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Information on EC 1.1.1.2 - alcohol dehydrogenase (NADP+)
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EC Tree
1.1.1.2 alcohol dehydrogenase (NADP+)IUBMB Comments
A zinc protein. Some members of this group oxidize only primary alcohols; others act also on secondary alcohols. May be identical with EC 1.1.1.19 (L-glucuronate reductase), EC 1.1.1.33 [mevaldate reductase (NADPH)] and EC 1.1.1.55 [lactaldehyde reductase (NADPH)]. Re-specific with respect to NADPH.
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Word Map
- 1.1.1.2
-
polyol
- lens
- horse
- nerve
- neuropathy
- sorbinil
- cataract
- mellitus
- hyperglycemia
- retinopathy
- retinal
- galactose
-
glycation
-
sciatic
- fructose
- nad+
- streptozotocin
-
adhes
- myo-inositol
-
streptozotocin-induced
- n-demethylase
- akr1c3
- aminopyrine
- phenobarbital
- aniline
-
streptozotocin-diabetic
-
benzoapyrene
-
osmolytes
-
diabetes-induced
- pyrazole
-
nadh-cytochrome
-
cataractogenesis
-
drug-metabolizing
- pericytes
-
dihydrodiols
- galactosemia
- benzphetamine
-
mixed-function
- xylitol
- ethylmorphine
-
n-demethylation
- 7-ethoxycoumarin
-
hyperglycemia-induced
-
sural
-
17beta-hydroxysteroids
-
alpha-hydroxysteroid
- synthesis
- o-quinones
-
udp-glucuronyltransferase
- medicine
- 17beta-hsds
- 4-methylpyrazole
- agriculture
- industry
- analysis
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
aldose reductase, nadph-cytochrome c reductase, aldo-keto reductase, liver alcohol dehydrogenase, adh-1, adh-2, short-chain alcohol dehydrogenase, nadph-dependent aldehyde reductase, tsadh319, akr1a4, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2° Adh
3-DG-reducing enzyme
-
-
-
-
AAur_2040
ADH
ADH-1
ADH-2
ADH-I
ADH-II
Adh319
ADH4
Adh6
AdhA
AKR1A1
alcohol dehydrogenase [NADP(+)]
Alcohol dehydrogenase [NADP+]
-
-
-
-
aldehyde reductase
aldehyde reductase (NADPH2)
-
-
-
-
Aldo-keto reductase family 1 member A1
-
-
-
-
aldose reductase
ALR
-
-
-
-
ALR 1
-
-
-
-
ALR1
BdhA
daunorubicin reductase
hexogenate dehydrogenase
high-Km aldehyde reductase
-
-
-
-
L-hexonate dehydrogenase
low-Km aldehyde reductase
-
-
-
-
Mpd1
NADP-alcohol dehydrogenase
-
-
-
-
NADP-aldehyde reductase
-
-
-
-
NADP-dependent aldehyde reductase
-
-
-
-
NADPH-aldehyde reductase
-
-
-
-
NADPH-cytochrome c reductase
NADPH-dependent aldehyde reductase
nonspecific succinic semialdehyde reductase
-
-
-
-
PyAeADHII
ScADHVI
short-chain dehydrogenase/reductase
Teth39_1597
TsAdh319
Y63 protein
YqhD
ADH
-
-
-
-
aldehyde reductase
-
-
-
-
NADPH-dependent aldehyde reductase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a primary alcohol + NADP+ = an aldehyde + NADPH + H+
a zinc protein. Some members of this group oxidize only primary alcohols, others act also on secondary alcohols. May be identical with EC 1.1.1.19 (L-glucuronate reductase), EC 1.1.1.33 [mevaldate reductase (NADPH2)] and EC 1.1.1.55 [lactaldehyde reductase (NADPH2)]. A-specific with respect to NADH2
-
a primary alcohol + NADP+ = an aldehyde + NADPH + H+
thermodynamics, standard electromotive force for reduction of NADP+ is -0.140 V
-
a primary alcohol + NADP+ = an aldehyde + NADPH + H+
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
reduction
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
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SYSTEMATIC NAME
IUBMB Comments
alcohol:NADP+ oxidoreductase
A zinc protein. Some members of this group oxidize only primary alcohols; others act also on secondary alcohols. May be identical with EC 1.1.1.19 (L-glucuronate reductase), EC 1.1.1.33 [mevaldate reductase (NADPH)] and EC 1.1.1.55 [lactaldehyde reductase (NADPH)]. Re-specific with respect to NADPH.
