
-
-
-
r
benzyl alcohol + NADP+

benzaldehyde + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 42% relative activity with 1 mM or 79% relative activity with 10 mM substrate
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
42% activity with 1 mM benzyl alcohol, 79% activity with 10 mM benzyl alcohol
30% activity with 0.1 mM benzaldehyde, 14% activity with 1 mM benzaldehyde
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
180% of the activity with 2-propanol
-
-
?
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
180% of the activity with 2-propanol
-
-
?
butan-1-ol + NADP+

butanal + NADPH + H+
-
isoform ADH-I, 94%, isoform ADH II, 32% of the actvity with hexan-1-ol
-
-
?
butan-1-ol + NADP+
butanal + NADPH + H+
-
isoform ADH-I, 94%, isoform ADH II, 32% of the actvity with hexan-1-ol
-
-
?
butan-1-ol + NADP+
butanal + NADPH + H+
-
isoform AdhA, 238% of the activity with acetaldehyde
-
-
r
butan-1-ol + NADP+
butanal + NADPH + H+
-
-
-
?
butan-1-ol + NADP+
butanal + NADPH + H+
-
-
-
?
butan-2-ol + NADP+

butanone + NADPH + H+
-
isoform ADH-I, 89%, isoform ADH II, 23% of the actvity with hexan-1-ol
-
-
?
butan-2-ol + NADP+
butanone + NADPH + H+
-
isoform ADH-I, 89%, isoform ADH II, 23% of the actvity with hexan-1-ol
-
-
?
butan-2-ol + NADP+
butanone + NADPH + H+
-
good substrate
-
-
r
butanal + NADPH + H+

1-butanol + NADP+
47% of activity with acetaldehyde
-
-
r
butanal + NADPH + H+
1-butanol + NADP+
-
19% activity compared to cinnamaldehyde
-
-
r
butanal + NADPH + H+
1-butanol + NADP+
-
57% of activity with cinnamaldehyde
-
-
r
butanal + NADPH + H+

butan-1-ol + NADP+
-
isoform AdhA, 625%, isoform AdhB, 108% of the activity with acetaldehyde
-
-
r
butanal + NADPH + H+
butan-1-ol + NADP+
-
-
-
?
butanal + NADPH + H+
butan-1-ol + NADP+
-
-
-
?
butanal + NADPH + H+
butan-1-ol + NADP+
-
-
-
-
?
butanol + NADP+

butanal + NADPH
68% of activity with ethanol
-
-
r
butanol + NADP+
butanal + NADPH
-
24% of activity with cinnamyl alcohol
-
-
r
butanol + NADP+

butanal + NADPH + H+
-
23% activity with 1 mM butanol, 70% activity with 10 mM butanol
73% activity with 0.1 mM butanal, 120% activity with 1 mM butanal
-
r
butanol + NADP+
butanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 23% relative activity with 1 mM or 70% relative activity with 10 mM substrate
-
-
r
cellobiose + NADP+

? + NADPH + H+
-
71% of the activity with 2-propanol
-
-
?
cellobiose + NADP+
? + NADPH + H+
-
71% of the activity with 2-propanol
-
-
?
cinnamyl alcohol + NADP+

cinnamaldehyde + NADPH + H+
-
100% activity with 1 mM cinnamyl alcohol, 116% activity with 10 mM hexanol
61% activity with 0.1 mM cinnamaldehyde, 7% activity with 1 mM cinnamaldehyde
-
r
cinnamyl alcohol + NADP+
cinnamaldehyde + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 100% relative activity with 1 mM or 116% relative activity with 10 mM substrate
-
-
r
coniferaldehyde + NADPH

coniferyl alcohol + NADP+
-
10% of activity with cinnamaldehyde
-
-
r
coniferaldehyde + NADPH
coniferyl alcohol + NADP+
-
15% activity compared to cinnamaldehyde
-
-
r
coniferaldehyde + NADPH
coniferyl alcohol + NADP+
-
converted less efficiently than eutypine
-
-
?
cyclohexanone + NADPH

cyclohexanol + NADP+
-
-
-
-
?
cyclohexanone + NADPH
cyclohexanol + NADP+
-
G2
-
-
?
cyclohexanone + NADPH
cyclohexanol + NADP+
-
-
-
-
?
D-arabinose + NADPH

? + NADP+
-
-
-
-
?
D-arabinose + NADPH
? + NADP+
-
-
-
-
r
D-galactose + NADPH

? + NADP+
-
-
-
-
-
D-galactose + NADPH
? + NADP+
-
-
-
-
?
D-glucose + NADP+

? + NADPH + H+
-
146% of the activity with 2-propanol
-
-
?
D-glucose + NADP+
? + NADPH + H+
-
146% of the activity with 2-propanol
-
-
?
D-glucose + NADPH

? + NADP+
-
placenta aldehyde reductase I
-
-
?
D-glucose + NADPH
? + NADP+
-
-
-
-
?
D-glucose + NADPH + H+

sorbitol + NADP+
-
-
-
-
?
D-glucose + NADPH + H+
sorbitol + NADP+
-
-
-
-
?
D-glucuronate + NADPH

