the oxidation of (S)-3-hydroxybutanoyl-CoA at pH 9.5 proceeds with 7% of the rate of the 3-acetoacetyl-CoA reduction. At pH 6.5, the reduction of 3-acetoacetyl-CoA is 14times faster than the oxidation of (S)-3-hydroxybutanoyl-CoA.The enzyme does not oxidize 3-hydroxyvaleryl-CoA or 3-hydroxycaproyl-CoA
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apo and NAD+-bound enzyme forms, hanging drop vapor diffusion method. NAD+-bound crystals grow using a reservoir solution consisting of 17.5% (w/v) PEG 3350, 200 mM sodium thiocyanate. Apo crystals grow using a reservoir solution consisting of 1.25% (w/v) PEG 400, 2.2 M ammonium sulfate, 0.1 M HEPES-NaOH pH 7.5