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CAS REGISTRY NUMBER
COMMENTARY
9028-12-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2E)-4-hydroxynon-2-enal + NADPH
(2E)-non-2-ene-1,4-diol + NADP+
-
30% of activity with cinnamaldehyde
-
-
r
(2E,4E)-hexa-2,4-dienal + NAD(P)H + H+
(2E,4E)-6-hydroxyhexa-2,4-dienal + NAD(P)+
-
-
-
-
-
(2R,3R)-2,3-butanediol + NADP+
(R)-2-acetoin + NADPH + H+
F8S123
the enzyme shows higher oxidation activities on the (2R,3R)-(-)-2,3-butanediol and meso-2,3-butanediol than on the (2S,3S)-(+)-2,3-butanediol, indicating that the enzyme predominantly oxidizes the R-hydroxyl group of 2,3-butanediol and minorly functions on the S-hydroxyl group. When meso-2,3-butanediol is oxidized, (S)-2-acetoin is the predominant product, with an enantiomeric excess (ee) of 88%, while oxidation of (2R,3R)-2,3-butanediol results in the production of (R)-2-acetoin with higher stereoselectivity (94% ee)
-
-
r
(2S)-methyl-1-butanol + NADP+
(2S)-methylbutanal + NADPH
-
39% activity compared to cinnamyl alcohol
-
-
r
(2S,3S)-2,3-butanediol + NADP+
(S)-2-acetoin + NADPH + H+
F8S123
the enzyme shows higher oxidation activities on the (2R,3R)-(-)-2,3-butanediol and meso-2,3-butanediol than on the (2S,3S)-(+)-2,3-butanediol, indicating that the enzyme predominantly oxidizes the R-hydroxyl group of 2,3-butanediol and minorly functions on the S-hydroxyl group. When meso-2,3-butanediol is oxidized, (S)-2-acetoin is the predominant product, with an enantiomeric excess (ee) of 88%, while oxidation of meso-2,3-butanediol results in the production of (R)-2-acetoin with higher stereoselectivity (94% ee)
-
-
r
(4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzaldehyde) + NADPH + H+
(4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzyl alcohol) + NADP+
-
eutypine, a toxin produced by Eutypa lata, the causal agent of Eutypa dieback in the grapevine Vitis vinifera
eutypinol, non-toxic metabolite
ir
(R)-1-phenyl-2-propanol + NADP+
phenylacetone + NADPH + H+
-
-
-
-
?
(R)-2-butanol + NADP+
2-butanone + NADPH + H+
the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol
-
-
?
(S)-1-phenyl-2-propanol + NADP+
phenylacetone + NADPH + H+
-
-
-
-
r
(S)-2-butanol + NADP+
2-butanone + NADPH + H+
the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol
-
-
?
(S)-2-butanol + NADP+
butanone + NADPH + H+
-
196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol
-
-
?
1,2-butanediol + NADP+
? + NADPH + H+
F8S123
25.6% of the activity compared to the substrate 2-butanol
-
-
?
1,3-butanediol + NADP+
? + NADPH + H+
F8S123
35.4% of the activity compared to the substrate 2-butanol
-
-
?
1-phenyl-2-butanone + NADPH + H+
(S)-1-phenyl-2-butanol + NADP+
-
-
-
-
?
1-propanol + NADP+
propanal + NADPH
-
16% activity compared to cinnamyl alcohol
-
-
r
2,2,2-trifluoro-1-phenylethanone + NADPH + H+
(1S)-2,2,2-trifluoro-1-phenylethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2,2-difluoro-1-phenylethanone + NADPH + H+
(1S)-2,2-difluoro-1-phenylethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2,4-pentanediol + NADP+
? + NADPH + H+
F8S123
9.2% of the activity compared to the substrate 2-butanol
-
-
?
