HOME
login
history
all enzymes
BRENDA home
History
Information on EC 1.1.1.2 - alcohol dehydrogenase (NADP+)
for references in articles please use BRENDA:EC1.1.1.2Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.1.1.2 alcohol dehydrogenase (NADP+)IUBMB Comments
A zinc protein. Some members of this group oxidize only primary alcohols; others act also on secondary alcohols. May be identical with EC 1.1.1.19 (L-glucuronate reductase), EC 1.1.1.33 [mevaldate reductase (NADPH)] and EC 1.1.1.55 [lactaldehyde reductase (NADPH)]. Re-specific with respect to NADPH.
Specify your search results
Word Map
- 1.1.1.2
- microsomal
- horse
- dehydrogenases
- reductases
- steroid
- aldose
- nad+
- hydroxylase
- akr1c3
- aminopyrine
- n-demethylase
- aniline
- akr1b10
- nicotinamide
-
xenobiotic
- pyrazole
-
nadh-cytochrome
-
drug-metabolizing
-
1.1.1.1
- acetaldehyde
-
hydride
-
benzoapyrene
-
hydroxysteroids
- 3-methylcholanthrene
-
dihydrodiols
- 4-methylpyrazole
-
mixed-function
-
p-450-dependent
- 7-ethoxycoumarin
-
3alpha-hydroxysteroids
- benzphetamine
- ethylmorphine
-
n-demethylation
-
anion-binding
- o-quinones
- synthesis
-
17beta-hydroxysteroids
-
p-nitroanisole
-
3alpha
- 7-ethoxyresorufin
- analysis
- 17beta-hsds
- tolrestat
- industry
- sorbinil
-
phosphorescence
- 5alpha-dihydrotestosterone
- agriculture
- hexobarbital
-
zinc-bound
- medicine
-
udp-glucuronyltransferase
- ketosteroid
-
coenzyme-binding
- trifluoroethanol
The enzyme appears in viruses and cellular organisms
Synonyms
2° Adh, 3-DG-reducing enzyme, AAur_2040, ADH, ADH-1, ADH-2, ADH-I, ADH-II, ADH2, Adh319, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2° Adh
3-DG-reducing enzyme
-
-
-
-
AAur_2040
ADH
ADH-1
ADH-2
ADH-I
ADH-II
Adh319
ADH4
Adh6
AdhA
AKR1A1
alcohol dehydrogenase [NADP(+)]
Alcohol dehydrogenase [NADP+]
-
-
-
-
aldehyde reductase
aldehyde reductase (NADPH2)
-
-
-
-
Aldo-keto reductase family 1 member A1
-
-
-
-
aldose reductase
ALR
-
-
-
-
ALR 1
-
-
-
-
ALR1
BdhA
daunorubicin reductase
hexogenate dehydrogenase
high-Km aldehyde reductase
-
-
-
-
L-hexonate dehydrogenase
low-Km aldehyde reductase
-
-
-
-
Mpd1
NADP-alcohol dehydrogenase
-
-
-
-
NADP-aldehyde reductase
-
-
-
-
NADP-dependent aldehyde reductase
-
-
-
-
NADPH-aldehyde reductase
-
-
-
-
NADPH-cytochrome c reductase
NADPH-dependent aldehyde reductase
nonspecific succinic semialdehyde reductase
-
-
-
-
PyAeADHII
ScADHVI
short-chain dehydrogenase/reductase
Teth39_1597
TsAdh319
Y63 protein
YqhD
ADH
-
-
-
-
aldehyde reductase
-
-
-
-
NADPH-dependent aldehyde reductase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a primary alcohol + NADP+ = an aldehyde + NADPH + H+
a zinc protein. Some members of this group oxidize only primary alcohols, others act also on secondary alcohols. May be identical with EC 1.1.1.19 (L-glucuronate reductase), EC 1.1.1.33 [mevaldate reductase (NADPH2)] and EC 1.1.1.55 [lactaldehyde reductase (NADPH2)]. A-specific with respect to NADH2
-
a primary alcohol + NADP+ = an aldehyde + NADPH + H+
-
-
-
-
a primary alcohol + NADP+ = an aldehyde + NADPH + H+
thermodynamics, standard electromotive force for reduction of NADP+ is -0.140 V
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
reduction
redox reaction
-
-
-
-
reduction
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
alcohol:NADP+ oxidoreductase