?
0.1 M phosphat buffer, pH 7.0
-
-
?
D-glucuronate + NADPH
?
0.1 M phosphat buffer, pH 7.0
-
-
?
D-glucuronate + NADPH

L-gulonate + NADP+
-
-
-
-
-
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
AR1
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?, r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?, r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
aldehyde reductase II
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?, r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
286165, 286172, 286174, 286175, 286176, 286177, 286182, 286184, 286190, 286192, 286196 -
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?, r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
-
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
286165, 286167, 286172, 286174, 286176, 286177, 286178, 286179, 286182, 286185, 286196 -
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH + H+

?
0.1 M phosphate buffer, pH 7.0
-
-
?
D-glucuronate + NADPH + H+
?
0.1 M phosphate buffer, pH 7.0
-
-
?
D-glucuronate + NADPH + H+

? + NADP+
-
-
-
r
D-glucuronate + NADPH + H+
? + NADP+
-
-
-
r
D-glucuronate + NADPH + H+
? + NADP+
-
-
-
r
D-glucuronate + NADPH + H+
? + NADP+
-
-
-
r
D-glucuronolactone + NADPH

? + NADP+
-
-
-
-
?
D-glucuronolactone + NADPH
? + NADP+
-
-
-
-
?
D-glucuronolactone + NADPH
? + NADP+
-
-
-
-
?
D-glyceraldehyde + NADPH + H+

glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
286149, 286168, 286175, 286176, 286177, 286181, 286182, 286184, 286186, 286187, 286196, 286199 -
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
286165, 286172, 286174, 286175, 286176, 286177, 286181, 286182, 286184, 286192, 286196, 286204 -
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
ir
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
ir
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
286165, 286167, 286168, 286171, 286172, 286174, 286176, 286177, 286178, 286179, 286181, 286182, 286196 -
-
r
D-lactaldehyde + NADPH + H+

propane-1,2-diol + NADP+
-
-
-
-
r
D-lactaldehyde + NADPH + H+
propane-1,2-diol + NADP+
-
-
-
-
r
D-lactaldehyde + NADPH + H+
propane-1,2-diol + NADP+
-
-
-
-
r
D-ribose + NADPH

? + NADP+
-
-
-
-
?
D-ribose + NADPH
? + NADP+
-
-
-
-
?
D-xylose + NADPH

? + NADP+
-
-
-
-
?
D-xylose + NADPH
? + NADP+
-
AR3
-
-
?
D-xylose + NADPH
? + NADP+
-
-
-
-
?
D-xylose + NADPH
? + NADP+
-
-
-
-
-
D-xylose + NADPH
? + NADP+
-
-
-
-
-
D-xylose + NADPH
? + NADP+
-
-
-
-
-
D-xylose + NADPH
? + NADP+
-
-
-
-
?
D-xylose + NADPH
? + NADP+
-
-
-
-
?
daunorubicin + NADPH

? + NADP+
-
-
-
-
?
daunorubicin + NADPH
? + NADP+
-
-
-
-
?
daunorubicin + NADPH
? + NADP+
-
-
-
-
?
decanol + NADP+

decanal + NADPH + H+
-
3% activity with 1 mM decanol, 6% activity with 10 mM decanol
21% activity with 0.1 mM decanal, 22% activity with 1 mM decanal
-
r
decanol + NADP+
decanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 3% relative activity with 1 mM or 6% relative activity with 10 mM substrate
-
-
r
diacetyl + NADPH

? + NADP+
-
-
-
-
-
diacetyl + NADPH
? + NADP+
-
-
-
-
?
diacetyl + NADPH
? + NADP+
-
-
-
-
?
diacetyl + NADPH
? + NADP+
-
-
-
-
?
DL-glyceraldehyde + NADPH + H+

? + NADP+
-
-
-
-
?
DL-glyceraldehyde + NADPH + H+
? + NADP+
-
-
-
-
?
DL-glyceraldehyde + NADPH + H+

glycerol + NADP+
-
-
-
-
-
DL-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
?
ethanol + NAD+

acetaldehyde + NADH + H+
-
kcat/Km for NAD+ is 1900fold lower compared to NADP+
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
-
-
-
r
ethanol + NADP+

acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
isoform AdhB: no oxidation of ethanol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
kcat/Km for NAD+ is 1900fold lower compared to NADP+
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
-
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
2% activity compared to cinnamyl alcohol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
4% of activity with cinnamyl alcohol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
19% activity with 1 mM ethanol, 91% activity with 10 mM ethanol
1% activity with 0.1 mM acetaldehyde, 23% activity with 1 mM acetaldehyde
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 19% relative activity with 1 mM or 91% relative activity with 10 mM substrate
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
5.7% of the activity compared to the substrate 2-butanol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
the native enzyme shows 100% activity with ethanol
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
the native enzyme shows 100% activity with ethanol
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethyl 4-chloro-3-oxobutanoate + NADPH + H+

ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl-4-chloro-3-oxobutanoate + NADPH + H+

ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl-4-chloro-3-oxobutanoate + NADPH + H+
ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl-4-chloro-3-oxobutanoate + NADPH + H+
ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethylacetoacetate + NADPH

ethyl L-(+)-3-hydroxybutyrate + NADP+
-
-
-
-
?
ethylacetoacetate + NADPH
ethyl L-(+)-3-hydroxybutyrate + NADP+
-
-
-
-
?
formaldehyde + NADPH

methanol + NADP+
6% of activity with acetaldehyde
-
-
ir
formaldehyde + NADPH
methanol + NADP+
-
methanal
-
-
?
formaldehyde + NADPH
methanol + NADP+
-
-
-
-
?
formaldehyde + NADPH
methanol + NADP+
-
-
-
-
?
formaldehyde + NADPH
methanol + NADP+
-
-
-
-
?
formaldehyde + NADPH + H+

methanol + NADP+
-
-
-
?
formaldehyde + NADPH + H+
methanol + NADP+
-
-
-
?
furfural + NADH + H+

furfuryl alcohol + NAD+
-
-
-
?
furfural + NADH + H+
furfuryl alcohol + NAD+
-
-
-
?
furfural + NADPH

furfuryl alcohol + NADP+
-
27% of activity with cinnamaldehyde
-
-
r
furfural + NADPH
furfuryl alcohol + NADP+
-
54% activity compared to cinnamaldehyde
-
-
r
furfural + NADPH + H+

furfuryl alcohol + NADP+
-
-
-
?
furfural + NADPH + H+
furfuryl alcohol + NADP+
-
-
-
?
glucuronate + NADPH

?
-
-
-
?
glucuronate + NADPH
?
-
-
-
?
glucuronate + NADPH + H+

?
-
-
-
?
glucuronate + NADPH + H+
?
-
-
-
?
glycerol + NADP+

DL-glyceraldehyde + NADPH
-
-
-
-
-
glycerol + NADP+
DL-glyceraldehyde + NADPH
-
-
-
-
r
glycerol + NADP+
DL-glyceraldehyde + NADPH
-
-
-
-
r
glycerol + NADP+
DL-glyceraldehyde + NADPH
-
-
-
-
r
glycolaldehyde + NADPH

ethane-1,2-diol + NADP+
-
-
-
-
-
glycolaldehyde + NADPH
ethane-1,2-diol + NADP+
-
-
-
-
r
heptanal + NADPH

1-heptanol + NADP+
-
86% activity compared to cinnamaldehyde
-
-
r
heptanal + NADPH
1-heptanol + NADP+
-
88% of activity with cinnamaldehyde
-
-
r
hexan-1-ol + NADP+

hexanal + NADPH + H+
-
-
-
-
?
hexan-1-ol + NADP+
hexanal + NADPH + H+
-
best substrate tested
-
-
?
hexan-1-ol + NADP+
hexanal + NADPH + H+
-
-
-
-
?
hexanal + NADPH + H+

1-hexanol + NADP+
-
88% activity compared to cinnamaldehyde
-
-
r
hexanal + NADPH + H+
1-hexanol + NADP+
-
89% of activity with cinnamaldehyde
-
-
r
hexanal + NADPH + H+

hexanol + NADP+
-
-
-
-
r
hexanal + NADPH + H+
hexanol + NADP+
-
-
-
-
r
hexanal + NADPH + H+
hexanol + NADP+
-
-
-
-
r
hexanal + NADPH + H+
hexanol + NADP+
-
-
-
-
r
hexanal + NADPH + H+
hexanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 53% relative activity with 0.1 mM or 100% relative activity with 1 mM substrate
-
-
r
hexanal + NADPH + H+
hexanol + NADP+
-
-
-
-
?
hexanol + NADP+

hexanal + NADPH
60% of activity with ethanol
-
-
r
hexanol + NADP+
hexanal + NADPH
-
154% of activity with cinnamyl alcohol
-
-
r
hexanol + NADP+

hexanal + NADPH + H+
-
27% activity with 1 mM hexanol, 40% activity with 10 mM hexanol
53% activity with 0.1 mM hexanal, 100% activity with 1 mM hexanal
-
r
hexanol + NADP+
hexanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 27% relative activity with 1 mM or 40% relative activity with 10 mM substrate
-
-
r
indol-3-ylacetaldehyde + NADPH

indol-3-ethanol + NADP+
-
-
-
-
-
indol-3-ylacetaldehyde + NADPH
indol-3-ethanol + NADP+
-
-
-
-
r
indol-3-ylacetaldehyde + NADPH
indol-3-ethanol + NADP+
-
-
-
-
-
indol-3-ylacetaldehyde + NADPH
indol-3-ethanol + NADP+
-
-
-
-
r
isobutanol + NADP+

isobutanal + NADPH + H+
-
122% of the activity compared to ethanol
-
-
r