2-(S)-methylbutanol + NADP+
(2S)-2-methylbutanal + NADPH
-
7% of activity with cinnamyl alcohol
-
-
r
2-butanol + NADP+
2-butanone + NADPH
-
2% of activity with cinnamyl alcohol
-
-
r
2-butanol + NADP+
2-butanone + NADPH + H+
F8S123
the enzyme does not possess stereoselectivity on 2-butanol or 2-butanone as substrate
-
-
r
2-butanol + NADP+
butanone + NADPH
-
1% activity compared to cinnamyl alcohol
-
-
r
2-butanone + NADPH + H+
2-butanol + NADP+
F8S123
the enzyme does not possess stereoselectivity on 2-butanol or 2-butanone as substrate
-
-
r
2-fluoro-1-(4-chlorophenyl)ethanone + NADPH + H+
(1S)-2-fluoro-1-(4-chlorophenyl)ethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-fluoro-1-(4-fluorophenyl)ethanone + NADPH + H+
(1S)-2-fluoro-1-(4-fluorophenyl)ethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-fluoro-1-(4-iodophenyl)ethanone + NADPH + H+
(1S)-2-fluoro-1-(4-iodophenyl)ethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-fluoro-1-phenylethanone + NADPH + H+
(1S)-2-fluoro-1-phenylethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-methylbutanol + NADP+
2-methylbutyraldehyde + NADPH + H+
-
24% activity with 1 mM 2-methylbutanol, 22% activity with 10 mM 2-methylbutanol
-
-
r
2-methylbutanol + NADP+
? + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 24% relative activity with 1 mM or 22% relative activity with 10 mM substrate
-
-
?
2-methylpropanol + NADP+
2-methylpropanal + NADPH
-
1% of activity with cinnamyl alcohol
-
-
r
2-pentanol + NADP+
pentan-2-one + NADPH + H+
-
67% of the activity with 2-propanol
-
-
?
2-pentanone + NADPH + H+
2-pentanol + NADP+
F8S123
86.2% of the activity compared to the substrate 2-butanone
-
-
r
2-phenylacetaldehyde + NADPH + H+
2-phenylethanol + NADP+
-
-
-
-
r
2-phenylethanol + NADP+
phenylacetaldehyde + NADPH
-
23% activity compared to cinnamyl alcohol
-
-
r
3,4-dihydroxyphenylacetaldehyde + NADPH + H+
3,4-dihydroxyphenylethanol + NADP+
-
-
-
-
r
3-methyl-1-butanol + NADP+
3-methylbutanal + NADPH
-
40% activity compared to cinnamyl alcohol
-
-
r
3-methylbutanal + NADPH
3-mehtyl-1-butanol + NADP+
-
84% of activity with cinnamaldehyde
-
-
r
3-methylbutanal + NADPH
3-methyl-1-butanol + NADP+
-
79% activity compared to cinnamaldehyde
-
-
r
3-methylbutanol + NADP+
3-methylbutanal + NADPH
-
15% of activity with cinnamyl alcohol
-
-
r
4-hydroxy-3-methoxybenzyl alcohol + NADP+
4-hydroxy-3-methoxybenzaldehyde + NADPH
-
-
-
-
r
4-hydroxybenzaldehyde + NADPH + H+
4-hydroxybenzyl alcohol + NADP+
-
-
-
-
?
4-phenyl-2-butanone + NADPH + H+
(S)-4-phenyl-2-butanol + NADP+
-
-
-
-
?
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly + NADH + H+
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly-al + NADPH + H+
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu- + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe-ol + NADP+
-
-
-
-
-
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly- + NADH + H+
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly-al + NAD+ + H2O
-
-
-
-
-
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly-al + NADPH + H+
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly-ol + NADP+
-
-
-
-
-
acetoin + NADPH + H+
2,3-butanediol + NADP+
F8S123
93% of the activity compared to the substrate 2-butanone. The racemic R/S-acetoin is used since neither R- nor S-acetoin is commercially available. Consistently, the reduction of racemic R/S-acetoin formed (2R,3R)-2,3-butanediol (presumably from R-acetoin) and meso-2,3-butanediol (presumably from S-acetoin) with extremely high stereoselectivity (99% ee over (2S,3S)-(+)-2,3-butanediol)
-
-
r
acetone + NADPH + H+
2-propanol + NADP+
F8S123
149% of the activity compared to the substrate 2-butanone
-
-
r
acetone + NADPH + H+
propan-2-ol + NADP+
-
about 13% of the activity with butan-2-ol
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
recombinant mutant G37D
recombinate mutant G37D
-
r
acetophenone + NADPH
(R)-1-phenylethanol + NADP+
-
catalysed by Escherichia coli coexpressing enzyme with NAD+-dependent formate dehydrogenase and pyridine nucleotide transhydrogenase genes pnta and pntb
-
-
?
acetophenone + NADPH + H+
1-phenylethanol + NADP+
-
recombinant wild-type
recombinant wild-type
-
r
aldehyde + NADPH + H+
alcohol + NADP+
-
the physiological role of this enzyme is likely to catalyze the reduction of aldehydes to alcohols
-
-
?