A zinc protein. Some members of this group oxidize only primary alcohols; others act also on secondary alcohols. May be identical with EC 1.1.1.19 (L-glucuronate reductase), EC 1.1.1.33 [mevaldate reductase (NADPH)] and EC 1.1.1.55 [lactaldehyde reductase (NADPH)]. Re-specific with respect to NADPH.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9028-12-0
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2E)-4-hydroxynon-2-enal + NADPH
(2E)-non-2-ene-1,4-diol + NADP+
-
30% of activity with cinnamaldehyde
-
-
r
(2E,4E)-hexa-2,4-dienal + NAD(P)H + H+
(2E,4E)-6-hydroxyhexa-2,4-dienal + NAD(P)+
-
-
-
-
-
(2R,3R)-2,3-butanediol + NADP+
(R)-2-acetoin + NADPH + H+
the enzyme shows higher oxidation activities on the (2R,3R)-(-)-2,3-butanediol and meso-2,3-butanediol than on the (2S,3S)-(+)-2,3-butanediol, indicating that the enzyme predominantly oxidizes the R-hydroxyl group of 2,3-butanediol and minorly functions on the S-hydroxyl group. When meso-2,3-butanediol is oxidized, (S)-2-acetoin is the predominant product, with an enantiomeric excess (ee) of 88%, while oxidation of (2R,3R)-2,3-butanediol results in the production of (R)-2-acetoin with higher stereoselectivity (94% ee)
-
-
r
(2S)-methyl-1-butanol + NADP+
(2S)-methylbutanal + NADPH
-
39% activity compared to cinnamyl alcohol
-
-
r
(2S,3S)-2,3-butanediol + NADP+
(S)-2-acetoin + NADPH + H+
the enzyme shows higher oxidation activities on the (2R,3R)-(-)-2,3-butanediol and meso-2,3-butanediol than on the (2S,3S)-(+)-2,3-butanediol, indicating that the enzyme predominantly oxidizes the R-hydroxyl group of 2,3-butanediol and minorly functions on the S-hydroxyl group. When meso-2,3-butanediol is oxidized, (S)-2-acetoin is the predominant product, with an enantiomeric excess (ee) of 88%, while oxidation of meso-2,3-butanediol results in the production of (R)-2-acetoin with higher stereoselectivity (94% ee)
-
-
r
(4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzaldehyde) + NADPH + H+
(4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzyl alcohol) + NADP+
-
eutypine, a toxin produced by Eutypa lata, the causal agent of Eutypa dieback in the grapevine Vitis vinifera
eutypinol, non-toxic metabolite
ir
(R)-1-phenyl-2-propanol + NADP+
phenylacetone + NADPH + H+
-
-
-
-
?
(R)-2-butanol + NADP+
2-butanone + NADPH + H+
the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol
-
-
?
(S)-1-phenyl-2-propanol + NADP+
phenylacetone + NADPH + H+
-
-
-
-
r
(S)-2-butanol + NADP+
2-butanone + NADPH + H+
the enzyme shows a slight preference for the (S)-2-butanol over (R)-2-butanol
-
-
?
(S)-2-butanol + NADP+
butanone + NADPH + H+
-
196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol
-
-
?
1,2-butanediol + NADP+
? + NADPH + H+
25.6% of the activity compared to the substrate 2-butanol
-
-
?
1,3-butanediol + NADP+
? + NADPH + H+
35.4% of the activity compared to the substrate 2-butanol
-
-
?
1-phenyl-2-butanone + NADPH + H+
(S)-1-phenyl-2-butanol + NADP+
-
-
-
-
?
1-propanol + NADP+
propanal + NADPH
-
16% activity compared to cinnamyl alcohol
-
-
r
2,2,2-trifluoro-1-phenylethanone + NADPH + H+
(1S)-2,2,2-trifluoro-1-phenylethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2,2-difluoro-1-phenylethanone + NADPH + H+
(1S)-2,2-difluoro-1-phenylethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2,4-pentanediol + NADP+
? + NADPH + H+
9.2% of the activity compared to the substrate 2-butanol
-
-
?