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 30% relative activity with 0.1 mM or 14% relative activity with 1 mM substrate
-
-
r
butanal + NADPH + H+
butanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 73% relative activity with 0.1 mM or 120% relative activity with 1 mM substrate
-
-
r
butane-1,2-diol + NADP+
butanal-2-ol + NADPH
-
2% activity compared to cinnamyl alcohol
-
-
r
butane-1,3-diol + NADP+
butanal-3-ol + NADPH
-
5% activity compared to cinnamyl alcohol
-
-
r
butane-1,4-diol + NADP+
butanal-4-ol + NADPH
-
8% activity compared to cinnamyl alcohol
-
-
r
butyraldehyde + NADPH + H+
1-butanol + NADP+
F8S123
112.4% of the activity compared to the substrate 2-butanone
-
-
r
cinnamaldehyde + NADPH
cinnamic alcohol + NADP+
5% of activity with acetaldehyde
-
-
r
cinnamaldehyde + NADPH + H+
cinnamyl alcohol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 61% relative activity with 0.1 mM or 7% relative activity with 1 mM substrate
-
-
r
cis-11-hexadecenal + NADPH
cis-11-hexadecenol + NADP+
-
87% relative activity
-
-
ir
coniferyl alcohol + NADP+
coniferaldehyde + NADPH + H+
-
7% activity compared to cinnamyl alcohol
-
-
r
coniferyl alcohol + NADP+
coniferylaldehyde + NADPH + H+
-
27% of activity with cinnamyl alcohol
-
-
r
cyclohexancarbaldehye + NADPH
cyclohexanol + NADP+
3% of activity with acetaldehyde
-
-
r
cyclohexanol + NADP+
cyclohexancarbaldehyde + NADPH
14% of activity with ethanol
-
-
r
cyclohexanol + NADP+
cyclohexanone + NADPH + H+
-
about 15% of the activity with butan-2-ol
-
-
?
D-arabinose + NADP+
D-arabitol + NADPH + H+
-
200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose
-
-
?
D-xylose + NADP+
? + NADPH + H+
-
246% of the activity with 2-propanol
-
-
?
decanal + NADPH
decanol + NADP+
-
no detectable catalysis of the reverse reaction, the oxidation of decanol by NADP+. 100% relative activity
-
-
ir
decanal + NADPH + H+
decanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 21% relative activity with 0.1 mM or 22% relative activity with 1 mM substrate
-
-
r
diacetyl + NADPH + H+
acetoin + NADP+
F8S123
the enzyme can not oxidize acetoin to diacetyl
-
-
ir
dimethylglyoxal + NADPH + H+
? + NADP+
-
270% of the activity with pyruvaldehyde
-
-
?
dodecanal + NADPH
dodecanol + NADP+
-
55% relative activity
-
-
ir
ethyl 4-bromo-3-oxobutanoate + NADPH
(R)-4-bromo-3-hydroxybutanoic acid ethyl ester + NADP+
-
-
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
(R)-4-chloro-3-hydroxybutanoic acid ethyl ester + NADP+
-
-
-
-
?
glutaraldehyde + NADPH
? + NADP+
5% of activity with acetaldehyde
-
-
r
hexyl alcohol + NADP+
?
the native enzyme shows 137.8% activity with hexyl alcohol compared to ethanol
-
-
?
isoamyl alcohol + NADP+
3-methylbutanol + NADPH
18% of activity with ethanol
-
-
r
isobutanol + NADP+
isobutanol + NADPH + H+
-
93% of the activity with ethanol
-
-
r
isobutanol + NADP+
isobutyl aldehyde + NADPH + H+
-
isoform AdhA, 221% of the activity with acetaldehyde
-
-
r
isobutyl aldehyde + NADPH + H+
isobutanol + NADP+
-
isoform AdhA, 469%, isoform AdhB, 48% of the activity with acetaldehyde
-
-
r
isopentanol + NADP+
3-methylbutanal + NADPH + H+
9% of activity with ethanol
-
-
r
isopropanol + NADP+
acetone + NADPH
2% of activity with ethanol
-
-
r
meso-2,3-butanediol + NADP+
(S)-2-acetoin + NADPH + H+
F8S123
the enzyme shows higher oxidation activities on the (2R,3R)-(-)-2,3-butanediol and meso-2,3-butanediol than on the (2S,3S)-(+)-2,3-butanediol, indicating that the enzyme predominantly oxidizes the R-hydroxyl group of 2,3-butanediol and minorly functions on the S-hydroxyl group. When meso-2,3-butanediol is oxidized, (S)-2-acetoin is the predominant product, with an enantiomeric excess (ee) of 88%, while oxidation of meso-2,3-butanediol results in the production of (R)-2-acetoin with higher stereoselectivity (94% ee)
-
-
r
methyl acetoacetate + NADH + H+
methyl (R)-3-hydroxy-butanoate + NAD+
-
-
-
-
?
methyl acetoacetate + NADPH + H+
methyl (R)-3-hydroxy-butanoate + NADP+
-
-
-
-
?
octanal + NADPH + H+
octanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 42% relative activity with 0.1 mM or 80% relative activity with 1 mM substrate
-
-
r
octanol + NADP+
octanal + NADPH
-
52% of activity with cinnamyl alcohol
-
-
r
octyl 4-chloro-3-oxobutanoate + NADPH
(R)-4-chloro-3-hydroxybutanoic acid ethyl ester + NADP+
-
-
-
-
?
pent-1-en-3-ol + NADP+
pent-1-en-3-one + NADPH + H+
-
good substrate
-
-
?
pentanal + NADPH + H+
pentanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 100% relative activity with 0.1 mM or 107% relative activity with 1 mM substrate
-
-
r
phenylacetone + NADPH + H+
(S)-1-phenyl-2-propanol + NADP+
-
-
-
-
r
phenylethanol + NADP+
? + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 15% relative activity with 1 mM or 24% relative activity with 10 mM substrate
-
-
?
phenylethanol + NADP+
phenylacetaldehyde + NADPH + H+
-
15% activity with 1 mM phenylethanol, 24% activity with 10 mM phenylethanol
-
-
r
propanal + NADPH + H+
propan-1-ol + NADP+
-
isoform AdhA, 50%, isoform AdhB, 62% of the activity with acetaldehyde
-
-
r
propanol + NADP+
propanal + NADPH + H+
-
21% activity with 1 mM propanol, 84% activity with 10 mM propanol
26% activity with 0.1 mM propanal, 87% activity with 1 mM propanal
-
r
salicylaldehyde + NADPH
salicylalcohol + NADP+
1% of activity with acetaldehyde
-
-
r
trans-2-nonenal + NADPH
trans-2-nonen-1-ol + NADP+
-
33% activity compared to cinnamaldehyde
-
-
r
trans-2-nonenal + NADPH
trans-2-nonenol + NADP+
-
39% of activity with cinnamaldehyde
-
-
r
1,3-propanediol + NADP+
? + NADPH
-
primary alcohol dehydrogenase
-
-
?
1-butanol + NADP+
butanal + NADPH
-
42% activity compared to cinnamyl alcohol
-
-
r
1-butanol + NADP+
butanal + NADPH + H+
-
169% of the activity compared to ethanol
-
-
r
1-butanol + NADP+
butanal + NADPH + H+
-
130% of the activity with ethanol
-
-
r
1-butanol + NADP+
butanal + NADPH + H+
-
130% of the activity with ethanol
-
-
r
1-butanol + NADP+
butanal + NADPH + H+
the native enzyme shows 154.6% activity with 1-butanol compared to ethanol
-
-
?
1-butanol + NADP+
butanal + NADPH + H+
the native enzyme shows 154.6% activity with 1-butanol compared to ethanol
-
-
?
1-butanol + NADP+
butyraldehyde + NADPH + H+
F8S123
5.1% of the activity compared to the substrate 2-butanol
-
-
r
1-heptanol + NADP+
heptanal + NADPH + H+
-
86% of the activity compared to ethanol
-
-
r
1-heptanol + NADP+
heptanal + NADPH + H+
-
114% of the activity with ethanol
-
-
r
1-heptanol + NADP+
heptanal + NADPH + H+
-
114% of the activity with ethanol
-
-
r
1-heptanol + NADP+
heptanal + NADPH + H+
the native enzyme shows 94% activity with 1-heptanol compared to ethanol
-
-
?