2-(S)-methylbutanol + NADP+
(2S)-2-methylbutanal + NADPH
-
7% of activity with cinnamyl alcohol
-
-
r
2-butanol + NADP+
2-butanone + NADPH
-
2% of activity with cinnamyl alcohol
-
-
r
2-butanol + NADP+
2-butanone + NADPH + H+
the enzyme does not possess stereoselectivity on 2-butanol or 2-butanone as substrate
-
-
r
2-butanol + NADP+
butanone + NADPH
-
1% activity compared to cinnamyl alcohol
-
-
r
2-butanone + NADPH + H+
2-butanol + NADP+
the enzyme does not possess stereoselectivity on 2-butanol or 2-butanone as substrate
-
-
r
2-fluoro-1-(4-chlorophenyl)ethanone + NADPH + H+
(1S)-2-fluoro-1-(4-chlorophenyl)ethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-fluoro-1-(4-fluorophenyl)ethanone + NADPH + H+
(1S)-2-fluoro-1-(4-fluorophenyl)ethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-fluoro-1-(4-iodophenyl)ethanone + NADPH + H+
(1S)-2-fluoro-1-(4-iodophenyl)ethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-fluoro-1-phenylethanone + NADPH + H+
(1S)-2-fluoro-1-phenylethanol + NADP+
-
-
99% conversion, 99% enantiomeric excess
-
?
2-methylbutanol + NADP+
2-methylbutyraldehyde + NADPH + H+
-
24% activity with 1 mM 2-methylbutanol, 22% activity with 10 mM 2-methylbutanol
-
-
r
2-methylbutanol + NADP+
? + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 24% relative activity with 1 mM or 22% relative activity with 10 mM substrate
-
-
?
2-methylpropanol + NADP+
2-methylpropanal + NADPH
-
1% of activity with cinnamyl alcohol
-
-
r
2-pentanol + NADP+
pentan-2-one + NADPH + H+
-
67% of the activity with 2-propanol
-
-
?
2-pentanone + NADPH + H+
2-pentanol + NADP+
86.2% of the activity compared to the substrate 2-butanone
-
-
r
2-phenylacetaldehyde + NADPH + H+
2-phenylethanol + NADP+
-
-
-
-
r
2-phenylethanol + NADP+
phenylacetaldehyde + NADPH
-
23% activity compared to cinnamyl alcohol
-
-
r
3,4-dihydroxyphenylacetaldehyde + NADPH + H+
3,4-dihydroxyphenylethanol + NADP+
-
-
-
-
r
3-methyl-1-butanol + NADP+
3-methylbutanal + NADPH
-
40% activity compared to cinnamyl alcohol
-
-
r
3-methylbutanal + NADPH
3-mehtyl-1-butanol + NADP+
-
84% of activity with cinnamaldehyde
-
-
r
3-methylbutanal + NADPH
3-methyl-1-butanol + NADP+
-
79% activity compared to cinnamaldehyde
-
-
r
3-methylbutanol + NADP+
3-methylbutanal + NADPH
-
15% of activity with cinnamyl alcohol
-
-
r
4-hydroxy-3-methoxybenzyl alcohol + NADP+
4-hydroxy-3-methoxybenzaldehyde + NADPH
-
-
-
-
r
4-hydroxybenzaldehyde + NADPH + H+
4-hydroxybenzyl alcohol + NADP+
-
-
-
-
?
4-phenyl-2-butanone + NADPH + H+
(S)-4-phenyl-2-butanol + NADP+
-
-
-
-
?
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly + NADH + H+
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly-al + NADPH + H+
Ac-D-Leu-D-Trp-D-Leu-D-Trp-Gly-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-D-Ala-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-Gly-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Ala-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Asp-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu- + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Leu-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Lys-ol + NADP+
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe + NADH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe-al + NAD+ + H2O
-
-
-
-
-
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe-al + NADPH + H+
Ac-D-Leu-L-Trp-D-Leu-L-Trp-L-Phe-ol + NADP+
-
-
-
-
-
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly- + NADH + H+
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly-al + NAD+ + H2O
-
-
-
-
-
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly-al + NADPH + H+
Ac-L-Leu-L-Trp-L-Leu-L-Trp-Gly-ol + NADP+
-
-
-
-
-
acetoin + NADPH + H+
2,3-butanediol + NADP+
93% of the activity compared to the substrate 2-butanone. The racemic R/S-acetoin is used since neither R- nor S-acetoin is commercially available. Consistently, the reduction of racemic R/S-acetoin formed (2R,3R)-2,3-butanediol (presumably from R-acetoin) and meso-2,3-butanediol (presumably from S-acetoin) with extremely high stereoselectivity (99% ee over (2S,3S)-(+)-2,3-butanediol)
-
-
r
acetone + NADPH + H+
2-propanol + NADP+
149% of the activity compared to the substrate 2-butanone
-
-
r
acetone + NADPH + H+
propan-2-ol + NADP+
-
about 13% of the activity with butan-2-ol
-
-
?
acetophenone + NADH + H+
1-phenylethanol + NAD+
-
recombinant mutant G37D
recombinate mutant G37D
-
r
acetophenone + NADPH
(R)-1-phenylethanol + NADP+
-
catalysed by Escherichia coli coexpressing enzyme with NAD+-dependent formate dehydrogenase and pyridine nucleotide transhydrogenase genes pnta and pntb
-
-
?
acetophenone + NADPH + H+
1-phenylethanol + NADP+
-
recombinant wild-type
recombinant wild-type
-
r
aldehyde + NADPH + H+
alcohol + NADP+
-
the physiological role of this enzyme is likely to catalyze the reduction of aldehydes to alcohols
-
-
?