1-heptanol + NADP+
heptanal + NADPH + H+
the native enzyme shows 94% activity with 1-heptanol compared to ethanol
-
-
?
1-hexanol + NADP+
hexanal + NADPH
-
45% activity compared to cinnamyl alcohol
-
-
r
1-hexanol + NADP+
hexanal + NADPH + H+
-
95% of the activity compared to ethanol
-
-
r
1-hexanol + NADP+
hexanal + NADPH + H+
-
165% of the activity with ethanol
-
-
r
1-octanol + NADP+
octanal + NADPH
-
30% activity compared to cinnamyl alcohol
-
-
r
1-octanol + NADP+
octanal + NADPH + H+
-
57% of the activity compared to ethanol
-
-
r
1-octanol + NADP+
octanal + NADPH + H+
the native enzyme shows 68.8% activity with 1-octanol compared to ethanol
-
-
?
1-octanol + NADP+
octanal + NADPH + H+
the native enzyme shows 68.8% activity with 1-octanol compared to ethanol
-
-
?
1-pentanol + NADP+
n-pentanal + NADPH
-
primary alcohol dehydrogenase
-
-
r
1-pentanol + NADP+
pentanal + NADPH
-
46% activity compared to cinnamyl alcohol
-
-
r
1-pentanol + NADP+
pentanal + NADPH + H+
-
172% of the activity compared to ethanol
-
-
r
1-pentanol + NADP+
pentanal + NADPH + H+
-
135% of the activity with ethanol
-
-
r
1-pentanol + NADP+
pentanal + NADPH + H+
the native enzyme shows 184% activity with 1-pentanol compared to ethanol
-
-
?
1-pentanol + NADP+
pentanal + NADPH + H+
the native enzyme shows 184% activity with 1-pentanol compared to ethanol
-
-
?
1-propanol + NADP+
propanal + NADPH + H+
-
156% of the activity compared to ethanol
-
-
r
1-propanol + NADP+
propanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 21% relative activity with 1 mM or 84% relative activity with 10 mM substrate
-
-
r
1-propanol + NADP+
propanal + NADPH + H+
F8S123
15.1% of the activity compared to the substrate 2-butanol
-
-
?
1-propanol + NADP+
propanal + NADPH + H+
-
102% of the activity with ethanol
-
-
r
1-propanol + NADP+
propanal + NADPH + H+
the native enzyme shows 120.6% activity with 1-propanol compared to ethanol
-
-
?
2,3-pentanedione + NADPH + H+
? + NADP+
-
secondary alcohol dehydrogenase
-
-
?
2-butanol + NADP+
butan-2-one + NADPH
-
-
-
-
r
2-methoxybenzaldehyde + NADPH + H+
2-methoxybenzyl alcohol + NADP+
-
-
-
-
?
2-methyl-1-propanol + NADP+
2-methylpropanal + NADPH
-
28% activity compared to cinnamyl alcohol
-
-
r
2-methyl-1-propanol + NADP+
2-methylpropanal + NADPH
-
-
-
-
?
2-methylbutanal + NADPH
2-methyl-1-butanol + NADP+
-
89% activity compared to cinnamaldehyde
-
-
r
2-methylbutanal + NADPH
2-methyl-1-butanol + NADP+
-
92% of activity with cinnamaldehyde
-
-
r
2-methylpropanal + NADPH
2-methyl-1-propanol + NADP+
-
63% activity compared to cinnamaldehyde
-
-
r
2-methylpropanal + NADPH
2-methyl-1-propanol + NADP+
-
67% of activity with cinnamaldehyde
-
-
r
2-pentanol + NADP+
2-pentanone + NADPH + H+
maximal activity in oxidative reaction
-
-
r
2-pentanol + NADP+
2-pentanone + NADPH + H+
F8S123
66.3% of the activity compared to the substrate 2-butanol
-
-
r
2-phenylethanol + NADP+
2-phenylacetaldehyde + NADPH + H+
-
70% of the activity with ethanol
-
-
r
2-phenylethanol + NADP+
2-phenylacetaldehyde + NADPH + H+
the native enzyme shows 40.2% activity with 2-phenylethanol compared to ethanol
-
-
?