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 30% relative activity with 0.1 mM or 14% relative activity with 1 mM substrate
-
-
r
butanal + NADPH + H+
butanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 73% relative activity with 0.1 mM or 120% relative activity with 1 mM substrate
-
-
r
butane-1,2-diol + NADP+
butanal-2-ol + NADPH
-
2% activity compared to cinnamyl alcohol
-
-
r
butane-1,3-diol + NADP+
butanal-3-ol + NADPH
-
5% activity compared to cinnamyl alcohol
-
-
r
butane-1,4-diol + NADP+
butanal-4-ol + NADPH
-
8% activity compared to cinnamyl alcohol
-
-
r
butyraldehyde + NADPH + H+
1-butanol + NADP+
112.4% of the activity compared to the substrate 2-butanone
-
-
r
cinnamaldehyde + NADPH
cinnamic alcohol + NADP+
5% of activity with acetaldehyde
-
-
r
cinnamaldehyde + NADPH + H+
cinnamyl alcohol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 61% relative activity with 0.1 mM or 7% relative activity with 1 mM substrate
-
-
r
cis-11-hexadecenal + NADPH
cis-11-hexadecenol + NADP+
-
87% relative activity
-
-
ir
coniferyl alcohol + NADP+
coniferaldehyde + NADPH + H+
-
7% activity compared to cinnamyl alcohol
-
-
r
coniferyl alcohol + NADP+
coniferylaldehyde + NADPH + H+
-
27% of activity with cinnamyl alcohol
-
-
r
cyclohexancarbaldehye + NADPH
cyclohexanol + NADP+
3% of activity with acetaldehyde
-
-
r
cyclohexanol + NADP+
cyclohexancarbaldehyde + NADPH
14% of activity with ethanol
-
-
r
cyclohexanol + NADP+
cyclohexanone + NADPH + H+
-
about 15% of the activity with butan-2-ol
-
-
?
D-arabinose + NADP+
D-arabitol + NADPH + H+
-
200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose
-
-
?
D-xylose + NADP+
? + NADPH + H+
-
246% of the activity with 2-propanol
-
-
?
decanal + NADPH
decanol + NADP+
-
no detectable catalysis of the reverse reaction, the oxidation of decanol by NADP+. 100% relative activity
-
-
ir
decanal + NADPH + H+
decanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 21% relative activity with 0.1 mM or 22% relative activity with 1 mM substrate
-
-
r
diacetyl + NADPH + H+
acetoin + NADP+
the enzyme can not oxidize acetoin to diacetyl
-
-
ir
dimethylglyoxal + NADPH + H+
? + NADP+
-
270% of the activity with pyruvaldehyde
-
-
?
dodecanal + NADPH
dodecanol + NADP+
-
55% relative activity
-
-
ir
ethyl 4-bromo-3-oxobutanoate + NADPH
(R)-4-bromo-3-hydroxybutanoic acid ethyl ester + NADP+
-
-
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
(R)-4-chloro-3-hydroxybutanoic acid ethyl ester + NADP+
-
-
-
-
?
glutaraldehyde + NADPH
? + NADP+
5% of activity with acetaldehyde
-
-
r
hexyl alcohol + NADP+
?
the native enzyme shows 137.8% activity with hexyl alcohol compared to ethanol
-
-
?