2-phenylethanol + NADP+
phenylacetaldehyde + NADPH + H+
-
76% of the activity compared to ethanol
-
-
r
2-propanol + NADP+
acetone + NADPH + H+
F8S123
88% of the activity compared to the substrate 2-butanol
-
-
r
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+
1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone
-
-
r
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+
1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone
-
-
r
3,4-dihydroxyphenylglycolaldehyde + NADPH
? + NADP+
-
low Km reductase
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
-
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
-
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
ir
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
-
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
-
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
-
-
-
-
?
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
r
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
r
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
r
5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+
(furan-2,5-diyl)dimethanol + NADP+
-
-
-
-
?
5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+
(furan-2,5-diyl)dimethanol + NADP+
-
-
-
-
?
5-hydroxymethylfurfural + NADPH + H+
5-hydroxymethylfurfuryl alcohol + NADP+
-
-
-
?
5-hydroxymethylfurfural + NADPH + H+
5-hydroxymethylfurfuryl alcohol + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 1% relative activity with 0.1 mM or 23% relative activity with 1 mM substrate
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
anisaldehyde + NADPH
4-methoxybenzyl alcohol + NADP+
-
72% of activity with cinnamaldehyde
-
-
r
anisaldehyde + NADPH
4-methoxybenzyl alcohol + NADP+
-
83% activity compared to cinnamaldehyde
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
8% of activity with acetaldehyde
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
45% activity compared to cinnamaldehyde
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
72% of activity with cinnamaldehyde
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH
-
45% of activity with cinnamyl alcohol
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH
-
78% activity compared to cinnamyl alcohol
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 42% relative activity with 1 mM or 79% relative activity with 10 mM substrate
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
42% activity with 1 mM benzyl alcohol, 79% activity with 10 mM benzyl alcohol
30% activity with 0.1 mM benzaldehyde, 14% activity with 1 mM benzaldehyde
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
180% of the activity with 2-propanol
-
-
?
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
180% of the activity with 2-propanol
-
-
?
butan-1-ol + NADP+
butanal + NADPH + H+
-
isoform ADH-I, 94%, isoform ADH II, 32% of the actvity with hexan-1-ol
-
-
?
butan-1-ol + NADP+
butanal + NADPH + H+
-
isoform ADH-I, 94%, isoform ADH II, 32% of the actvity with hexan-1-ol
-
-
?
butan-1-ol + NADP+
butanal + NADPH + H+
-
isoform AdhA, 238% of the activity with acetaldehyde
-
-
r
butan-2-ol + NADP+
butanone + NADPH + H+
-
isoform ADH-I, 89%, isoform ADH II, 23% of the actvity with hexan-1-ol
-
-
?
butan-2-ol + NADP+
butanone + NADPH + H+
-
isoform ADH-I, 89%, isoform ADH II, 23% of the actvity with hexan-1-ol
-
-
?
butanal + NADPH + H+
1-butanol + NADP+
47% of activity with acetaldehyde
-
-
r
butanal + NADPH + H+
1-butanol + NADP+
-
19% activity compared to cinnamaldehyde
-
-
r
butanal + NADPH + H+
1-butanol + NADP+
-
57% of activity with cinnamaldehyde
-
-
r
butanal + NADPH + H+
butan-1-ol + NADP+
-
isoform AdhA, 625%, isoform AdhB, 108% of the activity with acetaldehyde
-
-
r
butanol + NADP+
butanal + NADPH
68% of activity with ethanol
-
-
r
butanol + NADP+
butanal + NADPH
-
24% of activity with cinnamyl alcohol
-
-
r
butanol + NADP+
butanal + NADPH + H+
-
23% activity with 1 mM butanol, 70% activity with 10 mM butanol
73% activity with 0.1 mM butanal, 120% activity with 1 mM butanal
-
r
butanol + NADP+
butanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 23% relative activity with 1 mM or 70% relative activity with 10 mM substrate
-
-
r
cellobiose + NADP+
? + NADPH + H+
-
71% of the activity with 2-propanol
-
-
?
cellobiose + NADP+
? + NADPH + H+
-
71% of the activity with 2-propanol
-
-
?
cinnamyl alcohol + NADP+
cinnamaldehyde + NADPH + H+
-
100% activity with 1 mM cinnamyl alcohol, 116% activity with 10 mM hexanol
61% activity with 0.1 mM cinnamaldehyde, 7% activity with 1 mM cinnamaldehyde
-
r
cinnamyl alcohol + NADP+
cinnamaldehyde + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 100% relative activity with 1 mM or 116% relative activity with 10 mM substrate
-
-
r
coniferaldehyde + NADPH
coniferyl alcohol + NADP+
-
10% of activity with cinnamaldehyde
-
-
r
coniferaldehyde + NADPH
coniferyl alcohol + NADP+
-
15% activity compared to cinnamaldehyde
-
-
r
coniferaldehyde + NADPH
coniferyl alcohol + NADP+
-
converted less efficiently than eutypine
-
-
?
D-glucose + NADP+
? + NADPH + H+
-
146% of the activity with 2-propanol
-
-
?
D-glucose + NADP+
? + NADPH + H+
-
146% of the activity with 2-propanol
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH + H+
?
0.1 M phosphate buffer, pH 7.0
-
-
?
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
ir
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
286165, 286167, 286168, 286171, 286172, 286174, 286176, 286177, 286178, 286179, 286181, 286182, 286196
-
-
r
decanol + NADP+
decanal + NADPH + H+
-
3% activity with 1 mM decanol, 6% activity with 10 mM decanol
21% activity with 0.1 mM decanal, 22% activity with 1 mM decanal
-
r
decanol + NADP+
decanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 3% relative activity with 1 mM or 6% relative activity with 10 mM substrate
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
kcat/Km for NAD+ is 1900fold lower compared to NADP+
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
isoform AdhB: no oxidation of ethanol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
kcat/Km for NAD+ is 1900fold lower compared to NADP+
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
2% activity compared to cinnamyl alcohol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
4% of activity with cinnamyl alcohol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
19% activity with 1 mM ethanol, 91% activity with 10 mM ethanol
1% activity with 0.1 mM acetaldehyde, 23% activity with 1 mM acetaldehyde
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 19% relative activity with 1 mM or 91% relative activity with 10 mM substrate
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
F8S123
5.7% of the activity compared to the substrate 2-butanol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
the native enzyme shows 100% activity with ethanol
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
the native enzyme shows 100% activity with ethanol
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl-4-chloro-3-oxobutanoate + NADPH + H+
ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl-4-chloro-3-oxobutanoate + NADPH + H+
ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl-4-chloro-3-oxobutanoate + NADPH + H+
ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethylacetoacetate + NADPH
ethyl L-(+)-3-hydroxybutyrate + NADP+
-
-
-
-
?
formaldehyde + NADPH
methanol + NADP+
6% of activity with acetaldehyde
-
-
ir
formaldehyde + NADPH + H+
methanol + NADP+
-
-
-
?
furfural + NADH + H+
furfuryl alcohol + NAD+
-
-
-
?
furfural + NADPH
furfuryl alcohol + NADP+
-
27% of activity with cinnamaldehyde
-
-
r
furfural + NADPH
furfuryl alcohol + NADP+
-
54% activity compared to cinnamaldehyde
-
-
r
furfural + NADPH + H+
furfuryl alcohol + NADP+
-
-
-
?
furfural + NADPH + H+
furfuryl alcohol + NADP+
-
-
-
?
heptanal + NADPH
1-heptanol + NADP+
-
86% activity compared to cinnamaldehyde
-
-
r
heptanal + NADPH
1-heptanol + NADP+
-
88% of activity with cinnamaldehyde
-
-
r
hexanal + NADPH + H+
1-hexanol + NADP+
-
88% activity compared to cinnamaldehyde
-
-
r
hexanal + NADPH + H+
1-hexanol + NADP+
-
89% of activity with cinnamaldehyde
-
-
r
hexanal + NADPH + H+
hexanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 53% relative activity with 0.1 mM or 100% relative activity with 1 mM substrate
-
-
r
hexanol + NADP+
hexanal + NADPH
60% of activity with ethanol
-
-
r
hexanol + NADP+
hexanal + NADPH
-
154% of activity with cinnamyl alcohol
-
-
r
hexanol + NADP+
hexanal + NADPH + H+
-
27% activity with 1 mM hexanol, 40% activity with 10 mM hexanol
53% activity with 0.1 mM hexanal, 100% activity with 1 mM hexanal
-
r
hexanol + NADP+
hexanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 27% relative activity with 1 mM or 40% relative activity with 10 mM substrate
-
-
r
isobutanol + NADP+
isobutanal + NADPH + H+
-
122% of the activity compared to ethanol
-
-
r