isoamyl alcohol + NADP+
3-methylbutanol + NADPH
18% of activity with ethanol
-
-
r
isobutanol + NADP+
isobutanol + NADPH + H+
-
93% of the activity with ethanol
-
-
r
isobutanol + NADP+
isobutyl aldehyde + NADPH + H+
-
isoform AdhA, 221% of the activity with acetaldehyde
-
-
r
isobutyl aldehyde + NADPH + H+
isobutanol + NADP+
-
isoform AdhA, 469%, isoform AdhB, 48% of the activity with acetaldehyde
-
-
r
isopentanol + NADP+
3-methylbutanal + NADPH + H+
9% of activity with ethanol
-
-
r
isopropanol + NADP+
acetone + NADPH
2% of activity with ethanol
-
-
r
meso-2,3-butanediol + NADP+
(S)-2-acetoin + NADPH + H+
the enzyme shows higher oxidation activities on the (2R,3R)-(-)-2,3-butanediol and meso-2,3-butanediol than on the (2S,3S)-(+)-2,3-butanediol, indicating that the enzyme predominantly oxidizes the R-hydroxyl group of 2,3-butanediol and minorly functions on the S-hydroxyl group. When meso-2,3-butanediol is oxidized, (S)-2-acetoin is the predominant product, with an enantiomeric excess (ee) of 88%, while oxidation of meso-2,3-butanediol results in the production of (R)-2-acetoin with higher stereoselectivity (94% ee)
-
-
r
methyl acetoacetate + NADH + H+
methyl (R)-3-hydroxy-butanoate + NAD+
-
-
-
-
?
methyl acetoacetate + NADPH + H+
methyl (R)-3-hydroxy-butanoate + NADP+
-
-
-
-
?
octanal + NADPH + H+
octanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 42% relative activity with 0.1 mM or 80% relative activity with 1 mM substrate
-
-
r
octanol + NADP+
octanal + NADPH
-
52% of activity with cinnamyl alcohol
-
-
r
octyl 4-chloro-3-oxobutanoate + NADPH
(R)-4-chloro-3-hydroxybutanoic acid ethyl ester + NADP+
-
-
-
-
?
pent-1-en-3-ol + NADP+
pent-1-en-3-one + NADPH + H+
-
good substrate
-
-
?
pentanal + NADPH + H+
pentanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 100% relative activity with 0.1 mM or 107% relative activity with 1 mM substrate
-
-
r
phenylacetone + NADPH + H+
(S)-1-phenyl-2-propanol + NADP+
-
-
-
-
r
phenylethanol + NADP+
? + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 15% relative activity with 1 mM or 24% relative activity with 10 mM substrate
-
-
?
phenylethanol + NADP+
phenylacetaldehyde + NADPH + H+
-
15% activity with 1 mM phenylethanol, 24% activity with 10 mM phenylethanol
-
-
r
propanal + NADPH + H+
propan-1-ol + NADP+
-
isoform AdhA, 50%, isoform AdhB, 62% of the activity with acetaldehyde
-
-
r
propanol + NADP+
propanal + NADPH + H+
-
21% activity with 1 mM propanol, 84% activity with 10 mM propanol
26% activity with 0.1 mM propanal, 87% activity with 1 mM propanal
-
r
salicylaldehyde + NADPH
salicylalcohol + NADP+
1% of activity with acetaldehyde
-
-
r
trans-2-nonenal + NADPH
trans-2-nonen-1-ol + NADP+
-
33% activity compared to cinnamaldehyde
-
-
r
trans-2-nonenal + NADPH
trans-2-nonenol + NADP+
-
39% of activity with cinnamaldehyde
-
-
r
1,3-propanediol + NADP+
? + NADPH
-
primary alcohol dehydrogenase
-
-
?
1-butanol + NADP+
butanal + NADPH
-
42% activity compared to cinnamyl alcohol
-
-
r
1-butanol + NADP+
butanal + NADPH + H+
-
169% of the activity compared to ethanol
-
-
r
1-butanol + NADP+
butanal + NADPH + H+
-
130% of the activity with ethanol
-
-
r
1-butanol + NADP+
butanal + NADPH + H+
-
130% of the activity with ethanol
-
-
r
1-butanol + NADP+
butanal + NADPH + H+
the native enzyme shows 154.6% activity with 1-butanol compared to ethanol
-
-
?
1-butanol + NADP+
butanal + NADPH + H+
the native enzyme shows 154.6% activity with 1-butanol compared to ethanol
-
-
?
1-butanol + NADP+
butyraldehyde + NADPH + H+
5.1% of the activity compared to the substrate 2-butanol
-
-
r
1-heptanol + NADP+
heptanal + NADPH + H+
-
86% of the activity compared to ethanol
-
-
r
1-heptanol + NADP+
heptanal + NADPH + H+
-
114% of the activity with ethanol
-
-
r
1-heptanol + NADP+
heptanal + NADPH + H+
-
114% of the activity with ethanol
-
-
r
1-heptanol + NADP+
heptanal + NADPH + H+
the native enzyme shows 94% activity with 1-heptanol compared to ethanol
-
-
?
1-heptanol + NADP+
heptanal + NADPH + H+
the native enzyme shows 94% activity with 1-heptanol compared to ethanol
-
-
?
1-hexanol + NADP+
hexanal + NADPH
-
45% activity compared to cinnamyl alcohol
-
-
r
1-hexanol + NADP+
hexanal + NADPH + H+
-
95% of the activity compared to ethanol
-
-
r
1-hexanol + NADP+
hexanal + NADPH + H+
-
165% of the activity with ethanol
-
-
r
1-octanol + NADP+
octanal + NADPH
-
30% activity compared to cinnamyl alcohol
-
-
r
1-octanol + NADP+
octanal + NADPH + H+
-
57% of the activity compared to ethanol
-
-
r
1-octanol + NADP+
octanal + NADPH + H+
the native enzyme shows 68.8% activity with 1-octanol compared to ethanol
-
-
?
1-octanol + NADP+
octanal + NADPH + H+
the native enzyme shows 68.8% activity with 1-octanol compared to ethanol
-
-
?
1-pentanol + NADP+
n-pentanal + NADPH
-
primary alcohol dehydrogenase
-
-
r
1-pentanol + NADP+
pentanal + NADPH
-
46% activity compared to cinnamyl alcohol
-
-
r
1-pentanol + NADP+
pentanal + NADPH + H+
-
172% of the activity compared to ethanol
-
-
r
1-pentanol + NADP+
pentanal + NADPH + H+
-
135% of the activity with ethanol
-
-
r
1-pentanol + NADP+
pentanal + NADPH + H+
the native enzyme shows 184% activity with 1-pentanol compared to ethanol
-
-
?
1-pentanol + NADP+
pentanal + NADPH + H+
the native enzyme shows 184% activity with 1-pentanol compared to ethanol
-
-
?
1-propanol + NADP+
propanal + NADPH + H+
-
156% of the activity compared to ethanol
-
-
r
1-propanol + NADP+
propanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 21% relative activity with 1 mM or 84% relative activity with 10 mM substrate
-
-
r
1-propanol + NADP+
propanal + NADPH + H+
15.1% of the activity compared to the substrate 2-butanol
-
-
?
1-propanol + NADP+
propanal + NADPH + H+
-
102% of the activity with ethanol
-
-
r
1-propanol + NADP+
propanal + NADPH + H+
the native enzyme shows 120.6% activity with 1-propanol compared to ethanol
-
-
?
2,3-pentanedione + NADPH + H+
? + NADP+
-
secondary alcohol dehydrogenase
-
-
?
2-butanol + NADP+
butan-2-one + NADPH
-
-
-
-
r
2-methoxybenzaldehyde + NADPH + H+
2-methoxybenzyl alcohol + NADP+
-
-
-
-
?
2-methyl-1-propanol + NADP+
2-methylpropanal + NADPH
-
28% activity compared to cinnamyl alcohol
-
-
r
2-methyl-1-propanol + NADP+
2-methylpropanal + NADPH
-
-
-
-
?
2-methylbutanal + NADPH
2-methyl-1-butanol + NADP+
-
89% activity compared to cinnamaldehyde
-
-
r
2-methylbutanal + NADPH
2-methyl-1-butanol + NADP+
-
92% of activity with cinnamaldehyde
-
-
r
2-methylpropanal + NADPH
2-methyl-1-propanol + NADP+
-
63% activity compared to cinnamaldehyde
-
-
r
2-methylpropanal + NADPH
2-methyl-1-propanol + NADP+
-
67% of activity with cinnamaldehyde
-
-
r
2-pentanol + NADP+
2-pentanone + NADPH + H+
maximal activity in oxidative reaction
-
-
r
2-pentanol + NADP+
2-pentanone + NADPH + H+
66.3% of the activity compared to the substrate 2-butanol
-
-
r
2-phenylethanol + NADP+
2-phenylacetaldehyde + NADPH + H+
-
70% of the activity with ethanol
-
-
r
2-phenylethanol + NADP+
2-phenylacetaldehyde + NADPH + H+
the native enzyme shows 40.2% activity with 2-phenylethanol compared to ethanol
-
-
?
2-phenylethanol + NADP+
phenylacetaldehyde + NADPH + H+
-
76% of the activity compared to ethanol
-
-
r
2-propanol + NADP+
acetone + NADPH + H+
88% of the activity compared to the substrate 2-butanol
-
-
r
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+
1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone
-
-
r
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+
1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone
-
-
r
3,4-dihydroxyphenylglycolaldehyde + NADPH
? + NADP+
-
low Km reductase
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
-
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
-
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
ir
3-pyridinecarboxaldehyde + NADPH
3-pyridinemethanol + NADP+
-
-
-
-
r
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
-
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
-
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
-
-
-
-
?
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
r
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
r
4-pyridinecarboxaldehyde + NADPH
4-pyridinemethanol + NADP+
-
-
-
-
r
5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+
(furan-2,5-diyl)dimethanol + NADP+
-
-
-
-
?
5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+
(furan-2,5-diyl)dimethanol + NADP+
-
-
-
-
?
5-hydroxymethylfurfural + NADPH + H+
5-hydroxymethylfurfuryl alcohol + NADP+
-
-
-
?
5-hydroxymethylfurfural + NADPH + H+
5-hydroxymethylfurfuryl alcohol + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
the proposed physiological role of NADP-dependent alcohol dehydrogenase in Pyrococcus furiosus is to both dispose of excess reducing equivalents and to detoxify the aldehydes produced by the fermentative pathways
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 1% relative activity with 0.1 mM or 23% relative activity with 1 mM substrate
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
-
-
-
-
?
anisaldehyde + NADPH
4-methoxybenzyl alcohol + NADP+
-
72% of activity with cinnamaldehyde
-
-
r
anisaldehyde + NADPH
4-methoxybenzyl alcohol + NADP+
-
83% activity compared to cinnamaldehyde
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
8% of activity with acetaldehyde
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
45% activity compared to cinnamaldehyde
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
-
72% of activity with cinnamaldehyde
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH
-
45% of activity with cinnamyl alcohol
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH
-
78% activity compared to cinnamyl alcohol
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 42% relative activity with 1 mM or 79% relative activity with 10 mM substrate
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
42% activity with 1 mM benzyl alcohol, 79% activity with 10 mM benzyl alcohol
30% activity with 0.1 mM benzaldehyde, 14% activity with 1 mM benzaldehyde
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
180% of the activity with 2-propanol
-
-
?
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
-
180% of the activity with 2-propanol
-
-
?
butan-1-ol + NADP+
butanal + NADPH + H+
-
isoform ADH-I, 94%, isoform ADH II, 32% of the actvity with hexan-1-ol
-
-
?
butan-1-ol + NADP+
butanal + NADPH + H+
-
isoform ADH-I, 94%, isoform ADH II, 32% of the actvity with hexan-1-ol
-
-
?
butan-1-ol + NADP+
butanal + NADPH + H+
-
isoform AdhA, 238% of the activity with acetaldehyde
-
-
r
butan-2-ol + NADP+
butanone + NADPH + H+
-
isoform ADH-I, 89%, isoform ADH II, 23% of the actvity with hexan-1-ol
-
-
?
butan-2-ol + NADP+
butanone + NADPH + H+
-
isoform ADH-I, 89%, isoform ADH II, 23% of the actvity with hexan-1-ol
-
-
?
butanal + NADPH + H+
1-butanol + NADP+
47% of activity with acetaldehyde
-
-
r
butanal + NADPH + H+
1-butanol + NADP+
-
19% activity compared to cinnamaldehyde
-
-
r
butanal + NADPH + H+
1-butanol + NADP+
-
57% of activity with cinnamaldehyde
-
-
r
butanal + NADPH + H+
butan-1-ol + NADP+
-
isoform AdhA, 625%, isoform AdhB, 108% of the activity with acetaldehyde
-
-
r
butanol + NADP+
butanal + NADPH
68% of activity with ethanol
-
-
r
butanol + NADP+
butanal + NADPH
-
24% of activity with cinnamyl alcohol
-
-
r
butanol + NADP+
butanal + NADPH + H+
-
23% activity with 1 mM butanol, 70% activity with 10 mM butanol
73% activity with 0.1 mM butanal, 120% activity with 1 mM butanal
-
r
butanol + NADP+
butanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 23% relative activity with 1 mM or 70% relative activity with 10 mM substrate
-
-
r
cellobiose + NADP+
? + NADPH + H+
-
71% of the activity with 2-propanol
-
-
?
cellobiose + NADP+
? + NADPH + H+
-
71% of the activity with 2-propanol
-
-
?
cinnamyl alcohol + NADP+
cinnamaldehyde + NADPH + H+
-
100% activity with 1 mM cinnamyl alcohol, 116% activity with 10 mM hexanol
61% activity with 0.1 mM cinnamaldehyde, 7% activity with 1 mM cinnamaldehyde
-
r
cinnamyl alcohol + NADP+
cinnamaldehyde + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 100% relative activity with 1 mM or 116% relative activity with 10 mM substrate
-
-
r
coniferaldehyde + NADPH
coniferyl alcohol + NADP+
-
10% of activity with cinnamaldehyde
-
-
r
coniferaldehyde + NADPH
coniferyl alcohol + NADP+
-
15% activity compared to cinnamaldehyde
-
-
r
coniferaldehyde + NADPH
coniferyl alcohol + NADP+
-
converted less efficiently than eutypine
-
-
?
D-glucose + NADP+
? + NADPH + H+
-
146% of the activity with 2-propanol
-
-
?
D-glucose + NADP+
? + NADPH + H+
-
146% of the activity with 2-propanol
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
?
D-glucuronate + NADPH
L-gulonate + NADP+
-
-
-
-
r
D-glucuronate + NADPH + H+
?
0.1 M phosphate buffer, pH 7.0
-
-
?
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
r
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
-
-
ir
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
-
-
286165, 286167, 286168, 286171, 286172, 286174, 286176, 286177, 286178, 286179, 286181, 286182, 286196
-
-
r
decanol + NADP+
decanal + NADPH + H+
-
3% activity with 1 mM decanol, 6% activity with 10 mM decanol
21% activity with 0.1 mM decanal, 22% activity with 1 mM decanal
-
r
decanol + NADP+
decanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 3% relative activity with 1 mM or 6% relative activity with 10 mM substrate
-
-
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
kcat/Km for NAD+ is 1900fold lower compared to NADP+
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
isoform AdhB: no oxidation of ethanol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
kcat/Km for NAD+ is 1900fold lower compared to NADP+
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
2% activity compared to cinnamyl alcohol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
4% of activity with cinnamyl alcohol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
19% activity with 1 mM ethanol, 91% activity with 10 mM ethanol
1% activity with 0.1 mM acetaldehyde, 23% activity with 1 mM acetaldehyde
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 19% relative activity with 1 mM or 91% relative activity with 10 mM substrate
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
-
-
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
5.7% of the activity compared to the substrate 2-butanol
-
-
r
ethanol + NADP+
acetaldehyde + NADPH + H+
the native enzyme shows 100% activity with ethanol
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
the native enzyme shows 100% activity with ethanol
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl-4-chloro-3-oxobutanoate + NADPH + H+
ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl-4-chloro-3-oxobutanoate + NADPH + H+
ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethyl-4-chloro-3-oxobutanoate + NADPH + H+
ethyl-4-chloro-3-hydroxybutanoate + NADP+
-
-
-
-
?
ethylacetoacetate + NADPH
ethyl L-(+)-3-hydroxybutyrate + NADP+
-
-
-
-
?
formaldehyde + NADPH
methanol + NADP+
6% of activity with acetaldehyde
-
-
ir
formaldehyde + NADPH + H+
methanol + NADP+
-
-
-
?
furfural + NADH + H+
furfuryl alcohol + NAD+
-
-
-
?
furfural + NADPH
furfuryl alcohol + NADP+
-
27% of activity with cinnamaldehyde
-
-
r
furfural + NADPH
furfuryl alcohol + NADP+
-
54% activity compared to cinnamaldehyde
-
-
r
furfural + NADPH + H+
furfuryl alcohol + NADP+
-
-
-
?
furfural + NADPH + H+
furfuryl alcohol + NADP+
-
-
-
?
heptanal + NADPH
1-heptanol + NADP+
-
86% activity compared to cinnamaldehyde
-
-
r
heptanal + NADPH
1-heptanol + NADP+
-
88% of activity with cinnamaldehyde
-
-
r
hexanal + NADPH + H+
1-hexanol + NADP+
-
88% activity compared to cinnamaldehyde
-
-
r
hexanal + NADPH + H+
1-hexanol + NADP+
-
89% of activity with cinnamaldehyde
-
-
r
hexanal + NADPH + H+
hexanol + NADP+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 53% relative activity with 0.1 mM or 100% relative activity with 1 mM substrate
-
-
r
hexanol + NADP+
hexanal + NADPH
60% of activity with ethanol
-
-
r
hexanol + NADP+
hexanal + NADPH
-
154% of activity with cinnamyl alcohol
-
-
r
hexanol + NADP+
hexanal + NADPH + H+
-
27% activity with 1 mM hexanol, 40% activity with 10 mM hexanol
53% activity with 0.1 mM hexanal, 100% activity with 1 mM hexanal
-
r
hexanol + NADP+
hexanal + NADPH + H+
-
30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 27% relative activity with 1 mM or 40% relative activity with 10 mM substrate
-
-
r
isobutanol + NADP+
isobutanal + NADPH + H+
-
122% of the activity compared to ethanol
-
-
r
<